Georg Stoecklin

Summary

Affiliation: German Cancer Research Center
Country: Germany

Publications

  1. pmc MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 23:1313-24. 2004
  2. doi request reprint Relationship of GW/P-bodies with stress granules
    Georg Stoecklin
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Adv Exp Med Biol 768:197-211. 2013
  3. pmc Genome-wide analysis identifies interleukin-10 mRNA as target of tristetraprolin
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:11689-99. 2008
  4. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
  5. pmc Translation suppression promotes stress granule formation and cell survival in response to cold shock
    Sarah Hofmann
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, Center for Organismal Studies, Heidelberg, Germany
    Mol Biol Cell 23:3786-800. 2012
  6. ncbi request reprint T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAs
    Satoshi Yamasaki
    Harvard Medical School, Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 282:30070-7. 2007
  7. pmc Stress granule assembly is mediated by prion-like aggregation of TIA-1
    Natalie Gilks
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Biol Cell 15:5383-98. 2004
  8. pmc Genome-wide assessment of AU-rich elements by the AREScore algorithm
    Milan Spasic
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    PLoS Genet 8:e1002433. 2012
  9. pmc Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies
    Sevim Ozgur
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Mol Cell Biol 30:4308-23. 2010
  10. pmc Role of Rck-Pat1b binding in assembly of processing-bodies
    Sevim Ozgur
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center DKFZ ZMBH Alliance, Heidelberg, Germany
    RNA Biol 10:528-39. 2013

Collaborators

Detail Information

Publications27

  1. pmc MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 23:1313-24. 2004
    ..Our data reveal the mechanism by which the p38-MAPK/MK2 kinase cascade inhibits TTP-mediated degradation of ARE-containing transcripts and thereby contributes to lipopolysaccharide-induced TNFalpha expression...
  2. doi request reprint Relationship of GW/P-bodies with stress granules
    Georg Stoecklin
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Adv Exp Med Biol 768:197-211. 2013
    ..Currently available data suggest that these self-assembling compartments are controlled by flux of mRNAs within the cytoplasm, and that their assembly mirrors the translation and degradation rates of their component mRNAs...
  3. pmc Genome-wide analysis identifies interleukin-10 mRNA as target of tristetraprolin
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:11689-99. 2008
    ..IL-10 mRNA levels were found to be elevated because of a reduced decay rate in primary macrophages from TTP(-/-) mice. Our study demonstrates the importance of experimental approaches for identifying targets of RNA-binding proteins...
  4. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  5. pmc Translation suppression promotes stress granule formation and cell survival in response to cold shock
    Sarah Hofmann
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, Center for Organismal Studies, Heidelberg, Germany
    Mol Biol Cell 23:3786-800. 2012
    ..Our results demonstrate that cells actively suppress protein synthesis by parallel pathways, which induce SG formation and ensure cellular survival during hypothermia...
  6. ncbi request reprint T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAs
    Satoshi Yamasaki
    Harvard Medical School, Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 282:30070-7. 2007
    ..These results suggest that TIA-1-induced polysome disassembly is required for enhanced mRNA decay and that TIA-1-induced translational silencing promotes the decay of selected mRNAs...
  7. pmc Stress granule assembly is mediated by prion-like aggregation of TIA-1
    Natalie Gilks
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Biol Cell 15:5383-98. 2004
    ..Our results reveal that prion-like aggregation of TIA-1 regulates SG formation downstream of eIF2alpha phosphorylation in response to stress...
  8. pmc Genome-wide assessment of AU-rich elements by the AREScore algorithm
    Milan Spasic
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    PLoS Genet 8:e1002433. 2012
    ..Taken together, this study introduces AREScore as a simple tool to identify ARE-containing mRNAs and provides compelling evidence that AREs are widespread regulatory elements in Drosophila...
  9. pmc Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies
    Sevim Ozgur
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Mol Cell Biol 30:4308-23. 2010
    ..Taken together, these findings provide evidence that human Pat1b is a central component of the RNA decay machinery by physically connecting deadenylation with decapping...
  10. pmc Role of Rck-Pat1b binding in assembly of processing-bodies
    Sevim Ozgur
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center DKFZ ZMBH Alliance, Heidelberg, Germany
    RNA Biol 10:528-39. 2013
    ..Our data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping...
  11. doi request reprint On track with P-bodies
    Meeta Kulkarni
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Biochem Soc Trans 38:242-51. 2010
    ..In the present review, we summarize recent findings on the function, assembly and motility of P-bodies. An updated list of proteins and RNAs that localize to P-bodies will help in keeping track of this fast-growing field...
  12. doi request reprint Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs
    Kathrin Leppek
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Cell 153:869-81. 2013
    ..Thus, Roquin proteins act broadly as mediators of mRNA deadenylation by recognizing a conserved class of stem-loop RNA degradation motifs...
  13. pmc ARE-mRNA degradation requires the 5'-3' decay pathway
    Georg Stoecklin
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith 608, Boston, Massachusetts 02115, USA
    EMBO Rep 7:72-7. 2006
    ..On the other side, out of three exosome components tested, only knockdown of PmScl-75 caused a strong inhibition of AMD. Our results show that mammalian cells, similar to yeast, require the 5'-3' Xrn1 pathway to degrade ARE-mRNAs...
  14. pmc Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin
    Heike Sandler
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nucleic Acids Res 39:4373-86. 2011
    ..Thus, cytoplasmic Not1 provides a platform that allows a specific RNA binding protein to recruit the Caf1 deadenylase and thereby trigger decay of its target mRNAs...
  15. doi request reprint Control of mRNA decay by phosphorylation of tristetraprolin
    Heike Sandler
    Helmholtz Junior Research Group Post transcriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Biochem Soc Trans 36:491-6. 2008
    ..In the present review, we address the target specificity of TTP, summarize TTP-interacting proteins and discuss how phosphorylation regulates the activity, localization and stability of TTP...
  16. ncbi request reprint Post-transcriptional regulation of proinflammatory proteins
    Paul Anderson
    Immunology and Allergy, Brigham and Women s Hospital, Smith 652, One Jimmy Fund Way, Boston, MA 02115, USA
    J Leukoc Biol 76:42-7. 2004
    ..We hypothesize that TIA-1-/-TTP-/- neutrophils are a source of arthritigenic TNF-alpha, which promotes severe erosive arthritis in these mice...
  17. ncbi request reprint In a tight spot: ARE-mRNAs at processing bodies
    Georg Stoecklin
    German Cancer Research Center, 69120 Heidelberg, Germany
    Genes Dev 21:627-31. 2007
  18. doi request reprint RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis
    Francesca Tuorto
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nat Struct Mol Biol 19:900-5. 2012
    ..These results establish a biologically important function for cytosine-C5 tRNA methylation in mammals and suggest that this modification promotes mouse development by supporting protein synthesis...
  19. pmc Protein synthesis and translational control: at eye level with the ribosome
    Kathrin Leppek
    Helmholtz Junior Research Group, Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg 69120, Germany
    EMBO Rep 12:1214-6. 2011
    ....
  20. pmc Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs
    Valentin Färber
    DKFZ ZMBH Alliance, Zentrum fur Molekulare Biologie der Universitat Heidelberg, Im Neuenheimer Feld 282, D69120 Heidelberg, Germany
    Nucleic Acids Res 41:1211-22. 2013
    ..The results further suggest that TbCAF1 is only able to deadenylate mRNA in vivo if it is recruited to the mRNA through other NOT complex components...
  21. doi request reprint Networks controlling mRNA decay in the immune system
    Johanna Schott
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, German Cancer Research Center, DKFZ ZMBH Alliance, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Wiley Interdiscip Rev RNA 1:432-56. 2010
    ....
  22. pmc The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B
    Martin Schmidlin
    Institute for Medical Microbiology, University of Basel, Basel, Switzerland
    EMBO J 23:4760-9. 2004
    ..In vivo and in vitro data support a model where PKB causes ARE-mRNA stabilization by inactivating BRF1 through binding to 14-3-3...
  23. ncbi request reprint Tristetraprolin (TTP)-14-3-3 complex formation protects TTP from dephosphorylation by protein phosphatase 2a and stabilizes tumor necrosis factor-alpha mRNA
    Lei Sun
    Division of Critical Care, Department of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 282:3766-77. 2007
    ..Our data indicate that, although phosphorylation stabilizes TNF-alpha mRNA, PP2A regulates the mRNA stability by modulating the phosphorylation state of members of the p38/MK-2/TTP pathway...
  24. ncbi request reprint A GFP-based assay for monitoring post-transcriptional regulation of ARE-mRNA turnover
    Don Benjamin
    Institute for Medical Microbiology, Petersplatz 10, 4003 Basel, Switzerland
    Mol Biosyst 2:561-7. 2006
    ..The simplicity and effectiveness of this screen makes it ideal for screening of modulators of clinically important ARE-bearing transcripts such as TNFalpha, VEGF, the interferons and other cytokines...
  25. ncbi request reprint A novel mechanism of tumor suppression by destabilizing AU-rich growth factor mRNA
    Georg Stoecklin
    Institute of Medical Microbiology, University of Basel, Petersplatz 10, CH 4003 Basel, Switzerland
    Oncogene 22:3554-61. 2003
    ....
  26. pmc A constitutive decay element promotes tumor necrosis factor alpha mRNA degradation via an AU-rich element-independent pathway
    Georg Stoecklin
    Institute of Medical Microbiology, University of Basel, Petersplatz 10, CH 4003 Basel, Switzerland
    Mol Cell Biol 23:3506-15. 2003
    ..Thus, CDE-mediated mRNA decay is likely to be an important mechanism limiting LPS-induced pathologic processes...
  27. pmc Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover
    Georg Stoecklin
    Institute of Medical Microbiology, University of Basel, Petersplatz 10, 4003 Basel, Switzerland
    EMBO J 21:4709-18. 2002
    ..This approach, which identified BRF1 as an essential regulator of ARE-dependent mRNA decay, should also be applicable to other cis-elements of mRNA turnover...