J Gieffers

Summary

Country: Germany

Publications

  1. pmc Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Appl Environ Microbiol 72:3785-7. 2006
  2. ncbi request reprint Phagocytes transmit Chlamydia pneumoniae from the lungs to the vasculature
    J Gieffers
    Institute for Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Eur Respir J 23:506-10. 2004
  3. ncbi request reprint Genotypic differences in the Chlamydia pneumoniae tyrP locus related to vascular tropism and pathogenicity
    Jens Gieffers
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Infect Dis 188:1085-93. 2003
  4. pmc In vitro susceptibilities of Chlamydia pneumoniae strains recovered from atherosclerotic coronary arteries
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    Antimicrob Agents Chemother 42:2762-4. 1998
  5. ncbi request reprint In vitro susceptibility and eradication of Chlamydia pneumoniae cardiovascular strains from coronary artery endothelium and smooth muscle cells
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Cardiovasc Drugs Ther 15:259-62. 2001
  6. ncbi request reprint Presence of Chlamydia pneumoniae DNA in the cerebral spinal fluid is a common phenomenon in a variety of neurological diseases and not restricted to multiple sclerosis
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Ann Neurol 49:585-9. 2001
  7. ncbi request reprint Cardiovascular infection by Chlamydia pneumoniae is not related to apolipoprotein E genotype
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    Clin Infect Dis 31:1515-6. 2000
  8. ncbi request reprint Atherogenetically relevant cells support continuous growth of Chlamydia pneumoniae
    M Maass
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Herz 25:68-72. 2000
  9. pmc Failure to detect Chlamydia pneumoniae in brain sections of Alzheimer's disease patients
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    J Clin Microbiol 38:881-2. 2000
  10. ncbi request reprint Poor correlation between microimmunofluorescence serology and polymerase chain reaction for detection of vascular Chlamydia pneumoniae infection in coronary artery disease patients
    M Maass
    Institute of Medical Microbiology, Medical University of Lubeck, Germany
    Med Microbiol Immunol 187:103-6. 1998

Collaborators

Detail Information

Publications25

  1. pmc Single-nucleotide-polymorphism-specific PCR for quantification and discrimination of Chlamydia pneumoniae genotypes by use of a "locked" nucleic acid
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Appl Environ Microbiol 72:3785-7. 2006
    ..Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP...
  2. ncbi request reprint Phagocytes transmit Chlamydia pneumoniae from the lungs to the vasculature
    J Gieffers
    Institute for Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Eur Respir J 23:506-10. 2004
    ..This is the first description of granulocytes acting as a reservoir for Chlamydia pneumoniae early in infection...
  3. ncbi request reprint Genotypic differences in the Chlamydia pneumoniae tyrP locus related to vascular tropism and pathogenicity
    Jens Gieffers
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Infect Dis 188:1085-93. 2003
    ..We hypothesize that the important difference between strains of vascular and respiratory origin may lie in the increased tendency of vascular strains to elicit persistent infection that is triggered by amino-acid starvation...
  4. pmc In vitro susceptibilities of Chlamydia pneumoniae strains recovered from atherosclerotic coronary arteries
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    Antimicrob Agents Chemother 42:2762-4. 1998
    ..Roxithromycin was the most active macrolide, and rifampin was the most effective drug overall...
  5. ncbi request reprint In vitro susceptibility and eradication of Chlamydia pneumoniae cardiovascular strains from coronary artery endothelium and smooth muscle cells
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Cardiovasc Drugs Ther 15:259-62. 2001
    ..pneumoniae from coronary endothelial and smooth muscle cells, celltypes that are involved in the pathogenesis of atherosclerosis...
  6. ncbi request reprint Presence of Chlamydia pneumoniae DNA in the cerebral spinal fluid is a common phenomenon in a variety of neurological diseases and not restricted to multiple sclerosis
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Ann Neurol 49:585-9. 2001
    ....
  7. ncbi request reprint Cardiovascular infection by Chlamydia pneumoniae is not related to apolipoprotein E genotype
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    Clin Infect Dis 31:1515-6. 2000
    ..pneumoniae infection of coronary artery tissue. The apoE genotype is not distinctly associated with an increased risk for vascular C. pneumoniae infection...
  8. ncbi request reprint Atherogenetically relevant cells support continuous growth of Chlamydia pneumoniae
    M Maass
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, Germany
    Herz 25:68-72. 2000
    ..Endovascular infection might provide an explanation for unclear phenomena of atherogenesis like mesenchymal cell proliferation and its distinct inflammatory component...
  9. pmc Failure to detect Chlamydia pneumoniae in brain sections of Alzheimer's disease patients
    J Gieffers
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, D 23538 Lubeck, Germany
    J Clin Microbiol 38:881-2. 2000
    ..pneumoniae-specific DNA nor chlamydial antigens. From our data, the presence of C. pneumoniae in the brains of Alzheimer's patients is not a common phenomenon; an association remains questionable...
  10. ncbi request reprint Poor correlation between microimmunofluorescence serology and polymerase chain reaction for detection of vascular Chlamydia pneumoniae infection in coronary artery disease patients
    M Maass
    Institute of Medical Microbiology, Medical University of Lubeck, Germany
    Med Microbiol Immunol 187:103-6. 1998
    ..Thus, the MIF test currently appears not suitable to predict individual vascular C. pneumoniae infection...
  11. ncbi request reprint Detection of Chlamydia pneumoniae within peripheral blood monocytes of patients with unstable angina or myocardial infarction
    M Maass
    Institute of Medical Microbiology and Hygiene, Medical University of Lubeck, 23538 Lubeck, Germany
    J Infect Dis 181:S449-51. 2000
    ..The potential role of chlamydiae in coronary atherosclerosis may therefore be more related to acceleration of disease or systemic effects by persistent infection than to sudden initiation of infarction by acute infection...
  12. ncbi request reprint Prevalence, genetic conservation and transmissibility of the Chlamydia pneumoniae bacteriophage (phiCpn1)
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, University of Lubeck, Lubeck, Germany
    FEMS Microbiol Lett 273:45-9. 2007
    ..pneumoniae with the phage provides the basis for studying its pathogenetic relevance in isolates of identical genetic background and provides a potential tool for genetic manipulation of C. pneumoniae...
  13. pmc Growth cycle-dependent pharmacodynamics of antichlamydial drugs
    Katrin Siewert
    Institute of Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Antimicrob Agents Chemother 49:1852-6. 2005
    ..These considerations provide important information on chlamydial biology and antimicrobial susceptibility. A combinational therapy of rifampin and a macrolide should be considered in therapy-refractory infections...
  14. pmc First-choice antibiotics at subinhibitory concentrations induce persistence of Chlamydia pneumoniae
    Jens Gieffers
    Institute of Medical Microbiology and Hygiene, University of Lubeck, 23538 Luebeck, Germany
    Antimicrob Agents Chemother 48:1402-5. 2004
    ..We found that all of the antibiotics tested have distinct and subinhibitory concentrations at which they induce persistence...
  15. ncbi request reprint Chlamydia pneumoniae multiply in neutrophil granulocytes and delay their spontaneous apoptosis
    Ger van Zandbergen
    Institute for Medical Microbiology and Hygiene, University of Lubeck, Lubeck, Germany
    J Immunol 172:1768-76. 2004
    ....
  16. pmc Isolation of Chlamydia pneumoniae clonal variants by a focus-forming assay
    Jens Gieffers
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Disease, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:5827-34. 2002
    ..These results now allow investigations on the biology and pathogenesis of C. pneumoniae clonal genovars that could lead to new insights into the pathogenesis of this important human pathogen...
  17. ncbi request reprint Variation in the mutation frequency determining quinolone resistance in Chlamydia trachomatis serovars L2 and D
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, University of Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany
    J Antimicrob Chemother 61:91-4. 2008
    ..Quinolone resistance of chlamydiae is supposed to be extremely rare. To assess the risk for the emergence of chlamydial quinolone resistance, we analysed the occurrence of resistant mutants in a quantitative perspective...
  18. ncbi request reprint Hydroxymethylglutaryl coenzyme A reductase inhibition reduces Chlamydia pneumoniae-induced cell interaction and activation
    Ralf Dechend
    Franz Volhard Clinic at the Max Delbrück Center for Molecular Medicine, HELIOS Klinikum Berlin, Wiltberg Strasse 50, 13125 Berlin, Germany
    Circulation 108:261-5. 2003
    ..Chlamydia pneumoniae stimulates chronic inflammation in vascular cells. Hydroxymethylglutaryl coenzyme A reductase inhibitors (statins) may have an ameliorating effect. We investigated possible mechanisms...
  19. ncbi request reprint Chlamydia pneumoniae and atherosclerosis
    Robert J Belland
    University of Tennessee Health Sciences Center, 858 Madison Avenue, Memphis, TN 38163, USA
    Cell Microbiol 6:117-27. 2004
    ..Genomic and proteomic analyses of C. pneumoniae infections in relevant cell types will help to define the pathogenic potential of the organism in both respiratory and cardiovascular disease...
  20. ncbi request reprint Micromanipulation of the Chlamydia pneumoniae inclusion: implications for cloning and host-pathogen interactions
    Jens Gieffers
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA
    FEMS Microbiol Lett 226:45-9. 2003
    ..We have used micromanipulation to clone C. pneumoniae and to free it from mycoplasma contamination...
  21. ncbi request reprint Cox-2 inhibition abrogates Chlamydia pneumoniae-induced PGE2 and MMP-1 expression
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, University of Luebeck, Germany
    Biochem Biophys Res Commun 320:738-44. 2004
    ....
  22. ncbi request reprint Recurrent optic neuritis associated with Chlamydia pneumoniae infection of the central nervous system
    Daniela Pohl
    Department of Paediatrics and Paediatric Neurology, Georg August University Gottingen, Germany
    Dev Med Child Neurol 48:770-2. 2006
    ..These findings suggest the possibility of a C. pneumoniae infection as a contributing factor or even causative event for the development of optic neuritis...
  23. ncbi request reprint Chlamydia pneumoniae directly interferes with HIF-1alpha stabilization in human host cells
    Jan Rupp
    Institute of Medical Microbiology and Hygiene, Center for Structural and Cell Biology in Medicine, University of Luebeck, 23538 Luebeck, Germany
    Cell Microbiol 9:2181-91. 2007
    ..Direct interference with HIF-1alpha stabilization was essential for efficient C. pneumoniae replication in hypoxia and highlights a novel strategy of adaptive pathogen-host interaction in chlamydial diseases...
  24. pmc Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by genome-wide analysis of single nucleotide polymorphisms: evidence for highly clonal population structure
    Thomas Rattei
    Institute of Medical Microbiology and Hygiene, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    BMC Genomics 8:355. 2007
    ..Four published genomes revealed an identity of > 99 %. This remarkable finding raised questions about the existence of distinguishable genotypes in correlation with geographical and anatomical origin...
  25. pmc Be aware of the possibility of false-positive results in single-locus PCR assays for methicillin-resistant Staphylococcus aureus
    Jan Rupp
    J Clin Microbiol 44:2317. 2006