Mario Thevis

Summary

Affiliation: German Sport University
Country: Germany

Publications

  1. doi request reprint Quantification of human insulin-like growth factor-1 and qualitative detection of its analogues in plasma using liquid chromatography/electrospray ionisation tandem mass spectrometry
    Michael Bredehöft
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 22:477-85. 2008
  2. ncbi request reprint Mass spectrometric characterization of efaproxiral (RSR13) and its implementation into doping controls using liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry and the Competence Center for Preventive Anti Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Mass Spectrom 41:332-8. 2006
  3. ncbi request reprint Mass spectrometry in sports drug testing: Structure characterization and analytical assays
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Mass Spectrom Rev 26:79-107. 2007
  4. doi request reprint Determination of 13C/12C ratios of endogenous urinary steroids: method validation, reference population and application to doping control purposes
    Thomas Piper
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Koln, Germany
    Rapid Commun Mass Spectrom 22:2161-75. 2008
  5. doi request reprint Mass spectrometric detection of peginesatide in human urine in doping control analysis
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Pharm Biomed Anal 70:512-7. 2012
  6. doi request reprint Trafficking of drug candidates relevant for sports drug testing: detection of non-approved therapeutics categorized as anabolic and gene doping agents in products distributed via the Internet
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 3:331-6. 2011
  7. doi request reprint Determination of benzimidazole- and bicyclic hydantoin-derived selective androgen receptor antagonists and agonists in human urine using LC-MS/MS
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933, Cologne, Germany
    Anal Bioanal Chem 391:251-61. 2008
  8. ncbi request reprint Unusual mass spectrometric dissociation pathway of protonated isoquinoline-3-carboxamides due to multiple reversible water adduct formation in the gas phase
    Simon Beuck
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    J Am Soc Mass Spectrom 20:2034-48. 2009
  9. doi request reprint Characterization of two major urinary metabolites of the PPARdelta-agonist GW1516 and implementation of the drug in routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2479-91. 2010
  10. doi request reprint Adverse analytical findings with clenbuterol among U-17 soccer players attributed to food contamination issues
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Drug Test Anal 5:372-6. 2013

Detail Information

Publications118 found, 100 shown here

  1. doi request reprint Quantification of human insulin-like growth factor-1 and qualitative detection of its analogues in plasma using liquid chromatography/electrospray ionisation tandem mass spectrometry
    Michael Bredehöft
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 22:477-85. 2008
    ..Four of six samples resulted in concentration ratios with good correlation between both assays, whereas the absolute concentrations were lower for the presented procedure...
  2. ncbi request reprint Mass spectrometric characterization of efaproxiral (RSR13) and its implementation into doping controls using liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry and the Competence Center for Preventive Anti Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Mass Spectrom 41:332-8. 2006
    ..5 ng/ml and interday precisions ranging from 7.9 to 13.0%...
  3. ncbi request reprint Mass spectrometry in sports drug testing: Structure characterization and analytical assays
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Mass Spectrom Rev 26:79-107. 2007
    ....
  4. doi request reprint Determination of 13C/12C ratios of endogenous urinary steroids: method validation, reference population and application to doping control purposes
    Thomas Piper
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Koln, Germany
    Rapid Commun Mass Spectrom 22:2161-75. 2008
    ..In order to determine naturally occurring (13)C/(12)C ratios of all implemented steroids, a reference population of n = 61 subjects was measured to enable the calculation of reference limits for all relevant steroidal Delta values...
  5. doi request reprint Mass spectrometric detection of peginesatide in human urine in doping control analysis
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Pharm Biomed Anal 70:512-7. 2012
    ..v. administration of peginesatide over a period of 4 days...
  6. doi request reprint Trafficking of drug candidates relevant for sports drug testing: detection of non-approved therapeutics categorized as anabolic and gene doping agents in products distributed via the Internet
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 3:331-6. 2011
    ....
  7. doi request reprint Determination of benzimidazole- and bicyclic hydantoin-derived selective androgen receptor antagonists and agonists in human urine using LC-MS/MS
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933, Cologne, Germany
    Anal Bioanal Chem 391:251-61. 2008
    ..4 and 13.2% and between 6.5 and 24.2%, respectively. Recoveries varied from 89 to 106%, and tests for ion suppression or enhancement effects were negative for all analytes. [figure: see text]..
  8. ncbi request reprint Unusual mass spectrometric dissociation pathway of protonated isoquinoline-3-carboxamides due to multiple reversible water adduct formation in the gas phase
    Simon Beuck
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    J Am Soc Mass Spectrom 20:2034-48. 2009
    ....
  9. doi request reprint Characterization of two major urinary metabolites of the PPARdelta-agonist GW1516 and implementation of the drug in routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2479-91. 2010
    ..Validation was performed for specificity, limit of detection (0.1 ng/ml), recovery (72%), intraday and interday precisions (7.7-15.1%), and ion suppression/enhancement effects (<10%)...
  10. doi request reprint Adverse analytical findings with clenbuterol among U-17 soccer players attributed to food contamination issues
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Drug Test Anal 5:372-6. 2013
    ..Hence, governmental contribution is urgently needed to combat the illegal use of clenbuterol in stock breading...
  11. doi request reprint Combination of carbon isotope ratio with hydrogen isotope ratio determinations in sports drug testing
    Thomas Piper
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Koln, Germany
    Anal Bioanal Chem 405:5455-66. 2013
    ..The combination of both stable isotopes would allow for lower reference limits providing the same statistical power and certainty to distinguish between the endo- or exogenous origin of a urinary steroid...
  12. ncbi request reprint Mass spectrometry-based characterization of new drugs and methods of performance manipulation in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 16:301-12. 2010
    ....
  13. doi request reprint Doping control analysis of emerging drugs in human plasma - identification of GW501516, S-107, JTV-519, and S-40503
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:1139-46. 2009
    ..The method was validated with regard to specificity, limits of detection (0.4-8.3 ng/mL), recoveries (72-98%), intraday and interday precisions (12-21%), and ion suppression/enhancement effects...
  14. doi request reprint Mass spectrometric characterization of urinary metabolites of the selective androgen receptor modulator S-22 to identify potential targets for routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Rapid Commun Mass Spectrom 25:2187-95. 2011
    ..The obtained data supports future efforts to effectively screen for and confirm the misuse of the non-approved S-22 drug candidate in doping controls...
  15. ncbi request reprint Quantification of AICAR-ribotide concentrations in red blood cells by means of LC-MS/MS
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933, Cologne, Germany
    Anal Bioanal Chem 405:9703-9. 2013
    ....
  16. ncbi request reprint Effects of gamma irradiation and 15 days of subsequent ex vivo storage on the cytosolic red blood cell proteome analyzed by 2D-DIGE and Orbitrap MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Proteomics Clin Appl 7:561-70. 2013
    ..So far, the effects of ionizing radiation on RBC structure and function and especially the proteome are not fully understood...
  17. doi request reprint Mass spectrometric characterization of urinary metabolites of the selective androgen receptor modulator andarine (S-4) for routine doping control purposes
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 24:2245-54. 2010
    ..The obtained data will support future efforts to effectively screen for and confirm the misuse of the non-approved drug candidate Andarine...
  18. doi request reprint Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry
    Ute Mareck
    Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Drug Test Anal 3:820-7. 2011
    ..Moreover, the excretion of salbutamol glucuronide in human urine following the administration of salbutamol was proven...
  19. ncbi request reprint Screening for 2-quinolinone-derived selective androgen receptor agonists in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 21:3477-86. 2007
    ..01-0.2 ng/mL, and intra- and interday precisions between 3.2 and 8.5% and between 6.3 and 16.6%, respectively. Recoveries varied from 81 to 98%, and tests for ion suppression or enhancement effects were negative for all analytes...
  20. doi request reprint Electron ionization mass spectrometry of the ryanodine receptor-based Ca(2+)-channel stabilizer S-107 and its implementation into routine doping control
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:2363-70. 2009
    ..5-17.2%), as well as recovery (63-66%) were determined. The established procedure proved suitable for routine doping control analysis to detect a potential misuse of the drug candidate S-107 in elite sport...
  21. doi request reprint Doping control analysis of tricyclic tetrahydroquinoline-derived selective androgen receptor modulators using liquid chromatography/electrospray ionization tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 22:2471-8. 2008
    ..The compounds were implemented into existing screening procedures, and detection limits (0.2-0.6 ng/mL), recoveries (92-97%), and intraday and interday precision (<22%) were evaluated...
  22. doi request reprint Characterization of in vitro generated metabolites of the selective androgen receptor modulators S-22 and S-23 and in vivo comparison to post-administration canine urine specimens
    Mario Thevis
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 2:589-98. 2010
    ..4 µg/kg) dosing. Finally, M3 was chemically synthesized, characterized by nuclear magnetic resonance spectroscopy and high resolution/high accuracy mass spectrometry, and chosen as primary target for future doping control analyses...
  23. ncbi request reprint Screening for two selective androgen receptor modulators using gas chromatography-mass spectrometry in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:153-61. 2008
    ....
  24. doi request reprint Temporal indication of cannabis use by means of THC glucuronide determination
    Ute Mareck
    Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 1:505-10. 2009
    ..1 ng/mL), precision (>90%), recovery ( approximately 80%), and extraction efficiency (90%) were determined...
  25. ncbi request reprint Use of an electrochemically synthesised metabolite of a selective androgen receptor modulator for mass spectrometry-based sports drug testing
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:163-70. 2008
    ..The metabolite was included in routine doping control procedures based on liquid chromatography/tandem mass spectrometry and has served as a reference compound for 5000 doping control specimens...
  26. doi request reprint Structure characterisation of urinary metabolites of the cannabimimetic JWH-018 using chemically synthesised reference material for the support of LC-MS/MS-based drug testing
    Simon Beuck
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:493-505. 2011
    ....
  27. doi request reprint Expanding sports drug testing assays: mass spectrometric characterization of the selective androgen receptor modulator drug candidates RAD140 and ACP-105
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Rapid Commun Mass Spectrom 27:1173-82. 2013
    ....
  28. doi request reprint Storage-induced changes of the cytosolic red blood cell proteome analyzed by 2D DIGE and high-resolution/high-accuracy MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Proteomics 12:3263-72. 2012
    ..These can serve as a basis for the development of a screening assay to detect RBC storage lesions and autologous blood doping in sports...
  29. doi request reprint Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Chromatogr A 1259:251-7. 2012
    ..The proof-of-principle was shown by analysing excretion study urine samples for LHRH, Desmopressin and GHRP-2...
  30. doi request reprint Screening for the synthetic cannabinoid JWH-018 and its major metabolites in human doping controls
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 3:609-20. 2011
    ..The method was applied to approximately 7500 urine doping control samples yielding two JWH-018 findings and demonstrated its capability for a sensitive and selective identification of JWH-018 and its metabolites in human urine...
  31. doi request reprint Determination of (13)C/(12)C ratios of endogenous urinary steroids excreted as sulpho conjugates
    Thomas Piper
    German Sport University Cologne, Institute of Biochemistry, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 24:3171-81. 2010
    ....
  32. doi request reprint New drugs and methods of doping and manipulation
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Drug Discov Today 13:59-66. 2008
    ..Although numerous approaches are successful and promising, these methods still have some shortcomings...
  33. doi request reprint Determination of the deuterium/hydrogen ratio of endogenous urinary steroids for doping control purposes
    Thomas Piper
    Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:1917-26. 2009
    ....
  34. doi request reprint Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Drug Test Anal 2:144-8. 2010
    ..Hereby, the content of GHRP-2 was determined with approximately 50 µg per tablet...
  35. doi request reprint Determination of growth hormone releasing peptides (GHRP) and their major metabolites in human urine for doping controls by means of liquid chromatography mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:507-16. 2011
    ..Here, the known metabolite was detectable over 20 h after administration while the intact drug was not observed...
  36. doi request reprint Reporting and managing elevated testosterone/epitestosterone ratios--novel aspects after five years' experience
    Ute Mareck
    Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 2:637-42. 2010
    ..Further conditions triggering IRMS analysis could be suppressed luteinizing hormone (LH) values in males and disproportionate changes of relevant parameters in individual profiles evidently not resulting from ethanol consumption...
  37. ncbi request reprint Analysis of confiscated black market drugs using chromatographic and mass spectrometric approaches
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Anal Toxicol 32:232-40. 2008
    ..Except for those drugs supposedly originating from so-called underground labs, fake packings were hardly or not distinguishable from original boxes by visual inspection...
  38. doi request reprint Annual banned-substance review: the Prohibited List 2008-analytical approaches in human sports drug testing
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, 50933 Cologne, Germany
    Drug Test Anal 1:4-13. 2009
    ..In addition, new techniques in separation and/or ionization of analytes complementary to commonly used procedures were described, which, so far, did not meet all requirements of sports drug testing...
  39. ncbi request reprint Doping-control analysis of the 5alpha-reductase inhibitor finasteride: determination of its influence on urinary steroid profiles and detection of its major urinary metabolite
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    Ther Drug Monit 29:236-47. 2007
    ....
  40. doi request reprint Factors influencing the steroid profile in doping control analysis
    Ute Mareck
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Mass Spectrom 43:877-91. 2008
    ....
  41. doi request reprint Confiscated black market products and nutritional supplements with non-approved ingredients analyzed in the Cologne Doping Control Laboratory 2009
    Maxie Kohler
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany
    Drug Test Anal 2:533-7. 2010
    ..The awareness of new products on the black market and in nutritional supplements is of utmost importance for laboratories to develop detection methods accordingly and screen for new substances as early as possible...
  42. doi request reprint Screening for benfluorex and its major urinary metabolites in routine doping controls
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Anal Bioanal Chem 401:543-51. 2011
    ....
  43. doi request reprint Nutritional supplements cross-contaminated and faked with doping substances
    Hans Geyer
    Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Mass Spectrom 43:892-902. 2008
    ..For the detection of new 'designer' steroids in nutritional supplements, mass spectrometric strategies (GC/MS, LC/MS/MS) are presented...
  44. doi request reprint Traditional Chinese medicine and sports drug testing: identification of natural steroid administration in doping control urine samples resulting from musk (pod) extracts
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Br J Sports Med 47:109-14. 2013
    ....
  45. doi request reprint Detection of peginesatide in equine serum using liquid chromatography-tandem mass spectrometry for doping control purposes
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 18:407-12. 2012
    ....
  46. doi request reprint Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Methods 56:230-5. 2012
    ..is enabled...
  47. ncbi request reprint Identification of human pituitary growth hormone variants by mass spectrometry
    Maxie Kohler
    Center for Preventive Doping Research, German Sport University Cologne, Germany
    J Proteome Res 8:1071-6. 2009
    ..Besides 22 and 20 kDa isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac 2 NeuAc modification presumably located at Thr 60...
  48. ncbi request reprint Determination of selected stimulants in urine for sports drug analysis by solid phase extraction via cation exchange and means of liquid chromatography-tandem mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:135-43. 2008
    ..This method provides a rugged and highly specific alternative to the established method utilising gas or liquid chromatography after liquid-liquid extraction...
  49. ncbi request reprint Doping control analysis of methoxyphenamine using liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:145-52. 2008
    ..7 ng mL(-1)), intraday- and interday precision (2.5-5.8% and 10.8-16.2%, respectively) and its applicability was demonstrated with an authentic doping control sample which tested positive for the prohibited compound early in 2008...
  50. doi request reprint Identification of steroid isoxazole isomers marketed as designer supplement
    Maria K Parr
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Steroids 74:322-8. 2009
    ..They were identified as 17beta-hydroxyandrostano[3,2-c]isoxazole and -[2,3-d]isoxazole...
  51. doi request reprint Emerging drugs: mechanism of action, mass spectrometry and doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    J Mass Spectrom 44:442-60. 2009
    ..Compounds such as ryanodine-calstabin-complex modulators (benzothiazepines) are included, their mass spectrometric properties discussed, and current approaches in sports drug testing outlined...
  52. doi request reprint Annual banned-substance review: analytical approaches in human sports drug testing
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 2:149-61. 2010
    ....
  53. doi request reprint Detection of the arylpropionamide-derived selective androgen receptor modulator (SARM) S-4 (Andarine) in a black-market product
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 1:387-92. 2009
    ..The early inclusion of emerging drugs into routine sports drug testing procedures is a key element of preventive doping research, limiting the options for cheating athletes who aim to undermine the doping control system...
  54. doi request reprint Proteolysis and autolysis of proteases and the detection of degradation products in doping control
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Germany
    Drug Test Anal 1:81-6. 2009
    ..All experiments were performed with fortified urine samples under consideration of assumed realistic conditions for adulteration by cheating sportsmen...
  55. doi request reprint Screening for the calstabin-ryanodine receptor complex stabilizers JTV-519 and S-107 in doping control analysis
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, 50933 Cologne, Germany
    Drug Test Anal 1:32-42. 2009
    ..Validation items such as specificity, recovery (68-92%), lower limit of detection (0.1-0.2 ng/mL), intraday (5.2-18.5%) and interday (8.7-18.8%) precision as well as ion suppression/enhancement effects were determined...
  56. doi request reprint Comprehensive plasma-screening for known and unknown substances in doping controls
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 24:1124-32. 2010
    ..1-10 ng/mL), precision (9-28%), robustness, linearity, ion suppression and recovery (80-112%). In addition to the identification of unknown compounds, the plasma samples were simultaneously screened for known prohibited targets...
  57. doi request reprint Quantification of urinary AICAR concentrations as a matter of doping controls
    Andreas Thomas
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf, 50933 Cologne, Germany
    Anal Bioanal Chem 396:2899-908. 2010
    ..100%), limit of quantification (100 ng/mL), stability and ion suppression effects, employing an in-house synthesised (13)C(5)-labelled AICAR as internal standard...
  58. doi request reprint Stimulants and doping in sport
    Mario Thevis
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany
    Endocrinol Metab Clin North Am 39:89-105, ix. 2010
    ..As a consequence, a reimplementation of this drug in future doping controls was decided...
  59. doi request reprint Doping control analysis of metamfepramone and two major metabolites using liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 15:507-15. 2009
    ..Although the active drug was not detected, the indicative metabolites methylpseudoephedrine and methcathinone were considered sufficient to infer the application of the prohibited drug...
  60. doi request reprint Determination of Synacthen in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 23:2669-74. 2009
    ..For all experiments, the ACTH fragment 1-17 was used as the internal standard...
  61. doi request reprint Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes
    Ines Möller
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 25:2115-23. 2011
    ..Its fitness for purpose was evaluated by the determination of selected method characteristics focusing particularly on specificity, recovery (ca. 60%), and limit of detection (1 ng/mL)...
  62. ncbi request reprint Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Chem 78:1897-903. 2006
    ..A reliable tool is provided that allows the qualitative determination of rapid-acting insulins in urine specimens collected for sports drug testing...
  63. ncbi request reprint Quantitative analysis of urinary glycerol levels for doping control purposes using gas chromatography-mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Eur J Mass Spectrom (Chichester, Eng) 14:117-25. 2008
    ....
  64. ncbi request reprint Mass spectrometric identification and characterization of a new long-term metabolite of metandienone in human urine
    Wilhelm Schanzer
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:2252-8. 2006
    ....
  65. ncbi request reprint Mass spectrometry of hydantoin-derived selective androgen receptor modulators
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    J Mass Spectrom 43:639-50. 2008
    ....
  66. ncbi request reprint Determination of the origin of urinary norandrosterone traces by gas chromatography combustion isotope ratio mass spectrometry
    Moritz Hebestreit
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, Cologne, Germany
    Analyst 131:1021-6. 2006
    ..By comparing delta(13)C(VPDB)-values of androsterone as endogenous reference compound with NA, the origin of NA in doping control samples was determined as either endogenous or exogenous...
  67. doi request reprint Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS
    Katja Walpurgis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Germany
    Electrophoresis 33:2537-45. 2012
    ....
  68. doi request reprint Structure elucidation of the diagnostic product ion at m/z 97 derived from androst-4-en-3-one-based steroids by ESI-CID and IRMPD spectroscopy
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    J Am Soc Mass Spectrom 23:537-46. 2012
    ....
  69. doi request reprint Development of liquid chromatography-tandem mass spectrometry-based analytical assays for the determination of HIF stabilizers in preventive doping research
    Simon Beuck
    Institute of Biochemistry, Centre for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Drug Test Anal 3:756-70. 2011
    ....
  70. doi request reprint Doping control analysis of selected peptide hormones using LC-MS(/MS)
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Forensic Sci Int 213:35-41. 2011
    ....
  71. ncbi request reprint Mass spectrometric identification of degradation products of insulin and its long-acting analogues in human urine for doping control purposes
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Chem 79:2518-24. 2007
    ....
  72. ncbi request reprint Mass spectrometric determination of insulins and their degradation products in sports drug testing
    Mario Thevis
    Institute of Biochemistry and Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, Cologne, Germany
    Mass Spectrom Rev 27:35-50. 2008
    ..Diagnostic product ions and analytical strategies are presented and discussed in light of the need to unambiguously identify misused drugs in urine and plasma specimens for doping control...
  73. ncbi request reprint Determination of Synacthen in human plasma using immunoaffinity purification and liquid chromatography/tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:3551-6. 2006
    ..Authentic plasma samples obtained from a patient undergoing a standard short Synacthen test were used to prove the applicability of the developed procedure...
  74. ncbi request reprint Determination of therapeutics with growth-hormone secretagogue activity in human urine for doping control purposes
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:3393-402. 2006
    ..In both cases, the administered drug and the respective desmethylated metabolites were detected...
  75. ncbi request reprint Identification of the aromatase inhibitors anastrozole and exemestane in human urine using liquid chromatography/tandem mass spectrometry
    Ute Mareck
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:1954-62. 2006
    ..02 ng/mL; exemestane: 3.1 ng/mL; dihydroexemestane: 0.5 ng/mL), interday precisions (6.6-11.1%, 4.9-9.1% and 5.6-8.3% for low [10 ng/mL], medium [50 ng/mL] and high [100 ng/mL] concentration) and recoveries (ranged from 85-97%)...
  76. ncbi request reprint Detection of manipulation in doping control urine sample collection: a multidisciplinary approach to determine identical urine samples
    Mario Thevis
    Center for Preventive Doping Research Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Anal Bioanal Chem 388:1539-43. 2007
    ..Application of a new multidisciplinary strategy employing common and new doping control assays enables the detection of urine substitution in sports drug testing...
  77. ncbi request reprint Metabolism of 4-hydroxyandrostenedione and 4-hydroxytestosterone: Mass spectrometric identification of urinary metabolites
    Maxie Kohler
    Institute of Biochemistry, German Sport University of Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Steroids 72:278-86. 2007
    ..Furthermore 4-hydroxyandrosta-4,6-diene-3,17-dione (29) and 4-hydroxyandrosta-1,4-diene-3,17-dione (30) were determined as oxidation products. Conjugation was diverse and included glucuronidation and sulfatation...
  78. doi request reprint Mass spectrometric determination of gonadotrophin-releasing hormone (GnRH) in human urine for doping control purposes by means of LC-ESI-MS/MS
    Andreas Thomas
    Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50993 Cologne, Germany
    J Mass Spectrom 43:908-15. 2008
    ..5 pg/ml), precision (inter/intraday, < 20%) and accuracy (105%) using Des-pGlu(1)-GnRH as internal standard to control each sample preparation step...
  79. ncbi request reprint Detection of dehydroepiandrosterone misuse by means of gas chromatography- combustion-isotope ratio mass spectrometry
    Ute Mareck
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany koeln de
    Eur J Mass Spectrom (Chichester, Eng) 13:419-26. 2007
    ..These results should be taken into consideration in future discussions about threshold values for endogenous steroids in doping control...
  80. ncbi request reprint Electrospray ionization mass spectrometric characterization and quantitation of xanthine derivatives using isotopically labelled analogues: an application for equine doping control analysis
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, Cologne, Germany
    Rapid Commun Mass Spectrom 18:1553-60. 2004
    ..In addition, the dissociation behavior of deuterated analogues to caffeine and theobromine allowed proposals for fragmentation routes of xanthine derivatives after atmospheric pressure ionization and collisionally activated dissociation...
  81. ncbi request reprint Liquid chromatography/electrospray ionization tandem mass spectrometric screening and confirmation methods for beta2-agonists in human or equine urine
    Mario Thevis
    Institute of Biochemistry, German Sport University, Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Mass Spectrom 38:1197-206. 2003
    ..A single LC/MS/MS analysis can be performed in 9 min...
  82. ncbi request reprint Screening for metabolically stable aryl-propionamide-derived selective androgen receptor modulators for doping control purposes
    Mario Thevis
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 20:870-6. 2006
    ..6-11.6%) and interday precision (9.9-14.4%), and precursor ion scan experiments on diagnostic product ions enabled the detection of a structurally related compound at 50 ng/mL...
  83. ncbi request reprint Identification of oral antidiabetics and their metabolites in human urine by liquid chromatography/tandem mass spectrometry--a matter for doping control analysis
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, Cologne, Germany
    Rapid Commun Mass Spectrom 19:928-36. 2005
    ....
  84. ncbi request reprint Effect of the location of hydrogen abstraction on the fragmentation of diuretics in negative electrospray ionization mass spectrometry
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    J Am Soc Mass Spectrom 14:658-70. 2003
    ..Moreover, the generation of radical anions by collision-activated dissociation of even-electron precursor ions was observed, the generation of which is discussed by proposed fragmentation pathways...
  85. doi request reprint Determination of IGF-1 and IGF-2, their degradation products and synthetic analogues in urine by LC-MS/MS
    Andreas Thomas
    Institute of Biochemistry Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany
    Analyst 136:1003-12. 2011
    ..The method was validated for qualitative purposes considering the parameters specificity, limit of detection (20-50 pg mL(-1)), recovery (10-35%), precision (<20%), linearity, robustness and stability...
  86. doi request reprint Annual banned-substance review: analytical approaches in human sports drug testing
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Germany
    Drug Test Anal 3:1-14. 2011
    ..This review surveys new and/or enhanced procedures and techniques of doping analysis together with information relevant to doping control that has been published in the literature between October 2009 and September 2010...
  87. ncbi request reprint Mass spectrometry of stanozolol and its analogues using electrospray ionization and collision-induced dissociation with quadrupole-linear ion trap and linear ion trap-orbitrap hybrid mass analyzers
    Mario Thevis
    Institute of Biochemistry and the Competence Center for Preventive Anti Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Rapid Commun Mass Spectrom 19:3369-78. 2005
    ..In addition, a charge-remote fragmentation causing the neutral loss of methanol was observed, which was suggested to be composed by the methyl residue at C-18 and the hydroxyl function located at C-17...
  88. ncbi request reprint N-methyl-N-trimethylsilyltrifluoroacetamide-promoted synthesis and mass spectrometric characterization of deuterated ephedrines
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany koeln de
    Eur J Mass Spectrom (Chichester, Eng) 10:673-81. 2004
    ....
  89. ncbi request reprint Characterization of chemically modified steroids for doping control purposes by electrospray ionization tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Mass Spectrom 40:494-502. 2005
    ....
  90. ncbi request reprint Mass spectrometric analysis of androstan-17beta-ol-3-one and androstadiene-17beta-ol-3-one isomers
    Mario Thevis
    Institute of Biochemistry, German Sport University of Cologne, Cologne, Germany
    J Am Soc Mass Spectrom 16:1660-9. 2005
    ....
  91. ncbi request reprint Identification and quantification of the plasma volume expander dextran in human urine by liquid chromatography-tandem mass spectrometry of enzymatically derived isomaltose
    Sven Guddat
    Institute of Biochemistry, German Sport University, Cologne, Germany
    Biomed Chromatogr 19:743-50. 2005
    ....
  92. ncbi request reprint Examples of doping control analysis by liquid chromatography-tandem mass spectrometry: ephedrines, beta-receptor blocking agents, diuretics, sympathomimetics, and cross-linked hemoglobins
    Mario Thevis
    Institute of Biochemistry, German Sport University, Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Chromatogr Sci 43:22-31. 2005
    ....
  93. ncbi request reprint Screening for unknown synthetic steroids in human urine by liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933, Cologne, Germany
    J Mass Spectrom 40:955-62. 2005
    ..Subsequent product ion scan experiments on precursor ions of peaks of unknown origin provided structural identification of the unknown compounds...
  94. ncbi request reprint Quantitative determination of metabolic products of 19-norandrostenediol in human plasma using gas chromatography/mass spectrometry
    Yvonne Schrader
    Institute of Biochemistry, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Drug Metab Dispos 34:1328-35. 2006
    ..3 to 1.2 ng/ml have been reported to influence endocrinological parameters...
  95. doi request reprint Determination of the prevalence of anabolic steroids, stimulants, and selected drugs subject to doping controls among elite sport students using analytical chemistry
    Mario Thevis
    Centre for Preventive Doping Research, Institute of Biochemistry, German Sport University, Cologne, Germany
    J Sports Sci 26:1059-65. 2008
    ..However, evidence for stimulants and tetrahydrocannabinol administration was obtained, although not reported by any participant, which highlights the issue of under-reporting in surveys based solely on questionnaires...
  96. doi request reprint Doping control analysis of trenbolone and related compounds using liquid chromatography-tandem mass spectrometry
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany
    Steroids 74:315-21. 2009
    ..3-3ng/mL), recovery (72-105%), intraday and interday precision (< or =20%)...
  97. doi request reprint Mass spectrometry of selective androgen receptor modulators
    Mario Thevis
    Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    J Mass Spectrom 43:865-76. 2008
    ..The complexity and structural heterogeneity of these drugs is a daunting challenge for detection methods...
  98. doi request reprint [Doping control activities in Germany 1989-2006]
    Mario Thevis
    Zentrum für Präventive Dopingforschung Institut für Biochemie, Deutsche Sporthochschule Koln, Carl Diem Weg 6, 50933 Koln
    Med Klin (Munich) 103:282-98. 2008
    ..In light of recent doping scandals and confessions in 2007, a critical review of national antidoping actions is considered necessary...
  99. ncbi request reprint Aryl-propionamide-derived selective androgen receptor modulators: liquid chromatography-tandem mass spectrometry characterization of the in vitro synthesized metabolites for doping control purposes
    Tiia Kuuranne
    Institute of Biochemistry, Center for Preventive Doping Research, German Sports University Cologne, Carl Diem Weg 6, 50933 Cologne, Germany
    Drug Metab Dispos 36:571-81. 2008
    ..These structure-specific ions may be monitored as target ions in the routine doping control...
  100. ncbi request reprint Gas phase reaction of substituted isoquinolines to carboxylic acids in ion trap and triple quadrupole mass spectrometers after electrospray ionization and collision-induced dissociation
    Mario Thevis
    Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany
    J Am Soc Mass Spectrom 19:151-8. 2008
    ....
  101. doi request reprint Rapid screening of polysaccharide-based plasma volume expanders dextran and hydroxyethyl starch in human urine by liquid chromatography-tandem mass spectrometry
    Sven Guddat
    Institute of Biochemistry, German Sport University, Cologne, Germany
    Biomed Chromatogr 22:695-701. 2008
    ....