Gunther Gerisch

Summary

Country: Germany

Publications

  1. doi request reprint Genetic evidence for concerted control of actin dynamics in cytokinesis, endocytic traffic, and cell motility by coronin and Aip1
    Hellen C Ishikawa-Ankerhold
    Cell Dynamics, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cytoskeleton (Hoboken) 67:442-55. 2010
  2. doi request reprint Membrane and actin reorganization in electropulse-induced cell fusion
    Gunther Gerisch
    Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Sci 126:2069-78. 2013
  3. pmc PIP3 waves and PTEN dynamics in the emergence of cell polarity
    Gunther Gerisch
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Biophys J 103:1170-8. 2012
  4. pmc Curvature recognition and force generation in phagocytosis
    Margaret Clarke
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
    BMC Biol 8:154. 2010
  5. pmc Toward the structure of dynamic membrane-anchored actin networks: an approach using cryo-electron tomography
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Adh Migr 1:145-8. 2007
  6. ncbi request reprint GFP-golvesin constructs to study Golgi tubulation and post-Golgi vesicle dynamics in phagocytosis
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Cell Biol 83:297-303. 2004
  7. ncbi request reprint Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium
    Gunther Gerisch
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Cell Biol Int 26:845-52. 2002
  8. pmc Mobile actin clusters and traveling waves in cells recovering from actin depolymerization
    Gunther Gerisch
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Biophys J 87:3493-503. 2004
  9. ncbi request reprint Cytokinesis without myosin II
    G Gerisch
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    Curr Opin Cell Biol 12:126-32. 2000
  10. ncbi request reprint Actin-binding proteins required for reliable chromosome segregation in mitosis
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Motil Cytoskeleton 57:18-25. 2004

Collaborators

Detail Information

Publications43

  1. doi request reprint Genetic evidence for concerted control of actin dynamics in cytokinesis, endocytic traffic, and cell motility by coronin and Aip1
    Hellen C Ishikawa-Ankerhold
    Cell Dynamics, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cytoskeleton (Hoboken) 67:442-55. 2010
    ..Our results prove that coronin and Aip1 are important effectors that act together in maintaining the balance of actin polymerization and depolymerization in living cells...
  2. doi request reprint Membrane and actin reorganization in electropulse-induced cell fusion
    Gunther Gerisch
    Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Sci 126:2069-78. 2013
    ..These data indicate that the membranes persist in a fusogenic state before pores of about 3 nm diameter are formed...
  3. pmc PIP3 waves and PTEN dynamics in the emergence of cell polarity
    Gunther Gerisch
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Biophys J 103:1170-8. 2012
    ..Actin clusters were remodeled into a ring along the perimeter of the expanding PIP3 wave. The reverse transition of PIP3 to PTEN was linked to the previous site of wave initiation: where PIP3 decayed first, the entry of PTEN was primed...
  4. pmc Curvature recognition and force generation in phagocytosis
    Margaret Clarke
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
    BMC Biol 8:154. 2010
    ..We survey mechanisms that enable a phagocyte to remodel actin organization in response to particles of complex shape...
  5. pmc Toward the structure of dynamic membrane-anchored actin networks: an approach using cryo-electron tomography
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Adh Migr 1:145-8. 2007
    ....
  6. ncbi request reprint GFP-golvesin constructs to study Golgi tubulation and post-Golgi vesicle dynamics in phagocytosis
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Eur J Cell Biol 83:297-303. 2004
    ..Applying this construct to a phagosome filled with multiple particles, we observed that the phagosome is segmented during exocytosis, meaning that sequential release of particles alternates with membrane fusion...
  7. ncbi request reprint Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium
    Gunther Gerisch
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Cell Biol Int 26:845-52. 2002
    ..Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man...
  8. pmc Mobile actin clusters and traveling waves in cells recovering from actin depolymerization
    Gunther Gerisch
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Biophys J 87:3493-503. 2004
    ..These data show that the initiation of actin polymerization is separated in Dictyostelium from front protrusion, and that the coupling of polymerization to protrusion is a later step in the reconstitution of a leading edge...
  9. ncbi request reprint Cytokinesis without myosin II
    G Gerisch
    Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
    Curr Opin Cell Biol 12:126-32. 2000
    ..A centripetal flow of actin filaments followed by their disassembly in the cleavage furrow is proposed to underlie the translocation...
  10. ncbi request reprint Actin-binding proteins required for reliable chromosome segregation in mitosis
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Motil Cytoskeleton 57:18-25. 2004
    ..We hypothesize that mutations in regulatory proteins of the actin network might be one cause of genetic instability of malignant tumor cells...
  11. pmc Different modes of state transitions determine pattern in the Phosphatidylinositide-Actin system
    Gunther Gerisch
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    BMC Cell Biol 12:42. 2011
    ..To study de-novo cell polarization, we first depolymerized actin and subsequently recorded the spontaneous reorganization of actin patterns in relation to PTEN...
  12. pmc Self-organizing actin waves as planar phagocytic cup structures
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Adh Migr 3:373-82. 2009
    ..We suggest that actin waves enable a phagocyte to scan a surface for particles that might be engulfed...
  13. pmc Self-organizing actin waves that simulate phagocytic cup structures
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
    PMC Biophys 3:7. 2010
    ..5) The actin wave and PIP3 patterns resemble 2-dimensional projections of phagocytic cups, suggesting that they are involved in the scanning of surfaces for particles to be taken up.PACS Codes: 87.16.Ln, 87.19.lp, 89.75.Fb...
  14. ncbi request reprint Dynamic organization of the actin system in the motile cells of Dictyostelium
    Till Bretschneider
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Muscle Res Cell Motil 23:639-49. 2002
    ..We discuss the potential of electron tomography of vitrified cells to visualize actin networks in their native association with membranes...
  15. ncbi request reprint Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cells
    Till Bretschneider
    Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
    Curr Biol 14:1-10. 2004
    ..The analysis of actin network dynamics requires the combination of high-resolution imaging with a specific fluorescent probe that highlights the filamentous actin structures in live cells...
  16. pmc Mechanically induced actin-mediated rocketing of phagosomes
    Margaret Clarke
    Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
    Mol Biol Cell 17:4866-75. 2006
    ..The forces generated by rocketing phagosomes are sufficient to push the entire microtubule apparatus forward and to dislocate the nucleus...
  17. ncbi request reprint Endosome fusion and microtubule-based dynamics in the early endocytic pathway of dictyostelium
    Margaret Clarke
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    Traffic 3:791-800. 2002
    ..Further maturation of endosomes led to loss of pleiomorphy in favor of a spherical shape, inability to fuse with new macropinosomes, and diminished motility...
  18. ncbi request reprint Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells
    Margaret Clarke
    Oklahoma Medical Research Foundation, Oklahoma City, USA
    J Cell Sci 115:2893-905. 2002
    ..VatM-GFP was retrieved from the phagosome membrane prior to exocytosis of the indigestible remnants of the yeast particle. These data suggest that vacuolar proton pumps are recycled by fusion of advanced with newly formed endosomes...
  19. ncbi request reprint Organization of actin networks in intact filopodia
    Ohad Medalia
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Curr Biol 17:79-84. 2007
    ..We hypothesize that growth of the highly dynamic filopodia of Dictyostelium is accompanied by repetitive nucleation of actin polymerization at the filopod tip, followed by the rearrangement of filaments within the shaft...
  20. pmc Retrieval of the vacuolar H-ATPase from phagosomes revealed by live cell imaging
    Margaret Clarke
    Program in Genetic Models of Disease, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America
    PLoS ONE 5:e8585. 2010
    ..Similarly, the enzyme is thought to be retrieved from phagosome membranes prior to exocytosis of indigestible material, although that process has not been directly visualized...
  21. ncbi request reprint A talin fragment as an actin trap visualizing actin flow in chemotaxis, endocytosis, and cytokinesis
    Igor Weber
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Cell Motil Cytoskeleton 53:136-49. 2002
    ..These cells are considered to mimic the phenotype of mutants deficient in the shredding of actin filaments that normally occurs in the mid-zone of a dividing cell...
  22. pmc The three-dimensional dynamics of actin waves, a model of cytoskeletal self-organization
    Till Bretschneider
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Biophys J 96:2888-900. 2009
    ..These data show that force-generating, highly organized supramolecular networks are autonomously formed in live cells from molecular motors and proteins controlling actin polymerization and depolymerization...
  23. ncbi request reprint Nuclear pore complex structure and dynamics revealed by cryoelectron tomography
    Martin Beck
    Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Science 306:1387-90. 2004
    ..Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold...
  24. ncbi request reprint Time-resolved responses to chemoattractant, characteristic of the front and tail of Dictyostelium cells
    Martin Etzrodt
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    FEBS Lett 580:6707-13. 2006
    ..The cortical accumulation of myosin-II starts when the front response has declined, supporting the concept of divergent signal transmission and adaptation pathways...
  25. doi request reprint Imaging actin cytoskeleton dynamics in Dictyostelium chemotaxis
    Gunther Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Methods Mol Biol 571:385-400. 2009
    ..Techniques of total internal reflection fluorescence (TIRF) and spinning-disc confocal microscopy provide appropriate temporal and spatial resolution for the analysis of actin dynamics...
  26. ncbi request reprint GFP-fusion proteins as fluorescent reporters to study organelle and cytoskeleton dynamics in chemotaxis and phagocytosis
    Gunther Gerisch
    Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Methods Enzymol 361:320-37. 2003
  27. ncbi request reprint Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography
    Ohad Medalia
    Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Science 298:1209-13. 2002
    ..At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment...
  28. pmc Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility
    E L de Hostos
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Cell Biol 120:163-73. 1993
    ..This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis...
  29. ncbi request reprint Golvesin-GFP fusions as distinct markers for Golgi and post-Golgi vesicles in Dictyostelium cells
    N Schneider
    Max Planck lnstitut für Biochemie, Martinsried, Germany
    Biol Cell 92:495-511. 2000
    ..The fusion protein is retained in Golgi vesicles during mitosis, visualizing Golgi disassembly and reorganization in line with cytokinesis...
  30. pmc Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis
    J Niewöhner
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Cell Biol 138:349-61. 1997
    ..With their high proportion of binucleated cells, the talin-null mutants revealed interactions of the mitotic apparatus with the cell cortex that were not obvious in mononucleated cells...
  31. ncbi request reprint Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion protein
    G Gerisch
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Curr Biol 5:1280-5. 1995
    ..It is a member of the WD-repeat family of proteins and is known to interact with actin-myosin complexes. In coronin null mutants, cell locomotion is slowed down and cytokinesis is impaired...
  32. ncbi request reprint Phosphorylation of threonine residues on cloned fragments of the Dictyostelium myosin heavy chain
    G Wagle
    Max Planck Institut fur Biochemie, Martinsried, FRG
    FEBS Lett 227:71-5. 1988
    ..Sites of phosphorylation were tentatively localized to a sequence in the C-terminal region of the heavy chain where four threonine residues are found...
  33. pmc Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis
    A Müller-Taubenberger
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    EMBO J 20:6772-82. 2001
    ....
  34. ncbi request reprint Coactosin interferes with the capping of actin filaments
    U Röhrig
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 374:284-6. 1995
    ....
  35. ncbi request reprint Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tail
    J Faix
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    Cell 86:631-42. 1996
    ..The double-mutant cells are multinucleate owing to a severe impairment of cytokinesis...
  36. pmc Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes
    J Prassler
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Mol Biol Cell 8:83-95. 1997
    ..This result shows that myosin can disperse an actin network when it acts as a motor and sheds light on the dynamics of protein-protein interactions in the cortex of a motile cell where myosin II and Dd plastin are simultaneously present...
  37. ncbi request reprint Coactosin, a 17 kDa F-actin binding protein from Dictyostelium discoideum
    E L de Hostos
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Cell Motil Cytoskeleton 26:181-91. 1993
    ..The cDNA-derived amino-acid sequence reveals similarities of coactosin to the drebrins identified in neurons and to actin-binding proteins from other organisms, including yeast ABP1p, and yeast and vertebrate cofilins...
  38. pmc Coronin, an actin binding protein of Dictyostelium discoideum localized to cell surface projections, has sequence similarities to G protein beta subunits
    E L de Hostos
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 10:4097-104. 1991
    ..It is hypothesized that coronin is implicated in the transmission of chemotactic signals from cAMP receptors in the plasma membrane through G proteins to the cortical cytoskeleton, whose structure and activity is locally modulated...
  39. pmc Drainin required for membrane fusion of the contractile vacuole in Dictyostelium is the prototype of a protein family also represented in man
    M Becker
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    EMBO J 18:3305-16. 1999
    ..We suggest that drainin acts in a signaling cascade that couples a volume-sensing device in the vacuolar membrane to the membrane fusion machinery...
  40. ncbi request reprint Proteasomes from Dictyostelium discoideum: characterization of structure and function
    T M Schauer
    Max Planck Institute for Biochemistry, Martinsried, Germany
    J Struct Biol 111:135-47. 1993
    ..The resulting model implies that the alpha- and beta-subunits have a fixed pattern of relationships. D. discoideum proteasomes are found both in the cytosol and, in higher concentrations, in the nucleus...
  41. ncbi request reprint Classification of tyrosine kinases from Dictyostelium discoideum with two distinct, complete or incomplete catalytic domains
    K Adler
    Max Planck Institut fur Biochemie, Martinsried, Germany
    FEBS Lett 395:286-92. 1996
    ..discoideum, a branch of their own, separate from the tyrosine kinase domains in sensu strictu. Domain II in DPYK4 is found on a different branch close to serine/threonine kinases...
  42. ncbi request reprint cDNA-derived sequence of UMP-CMP kinase from Dictyostelium discoideum and expression of the enzyme in Escherichia coli
    L Wiesmuller
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    J Biol Chem 265:6339-45. 1990
    ..05 and 0.1 microM) and is inactivated by modification of free thiol groups with 5,5'-dithiobis(2-nitrobenzoic acid)...
  43. pmc The hybrid histidine kinase DokA is part of the osmotic response system of Dictyostelium
    S C Schuster
    Abteilung fur Membranebiochemie, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    EMBO J 15:3880-9. 1996
    ..dokA is a member of a family of histidine kinase-like genes that play regulatory roles in eukaryotic cell function...