Michael Kiehntopf

Summary

Affiliation: Friedrich Schiller University
Country: Germany

Publications

  1. ncbi request reprint Application of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 high volume clinical chemistry analyzer
    Klas Boer
    Institut fur Klinische Chemie und Labordiagnostik FSU Jena, Germany
    Clin Biochem 39:1041-3. 2006
  2. ncbi request reprint Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations
    Michael Kiehntopf
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Jena, Germany
    Clin Chem Lab Med 45:1435-49. 2007
  3. pmc Postprandial effects of calcium phosphate supplementation on plasma concentration-double-blind, placebo-controlled cross-over human study
    Ulrike Trautvetter
    Department of Nutritional Physiology, Institute of Nutrition, Friedrich Schiller University of Jena, Dornburger Strasse 24, Jena D 07743, Germany
    Nutr J 12:30. 2013
  4. doi request reprint Mass spectometry-based protein patterns in the diagnosis of sepsis/systemic inflammatory response syndrome
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Friedrich Schiller University Jena, Germany
    Shock 36:560-9. 2011
  5. ncbi request reprint Analysis of the CTNS gene in patients of German and Swiss origin with nephropathic cystinosis
    Michael Kiehntopf
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Germany
    Hum Mutat 20:237. 2002
  6. doi request reprint Differentiation of Campylobacter species by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany
    Foodborne Pathog Dis 8:875-85. 2011
  7. doi request reprint Biobanking and international interoperability: samples
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, 07740 Jena, Germany
    Hum Genet 130:369-76. 2011
  8. doi request reprint Comparison of the ecarin chromogenic assay and different aPTT assays for the measurement of argatroban concentrations in plasma from healthy individuals and from coagulation factor deficient patients
    Robert Siegmund
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Jena, Germany
    Thromb Res 123:159-65. 2008
  9. doi request reprint Gene polymorphisms in the heme degradation pathway and outcome of severe human sepsis
    Christoph Sponholz
    Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany
    Shock 38:459-65. 2012
  10. pmc Targeted metabolomics for discrimination of systemic inflammatory disorders in critically ill patients
    Diana Schmerler
    Department of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Jena, Germany
    J Lipid Res 53:1369-75. 2012

Detail Information

Publications42

  1. ncbi request reprint Application of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 high volume clinical chemistry analyzer
    Klas Boer
    Institut fur Klinische Chemie und Labordiagnostik FSU Jena, Germany
    Clin Biochem 39:1041-3. 2006
    ..Evaluation of the performance of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 analyzer...
  2. ncbi request reprint Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations
    Michael Kiehntopf
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Jena, Germany
    Clin Chem Lab Med 45:1435-49. 2007
    ..In the meantime, further evaluation and optimization of both technique and marker validation strategies are called for before MS-based proteomic algorithms can be translated into routine laboratory testing...
  3. pmc Postprandial effects of calcium phosphate supplementation on plasma concentration-double-blind, placebo-controlled cross-over human study
    Ulrike Trautvetter
    Department of Nutritional Physiology, Institute of Nutrition, Friedrich Schiller University of Jena, Dornburger Strasse 24, Jena D 07743, Germany
    Nutr J 12:30. 2013
    ..The aim of the present study was to examine the postprandial calcium and phosphate concentrations after supplementation with pentacalcium hydroxy-triphosphate (CaP)...
  4. doi request reprint Mass spectometry-based protein patterns in the diagnosis of sepsis/systemic inflammatory response syndrome
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Friedrich Schiller University Jena, Germany
    Shock 36:560-9. 2011
    ..Identification of discriminatory proteins suggests a direct link between infectious-related protease activity and a sepsis-specific diagnostic pattern for discrimination of patients with SIRS...
  5. ncbi request reprint Analysis of the CTNS gene in patients of German and Swiss origin with nephropathic cystinosis
    Michael Kiehntopf
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Germany
    Hum Mutat 20:237. 2002
    ..These data provide a basis for routine molecular diagnosis of cystinosis in the central European population, especially in cystinosis patients of German and Swiss origin...
  6. doi request reprint Differentiation of Campylobacter species by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany
    Foodborne Pathog Dis 8:875-85. 2011
    ....
  7. doi request reprint Biobanking and international interoperability: samples
    Michael Kiehntopf
    Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, 07740 Jena, Germany
    Hum Genet 130:369-76. 2011
    ..Again, it may be advisable for biobank partners to claim contractual warranties for the type and quality of the biomaterial they wish to acquire...
  8. doi request reprint Comparison of the ecarin chromogenic assay and different aPTT assays for the measurement of argatroban concentrations in plasma from healthy individuals and from coagulation factor deficient patients
    Robert Siegmund
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Jena, Germany
    Thromb Res 123:159-65. 2008
    ....
  9. doi request reprint Gene polymorphisms in the heme degradation pathway and outcome of severe human sepsis
    Christoph Sponholz
    Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany
    Shock 38:459-65. 2012
    ....
  10. pmc Targeted metabolomics for discrimination of systemic inflammatory disorders in critically ill patients
    Diana Schmerler
    Department of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Jena, Germany
    J Lipid Res 53:1369-75. 2012
    ..Considering the well known pathophysiological relevance of lipid induction by bacterial components, metabolites as identified in this study are promising biomarker candidates in the differential diagnosis of SIRS and sepsis...
  11. doi request reprint Effect of empirical treatment with moxifloxacin and meropenem vs meropenem on sepsis-related organ dysfunction in patients with severe sepsis: a randomized trial
    Frank M Brunkhorst
    Department of Anesthesiology and Intensive Care Medicine, Friedrich Schiller University, Jena, Germany
    JAMA 307:2390-9. 2012
    ..Early appropriate antimicrobial therapy leads to lower mortality rates associated with severe sepsis. The role of empirical combination therapy comprising at least 2 antibiotics of different mechanisms remains controversial...
  12. doi request reprint A homemade MLPA assay detects known CTNS mutations and identifies a novel deletion in a previously unresolved cystinosis family
    Michael Kiehntopf
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Uniklinikum Jena, Jena, Germany
    Gene 495:89-92. 2012
    ..More generally, our study exemplifies the use of synthetic, homemade MLPA probesets as cheap, efficient, and rapidly available screening tools for small genes and/or very rare diseases...
  13. doi request reprint CD62L on neutrophil granulocytes, a useful, complementary marker for the prediction of ventriculitis in blood-containing CSF
    Klas Boer
    Institut für Klinische Chemie und Labordiagnostik, Friedrich Schiller University Jena, Jena, Germany
    Clin Biochem 43:1351-5. 2010
    ..Therefore CD62L expression on neutrophils may present a complementary marker to distinguish between patients with residual blood and infection...
  14. doi request reprint Searching biomarker candidates in serum using multidimensional native chromatography. II Method evaluation with Alport syndrome and severe inflammation
    Adrienne Baum
    Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, Jena, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 876:31-40. 2008
    ....
  15. doi request reprint Comparison of six D-dimer assays for the detection of clinically suspected deep venous thrombosis of the lower extremities
    Klas Boeer
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Erlanger Allee 101, Jena, Germany
    Blood Coagul Fibrinolysis 20:141-5. 2009
    ..This points to the fact that the manufacturers should more regularly review studies on the performance of their respective assays to widen the data basis for their recommended cut-offs and increase assay performance...
  16. doi request reprint In patients with aortic stenosis increased flow-mediated dilation is independently associated with higher peak jet velocity and lower asymmetric dimethylarginine levels
    Julia Schumm
    Department of Internal Medicine I, Friedrich Schiller University, Jena, Germany
    Am Heart J 161:893-9. 2011
    ..Endothelial function represents a crucial factor for the regulation of vascular tonus and its malfunction influences the formation of thrombosis and inflammation. However, the role of endothelial dysfunction in AS remains unclear...
  17. doi request reprint Microvascular tissue perfusion is impaired in acutely decompensated heart failure and improves following standard treatment
    Alexander Lauten
    Department of Internal Medicine I Cardiology, Angiology, Pneumology, Friedrich Schiller University, Erlanger Allee 101, Jena, Germany
    Eur J Heart Fail 13:711-7. 2011
    ..Therefore, the purpose of this study was to evaluate microvascular flow index (MFI) in patients with ADHF and to assess the effect of standard pharmacological therapy using Sidestream Dark Field (SDF) imaging...
  18. doi request reprint Immediate diagnosis of ventriculits: evaluation of parameters independent of microbiological culture
    Klas Boeer
    Institut fuer Klinische Chemie und Laboratoriumsdiagnostik, FSU Jena, Germany
    Acta Neurochir (Wien) 153:1797-805. 2011
    ..We sought to improve the diagnostics by evaluating a cytokine panel and soluble CD62L as markers of ventriculitis. In addition, we tested an algorithm of established parameters to predict ventriculitis in a specific patient collective...
  19. doi request reprint Results of comprehensive geriatric assessment effect survival in patients with malignant lymphoma
    Nils Winkelmann
    Klinik fur Innere Medizin II, Abteilung Hämatologie und internistische Onkologie, Universitatsklinikum Jena, Erlanger Allee 101, 07747 Jena, Germany
    J Cancer Res Clin Oncol 137:733-8. 2011
    ..However, the prognostic value in patients with ML is unclear. We sought to establish a relationship between results of CGA and survival time in patients with ML...
  20. doi request reprint Is successful electroconvulsive therapy related to stimulation of the vagal system?
    Karl Jürgen Bär
    Department of Psychiatry and Psychotherapy, University Hospital, Jena, Germany
    J Affect Disord 125:323-9. 2010
    ..While the exact underlying mechanisms are under debate, a role for the sympathetic response upon ECT has been suggested. When assessing patients with MDD for autonomic function, however, a loss of vagal function is prominent...
  21. ncbi request reprint Putting science to work: novel diagnostic strategies in laboratory medicine
    Thomas Deufel
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, D 07740 Jena, Germany
    Expert Rev Mol Diagn 6:9-14. 2006
  22. doi request reprint Toward a spectroscopic hemogram: Raman spectroscopic differentiation of the two most abundant leukocytes from peripheral blood
    Anuradha Ramoji
    Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany
    Anal Chem 84:5335-42. 2012
    ..This marks a promising step toward automated Raman spectroscopic blood analysis which holds the potential not only to assign the numbers of the cells but also to yield important biochemical information...
  23. ncbi request reprint Identification of nuclear localisation sequences in spastin (SPG4) using a novel Tetra-GFP reporter system
    Christian Beetz
    Institut fur Pathologie, Klinikum der Friedrich Schiller Universitat Jena, Ziegelmühlenweg 1, 07740 Jena, Germany
    Biochem Biophys Res Commun 318:1079-84. 2004
    ..Using this novel tool we demonstrate that spastin carries two NLSs located in exons 1 and 6. Both are independently functional in mediating nuclear entry...
  24. doi request reprint Lactic acid is of low predictive value for the diagnosis of bacterial infection in ventricular cerebrospinal fluid samples containing residual blood
    Klas Boer
    Institut fuer Klinische Chemie und Laboratoriumsdiagnostik, FSU Jena, Jena, Germany
    Clin Chem Lab Med 48:1777-80. 2010
    ..Lactic acid concentrations (LA) are an established marker of bacterial infection in cerebrospinal fluid (CSF). However, use of LA for the detection of infection in CSF with residual blood has not been fully evaluated...
  25. doi request reprint Influence of direct thrombin inhibitor argatroban on coagulation assays in healthy individuals, patients under oral anticoagulation therapy and patients with liver dysfunction
    Robert Siegmund
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Jena, Germany
    Blood Coagul Fibrinolysis 19:288-93. 2008
    ..Argatroban had no influence on chromogenic protein C measurement, batroxobin time and induced platelet aggregation. Knowledge of argatroban interference is a prerequisite for the reliable interpretation of coagulation assays...
  26. doi request reprint Prognostic impact of pretherapeutic laboratory values in head and neck cancer patients
    Franziska Peter
    Department of Otorhinolaryngology, Jena University Hospital, Lessingstrasse 2, 07740 Jena, Germany
    J Cancer Res Clin Oncol 139:171-8. 2013
    ..We investigated whether HNC and its comorbidity also influence routine laboratory values and whether these values have influence on overall survival...
  27. doi request reprint Hepatic induction of cholesterol biosynthesis reflects a remote adaptive response to pneumococcal pneumonia
    Martina Weber
    Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Jena, Germany
    FASEB J 26:2424-36. 2012
    ..Cholesterol may attenuate disease severity by neutralizing pneumolysin in the alveolar compartment and thus prevent septic disease progression...
  28. doi request reprint Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)
    Anne März
    Institute of Physical Chemisty, Friedrich Schiller University, Jena, Germany
    Anal Bioanal Chem 400:2755-61. 2011
    ..Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented...
  29. doi request reprint [Prevalence of polyglandular autoimmune syndrome in patients with diabetes mellitus type 1]
    Wilgard Hunger-Battefeld
    Klinik fur Innere Medizin III, Universitatsklinikum Jena, Jena
    Med Klin (Munich) 104:183-91. 2009
    ....
  30. doi request reprint Evaluation of two different albumin depletion strategies for improved analysis of human CSF by SELDI-TOF-MS
    Robert Siegmund
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, Erlanger Allee 101, D 07740 Jena, Jena, Germany
    Clin Biochem 42:1136-43. 2009
    ..Montage Albumin Deplete kit, were evaluated for improving protein profiling pattern and reproducibility in SELDI analysis...
  31. ncbi request reprint Rapid generation of detailed loss of heterozygosity profiles for routine diagnosis of gliomas
    Christian Beetz
    Institut fur Pathologie, Klinikum der Friedrich Schiller Universitat Jena, Jena, Germany
    Clin Chem Lab Med 42:595-601. 2004
    ..Our approach should prove useful in the routine diagnosis of gliomas. Simultaneously, research data for many highly relevant regions are generated in a comparatively simple and inexpensive way...
  32. ncbi request reprint Variables of host response in saliva of patients with periodontitis: a pilot study
    Arndt Guentsch
    Department of Conservative Dentistry, Jena University Hospital, Jena, Germany
    Quintessence Int 43:e104-14. 2012
    ..This study analyzed saliva samples of patients with periodontitis for several biomarkers of host response...
  33. doi request reprint Immature platelet fraction as marker for platelet recovery after stem cell transplantation in children
    Eva Hennel
    Institut fur Klinische Chemie und Laboratoriumsmedizin, Jena University Hospital, Friedrich Schiller University, Jena, Germany
    Clin Biochem 45:749-52. 2012
    ..Monitoring immature platelet (IPF) fraction has been proposed to predict platelet recovery thereby reducing the need for transfusions...
  34. doi request reprint Intensive insulin therapy and pentastarch resuscitation in severe sepsis
    Frank M Brunkhorst
    Department of Anesthesiology and Intensive Care Medicine, Friedrich Schiller University, Jena, Germany
    N Engl J Med 358:125-39. 2008
    ..The role of intensive insulin therapy in patients with severe sepsis is uncertain. Fluid resuscitation improves survival among patients with septic shock, but evidence is lacking to support the choice of either crystalloids or colloids...
  35. pmc Reduced cortical thickness is associated with the glutamatergic regulatory gene risk variant DAOA Arg30Lys in schizophrenia
    C Christoph Schultz
    Department of Psychiatry and Psychotherapy, Friedrich Schiller University Jena, Jena, Germany
    Neuropsychopharmacology 36:1747-53. 2011
    ..Our finding thus underlines the importance of DAOA and related glutamatergic processes as a putative target for therapeutic interventions in schizophrenia...
  36. ncbi request reprint Gene for integrin-associated protein (IAP, CD47): physical mapping, genomic structure, and expression studies in skeletal muscle
    Jörg Schickel
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Klinikum der Friedrich Schiller Universitat Jena, Germany
    Biochem Cell Biol 80:169-76. 2002
    ..Our expression studies demonstrate expression of integrin-associated protein in the t-tubular system and the euchromatin of skeletal muscle cells where its function thus far is not known...
  37. doi request reprint Direct analysis of clinical relevant single bacterial cells from cerebrospinal fluid during bacterial meningitis by means of micro-Raman spectroscopy
    Michaela Harz
    Institute of Physical Chemistry, Friedrich Schiller Universitat Jena, Helmholtzweg 4, Jena, Germany
    J Biophotonics 2:70-80. 2009
    ..meningitidis cells from patients with bacterial meningitis could be achieved...
  38. ncbi request reprint Automated monitoring of C2 and C0 blood levels of mycophenolic acid and cyclosporine on the Abbott Architect c8000
    Klas Boer
    Institut fuer Klinische Chemie und Labordiagnostik FSU, Jena, Germany
    Clin Biochem 40:1163-7. 2007
    ..Evaluation of the performance of the EMIT 2000 Cyclosporin assay using 2 sets of assay calibrators and the EMIT 2000 mycophenolic acid assay to measure C0 and C2 concentrations on the Abbott Architect c8000 analyzer...
  39. doi request reprint [Uncontrolled clinical study of the efficacy of ambulant fasting in patients with osteoarthritis]
    Sebastian Schmidt
    Kompetenzzentrum Naturheilverfahren, Klinik fur Innere Medizin II, Universitatsklinikum Jena, Jena, Deutschland
    Forsch Komplementmed 17:87-94. 2010
    ..To study the efficacy of fasting therapy according to Buchinger on pain, state of health, and articular function in patients with osteoarthritis...
  40. ncbi request reprint Outcome-based profiling of astrocytic tumours identifies prognostic gene expression signatures which link molecular and morphology-based pathology
    Christian Beetz
    Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universitatsklinikum Jena, D 07740 Jena, Germany
    Int J Oncol 29:1183-91. 2006
    ....
  41. ncbi request reprint Integrated analysis of the cerebrospinal fluid peptidome and proteome
    Alexandre Zougman
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Proteome Res 7:386-99. 2008
    ..The CSF proteome has only partial overlap with the plasma proteome, thus it is produced locally rather than deriving from plasma. Our work offers insights into CSF composition and origin...
  42. ncbi request reprint End-stage renal failure as manifestation of adolescent cystinosis
    Michaela Marx
    Department of Paediatrics and Adolescent Medicine, Friedrich Alexander University, Loschgestrasse 25, 91054 Erlangen, Germany
    Eur J Pediatr 163:260-1. 2004