Research Topics
| Jörg SoppaSummaryAffiliation: Frankfurt am Main Country: Germany Publications
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Detail Information
Publications
The TATA-box-binding protein (TBP) of Halobacterium salinarum. Cloning of the tbp gene, heterologous production of TBP and folding of TBP into a native conformationJ Soppa
Max Planck Institut fur Biochemie, Martinsried, Germany
Eur J Biochem 249:318-24. 1997..Properly folded TBP was shown to bind to a halobacterial TATA-box-containing DNA fragment, indicating that the fusion protein can be used to characterize DNA recognition by the halobacterial TBP...- Transcription initiation in Archaea: facts, factors and future aspectsJ Soppa
Universitat Frankfurt, Biozentrum Niederursel, Institut fur Mikrobiologie, Germany
Mol Microbiol 31:1295-305. 1999..Furthermore, additional basal transcription factors and promoter elements seem to be crucial in subgroups of Archaea. Finally, some aspects of global as well as gene-specific transcriptional regulation are discussed... - Prokaryotic structural maintenance of chromosomes (SMC) proteins: distribution, phylogeny, and comparison with MukBs and additional prokaryotic and eukaryotic coiled-coil proteinsJ Soppa
Institut fur Mikrobiologie, Biozentrum Niederursel, Marie Curie Strasse 9, D 60439 Frankfurt, Germany
Gene 278:253-64. 2001..coli. A phylogenetic analysis was performed including the prokaryotic coiled-coil proteins as well as SMCs and Rad18 proteins from selected eukaryotes... - Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpBJörg Soppa
J W Goethe Universitat, Biozentrum, Institut fur Mikrobiologie, Franfurt, Germany
Mol Microbiol 45:59-71. 2002.... - From replication to cultivation: hot news from HaloarchaeaJörg Soppa
Goethe University, Biocentre, Institute for Microbiology, Marie Curie Str 9, D 60439, Germany
Curr Opin Microbiol 8:737-44. 2005..Halophilic Archaea were found in low-salt environments and are thus more widespread than previously thought... - From genomes to function: haloarchaea as model organismsJörg Soppa
Goethe University, Biocentre, Institute for Microbiology, D 60439 Frankfurt, Germany
Microbiology 152:585-90. 2006..Taken together, the wealth of techniques available make haloarchaea excellent archaeal model species... 
Small RNAs of the halophilic archaeon Haloferax volcaniiJörg Soppa
Goethe University, Biocentre, Institute of Molecular Biosciences, Max von Laue Strasse 9, D 60438 Frankfurt, Germany
Biochem Soc Trans 37:133-6. 2009..Taken together, it could be shown that sRNAs are as abundant in H. volcanii as they are in well-studied bacterial species and that they fulfil important biological roles under specific conditions...- Bioinformatic prediction and experimental verification of sRNAs in the haloarchaeon Haloferax volcaniiJulia Babski
Institute for Molecular Biosciences, Biocentre, Goethe University, Frankfurt, Germany
RNA Biol 8:806-16. 2011..Further analyses of the biological functions of selected sRNAs, including the construction of deletion mutants, are currently under way... - A 2-oxoacid dehydrogenase complex of Haloferax volcanii is essential for growth on isoleucine but not on other branched-chain amino acidsMarco Sisignano
Goethe University, Biocentre, Institute for Molecular Biosciences, Max von Laue Str 9, D 60438 Frankfurt, Germany
Microbiology 156:521-9. 2010..Taken together, the results indicate that OADHC1 is a specialized BCDHC that uses only one (or maximally two) of the three branched-chain 2-oxoacids, in contrast to BCDHCs from other species... - Small RNAs in haloarchaea: identification, differential expression and biological functionJulia Straub
Institute of Molecular Biosciences, Goethe University, Frankfurt, Germany
RNA Biol 6:281-92. 2009..Taken together, we showed that haloarchaea encode sRNAs, some of which are differentially expressed and which have the potential to fulfil important biological functions in vivo... 
Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivoMariam Brenneis
Goethe University, Institute for Molecular Biosciences, Frankfurt, Germany
PLoS ONE 4:e4484. 2009..The current results indicate that 3'-UTR-dependent translational control had already evolved before capping and polyadenylation of transcripts were invented, which are essential for circularization of transcripts in eukaryotes...- Experimental characterization of Cis-acting elements important for translation and transcription in halophilic archaeaMariam Brenneis
Institute for Molecular Biosciences, Goethe University, Frankfurt, Germany
PLoS Genet 3:e229. 2007..Taken together, either a scanning mechanism similar to the mechanism of translation initiation operating in eukaryotes or a novel mechanism must operate on most leadered haloarchaeal transcripts... - A novel mechanism for translation initiation operates in haloarchaeaOliver Hering
Goethe University, Department of Biosciences, Biocentre, Institute for Molecular Biosciences, Max von Laue Str 9, D 60438 Frankfurt, Germany
Mol Microbiol 71:1451-63. 2009..Taken together, operation of a scanning mechanism was excluded and the results indicate that a novel mechanism for translation initiation operates at least in haloarchaea... - Characterization of a Haloferax volcanii member of the enolase superfamily: deletion mutant construction, expression analysis, and transcriptome comparisonMichael Dambeck
Biocentre, Institute for Molecular Biosciences, Goethe University, Max von Laue Str 9, 60438 Frankfurt, Germany
Arch Microbiol 190:341-53. 2008..Taken together, the results indicate that IftA might have a dual function, i.e., transiently after transition to fresh medium and permanently during growth in glucose medium... - Functional genomic and advanced genetic studies reveal novel insights into the metabolism, regulation, and biology of Haloferax volcaniiJörg Soppa
Institute for Molecular Biosciences, Goethe University Frankfurt, Biocentre, Frankfurt, Germany
Archaea 2011:602408. 2011..volcanii a bona fide archaeal model species, which has enabled the generation of important results in recent years and will most likely generate further breakthroughs in the future... - Transcriptome changes and cAMP oscillations in an archaeal cell cycleAnke Baumann
Goethe University, Institute for Molecular Biosciences, Biocentre, Frankfurt, Germany
BMC Cell Biol 8:21. 2007..Similarly, oscillations of small signaling molecules have been identified in very few eukaryotic species, but not in any prokaryote... - Ploidy and gene conversion in ArchaeaJörg Soppa
Goethe University, Biocentre, Institute for Molecular Biosciences, Max von Laue Strasse 9, D 60438 Frankfurt, Germany
Biochem Soc Trans 39:150-4. 2011..In both cases, the genomes were rapidly equalized in the absence of selection, showing that gene conversion operates at least in halophilic and methanogenic Euryarchaea... - Microarray analysis in the archaeon Halobacterium salinarum strain R1Jens Twellmeyer
Max Planck Institute of Biochemistry, Membrane Biochemistry, Martinsried, Germany
PLoS ONE 2:e1064. 2007..The tool of choice to record transcriptional profiles is the DNA microarray technique. However, the technique is still rarely used for transcriptome analysis in archaea... - Three 2-oxoacid dehydrogenase operons in Haloferax volcanii: expression, deletion mutants and evolutionJan van Ooyen
Goethe University, Biocentre, Institute for Molecular Biosciences, Max von Laue Str 9, D 60438 Frankfurt, Germany
Microbiology 153:3303-13. 2007..This view is underscored by a phylogenetic tree of 33 archaeal and bacterial OADHCs... - Quantification of ploidy in proteobacteria revealed the existence of monoploid, (mero-)oligoploid and polyploid speciesVito Pecoraro
Biocentre, Institute for Molecular Biosciences, Goethe University, Frankfurt, Germany
PLoS ONE 6:e16392. 2011..The ploidy level is not conserved within the groups of proteobacteria, and there are no obvious correlations between the ploidy levels with other parameters like genome size, optimal growth temperature or mode of life... - Optimized generation of vectors for the construction of Haloferax volcanii deletion mutantsM Hammelmann
Institute for Molecular Biosciences, Biocentre, Goethe University, D 60438 Frankfurt, Germany
J Microbiol Methods 75:201-4. 2008..Taken together, the optimized procedure omits one cloning step and enhances both speed and efficiency of deletion vector construction considerably... - Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaeaChristian Lange
Institute for Molecular Biosciences, Johann Wolfgang Goethe University, Max von Laue Strasse 9, 60438 Frankfurt a, M, Germany
BMC Genomics 8:415. 2007..Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available... - Ploidy in cyanobacteriaMarco Griese
Institute for Molecular Biosciences, Biocentre, Goethe University, Frankfurt, Germany
FEMS Microbiol Lett 323:124-31. 2011..A compilation of the ploidy levels of all investigated cyanobacterial species gives an overview of the genome copy number distribution and shows that monoploid, oligoploid, and polyploid cyanobacteria exist... - Genome copy numbers and gene conversion in methanogenic archaeaCatherina Hildenbrand
Goethe University, Biocentre, Institute for Molecular Biosciences, Max von Laue Str 9, D 60438 Frankfurt, Germany
J Bacteriol 193:734-43. 2011..maripaludis took place probably via a gene conversion mechanism. In addition, it was shown that the velocity of this phenomenon is inversely correlated to the strength of selection...