S Wiemann

Summary

Affiliation: German Cancer Research Center
Country: Germany

Publications

  1. pmc RNAi-based validation of antibodies for reverse phase protein arrays
    Heiko A Mannsperger
    German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Proteome Sci 8:69. 2010
  2. pmc Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts
    Florian Hahne
    Division of Molecular Genome Analysis, German Cancer Research Center, INF 580, 69120 Heidelberg, Germany
    Genome Biol 7:R77. 2006
  3. pmc The full-ORF clone resource of the German cDNA Consortium
    Stephanie Bechtel
    Department of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    BMC Genomics 8:399. 2007
  4. pmc The systematic functional characterisation of Xq28 genes prioritises candidate disease genes
    Anja Kolb-Kokocinski
    Division of Molecular Genome Analysis, German Cancer Research Centre DKFZ, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Genomics 7:29. 2006
  5. pmc SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones
    Ruth Wellenreuther
    Department of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    BMC Genomics 5:36. 2004
  6. pmc Extending pathways based on gene lists using InterPro domain signatures
    Florian Hahne
    German Cancer Research Center, Molecular Genome Analysis, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Bioinformatics 9:3. 2008
  7. pmc The 3of5 web application for complex and comprehensive pattern matching in protein sequences
    Markus Seiler
    Division of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Bioinformatics 7:144. 2006
  8. pmc CAFTAN: a tool for fast mapping, and quality assessment of cDNAs
    Coral Del Val
    DKFZ, German Cancer Research Center, Division Molecular Biophysics, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    BMC Bioinformatics 7:473. 2006
  9. ncbi request reprint CDNAs for functional genomics and proteomics: the German Consortium
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    C R Biol 326:1003-9. 2003
  10. ncbi request reprint The German cDNA network: cDNAs, functional genomics and proteomics
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    J Struct Funct Genomics 4:87-96. 2003

Detail Information

Publications68

  1. pmc RNAi-based validation of antibodies for reverse phase protein arrays
    Heiko A Mannsperger
    German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Proteome Sci 8:69. 2010
    ..abstract:..
  2. pmc Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts
    Florian Hahne
    Division of Molecular Genome Analysis, German Cancer Research Center, INF 580, 69120 Heidelberg, Germany
    Genome Biol 7:R77. 2006
    ..The software is freely available in the Bioconductor package prada. The method permits analysis of large screens to detect the effects of molecular interventions in cellular systems...
  3. pmc The full-ORF clone resource of the German cDNA Consortium
    Stephanie Bechtel
    Department of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    BMC Genomics 8:399. 2007
    ..In combination with a unique and versatile cloning system, these will provide the tools for genome-wide systematic functional analyses, to achieve a deeper insight into complex biological processes...
  4. pmc The systematic functional characterisation of Xq28 genes prioritises candidate disease genes
    Anja Kolb-Kokocinski
    Division of Molecular Genome Analysis, German Cancer Research Centre DKFZ, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Genomics 7:29. 2006
    ..To expedite disease gene identification, we have initiated the functional characterisation of all known Xq28 genes...
  5. pmc SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones
    Ruth Wellenreuther
    Department of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    BMC Genomics 5:36. 2004
    ..The SMART technique for full-length enrichment is robust but results in limited cDNA insert size of the library...
  6. pmc Extending pathways based on gene lists using InterPro domain signatures
    Florian Hahne
    German Cancer Research Center, Molecular Genome Analysis, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Bioinformatics 9:3. 2008
    ..This works by comparing InterPro domain signatures of the candidate gene lists with domain signatures of gene sets derived from known classifications, e.g. KEGG pathways...
  7. pmc The 3of5 web application for complex and comprehensive pattern matching in protein sequences
    Markus Seiler
    Division of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Bioinformatics 7:144. 2006
    ..This is especially true for patterns containing ambiguous characters and positions and/or length ambiguities...
  8. pmc CAFTAN: a tool for fast mapping, and quality assessment of cDNAs
    Coral Del Val
    DKFZ, German Cancer Research Center, Division Molecular Biophysics, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    BMC Bioinformatics 7:473. 2006
    ..To this end we have developed a new high-throughput analysis tool, CAFTAN, which simplifies these efforts and thus fills the gap between large-scale cDNA collections and their systematic annotation and application in functional genomics...
  9. ncbi request reprint CDNAs for functional genomics and proteomics: the German Consortium
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    C R Biol 326:1003-9. 2003
    ..The resulting information is integrated with functional genomics and proteomics data for further understanding of protein functions in the cellular context...
  10. ncbi request reprint The German cDNA network: cDNAs, functional genomics and proteomics
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    J Struct Funct Genomics 4:87-96. 2003
    ....
  11. pmc Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, Heidelberg, 69120, Germany
    BMC Biol 3:16. 2005
    ..Tissue specific regulation of expression and splicing are major factors enhancing the number of the encoded products. Commonly these mechanisms are intragenic and affect only one gene...
  12. pmc From ORFeome to biology: a functional genomics pipeline
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg, Germany
    Genome Res 14:2136-44. 2004
    ..All information is presented in a Web database (http://www.dkfz.de/LIFEdb). It is exploited for the identification of disease-relevant genes and proteins for diagnosis and therapy...
  13. pmc Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs
    S Wiemann
    Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg, Germany
    Genome Res 11:422-35. 2001
    ..We thus substantially contribute to the generation of a gene catalog, consisting of both full-coding cDNA sequences and clones, which should be made freely available and will become an invaluable tool for detailed functional studies...
  14. ncbi request reprint DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer
    J Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Res 60:1704-10. 2000
    ..Thus, DMBT1 is a gene that is highly unstable in cancer and encodes for a protein with at least two different functions, one in the immune defense and a second one in epithelial differentiation...
  15. ncbi request reprint The genomic structure of the DMBT1 gene: evidence for a region with susceptibility to genomic instability
    J Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Oncogene 18:6233-40. 1999
    ..The major part of the gene harbours locus specific repeats. These repeats may point to the DMBT1 locus as a region susceptible to chromosomal instability...
  16. ncbi request reprint Transcription mapping in a medulloblastoma breakpoint interval and Smith-Magenis syndrome candidate region: identification of 53 transcriptional units and new candidate genes
    P Seranski
    Abt Molekulare Genomanalyse, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany
    Genomics 56:1-11. 1999
    ..Based on their homologies to known genes or proteins, some of the novel genes are considered candidate genes either for malignant diseases or for the Smith-Magenis syndrome...
  17. ncbi request reprint DelGEF, an RCC1-related protein encoded by a gene on chromosome 11p14 critical for two forms of hereditary deafness
    J Uhlmann
    Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    FEBS Lett 460:153-60. 1999
    ..In addition, we have identified a murine homologue, mDelGEF. The ubiquitously expressed soluble protein hDelGEF1 is found both in the cyytoplasm and in the nucleus. Overexpressed hDelGEF2 colocalizes with mitochondria...
  18. ncbi request reprint Deleted in Malignant Brain Tumors 1 is a versatile mucin-like molecule likely to play a differential role in digestive tract cancer
    J Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany
    Cancer Res 61:8880-6. 2001
    ..However, although DMBT1 originally has divergent functions in monolayered and multilayered epithelia, carcinogenesis possibly converges in a common pathway that requires an inactivation of its functions in the ECM...
  19. ncbi request reprint Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita
    N S Heiss
    Deutsches Krebsforschungszentrum DKFZ, Department of Molecular Genome Analysis, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Hum Mol Genet 8:2515-24. 1999
    ..Further, examination of dyskerin-EGFP fusions mimicking mutations detected in patients indicated that the intracellular mislocalization of dyskerin is unlikely to cause DKC...
  20. ncbi request reprint X-linked dyskeratosis congenita is caused by mutations in a highly conserved gene with putative nucleolar functions
    N S Heiss
    Deutsches Krebsforschungszentrum, Department of Molecular Genome Analysis, Heidelberg, Germany
    Nat Genet 19:32-8. 1998
    ..By analogy to the function of the known dyskerin orthologues, involvement in the cell cycle and nucleolar function is predicted for the protein...
  21. ncbi request reprint Frequent downregulation of DMBT1 and galectin-3 in epithelial skin cancer
    Jan Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Int J Cancer 105:149-57. 2003
    ..This would support the view that galectin-3 can exert tumor-suppressive effects in certain scenarios, and DMBT1/galectin-3-mediated differentiation represents a candidate mechanism for this effect...
  22. ncbi request reprint Genomic organization of a 225-kb region in Xq28 containing the gene for X-linked myotubular myopathy (MTM1) and a related gene (MTMR1)
    P Kioschis
    Deutsches Krebsforschungszentrum, Molekulare Genomanalyse, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany
    Genomics 54:256-66. 1998
    ..Knowledge of the genomic sequence will facilitate mutation analyses of the coding and noncoding sequences of MTM1 and MTMR1...
  23. ncbi request reprint Pore membrane and/or filament interacting like protein 1 (POMFIL1) is predominantly expressed in the nervous system and encodes different protein isoforms
    Johannes F Coy
    German Cancer Research Center, Division of Molecular Genomeanalysis, Im Neuenheimer Feld 280, Heidelberg, Germany
    Gene 290:73-94. 2002
    ....
  24. ncbi request reprint Re-expression of microRNA-375 reverses both tamoxifen resistance and accompanying EMT-like properties in breast cancer
    A Ward
    Division of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    Oncogene 32:1173-82. 2013
    ..As most cancer-related deaths occur because of resistance to standard therapies and metastasis, re-expression of miR-375 or targeting MTDH might serve as potential therapeutic approaches for the treatment of TamR breast cancer...
  25. ncbi request reprint miR-200bc/429 cluster targets PLCgamma1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer
    S Uhlmann
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Oncogene 29:4297-306. 2010
    ..Our results suggest that the miR-200 family has a tumor-suppressor function by negatively regulating EGF-driven cell invasion, viability and cell cycle progression in breast cancer...
  26. ncbi request reprint DMBT1, a new member of the SRCR superfamily, on chromosome 10q25.3-26.1 is deleted in malignant brain tumours
    J Mollenhauer
    Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nat Genet 17:32-9. 1997
    ..Lack of DMBT1 expression has been demonstrated in 4/5 brain-tumour cell lines. We suggest that DMBT1 is a putative tumour-suppressor gene implicated in the carcinogenesis of medulloblastoma and glibolastoma multiforme...
  27. ncbi request reprint Construction of a physical map of an autism susceptibility region in 7q32.3-q33
    K S Beyer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Gene 272:85-91. 2001
    ..As this map contains a putative autistic disorder locus this integrated physical and transcript map provides a valuable resource for identification of candidate gene(s)...
  28. pmc Primer design for large scale sequencing
    S Haas
    Department of Molecular Genome Analysis and Department of Theoretical Bioinformatics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nucleic Acids Res 26:3006-12. 1998
    ..The high performance of PRIDE, combined with its minimal requirement of user interaction and its fast algorithm, make this program useful for the large scale design of primers, especially in large sequencing projects...
  29. ncbi request reprint Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells
    M Sauermann
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Oncogene 27:1320-6. 2008
    ..In conclusion, our findings establish Vmp1 to be a novel cell-cell adhesion protein and that its expression level determines the invasion and metastatic potential of cancer cells...
  30. ncbi request reprint Isolation of human and mouse HMG2a cDNAs: evidence for an HMG2a-specific 3' untranslated region
    K Wilke
    Deutsches Krebsforschungszentrum, Abteilung Molekulare Genomanalyse, Heidelberg, Germany
    Gene 198:269-74. 1997
    ..Whereas expression of the chicken HMG2a gene has previously been demonstrated in liver of newly hatched chicken, the human HMG2a gene is transcribed mainly in placenta...
  31. pmc Dynamic deterministic effects propagation networks: learning signalling pathways from longitudinal protein array data
    Christian Bender
    Department of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Bioinformatics 26:i596-602. 2010
    ....
  32. pmc KEGGgraph: a graph approach to KEGG PATHWAY in R and bioconductor
    Jitao David Zhang
    Division of Molecular Genome Analysis, German Cancer Research Center DKFZ, INF 580, Heidelberg, Germany
    Bioinformatics 25:1470-1. 2009
    ..While valuable graph tools have been implemented in R/Bioconductor, to our knowledge there is currently no software package to parse and analyze KEGG pathways with graph theory...
  33. doi request reprint QuantProReloaded: quantitative analysis of microspot immunoassays
    Anika Jöcker
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Bioinformatics 26:2480-1. 2010
    ..Availability and implementation: QuantProReloaded is written in R and Java and is open for download under the BSB license at http://code.google.com/p/quantproreloaded/...
  34. pmc Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification
    Jan C Brase
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Proteome Sci 8:36. 2010
    ..Increasing the sensitivity of RPPA approaches is important since many signaling proteins or posttranslational modifications are present at a low level...
  35. ncbi request reprint Modeling breast cell cycle regulation--overcoming drug resistance
    Dorit Arlt
    Div of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Conf Proc IEEE Eng Med Biol Soc 1:40-3. 2006
  36. pmc The LIFEdb database in 2006
    Alexander Mehrle
    Division Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 34:D415-8. 2006
    ..LIFEdb enables researchers to systematically select and characterize genes and proteins of interest, and presents data and information via its user-friendly web-based interface...
  37. ncbi request reprint Protein microarrays as a discovery tool for studying protein-protein interactions
    Ulrike Korf
    Division of Molecular Genome Analysis B050, DKFZ Heidelberg, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Expert Rev Proteomics 2:13-26. 2005
    ..This review provides an overview on the basic principles of protein microarrays and their potential to multiplex protein-protein interaction studies...
  38. ncbi request reprint The SRCR/SID region of DMBT1 defines a complex multi-allele system representing the major basis for its variability in cancer
    Jan Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Genes Chromosomes Cancer 35:242-55. 2002
    ..DMBT1 thus compares to mucins rather than to conventional tumor suppressors...
  39. pmc High-throughput protein analysis integrating bioinformatics and experimental assays
    Coral Del Val
    Division of Molecular Biophysics, German Cancer Research Center DKFZ, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 32:742-8. 2004
    ....
  40. ncbi request reprint An integrative model on the role of DMBT1 in epithelial cancer
    Jan Mollenhauer
    Cancer Detect Prev 26:266-74. 2002
    ..The expression patterns in carcinomas arising from the respective structures support this view. Accepting this functional dualism gives rise to an initial model on the role of DMBT1 in epithelial cancer...
  41. pmc LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system
    Detlev Bannasch
    German Cancer Research Center DKFZ, Division of Molecular Genome Analysis, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 32:D505-8. 2004
    ..A web interface enables the scientific community to explore and visualize features of the annotated cDNAs and ORFs combined with experimental results, and thus helps to unravel new features of proteins with as yet unknown functions...
  42. ncbi request reprint Sequential changes of the DMBT1 expression and location in normal lung tissue and lung carcinomas
    Jan Mollenhauer
    Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Genes Chromosomes Cancer 35:164-9. 2002
    ..We hypothesize that the changes of the DMBT1 expression and location do reflect a time course that may point to possible mechanisms for its role in epithelial cancer...
  43. ncbi request reprint Large-scale protein expression for proteome research
    Ulrike Korf
    Molecular Genome Analysis, DKFZ, Heidelberg, Germany
    Proteomics 5:3571-80. 2005
    ..Affinity-purified fusion proteins were confirmed by MALDI-TOF MS...
  44. doi request reprint Functional genomics and proteomics approaches to study the ERBB network in cancer
    Ozgur Sahin
    Division of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    FEBS Lett 583:1766-71. 2009
    ..Here, we describe the potential of applying functional genomics and proteomics, taking the ERBB family of growth-factor receptors as an example to study the signaling network and its impact on cancer...
  45. doi request reprint MicroRNA-520/373 family functions as a tumor suppressor in estrogen receptor negative breast cancer by targeting NF-κB and TGF-β signaling pathways
    I Keklikoglou
    Division of Molecular Genome Analysis, German Cancer Research Center DKFZ, Heidelberg, Germany
    Oncogene 31:4150-63. 2012
    ....
  46. pmc Combinatorial RNAi for quantitative protein network analysis
    Ozgur Sahin
    Division of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 104:6579-84. 2007
    ..This experimental approach combines multiple gene knockdown with a subsequent quantitative validation of reduced protein expression and is a major advancement toward the analysis of signaling pathways in systems biology...
  47. pmc Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance
    Ozgur Sahin
    German Cancer Research Center, Heidelberg, Germany
    BMC Syst Biol 3:1. 2009
    ....
  48. doi request reprint Contact spotting of protein microarrays coupled with spike-in of normalizer protein permits time-resolved analysis of ERBB receptor signaling
    Christian Löbke
    Division of Molecular Genome Analysis, German Cancer Research Center Heidelberg, Heidelberg, Germany
    Proteomics 8:1586-94. 2008
    ....
  49. ncbi request reprint Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle
    Dorit Arlt
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Cancer Res 65:7733-42. 2005
    ..We have established a novel functional profiling strategy that links genomics to cell biology and showed its potential for discerning cancer relevant modulators of the cell cycle in the candidate lists from microarray studies...
  50. ncbi request reprint Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX
    H Voss
    Biological Structures and Biocomputing Programmes, European Molecular Biology Laboratory, Heidelberg, Germany
    Yeast 11:61-78. 1995
    ..A surprisingly large fraction of the ORFs (at least six out of 17) in the centromeric region are apparently involved in RNA or DNA binding...
  51. ncbi request reprint Infrared-based protein detection arrays for quantitative proteomics
    Christian Loebke
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Proteomics 7:558-64. 2007
    ..Employing the IPAQ approach, a detailed analysis of activated signaling networks in biopsy samples and of crosstalk between signaling modules as required in drug discovery strategies can easily be performed...
  52. pmc Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
    Veronika E Neubrand
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    EMBO J 24:1122-33. 2005
    ..This suggests that the direct interaction of 2c18 and gamma1-adaptin is crucial for membrane association and thus the function of the AP-1 complex in living cells. We propose to name this protein gamma-BAR...
  53. ncbi request reprint Transcriptional analysis of the candidate region for incontinentia pigmenti (IP2) in Xq28
    U C Rogner
    Deutsches Krebsforschungszentrum, Abteilung Molekulare Genomanalyse, Heidelberg, Germany
    Genome Res 6:922-34. 1996
    ....
  54. ncbi request reprint An anthropoid-specific segmental duplication on human chromosome 1q22
    Vladimir Yu Kuryshev
    Department of Molecular Genome Analysis, DKFZ German Cancer Research Center, INF 580, D 69120 Heidelberg, Germany
    Genomics 88:143-51. 2006
    ..Moreover, we provide detailed information regarding structure and evolution of the SD1q22 region that is a prerequisite for future studies of its anthropoid-specific functions and possible linkage to human genetic disorders...
  55. ncbi request reprint Cloning of the C alpha catalytic subunit of the bovine cAMP-dependent protein kinase
    S Wiemann
    Biochemical Instrumentation, European Molecular Biology Laboratory, Heidelberg, Germany
    Biochim Biophys Acta 1171:93-6. 1992
    ..The C alpha mRNA was found to be expressed differentially in various bovine tissues...
  56. ncbi request reprint Systematic comparison of surface coatings for protein microarrays
    Birgit Guilleaume
    Department of Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg, Germany
    Proteomics 5:4705-12. 2005
    ..In summary, we demonstrate that only suitable combinations of surface and fluorescent dyes allow the generation of highly reproducible data...
  57. pmc Integrative annotation of 21,037 human genes validated by full-length cDNA clones
    Tadashi Imanishi
    Integrated Database Group, Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan
    PLoS Biol 2:e162. 2004
    ..The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology...
  58. pmc Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project
    Chris F Taylor
    European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK
    Nat Biotechnol 26:889-96. 2008
  59. pmc The H-Invitational Database (H-InvDB), a comprehensive annotation resource for human genes and transcripts
    Chisato Yamasaki
    Japan Biological Information Research Center, Japan Biological Informatics Consortium, Japan
    Nucleic Acids Res 36:D793-9. 2008
    ....
  60. ncbi request reprint Proteomics and Beyond: a report on the 3rd Annual Spring Workshop of the HUPO-PSI 21-23 April 2006, San Francisco, CA, USA
    Sandra Orchard
    EMBL Outstation European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, UK
    Proteomics 6:4439-43. 2006
    ....
  61. pmc Large-scale identification and characterization of alternative splicing variants of human gene transcripts using 56,419 completely sequenced and manually annotated full-length cDNAs
    Jun Ichi Takeda
    Integrated Database Group, Japan Biological Information Research Center, Japan Biological Informatics Consortium, AIST Bio IT Research, Building Aomi 2 42, Koto ku, Tokyo 135 0064, Japan
    Nucleic Acids Res 34:3917-28. 2006
    ....
  62. pmc High-content microscopy identifies new neurite outgrowth regulators
    Vibor Laketa
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory Heidelberg, 69117 Heidelberg, Germany
    Mol Biol Cell 18:242-52. 2007
    ..Altogether, we demonstrate the potential of the systematic microscope-based screening approaches described here to tackle the complex biological process of neurite outgrowth regulation...
  63. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
    ..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
  64. doi request reprint Human Proteinpedia enables sharing of human protein data
    Suresh Mathivanan
    Nat Biotechnol 26:164-7. 2008
  65. ncbi request reprint The first RSBI (ISA-TAB) workshop: "can a simple format work for complex studies?"
    Susanna Assunta Sansone
    EMBL EBI The European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    OMICS 12:143-9. 2008
    ..This article is part of the special issue of OMICS on the activities of the Genomics Standards Consortium (GSC)...
  66. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
    ..It will also serve as an example for similar microscope-based screens addressing different biological questions...
  67. ncbi request reprint PML-associated repressor of transcription (PAROT), a novel KRAB-zinc finger repressor, is regulated through association with PML nuclear bodies
    Sandra Fleischer
    Heinrich Pette Institut für Experimentelle Virologie und Immunologie, Martinistrasse 52, 20251 Hamburg, Germany
    Exp Cell Res 312:901-12. 2006
    ..Taken together, our data identify a novel transcriptional repressor and provide evidence for its regulation through association with PML-NBs...
  68. pmc Integrating systems biology with clinical research
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 9:314. 2008
    ..A report on the conference 'Systems Genomics 2008', Heidelberg, Germany, 2-3 May 2008...