Udo R Markert
Affiliation: Friedrich Schiller University
Johnson S, Groten T, Pastuschek J, Rödel J, Sammer U, Markert U. Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue. Placenta. 2018;67:8-14 pubmed publisher
..The commonly used protocols for preparation of placenta for its individual oral ingestion reduce hormone concentrations and bacterial contamination. ..
Morales Prieto D, Herrmann J, Osterwald H, Kochhar P, Schleussner E, Markert U, et al
. Comparison of dienogest effects upon 3,3'-diindolylmethane supplementation in models of endometriosis and clinical cases. Reprod Biol. 2018;18:252-258 pubmed publisher
..In conclusion, addition of DIM enhances effects of DNG ex vivo and may ameliorate bleeding patterns in endometriosis patients. ..
Ospina Prieto S, Chaiwangyen W, Pastuschek J, Schleussner E, Markert U, Morales Prieto D. STAT5 is Activated by Epidermal Growth Factor and Induces Proliferation and Invasion in Trophoblastic Cells. Reprod Sci. 2015;22:1358-66 pubmed publisher
..These results demonstrate that STAT5 activated by EGF constitutes an important cascade for the regulation of cell proliferation and invasion in trophoblast cells. ..
Chaiwangyen W, Ospina Prieto S, Photini S, Schleussner E, Markert U, Morales Prieto D. Dissimilar microRNA-21 functions and targets in trophoblastic cell lines of different origin. Int J Biochem Cell Biol. 2015;68:187-96 pubmed publisher
..Furthermore, miR-21 regulates major cellular processes including cell growth, migration, invasion and apoptosis suggesting that its impairment may lead to placental disorders. ..
Pastuschek J, Poetzsch J, Morales Prieto D, SchleuÃŸner E, Markert U, Georgiev G. Stimulation of the JAK/STAT pathway by LIF and OSM in the human granulosa cell line COV434. J Reprod Immunol. 2015;108:48-55 pubmed publisher
..We found constitutive protein expression for GP130, STAT3, pSTAT3(ser727) and upregulation of pSTAT3(tyr705) by LIF and OSM. Our results demonstrate the activation of the JAK/STAT pathway by LIF and OSM in human granulosa cells. ..
Froehlich K, Haeger J, Héger J, Pastuschek J, Photini S, Yan Y, et al
. Generation of Multicellular Breast Cancer Tumor Spheroids: Comparison of Different Protocols. J Mammary Gland Biol Neoplasia. 2016;21:89-98 pubmed
..5 % Matrigel), while SK-BR-3 did not under neither condition. Therefore, we outline specific methods and recommend the use of adapted and standardized spheroid generation protocols for each cell line. ..
Ospina Prieto S, Chaiwangyen W, Herrmann J, Groten T, Schleussner E, Markert U, et al
. MicroRNA-141 is upregulated in preeclamptic placentae and regulates trophoblast invasion and intercellular communication. Transl Res. 2016;172:61-72 pubmed publisher
..Furthermore, elevated levels of miR-141 can be transferred from trophoblast to immune cells by release and internalization of EVs suggesting their role in the immune regulation of normal and pathologic pregnancies. ..
Heger J, Froehlich K, Pastuschek J, Schmidt A, Baer C, Mrowka R, et al
. Human serum alters cell culture behavior and improves spheroid formation in comparison to fetal bovine serum. Exp Cell Res. 2018;365:57-65 pubmed publisher
..We were able to demonstrate that HS and FBS differently influence behavior of cells in culture which may have an impact on experimental results, especially in 3D cultures. ..
Mary Photini S, Chaiwangyen W, Weber M, Al Kawlani B, Favaro R, Jeschke U, et al
. PIM kinases 1, 2 and 3 in intracellular LIF signaling, proliferation and apoptosis in trophoblastic cells. Exp Cell Res. 2017;359:275-283 pubmed publisher
..Simultaneously, phosphorylation of c-MYC was reduced. These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells. ..