Research Topics
Genomes and Genes | Georg StoecklinSummaryAffiliation: German Cancer Research Center Country: Germany Publications
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Publications
MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decayGeorg Stoecklin
Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
EMBO J 23:1313-24. 2004..Our data reveal the mechanism by which the p38-MAPK/MK2 kinase cascade inhibits TTP-mediated degradation of ARE-containing transcripts and thereby contributes to lipopolysaccharide-induced TNFalpha expression...
Relationship of GW/P-bodies with stress granulesGeorg Stoecklin
German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
Adv Exp Med Biol 768:197-211. 2013..Currently available data suggest that these self-assembling compartments are controlled by flux of mRNAs within the cytoplasm, and that their assembly mirrors the translation and degradation rates of their component mRNAs...- Genome-wide analysis identifies interleukin-10 mRNA as target of tristetraprolinGeorg Stoecklin
Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 283:11689-99. 2008..IL-10 mRNA levels were found to be elevated because of a reduced decay rate in primary macrophages from TTP(-/-) mice. Our study demonstrates the importance of experimental approaches for identifying targets of RNA-binding proteins... - Stress granules and processing bodies are dynamically linked sites of mRNP remodelingNancy Kedersha
Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
J Cell Biol 169:871-84. 2005..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation... - Translation suppression promotes stress granule formation and cell survival in response to cold shockSarah Hofmann
Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, Center for Organismal Studies, Heidelberg, Germany
Mol Biol Cell 23:3786-800. 2012..Our results demonstrate that cells actively suppress protein synthesis by parallel pathways, which induce SG formation and ensure cellular survival during hypothermia... 
T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAsSatoshi Yamasaki
Harvard Medical School, Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 282:30070-7. 2007..These results suggest that TIA-1-induced polysome disassembly is required for enhanced mRNA decay and that TIA-1-induced translational silencing promotes the decay of selected mRNAs...- Stress granule assembly is mediated by prion-like aggregation of TIA-1Natalie Gilks
Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
Mol Biol Cell 15:5383-98. 2004..Our results reveal that prion-like aggregation of TIA-1 regulates SG formation downstream of eIF2alpha phosphorylation in response to stress... - Genome-wide assessment of AU-rich elements by the AREScore algorithmMilan Spasic
Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
PLoS Genet 8:e1002433. 2012..Taken together, this study introduces AREScore as a simple tool to identify ARE-containing mRNAs and provides compelling evidence that AREs are widespread regulatory elements in Drosophila... - Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodiesSevim Ozgur
German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
Mol Cell Biol 30:4308-23. 2010..Taken together, these findings provide evidence that human Pat1b is a central component of the RNA decay machinery by physically connecting deadenylation with decapping... - On track with P-bodiesMeeta Kulkarni
Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Biochem Soc Trans 38:242-51. 2010..In the present review, we summarize recent findings on the function, assembly and motility of P-bodies. An updated list of proteins and RNAs that localize to P-bodies will help in keeping track of this fast-growing field... - ARE-mRNA degradation requires the 5'-3' decay pathwayGeorg Stoecklin
Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith 608, Boston, Massachusetts 02115, USA
EMBO Rep 7:72-7. 2006..On the other side, out of three exosome components tested, only knockdown of PmScl-75 caused a strong inhibition of AMD. Our results show that mammalian cells, similar to yeast, require the 5'-3' Xrn1 pathway to degrade ARE-mRNAs... - Control of mRNA decay by phosphorylation of tristetraprolinHeike Sandler
Helmholtz Junior Research Group Post transcriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Biochem Soc Trans 36:491-6. 2008..In the present review, we address the target specificity of TTP, summarize TTP-interacting proteins and discuss how phosphorylation regulates the activity, localization and stability of TTP... - Post-transcriptional regulation of proinflammatory proteinsPaul Anderson
Immunology and Allergy, Brigham and Women s Hospital, Smith 652, One Jimmy Fund Way, Boston, MA 02115, USA
J Leukoc Biol 76:42-7. 2004..We hypothesize that TIA-1-/-TTP-/- neutrophils are a source of arthritigenic TNF-alpha, which promotes severe erosive arthritis in these mice... - In a tight spot: ARE-mRNAs at processing bodiesGeorg Stoecklin
German Cancer Research Center, 69120 Heidelberg, Germany
Genes Dev 21:627-31. 2007 - RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesisFrancesca Tuorto
Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Nat Struct Mol Biol 19:900-5. 2012..These results establish a biologically important function for cytosine-C5 tRNA methylation in mammals and suggest that this modification promotes mouse development by supporting protein synthesis... - Protein synthesis and translational control: at eye level with the ribosomeKathrin Leppek
Helmholtz Junior Research Group, Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg 69120, Germany
EMBO Rep 12:1214-6. 2011.... - Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolinHeike Sandler
Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Nucleic Acids Res 39:4373-86. 2011..Thus, cytoplasmic Not1 provides a platform that allows a specific RNA binding protein to recruit the Caf1 deadenylase and thereby trigger decay of its target mRNAs... - Networks controlling mRNA decay in the immune systemJohanna Schott
Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Wiley Interdiscip Rev RNA 1:432-56. 2010.... - Tristetraprolin (TTP)-14-3-3 complex formation protects TTP from dephosphorylation by protein phosphatase 2a and stabilizes tumor necrosis factor-alpha mRNALei Sun
Division of Critical Care, Department of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
J Biol Chem 282:3766-77. 2007..Our data indicate that, although phosphorylation stabilizes TNF-alpha mRNA, PP2A regulates the mRNA stability by modulating the phosphorylation state of members of the p38/MK-2/TTP pathway... - A GFP-based assay for monitoring post-transcriptional regulation of ARE-mRNA turnoverDon Benjamin
Institute for Medical Microbiology, Petersplatz 10, 4003 Basel, Switzerland
Mol Biosyst 2:561-7. 2006..The simplicity and effectiveness of this screen makes it ideal for screening of modulators of clinically important ARE-bearing transcripts such as TNFalpha, VEGF, the interferons and other cytokines... - The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase BMartin Schmidlin
Institute for Medical Microbiology, University of Basel, Basel, Switzerland
EMBO J 23:4760-9. 2004..In vivo and in vitro data support a model where PKB causes ARE-mRNA stabilization by inactivating BRF1 through binding to 14-3-3... - A constitutive decay element promotes tumor necrosis factor alpha mRNA degradation via an AU-rich element-independent pathwayGeorg Stoecklin
Institute of Medical Microbiology, University of Basel, Petersplatz 10, CH-4003 Basel, Switzerland
Mol Cell Biol 23:3506-15. 2003..Thus, CDE-mediated mRNA decay is likely to be an important mechanism limiting LPS-induced pathologic processes... - A novel mechanism of tumor suppression by destabilizing AU-rich growth factor mRNAGeorg Stoecklin
Institute of Medical Microbiology, University of Basel, Petersplatz 10, CH-4003 Basel, Switzerland
Oncogene 22:3554-61. 2003.... - Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnoverGeorg Stoecklin
Institute of Medical Microbiology, University of Basel, Petersplatz 10, 4003 Basel, Switzerland
EMBO J 21:4709-18. 2002..This approach, which identified BRF1 as an essential regulator of ARE-dependent mRNA decay, should also be applicable to other cis-elements of mRNA turnover...