Jurgen Popp

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..This capability with the possibility of accurate and rapid blood cell identification in cerebrospinal fluid is extremely promising for implementation in clinical diagnostics...

..Independent cultural replicates of either ampicillin-challenged or non-challenged cultures are successfully identified with identification rates of over 90%...

..The classification utilizing a tree-like classifier is also applied to the Streptomyces single-cell spectra, resulting in classification accuracies between 80 and 93% for the investigated Streptomyces species...

..In this contribution, we present a new approach to achieve reliable and robust measurements based on segmented flow LOC-SERS via online-wavenumber calibration...

..Implementation into microfluidic environments enables a high degree of automation that is required to improve the throughput of the approach for Raman activated cell sorting...

..Furthermore, the ellipticity in the BCP of the py-ph bond is related to the corresponding geometrical properties (e.g., bond length and dihedral angle) and to the DFT-calculated HOMO and LUMO energies...

..To benefit from synergies, SERS is combined with other methods, such as scanning probe microscopy and microfluidics, which illustrates the broad applications of this powerful technique...

..The suggested GA procedure results in models, which have a higher precision and are more stable against corrupting effects...

..Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented...

..Within this contribution the potential of our approach of bacterial identification for future application is discussed, focusing on the time-benefit and the combination with other microfluidic applications...

..The measurements we performed at the single-cell level thus allow fast identification within a few hours without a demanding process for sample preparation...

..Protein structures (cytochrome c) were visualized by TERS measurements on whole mitochondria referring to specific spectral features describing the electronic state of the heme moiety...

..However, higher binding rates can be achieved when using amino-modified oligonucleotides on an epoxy surface. The limit of detection for the used settings was 5 pM...

..Within this contribution, previous reported applications on droplet-based flow-through Raman and SERS approaches and the additional benefit with regard to the importance in the field of analytics are considered...

..Thus, by structurally tuning the electronic coupling of the ππ* and the MLCT states a new synthetic route toward white emitters, which can subsequently be incorporated into polymers, is opened...

..Complementary IR spectroscopy is performed for an investigation of vibrations of polar functional groups of the halofantrine molecule...

..The results shown open the way to more flexible use of plasmonic arrays in bioanalytics...

..A detection limit of 250 ng g⁻¹ for norbixin and 989 ng g⁻¹ for tartrazine in solid sugar samples could be reached by logistic regression...

..commune or other basidiomycetous fungi, by means of resonance Raman microspectroscopy and CARS microscopy. These Raman methods bear the potential of label-free in vivo mitochondria localization and investigation...

..Although the presence of PHB induced severe changes in the Raman spectra, our results suggest that Raman spectroscopy can be successfully used for identification as long as the bacteria are not in the stationary phase...

..Thereby we were able to differentiate treated from untreated MCF-7 cells and to quantify the cell-drug response by utilizing linear discriminant analysis models...

..The possible relevance of this result for the catalytic reaction in the enzyme POR is discussed...

..The model was successfully applied to identify single cells from an independent mixture of cells based on their vibrational spectra. The classification was confirmed by fluorescence staining of the cells after the Raman measurement...

..With the new concept of binding the label molecule directly to the analyte molecule, the application of linkage compounds like antibodies or any other recognition molecules becomes dispensable...

..This marks a promising step toward automated Raman spectroscopic blood analysis which holds the potential not only to assign the numbers of the cells but also to yield important biochemical information...

..aff. deliciosa). We anticipate that the work presented will open a doorway for online monitoring of formation and alterations of epicuticular wax layers...

..The living cells were identified by means of Raman spectroscopy in combination with a support vector machine. Furthermore, the localization of bacterial cells in a mix of abiotic particles is demonstrated...

..Thus, the complexes reveal great potential for dye-sensitized solar-cell applications...

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..The results are compared for both unmixing algorithms and with previously reported supervised spectral decomposition techniques...

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..Since a spatial differentiation between different compositional domains is difficult to achieve by topographic features only, the combination of morphology and spectral data enables a much more detailed characterization of biomembranes...

..Finally, the examination of the molecular orbitals and of the RR spectra for the MLCT state shows that protonation favors a charge transfer excitation to the 4H-imidazole ligand...

..The observed biological variability of the amygdalin distribution cannot yet be explained satisfactorily and requires further investigation...

..The observation of pump-intensity dependent triplet-triplet annihilation highlights the importance of considering various loss mechanisms in the design of artificial photosynthetic assemblies...

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..With this setup, we are able to distinguish between different complementary and non-complementary target sequences in one sample solution...

..meningitidis cells from patients with bacterial meningitis could be achieved...