Hernan E Grecco

Summary

Country: Germany

Publications

  1. ncbi request reprint In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays
    Hernan E Grecco
    Max Planck Institute for Molecular Physiology, Department of Systemic Cell Biology, Dortmund, Germany
    Nat Methods 7:467-72. 2010
  2. ncbi request reprint Global analysis of time correlated single photon counting FRET-FLIM data
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Opt Express 17:6493-508. 2009
  3. doi request reprint FRET in cell biology: still shining in the age of super-resolution?
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, D 44227 Dortmund, Germany
    Chemphyschem 12:484-90. 2011
  4. pmc Optimizing cell arrays for accurate functional genomics
    Sven Fengler
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    BMC Res Notes 5:358. 2012
  5. doi request reprint Quantifying cellular dynamics by fluorescence resonance energy transfer (FRET) microscopy
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Curr Protoc Neurosci . 2013
  6. doi request reprint High-throughput quantification of posttranslational modifications in situ by CA-FLIM
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Methods Enzymol 500:37-58. 2011
  7. pmc The autodepalmitoylating activity of APT maintains the spatial organization of palmitoylated membrane proteins
    Nachiket Vartak
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Biophys J 106:93-105. 2014
  8. doi request reprint flatFLIM: enhancing the dynamic range of frequency domain FLIM
    Klaus C Schuermann
    Department of Systemic Cell Biology, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44221 Dortmund, Germany
    Opt Express 20:20730-41. 2012
  9. doi request reprint The GDI-like solubilizing factor PDEδ sustains the spatial organization and signalling of Ras family proteins
    Anchal Chandra
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto Hahn Str 11, 44227 Dortmund, Germany
    Nat Cell Biol 14:148-58. 2012
  10. doi request reprint Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics
    Eli Zamir
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
    Nat Methods 7:295-8. 2010

Collaborators

Detail Information

Publications11

  1. ncbi request reprint In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays
    Hernan E Grecco
    Max Planck Institute for Molecular Physiology, Department of Systemic Cell Biology, Dortmund, Germany
    Nat Methods 7:467-72. 2010
    ..Using FLIM on cell arrays (CA-FLIM), we uncovered components that transduce signals from epidermal growth factor receptor...
  2. ncbi request reprint Global analysis of time correlated single photon counting FRET-FLIM data
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Opt Express 17:6493-508. 2009
    ..The proposed method is efficient and robust, and can be routinely applied to analyze FRET-FLIM data acquired in intact cells...
  3. doi request reprint FRET in cell biology: still shining in the age of super-resolution?
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, D 44227 Dortmund, Germany
    Chemphyschem 12:484-90. 2011
    ..Herein we review the major applications in biological FRET imaging and we discuss the possibilities and challenges in the super-resolution era...
  4. pmc Optimizing cell arrays for accurate functional genomics
    Sven Fengler
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    BMC Res Notes 5:358. 2012
    ..Here we have developed a quality control protocol for quantifying and minimizing contamination in CA...
  5. doi request reprint Quantifying cellular dynamics by fluorescence resonance energy transfer (FRET) microscopy
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Curr Protoc Neurosci . 2013
    ..Here we describe how Förster/fluorescence resonance energy transfer (FRET) can be used to measure the phosphorylation state of proteins in the context of the cell...
  6. doi request reprint High-throughput quantification of posttranslational modifications in situ by CA-FLIM
    Hernan E Grecco
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Methods Enzymol 500:37-58. 2011
    ..After generating a single cell array containing 384 spots, it can be imaged and analyzed in less than 2h...
  7. pmc The autodepalmitoylating activity of APT maintains the spatial organization of palmitoylated membrane proteins
    Nachiket Vartak
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
    Biophys J 106:93-105. 2014
    ..The autodepalmitoylating activity on the Golgi constitutes a homeostatic regulation mechanism of APT levels at the Golgi that ensures robust partitioning of APT substrates between the plasma membrane and the Golgi. ..
  8. doi request reprint flatFLIM: enhancing the dynamic range of frequency domain FLIM
    Klaus C Schuermann
    Department of Systemic Cell Biology, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44221 Dortmund, Germany
    Opt Express 20:20730-41. 2012
    ..This improvement can be added to any FD-FLIM setup with minimal modifications, enhancing the throughput of information content...
  9. doi request reprint The GDI-like solubilizing factor PDEδ sustains the spatial organization and signalling of Ras family proteins
    Anchal Chandra
    Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto Hahn Str 11, 44227 Dortmund, Germany
    Nat Cell Biol 14:148-58. 2012
    ..Our findings link the activity of PDEδ in determining Ras protein topography to Ras-dependent signalling...
  10. doi request reprint Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics
    Eli Zamir
    Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
    Nat Methods 7:295-8. 2010
    ..With this approach, we analyzed the rapid physiological regulation of protein kinase A...
  11. doi request reprint Signaling from the living plasma membrane
    Hernan E Grecco
    Max Planck Institute for Molecular Physiology, Department of Systemic Cell Biology, Otto Hahn Str 11, D 44227 Dortmund, Germany
    Cell 144:897-909. 2011
    ..In this Review, we show how computer simulations can generate counterintuitive predictions about the spatial organization of these complex processes...