Andreas Thomas

Summary

Affiliation: German Sport University
Country: Germany

Publications

  1. doi request reprint Quantification of urinary AICAR concentrations as a matter of doping controls
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2899-908. 2010
  2. doi request reprint Mass spectrometric determination of gonadotrophin-releasing hormone (GnRH) in human urine for doping control purposes by means of LC-ESI-MS/MS
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50993 Cologne, Germany
    J Mass Spectrom 43:908-15. 2008
  3. doi request reprint Determination of Synacthen in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:2669-74. 2009
  4. ncbi request reprint Quantification of AICAR-ribotide concentrations in red blood cells by means of LC-MS/MS
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933, Cologne, Germany
    Anal Bioanal Chem 405:9703-9. 2013
  5. doi request reprint Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Chromatogr A 1259:251-7. 2012
  6. doi request reprint Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Methods 56:230-5. 2012
  7. doi request reprint Sensitive determination of prohibited drugs in dried blood spots (DBS) for doping controls by means of a benchtop quadrupole/Orbitrap mass spectrometer
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 403:1279-89. 2012
  8. doi request reprint Sensitive and fast identification of urinary human, synthetic and animal insulin by means of nano-UPLC coupled with high-resolution/high-accuracy mass spectrometry
    Andreas Thomas
    Institute of Biochemistry Centre for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 1:219-27. 2009
  9. doi request reprint Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Drug Test Anal 2:144-8. 2010
  10. doi request reprint Determination of growth hormone releasing peptides (GHRP) and their major metabolites in human urine for doping controls by means of liquid chromatography mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:507-16. 2011

Collaborators

Detail Information

Publications67

  1. doi request reprint Quantification of urinary AICAR concentrations as a matter of doping controls
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2899-908. 2010
    ..100%), limit of quantification (100 ng/mL), stability and ion suppression effects, employing an in-house synthesised (13)C(5)-labelled AICAR as internal standard...
  2. doi request reprint Mass spectrometric determination of gonadotrophin-releasing hormone (GnRH) in human urine for doping control purposes by means of LC-ESI-MS/MS
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50993 Cologne, Germany
    J Mass Spectrom 43:908-15. 2008
    ..5 pg/ml), precision (inter/intraday, < 20%) and accuracy (105%) using Des-pGlu(1)-GnRH as internal standard to control each sample preparation step...
  3. doi request reprint Determination of Synacthen in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:2669-74. 2009
    ..For all experiments, the ACTH fragment 1-17 was used as the internal standard...
  4. ncbi request reprint Quantification of AICAR-ribotide concentrations in red blood cells by means of LC-MS/MS
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933, Cologne, Germany
    Anal Bioanal Chem 405:9703-9. 2013
    ....
  5. doi request reprint Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Chromatogr A 1259:251-7. 2012
    ..The proof-of-principle was shown by analysing excretion study urine samples for LHRH, Desmopressin and GHRP-2...
  6. doi request reprint Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Methods 56:230-5. 2012
    ..is enabled...
  7. doi request reprint Sensitive determination of prohibited drugs in dried blood spots (DBS) for doping controls by means of a benchtop quadrupole/Orbitrap mass spectrometer
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 403:1279-89. 2012
    ..Six isotope-labelled analogues used as internal standards facilitate a quantitative result interpretation which is of utmost importance especially for in-competition drug sports testing...
  8. doi request reprint Sensitive and fast identification of urinary human, synthetic and animal insulin by means of nano-UPLC coupled with high-resolution/high-accuracy mass spectrometry
    Andreas Thomas
    Institute of Biochemistry Centre for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 1:219-27. 2009
    ..The method was fully validated for specificity, limit of detection (LOD, 0.5 fmol/mL), precision (<20%), recovery (approximately 30%) and linearity (2-40 fmol/mL) for all target analytes...
  9. doi request reprint Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Drug Test Anal 2:144-8. 2010
    ..Hereby, the content of GHRP-2 was determined with approximately 50 µg per tablet...
  10. doi request reprint Determination of growth hormone releasing peptides (GHRP) and their major metabolites in human urine for doping controls by means of liquid chromatography mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:507-16. 2011
    ..Here, the known metabolite was detectable over 20 h after administration while the intact drug was not observed...
  11. doi request reprint Determination of Vasopressin and Desmopressin in urine by means of liquid chromatography coupled to quadrupole time-of-flight mass spectrometry for doping control purposes
    A Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Chim Acta 707:107-13. 2011
    ..The general requirements of the developed method provide the characteristics for an easy transfer to other anti-doping laboratories and support closing another potential gap for cheating athletes...
  12. doi request reprint Comprehensive plasma-screening for known and unknown substances in doping controls
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 24:1124-32. 2010
    ..1-10 ng/mL), precision (9-28%), robustness, linearity, ion suppression and recovery (80-112%). In addition to the identification of unknown compounds, the plasma samples were simultaneously screened for known prohibited targets...
  13. ncbi request reprint Determination of selected stimulants in urine for sports drug analysis by solid phase extraction via cation exchange and means of liquid chromatography-tandem mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:135-43. 2008
    ..This method provides a rugged and highly specific alternative to the established method utilising gas or liquid chromatography after liquid-liquid extraction...
  14. doi request reprint Determination of IGF-1 and IGF-2, their degradation products and synthetic analogues in urine by LC-MS/MS
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Analyst 136:1003-12. 2011
    ..The method was validated for qualitative purposes considering the parameters specificity, limit of detection (20-50 pg mL(-1)), recovery (10-35%), precision (<20%), linearity, robustness and stability...
  15. doi request reprint Mass spectrometric detection of peginesatide in human urine in doping control analysis
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Pharm Biomed Anal 70:512-7. 2012
    ..v. administration of peginesatide over a period of 4 days...
  16. doi request reprint Trafficking of drug candidates relevant for sports drug testing: detection of non-approved therapeutics categorized as anabolic and gene doping agents in products distributed via the Internet
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 3:331-6. 2011
    ....
  17. doi request reprint Screening for benfluorex and its major urinary metabolites in routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:543-51. 2011
    ....
  18. doi request reprint Characterization of two major urinary metabolites of the PPARdelta-agonist GW1516 and implementation of the drug in routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2479-91. 2010
    ..Validation was performed for specificity, limit of detection (0.1 ng/ml), recovery (72%), intraday and interday precisions (7.7-15.1%), and ion suppression/enhancement effects (<10%)...
  19. doi request reprint Mass spectrometric characterization of urinary metabolites of the selective androgen receptor modulator S-22 to identify potential targets for routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Rapid Commun Mass Spectrom 25:2187-95. 2011
    ..The obtained data supports future efforts to effectively screen for and confirm the misuse of the non-approved S-22 drug candidate in doping controls...
  20. ncbi request reprint Effects of gamma irradiation and 15 days of subsequent ex vivo storage on the cytosolic red blood cell proteome analyzed by 2D-DIGE and Orbitrap MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Proteomics Clin Appl 7:561-70. 2013
    ..So far, the effects of ionizing radiation on RBC structure and function and especially the proteome are not fully understood...
  21. doi request reprint Storage-induced changes of the cytosolic red blood cell proteome analyzed by 2D DIGE and high-resolution/high-accuracy MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Proteomics 12:3263-72. 2012
    ..These can serve as a basis for the development of a screening assay to detect RBC storage lesions and autologous blood doping in sports...
  22. ncbi request reprint Mass spectrometry-based characterization of new drugs and methods of performance manipulation in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 16:301-12. 2010
    ....
  23. doi request reprint Electron ionization mass spectrometry of the ryanodine receptor-based Ca(2+)-channel stabilizer S-107 and its implementation into routine doping control
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:2363-70. 2009
    ..5-17.2%), as well as recovery (63-66%) were determined. The established procedure proved suitable for routine doping control analysis to detect a potential misuse of the drug candidate S-107 in elite sport...
  24. doi request reprint Doping control analysis of emerging drugs in human plasma - identification of GW501516, S-107, JTV-519, and S-40503
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:1139-46. 2009
    ..The method was validated with regard to specificity, limits of detection (0.4-8.3 ng/mL), recoveries (72-98%), intraday and interday precisions (12-21%), and ion suppression/enhancement effects...
  25. doi request reprint Proteolysis and autolysis of proteases and the detection of degradation products in doping control
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 1:81-6. 2009
    ..All experiments were performed with fortified urine samples under consideration of assumed realistic conditions for adulteration by cheating sportsmen...
  26. doi request reprint Screening for the synthetic cannabinoid JWH-018 and its major metabolites in human doping controls
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 3:609-20. 2011
    ..The method was applied to approximately 7500 urine doping control samples yielding two JWH-018 findings and demonstrated its capability for a sensitive and selective identification of JWH-018 and its metabolites in human urine...
  27. doi request reprint Confiscated black market products and nutritional supplements with non-approved ingredients analyzed in the Cologne Doping Control Laboratory 2009
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 2:533-7. 2010
    ..The awareness of new products on the black market and in nutritional supplements is of utmost importance for laboratories to develop detection methods accordingly and screen for new substances as early as possible...
  28. doi request reprint Doping control analysis of tricyclic tetrahydroquinoline-derived selective androgen receptor modulators using liquid chromatography/electrospray ionization tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 22:2471-8. 2008
    ..The compounds were implemented into existing screening procedures, and detection limits (0.2-0.6 ng/mL), recoveries (92-97%), and intraday and interday precision (<22%) were evaluated...
  29. doi request reprint Application of FAIMS to anabolic androgenic steroids in sport drug testing
    Sven Guddat
    Institute of Biochemistry, German Sport University, Cologne, Germany
    Drug Test Anal 1:545-53. 2009
    ..The assay was applied to doping-control samples. For all analytes, LC-FAIMS-MS/MS resulted in excellent interference removal, which effectively extends the post-dose detection time...
  30. doi request reprint Mass spectrometric characterization of urinary metabolites of the selective androgen receptor modulator andarine (S-4) for routine doping control purposes
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 24:2245-54. 2010
    ..The obtained data will support future efforts to effectively screen for and confirm the misuse of the non-approved drug candidate Andarine...
  31. doi request reprint Insulins in equine urine: qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse-racing
    Tiia Kuuranne
    Institute of Biochemistry Center for Preventive Doping Research, German Sports University Cologne, Cologne, Germany
    Rapid Commun Mass Spectrom 22:355-62. 2008
    ....
  32. doi request reprint Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Electrophoresis 33:2537-45. 2012
    ....
  33. doi request reprint Metabolism of growth hormone releasing peptides
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University, Cologne, Germany
    Anal Chem 84:10252-9. 2012
    ..All identified metabolites were formed due to exopeptidase- (amino- or carboxy-), amidase-, or endopeptidase activity...
  34. doi request reprint Detection of peginesatide in equine serum using liquid chromatography-tandem mass spectrometry for doping control purposes
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 18:407-12. 2012
    ....
  35. doi request reprint Structure characterisation of urinary metabolites of the cannabimimetic JWH-018 using chemically synthesised reference material for the support of LC-MS/MS-based drug testing
    Simon Beuck
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:493-505. 2011
    ....
  36. doi request reprint Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 25:2115-23. 2011
    ..Its fitness for purpose was evaluated by the determination of selected method characteristics focusing particularly on specificity, recovery (ca. 60%), and limit of detection (1 ng/mL)...
  37. doi request reprint Characterization of in vitro generated metabolites of the selective androgen receptor modulators S-22 and S-23 and in vivo comparison to post-administration canine urine specimens
    Mario Thevis
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 2:589-98. 2010
    ..4 µg/kg) dosing. Finally, M3 was chemically synthesized, characterized by nuclear magnetic resonance spectroscopy and high resolution/high accuracy mass spectrometry, and chosen as primary target for future doping control analyses...
  38. ncbi request reprint Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Chem 78:1897-903. 2006
    ..A reliable tool is provided that allows the qualitative determination of rapid-acting insulins in urine specimens collected for sports drug testing...
  39. doi request reprint Screening for the calstabin-ryanodine receptor complex stabilizers JTV-519 and S-107 in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, 50933 Cologne, Germany
    Drug Test Anal 1:32-42. 2009
    ..Validation items such as specificity, recovery (68-92%), lower limit of detection (0.1-0.2 ng/mL), intraday (5.2-18.5%) and interday (8.7-18.8%) precision as well as ion suppression/enhancement effects were determined...
  40. doi request reprint Emerging drugs: mechanism of action, mass spectrometry and doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Mass Spectrom 44:442-60. 2009
    ..Compounds such as ryanodine-calstabin-complex modulators (benzothiazepines) are included, their mass spectrometric properties discussed, and current approaches in sports drug testing outlined...
  41. ncbi request reprint Isotope-dilution mass spectrometric quantification of the prodrug lisdexamfetamine in human urine in doping control analysis
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Rapid Commun Mass Spectrom 28:781-6. 2014
    ....
  42. doi request reprint Traditional Chinese medicine and sports drug testing: identification of natural steroid administration in doping control urine samples resulting from musk (pod) extracts
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Br J Sports Med 47:109-14. 2013
    ....
  43. ncbi request reprint Mass spectrometric identification and characterization of a new long-term metabolite of metandienone in human urine
    Wilhelm Schanzer
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:2252-8. 2006
    ....
  44. doi request reprint Effects of endurance exercise on the urinary proteome analyzed by 2-D PAGE and Orbitrap MS
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Proteomics Clin Appl 4:568-76. 2010
    ..Usually, total protein or albumin was measured for diagnosis of a proteinuria, and the present study was performed to search for qualitative differences in the urinary proteome before and after endurance exercise...
  45. doi request reprint Combination of carbon isotope ratio with hydrogen isotope ratio determinations in sports drug testing
    Thomas Piper
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Koln, Germany
    Anal Bioanal Chem 405:5455-66. 2013
    ..The combination of both stable isotopes would allow for lower reference limits providing the same statistical power and certainty to distinguish between the endo- or exogenous origin of a urinary steroid...
  46. doi request reprint Development and validation of a mass spectrometric detection method of peginesatide in dried blood spots for sports drug testing
    Ines Möller
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 403:2715-24. 2012
    ....
  47. doi request reprint Identification of fibroblast growth factor 1 (FGF-1) in a black market product
    Katja Walpurgis
    Centre for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Drug Test Anal 3:791-7. 2011
    ..In that regard, physiological concentrations of blood and urine specimens collected from healthy individuals were analyzed and were found to range below 28 pg/ml in urine, while there was no detectable FGF-1 in plasma...
  48. doi request reprint Doping control analysis of selected peptide hormones using LC-MS(/MS)
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Forensic Sci Int 213:35-41. 2011
    ....
  49. doi request reprint Doping control analysis of metamfepramone and two major metabolites using liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 15:507-15. 2009
    ..Although the active drug was not detected, the indicative metabolites methylpseudoephedrine and methcathinone were considered sufficient to infer the application of the prohibited drug...
  50. ncbi request reprint Mass spectrometric identification of degradation products of insulin and its long-acting analogues in human urine for doping control purposes
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Chem 79:2518-24. 2007
    ....
  51. ncbi request reprint Mass spectrometric determination of insulins and their degradation products in sports drug testing
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, Cologne, Germany
    Mass Spectrom Rev 27:35-50. 2008
    ..Diagnostic product ions and analytical strategies are presented and discussed in light of the need to unambiguously identify misused drugs in urine and plasma specimens for doping control...
  52. doi request reprint Determination of benzimidazole- and bicyclic hydantoin-derived selective androgen receptor antagonists and agonists in human urine using LC-MS/MS
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933, Cologne, Germany
    Anal Bioanal Chem 391:251-61. 2008
    ..4 and 13.2% and between 6.5 and 24.2%, respectively. Recoveries varied from 89 to 106%, and tests for ion suppression or enhancement effects were negative for all analytes. [figure: see text]..
  53. ncbi request reprint Determination of ¹³C/¹² C ratios of endogenous urinary 5-amino-imidazole-4-carboxamide 1β-D-ribofuranoside (AICAR)
    Thomas Piper
    German Sport University Cologne, Center for Preventive Doping Research Institute of Biochemistry, Am Sportpark Müngersdorf 6, 50933, Koln, Germany
    Rapid Commun Mass Spectrom 28:1194-202. 2014
    ..Therefore, a method for the determination of carbon isotope ratios (CIRs) of urinary AICAR has been developed and validated...
  54. doi request reprint Detection of small interfering RNA (siRNA) by mass spectrometry procedures in doping controls
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Drug Test Anal 5:853-60. 2013
    ..Simultaneously collected blood samples yielded no promising results. The methods were validated and found fit-for-purpose for doping controls...
  55. doi request reprint Analysis of octopamine in human doping control samples
    Mario Thevis
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Biomed Chromatogr 26:610-5. 2012
    ....
  56. ncbi request reprint Qualitative determination of synthetic analogues of insulin in human plasma by immunoaffinity purification and liquid chromatography-tandem mass spectrometry for doping control purposes
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Chem 77:3579-85. 2005
    ..Recoveries of synthetic insulins from plasma aliquots ranged from 91 to 98%, and detection limits were accomplished at 0.5 ng/mL for all target analytes...
  57. doi request reprint Structure elucidation of the diagnostic product ion at m/z 97 derived from androst-4-en-3-one-based steroids by ESI-CID and IRMPD spectroscopy
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    J Am Soc Mass Spectrom 23:537-46. 2012
    ....
  58. doi request reprint Current role of LC-MS(/MS) in doping control
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:405-20. 2011
    ....
  59. doi request reprint Detection of His-tagged Long-R³-IGF-I in a black market product
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Growth Horm IGF Res 20:386-90. 2010
    ..This case report describes the identification of His-tagged Long-R³-IGF-I, which is usually produced for biochemical studies, in an injection vial...
  60. doi request reprint Mass spectrometric detection of siRNA in plasma samples for doping control purposes
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Anal Bioanal Chem 398:1305-12. 2010
    ..25-1 nmol mL(-1)) and performance (precision 11-21%, recovery 23-67%) for typical antisense oligonucleotides currently used in clinical studies...
  61. doi request reprint Comparison of the urinary protein patterns of athletes by 2D-gel electrophoresis and mass spectrometry-a pilot study
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 1:382-6. 2009
    ..Future studies may lead to the establishment of urinary protein maps that are typical for a certain type of sport or even an individual athlete. These maps may complement the blood passport of athletes in doping control...
  62. ncbi request reprint Analysis of confiscated black market drugs using chromatographic and mass spectrometric approaches
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Anal Toxicol 32:232-40. 2008
    ..Except for those drugs supposedly originating from so-called underground labs, fake packings were hardly or not distinguishable from original boxes by visual inspection...
  63. ncbi request reprint Identification of human pituitary growth hormone variants by mass spectrometry
    Maxie Kohler
    Center for Preventive Doping Research, German Sport University Cologne, Germany
    J Proteome Res 8:1071-6. 2009
    ..Besides 22 and 20 kDa isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac 2 NeuAc modification presumably located at Thr 60...
  64. doi request reprint Ultrahigh pressure liquid chromatography-(tandem) mass spectrometry in human sports drug testing: possibilities and limitations
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Chromatogr A 1292:38-50. 2013
    ..In this review, recent applications of UHPLC-MS/MS in sports drug testing procedures published between 2008 and 2012 are presented and advantages as well as limitations in a short- and long-term perspective are discussed...
  65. doi request reprint Does the analysis of the enantiomeric composition of clenbuterol in human urine enable the differentiation of illicit clenbuterol administration from food contamination in sports drug testing?
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Rapid Commun Mass Spectrom 27:507-12. 2013
    ....
  66. doi request reprint Rapid screening of polysaccharide-based plasma volume expanders dextran and hydroxyethyl starch in human urine by liquid chromatography-tandem mass spectrometry
    Sven Guddat
    Institute of Biochemistry, German Sport University, Cologne, Germany
    Biomed Chromatogr 22:695-701. 2008
    ....
  67. doi request reprint Measuring insulin in human vitreous humour using LC-MS/MS
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 4:53-6. 2012
    ..This communication represents the first successful mass-spectrometry-based analysis of post-mortem material related to an insulin poisoning case...