Wolf B Frommer

Summary

Country: Germany

Publications

  1. pmc Molecular and functional characterization of a family of amino acid transporters from Arabidopsis
    Yan Hua Su
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, 72076 Tubingen, Germany
    Plant Physiol 136:3104-13. 2004
  2. pmc Rhesus factors and ammonium: a function in efflux?
    U Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Eberhard Karls Universitat, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    Genome Biol 2:REVIEWS1010. 2001
  3. ncbi request reprint Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids
    Wolf N Fischer
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Tubingen, Germany
    Plant J 29:717-31. 2002
  4. ncbi request reprint Identification of an Arabidopsis mitochondrial succinate-fumarate translocator
    Elisabetta Catoni
    Plant Physiology, ZMBP, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    FEBS Lett 534:87-92. 2003
  5. pmc Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells
    Sylvie Lalonde
    ZMBP Tübingen, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 3:8. 2003
  6. ncbi request reprint Homo- and hetero-oligomerization of ammonium transporter-1 NH4 uniporters
    Uwe Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    J Biol Chem 278:45603-10. 2003
  7. ncbi request reprint Reduced amino acid content in transgenic potato tubers due to antisense inhibition of the leaf H+/amino acid symporter StAAP1
    Wolfgang Koch
    ZMBP, Pflanzenphysiologie, Eberhard Karls Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant J 33:211-20. 2003
  8. pmc Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll
    Axel Hirner
    Zentrum für Molekularbiologie der Pflanzen, Plant Physiology Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant Cell 18:1931-46. 2006
  9. ncbi request reprint Peptide uptake in the ectomycorrhizal fungus Hebeloma cylindrosporum: characterization of two di- and tripeptide transporters (HcPTR2A and B)
    Mariam Benjdia
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    New Phytol 170:401-10. 2006
  10. ncbi request reprint UPS1 and UPS2 from Arabidopsis mediate high affinity transport of uracil and 5-fluorouracil
    Anja Schmidt
    Plant Physiology, ZMBP, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Biol Chem 279:44817-24. 2004

Collaborators

Detail Information

Publications60

  1. pmc Molecular and functional characterization of a family of amino acid transporters from Arabidopsis
    Yan Hua Su
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, 72076 Tubingen, Germany
    Plant Physiol 136:3104-13. 2004
    ..CAT2 is localized to the tonoplast in transformed Arabidopsis protoplasts and thus may encode the long-sought vacuolar amino acid transporter...
  2. pmc Rhesus factors and ammonium: a function in efflux?
    U Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Eberhard Karls Universitat, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    Genome Biol 2:REVIEWS1010. 2001
    ..Completion of fungal, plant and human genomes paved the way to the identification of erythrocytic rhesus proteins and their kidney homologs as ammonium transporters...
  3. ncbi request reprint Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids
    Wolf N Fischer
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Tubingen, Germany
    Plant J 29:717-31. 2002
    ..Thus the actual apoplasmic concentration of amino acids and the pH will determine what is transported in vivo, i.e. major amino acids such as glutamine, asparagine, and glutamate will be mobilized preferentially...
  4. ncbi request reprint Identification of an Arabidopsis mitochondrial succinate-fumarate translocator
    Elisabetta Catoni
    Plant Physiology, ZMBP, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    FEBS Lett 534:87-92. 2003
    ..In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation...
  5. pmc Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells
    Sylvie Lalonde
    ZMBP Tübingen, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 3:8. 2003
    ..To study mechanisms for trafficking of SUT1, GFP-fusions with and without 3'-UTR were expressed in transgenic plants...
  6. ncbi request reprint Homo- and hetero-oligomerization of ammonium transporter-1 NH4 uniporters
    Uwe Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    J Biol Chem 278:45603-10. 2003
    ..Thus, in yeast, oocytes, and plants, ammonium transporters are able to oligomerize, which may be relevant for regulation of ammonium uptake...
  7. ncbi request reprint Reduced amino acid content in transgenic potato tubers due to antisense inhibition of the leaf H+/amino acid symporter StAAP1
    Wolfgang Koch
    ZMBP, Pflanzenphysiologie, Eberhard Karls Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant J 33:211-20. 2003
    ..The results demonstrate an important role for StAAP1 in long-distance transport of amino acids and highlight the importance of plasma membrane transport for nutrient distribution in plants...
  8. pmc Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll
    Axel Hirner
    Zentrum für Molekularbiologie der Pflanzen, Plant Physiology Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant Cell 18:1931-46. 2006
    ..These results suggest that LHT1 overexpression may improve the N efficiency of plant growth under limiting nitrogen, and the mutant analyses may enhance our understanding of N cycling in plants...
  9. ncbi request reprint Peptide uptake in the ectomycorrhizal fungus Hebeloma cylindrosporum: characterization of two di- and tripeptide transporters (HcPTR2A and B)
    Mariam Benjdia
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    New Phytol 170:401-10. 2006
    ..Uptake capacities and expression regulation of both genes were analysed, indicating that HcPTR2A was involved in the high-efficiency peptide uptake under conditions of limited N availability, whereas HcPTR2B was expressed constitutively...
  10. ncbi request reprint UPS1 and UPS2 from Arabidopsis mediate high affinity transport of uracil and 5-fluorouracil
    Anja Schmidt
    Plant Physiology, ZMBP, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Biol Chem 279:44817-24. 2004
    ....
  11. pmc Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system
    Waltraud X Schulze
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen ZMBP, Universitat Tubingen, Auf der Morgenstelle1, D 72076 Tubingen, Germany
    BMC Biochem 4:3. 2003
    ..The carriers differ in their kinetic properties. Many transport proteins are known to exist as oligomers. The yeast-based split ubiquitin system can be used to analyze the ability of membrane proteins to interact...
  12. ncbi request reprint Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3
    Sakiko Okumoto
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen ZMBP, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Exp Bot 55:2155-68. 2004
    ..Microarray analyses revealed that the expression profile of genes involved in amino acid metabolism did not change drastically, indicating potential compensation by other amino acid transporters...
  13. ncbi request reprint Conservation of amino acid transporters in fungi, plants and animals
    Daniel Wipf
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, Eberhard Karls Universitat Tubingen, D 72076, Tubingen, Germany
    Trends Biochem Sci 27:139-47. 2002
    ..When used in combination with phylogenetic analyses, functional comparison might aid our prediction of physiological functions for related but uncharacterized open reading frames...
  14. ncbi request reprint Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis
    Lukas Bürkle
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant J 34:13-26. 2003
    ..In summary, three closely related PUPs are differentially expressed in Arabidopsis and at least two PUPs have properties similar to the adenine and cytokinin transport system identified in Arabidopsis cell cultures...
  15. pmc K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions
    Petr Obrdlik
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 101:12242-7. 2004
    ..These results show that mbSUS is suited for systematic analysis of membrane protein interactions...
  16. pmc The role of [Delta]1-pyrroline-5-carboxylate dehydrogenase in proline degradation
    Karen Deuschle
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    Plant Cell 16:3413-25. 2004
    ..Accordingly, overexpression of AtP5CDH resulted in decreased sensitivity to externally supplied Pro. Thus, Pro and P5C/Glu semialdehyde may serve as a link between stress responses and cell death...
  17. ncbi request reprint Development of a fluorescent nanosensor for ribose
    Ida Lager
    ZMBP Tübingen, Plant Physiology, Tubingen, Germany
    FEBS Lett 553:85-9. 2003
    ..Inhibitor studies suggest that uptake is mediated by a monosaccharide transporter of the GLUT family, however, ribose taken up into the cell was not or only slowly released, indicating irreversibility of uptake...
  18. ncbi request reprint High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis
    Sakiko Okumoto
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen ZMBP, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Biol Chem 277:45338-46. 2002
    ..Overlapping substrate specificities paired with individual properties and expression patterns point to specific functions of each of the AAP genes in nitrogen distribution rather than to mere redundancy...
  19. ncbi request reprint In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors
    Marcus Fehr
    Zentrum für Molekular biologie der Pflanzen Tübingen, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Biol Chem 278:19127-33. 2003
    ..The glucose nanosensor provides a novel tool with numerous scientific, medical, and environmental applications...
  20. pmc Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis
    Elisabetta Catoni
    Plant Physiology, ZMBP, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 3:1. 2003
    ..Ort1p/Arg11p is a member of the mitochondrial carrier family (MCF) essential for ornithine export from mitochondria. The yeast arg11 mutant, which is deficient in Ort1p/Arg11p grows poorly on media lacking arginine...
  21. ncbi request reprint Plant science. Hexokinase, Jack-of-all-trades
    Wolf B Frommer
    Department of Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, D 72076 Tubingen, Germany
    Science 300:261-3. 2003
  22. ncbi request reprint Characterization of a general amino acid permease from Hebeloma cylindrosporum
    Daniel Wipf
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    FEBS Lett 528:119-24. 2002
    ..The secondary active transport mechanism together with the expression in hyphae, but not in mycorrhizas, indicate a role in uptake of organic nitrogen from the soil...
  23. ncbi request reprint Comparative studies on Ureide Permeases in Arabidopsis thaliana and analysis of two alternative splice variants of AtUPS5
    Anja Schmidt
    Center for Plant Molecular Biology, University of Tubingen, Tubingen, Germany
    Planta 224:1329-40. 2006
    ..AtUPS5s, however, could not be shown to mediate uptake of these compounds into yeast cells and might therefore be defective or have a different function...
  24. ncbi request reprint An expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum
    Daniel Wipf
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Genome 46:177-81. 2003
    ..Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning...
  25. ncbi request reprint Uniport of NH4+ by the root hair plasma membrane ammonium transporter LeAMT1;1
    Uwe Ludewig
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 1, Tubingen, Germany
    J Biol Chem 277:13548-55. 2002
    ..5 and pH 8.5. LeAMT1;1 is concluded to mediate potential-driven NH(4+) uptake and retrieval depending on root membrane potential and NH(4+) concentration gradient...
  26. pmc Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element
    Anke Reinders
    Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen, Eberhard Karls Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant Cell 14:1567-77. 2002
    ....
  27. ncbi request reprint Transport mechanisms for organic forms of carbon and nitrogen between source and sink
    Sylvie Lalonde
    ZMBP Plant Physiology, D 72076 Tubingen, Germany
    Annu Rev Plant Biol 55:341-72. 2004
    ..Novel imaging approaches may provide the means to characterize the cellular events and elucidate whole plant control of assimilate partitioning and allocation...
  28. pmc The sucrose transporter StSUT1 localizes to sieve elements in potato tuber phloem and influences tuber physiology and development
    Christina Kuhn
    Zentrum für Molekularbiologieder Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Au der Morgenstelle, Germany
    Plant Physiol 131:102-13. 2003
    ....
  29. pmc Visualization of maltose uptake in living yeast cells by fluorescent nanosensors
    Marcus Fehr
    Zentrum für Molekularbiologie der Pflanzen, Plant Physiology, Eberhard Karls Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 99:9846-51. 2002
    ..Thus the myriad of PBPs recognizing a wide spectrum of different substrates is suitable for FRET-based in vivo detection, providing numerous scientific, medical, and environmental applications...
  30. doi request reprint Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells
    Andreas Weise
    Institute of Molecular Medicine and Cell Research IMMZ, Albert Ludwigs University Freiburg, Stefan Meier Str 17, 79104 Freiburg, Germany
    Plant Mol Biol 68:251-62. 2008
    ..The combination of all introns is required for the full expression pattern in phloem, guard cells and trichomes...
  31. pmc Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
    Karen Deuschle
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Protein Sci 14:2304-14. 2005
    ..The optimization of sensors for glucose and glutamate, ligands of great clinical interest, provides a general framework for the manipulation of ligand-dependent allosteric signal transduction mechanisms...
  32. pmc GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor
    Hitomi Takanaga
    Carnegie Institution for Science, 260 Panama Street, Stanford CA 94305, USA
    Biochim Biophys Acta 1778:1091-9. 2008
    ..The improved sensitivity of the novel glucose nanosensor increases the reliability of in vivo glucose flux analyses, and provides a new means for the screening of siRNA collections as well as drugs using high-content screens...
  33. pmc Analysis of the Arabidopsis rsr4-1/pdx1-3 mutant reveals the critical function of the PDX1 protein family in metabolism, development, and vitamin B6 biosynthesis
    Susan Wagner
    Angewandte Genetik, Freie Universitat Berlin, 14195 Berlin, Germany
    Plant Cell 18:1722-35. 2006
    ..In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants...
  34. ncbi request reprint Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis
    Totte Niittyla
    Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Mol Cell Proteomics 6:1711-26. 2007
    ..Relative quantification of phosphorylation changes by phosphoproteomics provides a means for identification of fast responses to external stimuli in plants as a basis for further functional characterization...
  35. ncbi request reprint Functional expression of the green fluorescent protein in the ectomycorrhizal model fungus Hebeloma cylindrosporum
    Tobias Müller
    Transport in Ectomycorrhiza, Institute of Cellular and Molecular Botany IZMB, University Bonn, Bonn, Germany
    Mycorrhiza 16:437-42. 2006
    ..The suitability of EGFP for reporter gene studies in Hebeloma cylindrosporum was demonstrated opening up new perspectives in the Hebeloma genetics...
  36. pmc Nitrogen-dependent posttranscriptional regulation of the ammonium transporter AtAMT1;1
    Lixing Yuan
    Molecular Plant Nutrition, Institute of Plant Nutrition, University of Hohenheim, D 70593 Stuttgart, Germany
    Plant Physiol 143:732-44. 2007
    ....
  37. ncbi request reprint Heterologous expression of a plant uracil transporter in yeast: improvement of plasma membrane targeting in mutants of the Rsp5p ubiquitin protein ligase
    Marine Froissard
    Institut Jacques Monod CNRS, Université Paris VI and Paris VII, Paris, France
    Biotechnol J 1:308-20. 2006
    ..rsp5 cells are thus powerful tools to solve the many problems inherent to heterologous expression of membrane proteins in yeast, including ER retention...
  38. pmc Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants
    Karen Deuschle
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Cell 18:2314-25. 2006
    ....
  39. ncbi request reprint Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose
    Ida Lager
    Carnegie Institution, Department of Plant Biology, Stanford, California 94305, USA
    J Biol Chem 281:30875-83. 2006
    ..This novel reagent complements FLIPs for glucose, maltose, ribose, glutamate, and phosphate and will be used for analysis of sucrose-derived carbon flux in bacterial, fungal, plant, and animal cells...
  40. pmc A novel analytical method for in vivo phosphate tracking
    Hong Gu
    Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA
    FEBS Lett 580:5885-93. 2006
    ..FLIPPi sensors are suitable for real-time monitoring of P(i) metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of P(i) during cell migration...
  41. pmc Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells
    Loren L Looger
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Physiol 138:555-7. 2005
  42. pmc Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors
    Sakiko Okumoto
    Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 102:8740-5. 2005
    ..The results demonstrate that FLIPE sensors can be used for real-time monitoring of glutamate metabolism in living cells, in tissues, or in intact organisms, providing tools for studying metabolism or for drug discovery...
  43. ncbi request reprint Seed-specific overexpression of a potato sucrose transporter increases sucrose uptake and growth rates of developing pea cotyledons
    Elke Rosche
    School of Environmental and Life Sciences, The University of Newcastle, NSW 2308, Australia
    Plant J 30:165-75. 2002
    ..This indicates that the cellular location of sucrose transporter activity plays a key role in determining rates of sucrose import into cotyledons...
  44. ncbi request reprint Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays
    Anke Reinders
    Plant Biology, University of Minnesota, 220 Biological Sciences Center, 1445 Gortner Avenue, St Paul, MN 55108, USA
    Structure 10:763-72. 2002
    ..The split-ubiquitin system efficiently detects intramolecular interactions in membrane proteins, and can be used to dissect transporter structure...
  45. ncbi request reprint Plant biology: Ping-pong with boron
    Wolf B Frommer
    Nature 420:282-3. 2002
  46. ncbi request reprint Expression of the NH(+)(4)-transporter gene LEAMT1;2 is induced in tomato roots upon association with N(2)-fixing bacteria
    Dirk Becker
    Julius von Sachs Institut, Molekulare Pflanzenphysiologie and Biophysik, Julius von Sachs Platz 2, 97082 Wurzburg, Germany
    Planta 215:424-9. 2002
    ....
  47. pmc ARAMEMNON, a novel database for Arabidopsis integral membrane proteins
    Rainer Schwacke
    Universitat zu Koln, Botanisches Institut, Gyrhofstrasse 15, 50931 Koln, Germany
    Plant Physiol 131:16-26. 2003
    ..and incorporated in the ARAMEMNON DB. The ARAMEMNON DB is accessible at the URL http://aramemnon.botanik.uni-koeln.de...
  48. pmc AtDUR3 encodes a new type of high-affinity urea/H+ symporter in Arabidopsis
    Lai Hua Liu
    Institut für Pflanzenernährung, Universitat Hohenheim, D 70593 Stuttgart, Germany
    Plant Cell 15:790-800. 2003
    ..AtDUR3 was expressed in shoots and roots and upregulated during early germination and under nitrogen deficiency in roots. We propose a role of AtDUR3 in urea uptake by plant cells at low external urea concentrations...
  49. ncbi request reprint Overexpression of the sucrose transporter SoSUT1 in potato results in alterations in leaf carbon partitioning and in tuber metabolism but has little impact on tuber morphology
    Georg Leggewie
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476, Golm, Germany
    Planta 217:158-67. 2003
    ....
  50. pmc Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis
    Lai Hua Liu
    Institut für Pflanzenernährung, Universitat Hohenheim, D 70593 Stuttgart, Germany
    Plant Physiol 133:1220-8. 2003
    ..Thus, in addition to their role as water channels, TIP transporters may play a role in equilibrating urea concentrations between different cellular compartments...
  51. ncbi request reprint Expression, purification and characterization of recombinant sucrose synthase 1 from Solanum tuberosum L. for carbohydrate engineering
    Ulrike Römer
    Department of Biotechnology Biomaterial Sciences, Helmholtz Institute for Biomedical Engineering, RWTH Aachen, Germany
    J Biotechnol 107:135-49. 2004
    ..3mM), an analogue of the natural substrate D-fructopyranoside. The broad substrate spectrum renders SuSy1 from potato a versatile biocatalyst for carbohydrate engineering...
  52. ncbi request reprint Live imaging of glucose homeostasis in nuclei of COS-7 cells
    Marcus Fehr
    Carnegie Institution of Washington, Plant Biology, 260 Panama Street, Stanford, California 94305 4101, USA
    J Fluoresc 14:603-9. 2004
    ....
  53. ncbi request reprint Metabolic engineering of plants: the role of membrane transport
    Reinhard Kunze
    Botanical Institute, University of Cologne, Gyrhofstrasse 15, 50931 Cologne, Germany
    Metab Eng 4:57-66. 2002
    ....
  54. pmc Overexpression of GLUTAMINE DUMPER1 leads to hypersecretion of glutamine from Hydathodes of Arabidopsis leaves
    Guillaume Pilot
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, D 72076 Germany
    Plant Cell 16:1827-40. 2004
    ..Selective secretion of Gln by the leaves may be explained by an enhanced release of this amino acid from cells. GDU1 study may help to shed light on the secretory mechanisms for amino acids in plants...
  55. ncbi request reprint Plant biology. A plant ABC transporter takes the lotus seat
    Burkhard Schulz
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    Science 306:622-5. 2004
  56. ncbi request reprint Minimally invasive dynamic imaging of ions and metabolites in living cells
    Marcus Fehr
    Carnegie Institution of Washington, Plant Biology, 260 Panama Street, Stanford, California 94305, USA
    Curr Opin Plant Biol 7:345-51. 2004
    ..A major challenge for the future is the application of these nanosensors to quantify metabolite levels in plant cells and tissues...
  57. pmc Evidence for high-capacity bidirectional glucose transport across the endoplasmic reticulum membrane by genetically encoded fluorescence resonance energy transfer nanosensors
    Marcus Fehr
    Carnegie Institution, Stanford, CA 94305, USA
    Mol Cell Biol 25:11102-12. 2005
    ..The presence of a high-capacity glucose transport system on the ER membrane is consistent with the hypothesis that glucose export from hepatocytes occurs via the cytosol by a yet-to-be-identified set of proteins...
  58. pmc Shining light on signaling and metabolic networks by genetically encoded biosensors
    Sylvie Lalonde
    Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, California 94305, USA
    Curr Opin Plant Biol 8:574-81. 2005
    ..They represent the basis for cell-based high-throughput assays that are necessary for a systems perspective on plant cell function...
  59. doi request reprint Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations
    Sylvie Lalonde
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Plant J 53:610-35. 2008
    ..597-609). In addition, we discuss the use of interaction data to construct interaction networks and as the basis for the exciting possibility of using to predict interaction surfaces...
  60. pmc Fluxomics: mass spectrometry versus quantitative imaging
    Wolfgang Wiechert
    University of Siegen, Paul Bonatz Strasse 9 11, 57068 Siegen, Germany
    Curr Opin Plant Biol 10:323-30. 2007
    ..Both methods provide high time resolution. The major advantages of FRET nanosensors are that they yield data with cellular and subcellular resolution and the method is minimally invasive...