Research Topics
Genomes and Genes | C NiehrsSummaryAffiliation: German Cancer Research Center Country: Germany Publications
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Detail Information
Publications
Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signallingBingyu Mao
Molecular Embryology Division, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nature 417:664-7. 2002..The results indicate that Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signalling through LRP6 in vertebrates...- Axis formation: redundancy rulesChristof Niehrs
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Curr Biol 15:R391-3. 2005..The role of BMP antagonists in the Spemann-Mangold organizer of vertebrate embryos is a controversial issue. A study using combined knock down of multiple antagonists finally reveals dramatic effects... - Regionally specific induction by the Spemann-Mangold organizerChristof Niehrs
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Nat Rev Genet 5:425-34. 2004 
On growth and form: a Cartesian coordinate system of Wnt and BMP signaling specifies bilaterian body axesChristof Niehrs
Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Development 137:845-57. 2010....- Dialectics, systems biology and embryonic inductionChristof Niehrs
Division of Molecular Embryology, DKFZ ZMBH Alliance, German Cancer Research Center, Im Neuenheimer Feld 581, D 69120 Heidelberg, Germany
Differentiation 81:209-16. 2011..The example highlights that despite its shortcomings, dialectics can be of heuristic value as a theory of systems biology... - Function and biological roles of the Dickkopf family of Wnt modulatorsC Niehrs
Department of Molecular Embryology, German Cancer Research Center, Heidelberg, Germany
Oncogene 25:7469-81. 2006..In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disease... - Active DNA demethylation and DNA repairChristof Niehrs
Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 581, D 69120 Heidelberg, Germany
Differentiation 77:1-11. 2009..Far from merely protecting genomic integrity from environmental insult, DNA repair is therefore at the heart of an epigenetic activation process... 
The zinc finger gene Xblimp1 controls anterior endomesodermal cell fate in Spemann's organizerF S de Souza
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
EMBO J 18:6062-72. 1999..Our results indicate that Xblimp1 is required for anterior endomesodermal cell fate and head induction...- LDL-receptor-related protein 6 is a receptor for Dickkopf proteinsB Mao
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Nature 411:321-5. 2001..Thus, DKKs inhibit Wnt co-receptor function, exemplifying the modulation of LRP signalling by antagonists... - Phenotypic effects in Xenopus and zebrafish suggest that one-eyed pinhead functions as antagonist of BMP signallingC Kiecker
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Mech Dev 94:37-46. 2000.... - A morphogen gradient of Wnt/beta-catenin signalling regulates anteroposterior neural patterning in XenopusC Kiecker
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Development 128:4189-201. 2001..Our results indicate that an activity gradient of Wnt/beta-catenin signalling acts as transforming morphogen to pattern the Xenopus central nervous system... - Dickkopf1 and the Spemann-Mangold head organizerC Niehrs
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Int J Dev Biol 45:237-40. 2001..Injection of inhibitory antibodies leads to microcephaly and cyclopia. Dkk1 thus is an essential mediator of the vertebrate head organizer... - Silencing of TGF-beta signalling by the pseudoreceptor BAMBID Onichtchouk
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Nature 401:480-5. 1999..The results indicate that BAMBI negatively regulates TGF-beta-family signalling by a regulatory mechanism involving the interaction of signalling receptors with a pseudoreceptor... - The Xvent-2 homeobox gene is part of the BMP-4 signalling pathway controlling [correction of controling] dorsoventral patterning of Xenopus mesodermD Onichtchouk
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Development 122:3045-53. 1996..5) Microinjection of Xvent-2 mRNA can rescue dorsalization by a dominant-negative BMP-4 receptor. The results suggest that Xvent-2 functions in the BMP-4 signalling pathway that antagonizes the Spemann organizer... - Mutual antagonism between dickkopf1 and dickkopf2 regulates Wnt/beta-catenin signallingW Wu
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Curr Biol 10:1611-4. 2000..The study identifies Dkk2 as a secreted molecule that is able to activate Wnt/beta-catenin signalling. The results suggest that a coordinated interplay between inhibiting dkk1 and activating dkk2 can modulate Fz signalling... - Dickkopf-1 is a member of a new family of secreted proteins and functions in head inductionA Glinka
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Nature 391:357-62. 1998..Injections of mRNA and antibody indicate that dkk-1 is sufficient and necessary to cause head induction. dkk-1 s a potent antagonist of Wnt signalling, suggesting that dkk genes encode a family of secreted Wnt inhibitors... - Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterningV Gawantka
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120, Heidelberg, Germany
Mech Dev 77:95-141. 1998..In conclusion, this study describes a semi-functional approach to investigate genes expressed during early development and provides global insight into embryonic patterning... - Dickkopf genes are co-ordinately expressed in mesodermal lineagesA P Monaghan
Division of Molecular Biology of the Cell I, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120, Heidelberg, Germany
Mech Dev 87:45-56. 1999..Our observations indicate that dkk genes constitute a new family of secreted proteins that may mediate inductive interactions between epithelial and mesenchymal cells... - Combinatorial signalling by Xwnt-11 and Xnr3 in the organizer epitheliumA Glinka
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Mech Dev 60:221-31. 1996..The results indicate that induction by the organizer epithelium may result from the combinatorial action of instructive Xnr3 and permissive Xwnt-11 signalling... - Axeldb: a Xenopus laevis database focusing on gene expressionN Pollet
Department of Molecular Embryology, Deutsches Krebsforschungszentrum, lm Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nucleic Acids Res 28:139-40. 2000..Axeldb is implemented using an ACEDB database system, and available through the web at http://www.dkfz-heidelberg.de/abt0135/axeldb.htm.. - Kremen2 modulates Dickkopf2 activity during Wnt/LRP6 signalingBingyu Mao
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120, Heidelberg, Germany
Gene 302:179-83. 2003..Finally, we show that interaction with Krm2 is mediated by the second cysteine-rich domain of Dkks. These results suggest that Krm2 can function as a switch that turns Dkk2 from an activator into an inhibitor of Wnt/lRP6 signaling... - Requirement of prorenin receptor and vacuolar H+-ATPase-mediated acidification for Wnt signalingCristina Maria Cruciat
Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Science 327:459-63. 2010..The results reveal an unsuspected role for the prorenin receptor, V-ATPase activity, and acidification during Wnt/beta-catenin signaling... - Kremen proteins interact with Dickkopf1 to regulate anteroposterior CNS patterningGary Davidson
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Development 129:5587-96. 2002..The results indicate that Krm proteins function in a Wnt inhibition pathway regulating early AP patterning of the CNS... - RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicaseP Askjaer
Department of Gene Expression, Deutsches Krebsforschungszentrum, Heidelberg, Germany
Mol Cell Biol 19:6276-85. 1999..In addition, we identify the Xenopus homologue of the nucleoporin CAN/Nup214 as a RanGTP- and NES cargo-specific binding site for CRM1, suggesting that this nucleoporin plays a role in export complex disassembly and/or CRM1 recycling... - Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transductionGary Davidson
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nature 438:867-72. 2005..Our results reveal an evolutionarily conserved mechanism that couples Wnt receptor activation to the cytoplasmic signal transduction apparatus... - Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylationJosipa Bilic
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Science 316:1619-22. 2007..We propose that Wnts induce coclustering of receptors and Dvl in LRP6-signalosomes, which in turn triggers LRP6 phosphorylation to promote Axin recruitment and beta-catenin stabilization... - Unc5B interacts with FLRT3 and Rnd1 to modulate cell adhesion in Xenopus embryosEmil Karaulanov
Division of Molecular Embryology, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
PLoS ONE 4:e5742. 2009..The results suggest that FLRT3, Unc5B and Rnd1 proteins interact to modulate cell adhesion in early Xenopus development... - Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylationGuillermo Barreto
Division of Molecular Embryology, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nature 445:671-5. 2007..Active demethylation occurs by DNA repair and Gadd45a interacts with and requires the DNA repair endonuclease XPG. We conclude that Gadd45a relieves epigenetic gene silencing by promoting DNA repair, which erases methylation marks... - Transcriptional regulation of BMP4 synexpression in transgenic XenopusEmil Karaulanov
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, Germany
EMBO J 23:844-56. 2004..The results highlight that in vivo analysis is required to reveal the physiological, spatio-temporal regulation of BMP-responsive genes... - An atlas of differential gene expression during early Xenopus embryogenesisNicolas Pollet
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Mech Dev 122:365-439. 2005..In conclusion, this study represents an atlas of gene expression patterns, which reveals embryonic regionalization, provides novel marker genes, and makes predictions about the functional role of unknown genes... - The MRH protein Erlectin is a member of the endoplasmic reticulum synexpression group and functions in N-glycan recognitionCristina Maria Cruciat
Department of Molecular Embryology, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
J Biol Chem 281:12986-93. 2006..The results indicate that Erlectin functions in N-glycan recognition in the endoplasmic reticulum, suggesting that it may regulate glycoprotein traffic... - Kremen is required for neural crest induction in Xenopus and promotes LRP6-mediated Wnt signalingChristine Hassler
Department of Molecular Embryology, German Cancer Research Center, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
Development 134:4255-63. 2007..Furthermore, Krm2 knockdown specifically reduces LRP6 protein levels in NC explants. The results indicate that in the absence of Dkks, Kremens activate Wnt/beta-catenin signaling through LRP6... - Dkk3 is required for TGF-beta signaling during Xenopus mesoderm inductionSonia Pinho
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
Differentiation 75:957-67. 2007..Furthermore, depletion of Dkk3 up-regulates FGF signaling, while Dkk3 overexpression reduces it. These results indicate that Dkk3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development... - An ancient Wnt-Dickkopf antagonism in HydraCorina Guder
Molecular Evolution and Genomics, Heidelberg University, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
Development 133:901-11. 2006..Based on the corresponding expression patterns of hydkk1/2/4 and neuronal genes, we suggest that the body column of Hydra is a neurogenic environment suppressing Wnt signalling and facilitating neurogenesis... - The transmembrane protein XFLRT3 forms a complex with FGF receptors and promotes FGF signallingRalph T Böttcher
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Nat Cell Biol 6:38-44. 2004..The results indicate that XFLRT3 is a transmembrane modulator of FGF-MAP kinase signalling in vertebrates... - Norrin and frizzled; a new vein for the eyeChristof Niehrs
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Dev Cell 6:453-4. 2004..Though we become accustomed to novel components, the discovery of an unexpected Wnt receptor ligand, Norrin, is surprising even to the connoisseur... - Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1V Gawantka
Division of Molecular Embryology, German Cancer Research Center, Heidelberg, Germany
EMBO J 14:6268-79. 1995..Third, microinjection of Xvent-1 mRNA ventralizes dorsal mesoderm. The results suggest that Xvent-1 functions in a ventral signaling pathway that maintains the ventral mesodermal state and antagonizes the Spemann organizer... - Regulation of Lrp6 phosphorylationChristof Niehrs
Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 581, Heidelberg, Germany
Cell Mol Life Sci 67:2551-62. 2010.... - Cyclic expression of esr9 gene in Xenopus presomitic mesodermYan Li
Division of Molecular Embryology, Deutches Krebsforschungszentrum, Heidelberg, Germany
Differentiation 71:83-9. 2003..Thus, expression of esr9 and esr10 is an indicator of the segmentation clock in the amphibian embryo. This confirms the evolutionary conservation of a molecular pathway involved in vertebrate segmentation clock... - Wnt/Frizzled signaling requires dPRR, the Drosophila homolog of the prorenin receptorTina Buechling
Division of Signaling and Functional Genomics, German Cancer Research Center DKFZ, and Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, University of Heidelberg, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
Curr Biol 20:1263-8. 2010..Furthermore, dPRR binds to Fz and Fz2 receptors. In summary, our data suggest that dPRR has an evolutionarily conserved role at the receptor level for activation of canonical and noncanonical Wnt/Fz signaling pathways... - Dkk1 and noggin cooperate in mammalian head inductionIvan del Barco Barrantes
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Genes Dev 17:2239-44. 2003..The results provide genetic evidence for the dual inhibition model and indicate that dkk1 and noggin functionally cooperate in the head organizer... - R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling and is required for Xenopus myogenesisOlga Kazanskaya
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Dev Cell 7:525-34. 2004..In Xenopus embryos depleted of Rspo2, the muscle markers myoD and myf5 fail to be activated and later muscle development is impaired. Thus, Rspo2 functions in a positive feedback loop to stimulate the Wnt/beta-catenin cascade... - The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular developmentOlga Kazanskaya
Division of Molecular Embryology, German Cancer Research Center, Im Neuenheimer Feld 581, D 69120 Heidelberg, Germany
Development 135:3655-64. 2008..We show that vascular endothelial growth factor is an immediate early response gene and a mediator of R-spondin signaling. The results identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis... - Targeted disruption of the Wnt regulator Kremen induces limb defects and high bone densityKristina Ellwanger
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Mol Cell Biol 28:4875-82. 2008..Our study provides the first genetic evidence for a functional interaction of Kremen proteins with Dkk1 as negative regulators of Wnt/beta-catenin signaling and reveals that Kremen proteins are not universally required for Dkk1 function... - Cell cycle control of wnt receptor activationGary Davidson
Division of Molecular Embryology, Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann von Helmholtz Platz 1, 76344 Eggenstein Leopoldshafen, Germany
Dev Cell 17:788-99. 2009..G2/M priming of LRP6 by a Cyclin/CDK complex introduces an unexpected new layer of regulation of Wnt signaling... - Global gene expression profiling and cluster analysis in Xenopus laevisDanila Baldessari
Division of Molecular Embryology, German Cancer Research Center (DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Mech Dev 122:441-75. 2005..The study provides insights into the higher order of vertebrate gene expression, identifies synexpression groups and marker genes, and makes predictions for the biological role of numerous uncharacterized genes... - Reliability of gene expression ratios for cDNA microarrays in multiconditional experiments with a reference designRainer König
Division of Theoretical Bioinformatics, German Cancer Research Center DKFZ, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Nucleic Acids Res 32:e29. 2004..Finally, we compared microarray-based gene expression profiles with the corresponding expression patterns obtained by whole-mount in situ hybridizations, resulting in a 90% correspondence... - A role for fibronectin-leucine-rich transmembrane cell-surface proteins in homotypic cell adhesionEmil E Karaulanov
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
EMBO Rep 7:283-90. 2006..Therefore, FLRT-mediated FGF signal transduction and homotypic cell sorting can be molecularly uncoupled. The results indicate that FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion... - Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1Christoph Hansis
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany
Curr Biol 14:1475-80. 2004..Dissection of the molecular machinery using a simplified assay system may aid in achieving complete nuclear reprogramming of somatic cells for regenerative medicine... - Deletion of a single allele of the Dkk1 gene leads to an increase in bone formation and bone massFrederic Morvan
Department of Cell Biology, Yale University, New Heaven, Connecticut, USA
J Bone Miner Res 21:934-45. 2006..Unexpectedly, the Wnt antagonist Dkk2 is required for terminal osteoblast differentiation and mineralized matrix formation. We show that Dkk1, unlike Dkk2, negatively regulates osteoblast differentiation and bone formation... - Developmental biology: a tale of tailsChristof Niehrs
Nature 424:375-6. 2003 - Dickkopf-1 regulates gastrulation movements by coordinated modulation of Wnt/beta catenin and Wnt/PCP activities, through interaction with the Dally-like homolog KnypekLuca Caneparo
Medical Research Council Centre for Developmental Neurobiology, King s College London, SE1 1UL London, UK
Genes Dev 21:465-80. 2007..Our data therefore indicate that Dkk1 regulates gastrulation movement through interaction with LRP5/6 and Kny and coordinated modulations of Wnt/beta catenin and Wnt/PCP pathways... - TGF-beta signaling-mediated morphogenesis: modulation of cell adhesion via cadherin endocytosisSouichi Ogata
Department of Developmental and Cell Biology, Developmental Biology Center, University of California at Irvine, Irvine, California 92697, USA
Genes Dev 21:1817-31. 2007.... - Neural crests are actively precluded from the anterior neural fold by a novel inhibitory mechanism dependent on Dickkopf1 secreted by the prechordal mesodermCarlos Carmona-Fontaine
Department of Anatomy and Developmental Biology, University College London, UK
Dev Biol 309:208-21. 2007..These results demonstrate that anterior regions of the embryo lack NC because of a mechanism, conserved from fish to mammals, that suppresses Wnt/beta-catenin signaling via Dkk1... - In situ analysis of gene expression in Xenopus embryosNicolas Pollet
Laboratoire de Transgenèse et Génétique des Amphibiens, CNRS UMR 8080, IBAIC Bat 447, Universite Paris Sud, 91405 Orsay Cedex, France
C R Biol 326:1011-7. 2003..These sets of co-regulated genes show a striking similarity with operons, and may be a key determinant facilitating evolutionary change leading to animal diversity... - Generation and characterization of dickkopf3 mutant miceIvan del Barco Barrantes
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Mol Cell Biol 26:2317-26. 2006..Altered phenotypes in dkk3 mutant mice were observed in the frequency of NK cells, immunoglobulin M, hemoglobin, and hematocrit levels, as well as lung ventilation. Furthermore, dkk3-deficient mice display hyperactivity...