F Lyko

Summary

Affiliation: German Cancer Research Center
Country: Germany

Publications

  1. ncbi request reprint Novel methods for analysis of genomic DNA methylation
    Frank Lyko
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Anal Bioanal Chem 381:67-8. 2005
  2. ncbi request reprint Epigenetics and New Therapies in Cancer--An ESO-CNIO Conference. 29-30 November 2007, Madrid, Spain
    Frank Lyko
    German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    IDrugs 11:79-81. 2008
  3. ncbi request reprint DNA methyltransferase inhibitors and the development of epigenetic cancer therapies
    Frank Lyko
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld, Heidelberg, Germany
    J Natl Cancer Inst 97:1498-506. 2005
  4. ncbi request reprint Epigenetic regulation in Drosophila
    F Lyko
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Curr Top Microbiol Immunol 310:23-44. 2006
  5. doi request reprint Modes of action of the DNA methyltransferase inhibitors azacytidine and decitabine
    Carlo Stresemann
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Int J Cancer 123:8-13. 2008
  6. ncbi request reprint Conservation of DNA methylation in dipteran insects
    J Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Insect Mol Biol 13:117-23. 2004
  7. doi request reprint Delivery of 5-azacytidine to human cancer cells by elaidic acid esterification increases therapeutic drug efficacy
    Bodo Brueckner
    Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Mol Cancer Ther 9:1256-64. 2010
  8. ncbi request reprint DNA methyltransferase inhibitors for cancer therapy
    Bodo Brueckner
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer J 13:17-22. 2007
  9. pmc Allelic silencing at the tumor-suppressor locus 13q14.3 suggests an epigenetic tumor-suppressor mechanism
    Daniel Mertens
    Department of Molecular Genetics, Deutsches Krebsforschungszentrum, INF 280, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:7741-6. 2006
  10. doi request reprint Vectorial transport of nucleoside analogs from the apical to the basolateral membrane in double-transfected cells expressing the human concentrative nucleoside transporter hCNT3 and the export pump ABCC4
    Maria Rius
    Division of Tumor Biochemistry, German Cancer Research Center, D 69120 Heidelberg, Germany
    Drug Metab Dispos 38:1054-63. 2010

Collaborators

  • Robert Brown
  • En Li
  • Matthias Schaefer
  • Stephan Stilgenbauer
  • Michael Lubbert
  • Björn Hackanson
  • Verena Beier
  • Cordula Tschuch
  • Stephan Wolf
  • Jorg D Hoheisel
  • Mark Helm
  • Helga Stopper
  • Ulrich Mahlknecht
  • C Niehrs
  • Joachim Marhold
  • S Hiendleder
  • Victor Ambros
  • E Wolf
  • A Jeltsch
  • Bodo Brueckner
  • Carlo Stresemann
  • Cora Mund
  • Tanja Musch
  • Dirk Stach
  • Maria Rius
  • Pawel Siedlecki
  • Manfred Wiessler
  • Frank Weissmann
  • Esther Schirrmacher
  • Piotr Zielenkiewicz
  • M Rius
  • Regine Garcia Boy
  • Sandor Suhai
  • B Weber
  • Daniela Keller
  • Manuela Brom
  • Dietrich Keppler
  • Sabrina Castellano
  • Dirk Kuck
  • Barbara Weber
  • Guillermo Barreto
  • Daniel Mertens
  • Mohsen Karimi
  • Markus Kuhlmann
  • Ralf Schirrmacher
  • Hartmut Dohner
  • Sylvia Erhardt
  • Renato Paro
  • Natascha Kunert
  • Maria Rivera Markelova
  • Yuva Oz
  • G Howard
  • Johanna Hummel-Eisenbeiss
  • Marit Liland Sandvold
  • S Kimhi
  • Iduna Fichtner
  • A Eden
  • Petter Arnt Hals
  • Achim Breiling
  • Imke Bokelmann
  • Gianluca Sbardella
  • Ettore Novellino
  • Marina Sala
  • Ruprecht Kuner
  • Nicole Maltry
  • Michael Meister
  • Andrea Schäfer
  • Gabi Döderlein
  • Holger Sultmann
  • Suresh K Swaminathan
  • Wei Wu
  • Vikas Handa
  • Georgy Bakalkin
  • Martin Schalling
  • Sibylle Ohl
  • Sofia Johansson
  • Frank Schmitges
  • Catharina Larsson
  • Petra Schroeter
  • Dirk Kienle
  • Carmen Beck
  • Martin Corcoran
  • Peter Bartenstein
  • Dan Grander
  • Tomas J Ekström
  • Peter Lichter
  • Pontus Larsson
  • Michael Dahms
  • Christian Hammann
  • Jianbo Na

Detail Information

Publications50

  1. ncbi request reprint Novel methods for analysis of genomic DNA methylation
    Frank Lyko
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Anal Bioanal Chem 381:67-8. 2005
  2. ncbi request reprint Epigenetics and New Therapies in Cancer--An ESO-CNIO Conference. 29-30 November 2007, Madrid, Spain
    Frank Lyko
    German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    IDrugs 11:79-81. 2008
  3. ncbi request reprint DNA methyltransferase inhibitors and the development of epigenetic cancer therapies
    Frank Lyko
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld, Heidelberg, Germany
    J Natl Cancer Inst 97:1498-506. 2005
    ....
  4. ncbi request reprint Epigenetic regulation in Drosophila
    F Lyko
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Curr Top Microbiol Immunol 310:23-44. 2006
    ....
  5. doi request reprint Modes of action of the DNA methyltransferase inhibitors azacytidine and decitabine
    Carlo Stresemann
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Int J Cancer 123:8-13. 2008
    ..We also discuss the mechanism of DNA methyltransferase inhibition and emphasize the need for the identification of predictive biomarkers for the further advancement of epigenetic therapies...
  6. ncbi request reprint Conservation of DNA methylation in dipteran insects
    J Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Insect Mol Biol 13:117-23. 2004
    ....
  7. doi request reprint Delivery of 5-azacytidine to human cancer cells by elaidic acid esterification increases therapeutic drug efficacy
    Bodo Brueckner
    Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Mol Cancer Ther 9:1256-64. 2010
    ..Together, these data represent a detailed characterization of the CP-4200 mode of action and suggest that elaidic acid modification improves the therapeutic efficacy of azacytidine...
  8. ncbi request reprint DNA methyltransferase inhibitors for cancer therapy
    Bodo Brueckner
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer J 13:17-22. 2007
    ..The use of novel DNA methyltransferase inhibitors in clinical trials that allow monitoring of drug-induced DNA methylation changes should provide the foundation for improved epigenetic cancer therapies...
  9. pmc Allelic silencing at the tumor-suppressor locus 13q14.3 suggests an epigenetic tumor-suppressor mechanism
    Daniel Mertens
    Department of Molecular Genetics, Deutsches Krebsforschungszentrum, INF 280, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:7741-6. 2006
    ..3 results in significant down-regulation of the candidate genes and loss of function, providing a model for the interaction of genetic lesions and epigenetic silencing at 13q14.3 in B cell chronic lymphocytic leukemia...
  10. doi request reprint Vectorial transport of nucleoside analogs from the apical to the basolateral membrane in double-transfected cells expressing the human concentrative nucleoside transporter hCNT3 and the export pump ABCC4
    Maria Rius
    Division of Tumor Biochemistry, German Cancer Research Center, D 69120 Heidelberg, Germany
    Drug Metab Dispos 38:1054-63. 2010
    ..The results contribute to a better understanding of the cellular transport characteristics of nucleoside drugs...
  11. doi request reprint Human concentrative nucleoside transporter 1-mediated uptake of 5-azacytidine enhances DNA demethylation
    Maria Rius
    Division of Tumor Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Mol Cancer Ther 8:225-31. 2009
    ..In conclusion, our study identifies 5-azaCyd as a novel substrate for hCNT1 and provides direct evidence that hCNT1 is involved in the DNA-demethylating effects of this drug...
  12. pmc RNA cytosine methylation analysis by bisulfite sequencing
    Matthias Schaefer
    Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Nucleic Acids Res 37:e12. 2009
    ..These results establish a versatile method for the identification and characterization of RNA methylation patterns, which will be useful for defining the biological function of RNA methylation...
  13. doi request reprint Demethylation of a LINE-1 antisense promoter in the cMet locus impairs Met signalling through induction of illegitimate transcription
    B Weber
    Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    Oncogene 29:5775-84. 2010
    ..Our results thus provide an important paradigm for demethylation-dependent modulation of gene expression, even if the promoter of the corresponding gene is unmethylated...
  14. ncbi request reprint Reactivation of epigenetically silenced genes by DNA methyltransferase inhibitors: basic concepts and clinical applications
    Cora Mund
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, Heidelberg, Germany
    Epigenetics 1:7-13. 2006
    ..Rational design of epigenetic inhibitors might provide the foundation for a broader use of these drugs in the treatment of cancer...
  15. doi request reprint Azacytidine causes complex DNA methylation responses in myeloid leukemia
    Carlo Stresemann
    Division of Epigenetics, German Cancer Research Center, Germany
    Mol Cancer Ther 7:2998-3005. 2008
    ..Our results provide important proof-of-mechanism data for the demethylating activity of azacytidine in MDS patients and provide detailed insight into drug-induced demethylation responses...
  16. pmc Array-based analysis of genomic DNA methylation patterns of the tumour suppressor gene p16INK4A promoter in colon carcinoma cell lines
    Cora Mund
    Division of Epigenetics, Deutsches Krebsforschungszentrum Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Nucleic Acids Res 33:e73. 2005
    ..At the level of individual cytosines, however, significant variations between individual cells of the same type were found, but also consistencies across the panel of cancer cell lines were observed...
  17. ncbi request reprint Non-mammalian models for epigenetic analyses in cancer
    Matthias Schaefer
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Hum Mol Genet 16:R1-6. 2007
    ..Recent findings in all three areas will improve our understanding of epigenetic misregulation in cancer and facilitate the translation of basic research concepts into clinical applications...
  18. ncbi request reprint Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation
    Guillermo Barreto
    Division of Molecular Embryology, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nature 445:671-5. 2007
    ..Active demethylation occurs by DNA repair and Gadd45a interacts with and requires the DNA repair endonuclease XPG. We conclude that Gadd45a relieves epigenetic gene silencing by promoting DNA repair, which erases methylation marks...
  19. ncbi request reprint Methylation of human microRNA genes in normal and neoplastic cells
    Barbara Weber
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cell Cycle 6:1001-5. 2007
    ..Possible explanations for this phenomenon include the specific structure of miRNA genes and/or their requirement for strict expression regulation...
  20. ncbi request reprint Functional diversity of DNA methyltransferase inhibitors in human cancer cell lines
    Carlo Stresemann
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Res 66:2794-800. 2006
    ..These results show a substantial diversity in the molecular activities of DNA methyltransferase inhibitors and provide valuable insights into the developmental potential of individual drugs...
  21. doi request reprint Azacytidine inhibits RNA methylation at DNMT2 target sites in human cancer cell lines
    Matthias Schaefer
    Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Cancer Res 69:8127-32. 2009
    ..Our results uncover a novel and quantifiable drug activity of azacytidine and raise the possibility that tRNA hypomethylation might contribute to patient responses...
  22. ncbi request reprint Characterization of DNA demethylation effects induced by 5-Aza-2'-deoxycytidine in patients with myelodysplastic syndrome
    Cora Mund
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Res 65:7086-90. 2005
    ..In addition, our data uncovers novel targets of decitabine-mediated demethylation that are important for the refinement of treatment schedules with demethylating drugs...
  23. ncbi request reprint Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases
    Bodo Brueckner
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Res 65:6305-11. 2005
    ..These results establish RG108 as a DNA methyltransferase inhibitor with fundamentally novel characteristics that will be particularly useful for the experimental modulation of epigenetic gene regulation...
  24. ncbi request reprint DNA methyltransferase inhibitors: old and new drugs for an epigenetic cancer therapy
    Bodo Brueckner
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Trends Pharmacol Sci 25:551-4. 2004
    ..This knowledge will be essential for the design of a new generation of pharmacological inhibitors that target epigenetic processes...
  25. ncbi request reprint A Dnmt2-like protein mediates DNA methylation in Drosophila
    Natascha Kunert
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Development 130:5083-90. 2003
    ..These results demonstrate that Dnmt2 is both necessary and sufficient for DNA methylation in Drosophila and suggest a novel CpT/A-specific DNA methyltransferase activity for Dnmt2 proteins...
  26. pmc Nucleoside drugs induce cellular differentiation by caspase-dependent degradation of stem cell factors
    Tanja Musch
    Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    PLoS ONE 5:e10726. 2010
    ..We therefore analysed the potential of three nucleoside analogues to induce differentiation of the embryonic cancer stem cell line NTERA 2 D1 and compared their effect to the natural ligand retinoic acid...
  27. pmc Capillary electrophoretic analysis of genomic DNA methylation levels
    Dirk Stach
    Division of Molecular Toxicology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nucleic Acids Res 31:E2. 2003
    ..Because of its high accuracy and throughput our method will be useful in determining the role of genomic DNA methylation levels in tumorigenesis...
  28. pmc RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
    Matthias Schaefer
    Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg 69120, Germany
    Genes Dev 24:1590-5. 2010
    ..These results uncover a novel biological function of Dnmt2-mediated tRNA methylation, and suggest a role for Dnmt2 enzymes during the biogenesis of tRNA-derived small RNAs...
  29. ncbi request reprint The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function
    Bodo Brueckner
    Divisions of Epigenetics and Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Cancer Res 67:1419-23. 2007
    ..Our results thus identify let-7a-3 as an epigenetically regulated miRNA gene with oncogenic function and suggest that aberrant miRNA gene methylation might contribute to the human cancer epigenome...
  30. doi request reprint Epigenetic cancer therapy: rationales, targets and drugs
    M Rius
    Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    Oncogene 31:4257-65. 2012
    ..Druggable somatic mutations in the corresponding epigenetic regulators are beginning to be identified and should facilitate the development of epigenetic therapy approaches with improved tumor specificity...
  31. ncbi request reprint Chromosomal elements conferring epigenetic inheritance
    F Lyko
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Bioessays 21:824-32. 1999
    ..Here we review recent data obtained from analyzing epigenetic gene regulation in different organisms and show that similarities in the underlying mechanisms appear to exist...
  32. ncbi request reprint DNA methylation with a sting: an active DNA methylation system in the honeybee
    Matthias Schaefer
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Bioessays 29:208-11. 2007
    ..These findings establish bees as a model to analyze the function of DNA methylation systems in invertebrate organisms and might also be important to understand evolutionary aspects of DNA methylation in higher eukaryotes...
  33. pmc DNA hypermethylation in Drosophila melanogaster causes irregular chromosome condensation and dysregulation of epigenetic histone modifications
    Frank Weissmann
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany
    Mol Cell Biol 23:2577-86. 2003
    ..Our results thus uncover the molecular consequences of genomic hypermethylation and demonstrate a mutual interaction between DNA methylation and histone methylation...
  34. ncbi request reprint Polycomb-group proteins are involved in silencing processes caused by a transgenic element from the murine imprinted H19/Igf2 region in Drosophila
    Sylvia Erhardt
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Dev Genes Evol 213:336-44. 2003
    ..Polycomb group proteins are essential for gene silencing during development. Our results indicate that Polycomb group proteins may also be involved in the regulation of mammalian imprinted genes...
  35. ncbi request reprint Quantitative analysis of DNA methylation in chronic lymphocytic leukemia patients
    Frank Lyko
    Research Group Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Electrophoresis 25:1530-5. 2004
    ..In summary, our results thus establish capillary electrophoresis as a valuable tool for analyzing the DNA methylation status of clinical samples...
  36. ncbi request reprint Cooperative interactions between epigenetic modifications and their function in the regulation of chromosome architecture
    Frank Weissmann
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Bioessays 25:792-7. 2003
    ..This view is consistent with many results from recent genomic analyses...
  37. pmc The Drosophila cytosine-5 methyltransferase Dnmt2 is associated with the nuclear matrix and can access DNA during mitosis
    Matthias Schaefer
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    PLoS ONE 3:e1414. 2008
    ..Our results represent the first comprehensive characterization of Dnmt2 proteins on the cellular level and have important implications for our understanding of the molecular activities of Dnmt2...
  38. ncbi request reprint Synthesis and in vitro evaluation of biotinylated RG108: a high affinity compound for studying binding interactions with human DNA methyltransferases
    Esther Schirrmacher
    Division of Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Bioconjug Chem 17:261-6. 2006
    ..Bio-RG108 therefore represents a suitable bioconjugate for the elucidation of inhibitory mechanisms and for the affinity purification of RG108-associated proteins...
  39. ncbi request reprint The Drosophila MBD2/3 protein mediates interactions between the MI-2 chromatin complex and CpT/A-methylated DNA
    Joachim Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Development 131:6033-9. 2004
    ..Our data provide novel insights into the function of MBD2/3 proteins and strongly suggest the existence of methylation-dependent chromatin structures in Drosophila...
  40. doi request reprint Solving the Dnmt2 enigma
    Matthias Schaefer
    Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany
    Chromosoma 119:35-40. 2010
    ..Lastly, we also discuss observations that suggest a possible role for Dnmt2 in human health and disease, which further emphasizes the importance of defining Dnmt2-modulated cellular pathways in future studies...
  41. pmc Comparative analysis of DNA methylation patterns in transgenic Drosophila overexpressing mouse DNA methyltransferases
    Cora Mund
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    Biochem J 378:763-8. 2004
    ..Our results allow a direct comparison of the activities of mammalian Dnmts and suggest a significant functional specialization of these enzymes...
  42. ncbi request reprint Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation
    Joachim Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Chromosoma 111:13-21. 2002
    ..Our results indicate that dMBD2/3 forms specialized nuclear compartments to keep certain genes epigenetically silenced during genome activation...
  43. ncbi request reprint Tissue-specific effects of in vitro fertilization procedures on genomic cytosine methylation levels in overgrown and normal sized bovine fetuses
    Stefan Hiendleder
    Institute of Molecular Animal Breeding and Biotechnology, Gene Center of the Ludwig Maximilian University, D 81377 Munich, and Pediatric Endocrinology Section, University Hospital, Tuebingen, Germany
    Biol Reprod 75:17-23. 2006
    ..Our data indicate that bovine IVF procedures can affect fetal genomic 5mC levels in a protocol- and tissue-specific manner and show that hepatic hypermethylation is associated with fetal overgrowth and its correlated endocrine changes...
  44. pmc Silencing of retrotransposons in Dictyostelium by DNA methylation and RNAi
    Markus Kuhlmann
    Abt Genetik, Universitat Kassel, Heinrich Plett Str 40, 34132 Kassel, Germany
    Nucleic Acids Res 33:6405-17. 2005
    ..The data show that DNA methylation plays a crucial role in epigenetic gene silencing in Dictyostelium but that different, partially overlapping mechanisms control transposon silencing...
  45. ncbi request reprint LUMA (LUminometric Methylation Assay)--a high throughput method to the analysis of genomic DNA methylation
    Mohsen Karimi
    Departments of Clinical Neuroscience, Karolinska Institutet, Karolinska University Hospital Solna, SE 171 76 Stockholm, Sweden
    Exp Cell Res 312:1989-95. 2006
    ..The LUMA assay may provide a useful method to analyze genome-wide DNA methylation for a variety of physiological and pathological conditions including etiologic, diagnostic and prognostic aspects of cancer...
  46. ncbi request reprint Establishment and functional validation of a structural homology model for human DNA methyltransferase 1
    Pawel Siedlecki
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Biochem Biophys Res Commun 306:558-63. 2003
    ..The resulting compound (N4-fluoroacetyl-5-azacytidine) functioned as an efficient inhibitor of DNA methylation in human tumor cell lines and also provides novel opportunities for pharmacological applications...
  47. ncbi request reprint Two substrates are better than one: dual specificities for Dnmt2 methyltransferases
    Albert Jeltsch
    School of Engineering and Science, International University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Trends Biochem Sci 31:306-8. 2006
    ..This finding has important implications for understanding the evolutionary relationships among these enzymes...
  48. ncbi request reprint Tissue-specific elevated genomic cytosine methylation levels are associated with an overgrowth phenotype of bovine fetuses derived by in vitro techniques
    Stefan Hiendleder
    Department of Molecular Animal Breeding and Biotechnology, Gene Center of the Ludwig Maximilian University, D 1377 Munich, Germany
    Biol Reprod 71:217-23. 2004
    ....
  49. ncbi request reprint Discovery of two novel, small-molecule inhibitors of DNA methylation
    Pawel Siedlecki
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    J Med Chem 49:678-83. 2006
    ..The newly discovered inhibitors validate our screening procedure and also provide a useful basis for further rational drug development...
  50. doi request reprint Constrained analogues of procaine as novel small molecule inhibitors of DNA methyltransferase-1
    Sabrina Castellano
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Salerno, Via Ponte Don Melillo, 84084 Fisciano SA, Italy
    J Med Chem 51:2321-5. 2008
    ....