Thomas Langer

Summary

Affiliation: Frankfurt am Main
Country: Germany

Publications

  1. ncbi Intracellular localization of the 90 kDA heat shock protein (HSP90alpha) determined by expression of a EGFP-HSP90alpha-fusion protein in unstressed and heat stressed 3T3 cells
    Thomas Langer
    Institut für Biochemie und Biophysikalische Chemie der Johann Wolfgang Goethe Universität Frankfurt am Main, Marie Curie Str 9, 60439 Frankfurt am Main, Germany
    Cell Biol Int 27:47-52. 2003
  2. ncbi Folding and activity of cAMP-dependent protein kinase mutants
    Thomas Langer
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    FEBS Lett 579:4049-54. 2005
  3. ncbi NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: application to the catalytic subunit of cAMP-dependent protein kinase
    Thomas Langer
    Johann Wolfgang Goethe Universitat Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 5:1508-16. 2004
  4. ncbi How much NMR data is required to determine a protein-ligand complex structure?
    Ulrich Schieborr
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1891-8. 2005
  5. ncbi NMR characterization of kinase p38 dynamics in free and ligand-bound forms
    Martin Vogtherr
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Germany
    Angew Chem Int Ed Engl 45:993-7. 2006
  6. ncbi NMR backbone assignment of the N-terminal domain of human HSP90
    Doris M Jacobs
    Institut für Organische Chemie und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Str 7, Building N160, Room 314, D 60439, Frankfurt am Main, Germany
    J Biomol NMR 36:52. 2006
  7. doi The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy
    Sridhar Sreeramulu
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt am Main D 60438, Germany
    J Biol Chem 284:3885-96. 2009
  8. ncbi Using wavelet de-noised spectra in NMR screening
    Nikola Trbovic
    Center for Biomolecular Magnetic Resonance BMRZ, Institute of Biophysical Chemistry, J W Goethe University, Marie Curie Str 9, 60439 Frankfurt, Germany
    J Magn Reson 173:280-7. 2005
  9. pmc A novel function for the 90 kDa heat-shock protein (Hsp90): facilitating nuclear export of 60 S ribosomal subunits
    Harald Schlatter
    Institut fur Biochemie, Johann Wolfgang Goethe Universität Frankfurt am Main, Marie Curie Str 9, 60439 Frankfurt am Main, Germany
    Biochem J 362:675-84. 2002
  10. ncbi 1H, 13C and 15N backbone resonance assignment of the integrin alpha2 I-domain
    Bettina Elshorst
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    J Biomol NMR 27:191-2. 2003

Collaborators

  • Harald Schwalbe
  • Doris M Jacobs
  • Swen Hoelder
  • C Roy D Lancaster
  • Isabelle Landrieu
  • Stefan Geimer
  • G Lippens
  • H Waldmann
  • Ulrich Günther
  • W Neupert
  • Krishna Saxena
  • Martin Vogtherr
  • Ulrich Schieborr
  • Susanne Grimme
  • Barbara Pescatore
  • Marco Betz
  • Sridhar Sreeramulu
  • Martin Graef
  • Elena I Rugarli
  • Takashi Tatsuta
  • Bettina Elshorst
  • Mirko Koppen
  • Mafalda Escobar-Henriques
  • Stéphane Duvezin-Caubet
  • Christian Richter
  • Mark Dürr
  • Benedikt Westermann
  • Michael Manger
  • Nikola Trbovic
  • Daniel Korbel
  • Dominik Galluhn
  • Harald Schlatter
  • Hendrik R A Jonker
  • Giorgio Casari
  • Björn Friedrichs
  • Ravi Jagasia
  • Steffen Augustin
  • Johannes Wagener
  • Metodi D Metodiev
  • Andreas S Reichert
  • Michael Zick
  • Mark Nolden
  • Lars Israel
  • Axel Imhof
  • Andrea Bernacchia
  • Georgeta Seewald
  • Michel Robin
  • Sandra Merz
  • K Ulrich Wendt
  • Laure Delarbre
  • Holger Berk
  • Jens Peter von Kries
  • Klaus Fiebig
  • Kirstin Model
  • Michael Scheck
  • Jörg Rademann
  • Bettina Elshort
  • Felician Dancea
  • Jitendra Kumar
  • Alois Fürstner
  • Heino Prinz
  • Stephanie Wurth
  • Michael Kaser
  • Susann Rosmus
  • Hugo Fasold
  • Marie Luise Onneken

Detail Information

Publications29

  1. ncbi Intracellular localization of the 90 kDA heat shock protein (HSP90alpha) determined by expression of a EGFP-HSP90alpha-fusion protein in unstressed and heat stressed 3T3 cells
    Thomas Langer
    Institut für Biochemie und Biophysikalische Chemie der Johann Wolfgang Goethe Universität Frankfurt am Main, Marie Curie Str 9, 60439 Frankfurt am Main, Germany
    Cell Biol Int 27:47-52. 2003
    ..As the most remarkable finding under these conditions, an association of EGFP-Hsp90alpha with the nuclear membrane became visible...
  2. ncbi Folding and activity of cAMP-dependent protein kinase mutants
    Thomas Langer
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    FEBS Lett 579:4049-54. 2005
    ..These proteins can be expressed in high yields but have reduced activity compared to the wild-type. Proper folding of all proteins was analyzed by 2D 1H, 15N-TROSY NMR experiments...
  3. ncbi NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: application to the catalytic subunit of cAMP-dependent protein kinase
    Thomas Langer
    Johann Wolfgang Goethe Universitat Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 5:1508-16. 2004
    ..Furthermore, structural conformational changes were observed by comparison of backbone amide shifts obtained by 2D (1)H,(15)N TROSY of an inactive Thr197Ala mutant with the wild-type enzyme...
  4. ncbi How much NMR data is required to determine a protein-ligand complex structure?
    Ulrich Schieborr
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1891-8. 2005
    ....
  5. ncbi NMR characterization of kinase p38 dynamics in free and ligand-bound forms
    Martin Vogtherr
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Germany
    Angew Chem Int Ed Engl 45:993-7. 2006
  6. ncbi NMR backbone assignment of the N-terminal domain of human HSP90
    Doris M Jacobs
    Institut für Organische Chemie und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Str 7, Building N160, Room 314, D 60439, Frankfurt am Main, Germany
    J Biomol NMR 36:52. 2006
  7. doi The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy
    Sridhar Sreeramulu
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt am Main D 60438, Germany
    J Biol Chem 284:3885-96. 2009
    ..NMR and mutagenesis experiments reveal Leu-205 in Cdc37 as a key residue enabling complex formation. These findings can be very useful in the development of small molecule inhibitors against cancer...
  8. ncbi Using wavelet de-noised spectra in NMR screening
    Nikola Trbovic
    Center for Biomolecular Magnetic Resonance BMRZ, Institute of Biophysical Chemistry, J W Goethe University, Marie Curie Str 9, 60439 Frankfurt, Germany
    J Magn Reson 173:280-7. 2005
    ..The combination of wavelet de-noising and PCA is most efficient when PCA is applied to wavelet coefficients. This new algorithm yields good clustering and can be applied to series of one- or two-dimensional spectra...
  9. pmc A novel function for the 90 kDa heat-shock protein (Hsp90): facilitating nuclear export of 60 S ribosomal subunits
    Harald Schlatter
    Institut fur Biochemie, Johann Wolfgang Goethe Universität Frankfurt am Main, Marie Curie Str 9, 60439 Frankfurt am Main, Germany
    Biochem J 362:675-84. 2002
    ..These findings suggest that Hsp90 facilitates the nuclear export of 60 S ribosomal subunits, probably by chaperoning protein interactions during the export process...
  10. ncbi 1H, 13C and 15N backbone resonance assignment of the integrin alpha2 I-domain
    Bettina Elshorst
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    J Biomol NMR 27:191-2. 2003
  11. ncbi Backbone NMR assignment of the C-terminal haemopexin-like domain (HPLD) of human matrix metalloproteinase MMP-13
    Doris M Jacobs
    J Biomol NMR 32:337. 2005
  12. ncbi 1H, 13C and 15N backbone resonance assignment of the peptidyl-prolyl cis-trans isomerase Pin1
    Doris M Jacobs
    J Biomol NMR 23:163-4. 2002
  13. ncbi NMR analysis of a Tau phosphorylation pattern
    Isabelle Landrieu
    CNRS UMR 8525, Institut Pasteur de Lille, 59019 Lille Cedex, France
    J Am Chem Soc 128:3575-83. 2006
    ..We finally demonstrate that the NMR approach can equally be used to evaluate potential kinase inhibitors in a straightforward manner...
  14. ncbi Discovery of Mycobacterium tuberculosis protein tyrosine phosphatase A (MptpA) inhibitors based on natural products and a fragment-based approach
    Michael Manger
    Max Planck Institut fur molekulare Physiologie, Abteilung Chemische Biologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Chembiochem 6:1749-53. 2005
  15. pmc OPA1 processing reconstituted in yeast depends on the subunit composition of the m-AAA protease in mitochondria
    Stéphane Duvezin-Caubet
    Institute for Physiological Chemistry, Ludwig Maximilians University, 81377 Munich, Germany
    Mol Biol Cell 18:3582-90. 2007
    ....
  16. pmc Substrate recognition by AAA+ ATPases: distinct substrate binding modes in ATP-dependent protease Yme1 of the mitochondrial intermembrane space
    Martin Graef
    Institut fur Genetik, Universitat zu Koln, Zulpicher Strasse 47, 50674 Koln, Germany
    Mol Cell Biol 27:2476-85. 2007
    ..Our findings therefore reveal the existence of alternative substrate recognition pathways within AAA proteases and shed new light on molecular mechanisms ensuring the specificity of proteolysis by energy-dependent proteases...
  17. pmc m-AAA protease-driven membrane dislocation allows intramembrane cleavage by rhomboid in mitochondria
    Takashi Tatsuta
    Institute for Genetics and Center for Molecular Medicine CMMC, University of Cologne, Cologne, Germany
    EMBO J 26:325-35. 2007
    ..These findings reveal for the first time a non-proteolytic function of the m-AAA protease during mitochondrial biogenesis and rationalise the requirement of a preceding step for intramembrane cleavage by rhomboid...
  18. pmc Variable and tissue-specific subunit composition of mitochondrial m-AAA protease complexes linked to hereditary spastic paraplegia
    Mirko Koppen
    Institut fur Genetik, Universitat zu Koln, Zulpicher Strasse, 0674 Köln, Germany
    Mol Cell Biol 27:758-67. 2007
    ..We therefore propose that the formation of m-AAA proteases with altered substrate specificities leads to axonal degeneration in HSP...
  19. ncbi Reversible assembly of the ATP-binding cassette transporter Mdl1 with the F1F0-ATP synthase in mitochondria
    Dominik Galluhn
    Institut fur Genetik, Universitat zu Koln, 50674 Koln, Germany
    J Biol Chem 279:38338-45. 2004
    ..These results are consistent with an activation of Mdl1 upon dissociation from the ATP synthase and suggest a link of peptide export from mitochondria to the activity of the F(1)F(0)-ATP synthase and the cellular energy metabolism...
  20. pmc Nonredundant roles of mitochondria-associated F-box proteins Mfb1 and Mdm30 in maintenance of mitochondrial morphology in yeast
    Mark Dürr
    Institut fur Zellbiologie, Universitat Bayreuth, 95440 Bayreuth, Germany
    Mol Biol Cell 17:3745-55. 2006
    ..We propose that mitochondria-associated F-box proteins Mfb1 and Mdm30 are key components of a complex machinery that regulates mitochondrial dynamics throughout yeast's entire life cycle...
  21. pmc Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzo1
    Mafalda Escobar-Henriques
    Institute of Genetics and Center for Molecular Medicine, University of Cologne, D 50923 Cologne, Germany
    J Cell Biol 173:645-50. 2006
    ....
  22. ncbi Translating m-AAA protease function in mitochondria to hereditary spastic paraplegia
    Elena I Rugarli
    Istituto Nazionale Neurologico C Besta, Division of Biochemistry and Genetics, 20126 Milan, Italy
    Trends Mol Med 12:262-9. 2006
    ..Here, we will discuss implications of the dual role of the m-AAA protease in protein activation and degradation for mitochondrial dysfunction and axonal degeneration...
  23. ncbi NMR backbone assignment of the mitogen-activated protein (MAP) kinase p38
    Martin Vogtherr
    J Biomol NMR 32:175. 2005
  24. ncbi Substrate specific consequences of central pore mutations in the i-AAA protease Yme1 on substrate engagement
    Martin Graef
    Institute for Genetics and Center for Molecular Medicine, CMMC, University of Cologne, Cologne, Germany
    J Struct Biol 156:101-8. 2006
    ..Our findings therefore suggest an essential function of the central pore loop for the ATP-dependent translocation of membrane proteins into a proteolytic cavity formed by AAA proteases...
  25. ncbi 1H, 13C and 15N backbone resonance assignment of the Hsp90 binding domain of human Cdc37
    Sridhar Sreeramulu
    J Biomol NMR 32:262. 2005
  26. ncbi Backbone NMR assignment of the low-molecular-weight protein tyrosine phosphatase (MPtpA) from Mycobacterium tuberculosis
    Krishna Saxena
    J Biomol NMR 33:136. 2005
  27. ncbi Backbone NMR assignment of the human E2 ubiquitin conjugating enzyme UbcH5alpha (F72K,F82S) double mutant
    Krishna Saxena
    J Biomol NMR 32:338. 2005
  28. pmc Membrane protein turnover by the m-AAA protease in mitochondria depends on the transmembrane domains of its subunits
    Daniel Korbel
    Institut fur Genetik, Universitat zu Koln, Zulpicher Strasse 47, 50674 Koln, Germany
    EMBO Rep 5:698-703. 2004
    ..We therefore propose that transmembrane segments of m-AAA protease subunits have a direct role in the dislocation of membrane-embedded substrates...
  29. pmc Formation of membrane-bound ring complexes by prohibitins in mitochondria
    Takashi Tatsuta
    Institut für Genetik and Zentrum für Molekulare Medizin, Universitat zu Koln, 50674 Koln, Germany
    Mol Biol Cell 16:248-59. 2005
    ..Implications of these findings for proposed cellular activities of prohibitins are discussed...