Genomes and Genes
K D Entian
Affiliation: Frankfurt am Main
- Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approachK D Entian
Institut fur Mikrobiologie, Universitat Frankfurt, Frankfurt Main, Germany
Mol Gen Genet 262:683-702. 1999..Surprisingly, for seven genes, additional, unexpected phenotypes were found in our tests. This suggests that the type of analysis presented here provides a more complete description of gene function...
- The succinate/fumarate transporter Acr1p of Saccharomyces cerevisiae is part of the gluconeogenic pathway and its expression is regulated by Cat8pN Bojunga
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat, Frankfurt Main, Germany
Mol Gen Genet 260:453-61. 1998....
- The mitochondrial cytochrome c peroxidase Ccp1 of Saccharomyces cerevisiae is involved in conveying an oxidative stress signal to the transcription factor Pos9 (Skn7)C Charizanis
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat Frankfurt, Biozentrum, Frankfurt am Main, Germany
Mol Gen Genet 262:437-47. 1999..The Ccp1W191F mutant was still capable of activating the Pos9 transcriptional activation domain, suggesting that the signalling function of Ccp1 is independent of electron flux rates...
- Carbon source-dependent phosphorylation of hexokinase PII and its role in the glucose-signaling response in yeastF Randez-Gil
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat Frankfurt, Frankfurt am Main, Germany
Mol Cell Biol 18:2940-8. 1998..Although we cannot rule out a defect in the metabolic state of the cell as the origin of these phenomena, our results suggest that the phosphorylation of hexokinase is essential in vivo for glucose signal transduction...
- Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stressH Juhnke
Institut fur Mikrobiologie, der Johann Wolfgang Goethe Universität Frankfurt, Biozentrum Niederursel, Frankfurt am Main, Germany
Mol Gen Genet 252:456-64. 1996..Interestingly, all mutants tested (glucose 6-phosphate dehydrogenase, gluconate 6-phosphate dehydrogenase, ribulose 5-phosphate epimerase, transketolase, transaldolase) are sensitive to hydrogen peroxide...
- Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB proteinG Engelke
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat, Frankfurt Main, Germany
Appl Environ Microbiol 58:3730-43. 1992..The NisB protein was tightly associated with the vesicle fraction and was released by sodium dodecyl sulfate treatment only. These results suggest that NisB is membrane associated and that nisin biosynthesis occurs at the cell membrane...
- Characterization of null mutants of the glyoxylate cycle and gluconeogenic enzymes in S. cerevisiae through metabolic network modeling verified by chemostat cultivationI Stückrath
Institut fur Mikrobiologie, J W Goethe Universität Frankfurt, Germany
Biotechnol Bioeng 77:61-72. 2002..The growth of the fructose- 1,6-bisphosphatase deletion mutant was anticipated less accurate, but the tendency was modeled correctly...
- Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8pF Randez-Gil
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat Frankfurt, Biozentrum, Niederursel, Frankfurt am Main, Germany
Mol Cell Biol 17:2502-10. 1997..These results confirm our current model that glucose derepression of gluconeogenic genes needs Cat8p phosphorylation and additionally show that a still unknown transcriptional activator is also involved...
- Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd(2+) ion resistanceI M Solovieva
Molecular and Radiation Biophysics Division, St Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, PNPI, Gatchina, 188350, St Petersburg, Russia
FEMS Microbiol Lett 208:105-9. 2002..Northern hybridization analysis showed that cadmium induces the synthesis of a 2.2-kb cadA transcript. These results indicate that cadA is the chromosomal determinant to cadmium resistance in B. subtilis...
- Mutants of Saccharomyces cerevisiae sensitive to oxidative and osmotic stressB Krems
Institut fur Mikrobiologie, Frankfurt Main, Germany
Curr Genet 27:427-34. 1995..abstract truncated at 250 words)..
- New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporterM Rose
Johann Wolfgang Goethe Universitat Frankfurt, Institut fur Mikrobiologie, Germany
Microbiology 142:3097-101. 1996..The prkA gene, which was mapped at 169 degrees, was not present on the respective fragment. Sequence comparison actually showed that prkA is located close to 70 degrees on the B. subtilis genome...
- Genetic analysis of serine biosynthesis and glucose repression in yeastK Melcher
Institut fur Mikrobiologie, J W Goethe Universitat, Frankfurt Main, Federal Republic of Germany
Curr Genet 21:295-300. 1992..These results indicate that transcription of glucose-repressible gluconeogenic enzymes is mainly dependent on positive regulatory elements...
- Structure of the Bacillus subtilis peptide antibiotic subtilosin A determined by 1H-NMR and matrix assisted laser desorption/ionization time-of-flight mass spectrometryR Marx
J Protein Chem 20:501-6. 2001....
- Comparative genomics of Listeria speciesP Glaser
, , Service d'Informatique Scientifique, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France
Science 294:849-52. 2001..The presence of 270 L. monocytogenes and 149 L. innocua strain-specific genes (clustered in 100 and 63 islets, respectively) suggests that virulence in Listeria results from multiple gene acquisition and deletion events...
- The essential protein fap7 is involved in the oxidative stress response of Saccharomyces cerevisiaeH Juhnke
Institut für Mikrobiologie der Johann Wolfgang Goethe Universität Frankfurt, Biozentrum, Niederursel, Marie Curie Strasse 9, D 60439 Frankfurt am Main, Germany
Mol Microbiol 35:936-48. 2000..Fluorescence microscopy of Fap7-GFP fusion proteins indicated a nuclear localization of the Fap7 protein. Our data suggest that Fap7 is a nuclear factor important for Pos9-dependent target gene transcription upon oxidative stress...
- Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeastD Niederacher
Institut fur Mikrobiologie, Johann Wolfgang Goethe Universitat Frankfurt, Federal Republic of Germany
Eur J Biochem 200:311-9. 1991..There are two strongly acidic regions within this part of the protein, their possible importance is discussed...
- Isolation and primary structure of the gene encoding fructose-1,6-bisphosphatase from Saccharomyces cerevisiaeK D Entian
Medizinisch Naturwissenschaftliches Forschungszentrum, Universitat Tubingen, FRG
FEBS Lett 236:195-200. 1988..The cloned promoter size was 318 bp and allowed only low level expression of the gene. This indicates a positive activation site (UAS) upstream of the cloned DNA fragment...
- PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replicationM A Petit
Laboratoire de Genetique Microbienne, INRA, Jouy en Josas, France
Mol Microbiol 29:261-73. 1998..Furthermore, it conferred a Rep-phenotype on E. coli. Altogether, these results show that PcrA is an helicase used for plasmid rolling-circle replication and suggest that it is also involved in UV repair...
- CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiaeD Hedges
Institut für Mikrobiologie der Johann Wolfgang Goethe Universität Frankfurt, Germany
Mol Cell Biol 15:1915-22. 1995..The observation that multicopy expression of CAT8 reverses the inability of cat1 and cat3 mutants to grow on ethanol indicates that Cat8p might be the substrate of the Cat1p/Cat3p protein kinase...
- CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiaeM Proft
Institut für Mikrobiologie der Johann Wolfgang Goethe Universität Frankfurt, Frankfurt am Main, Germany
EMBO J 14:6116-26. 1995....
- Molecular analysis of the yeast SER1 gene encoding 3-phosphoserine aminotransferase: regulation by general control and serine repressionK Melcher
Institute for Microbiology, University of Frankfurt, Germany
Curr Genet 27:501-8. 1995..Furthermore, there is evidence for an additional regulation by serine end product repression...
- Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolismD Ochs
Botanisches Institut, Mikrobiologie, Universitat Tubingen, Germany
J Bacteriol 174:298-302. 1992..According to the DNA sequence, the cyclase contained 627 amino acids with a molecular mass of 69,473 Da. A high percentage of the amino acids were basic. No significant similarity to existing sequenced proteins was found...
- The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistanceB Krems
Institute for Microbiology, Johann Wolfgang Goethe University, Frankfurt, Germany
Curr Genet 29:327-34. 1996..Two-hybrid investigations also suggest an oligomerization of the Pos9 protein. Our results indicate that a two-component system is involved in the oxidative-stress response of yeast...
- Conservation of microstructure between a sequenced region of the genome of rice and multiple segments of the genome of Arabidopsis thalianaK Mayer
National Research Center for Environment and Health, Institute for Bioinformatics, Munich Information Centre for Protein Sequences, 85764 Neuherberg, Germany
Genome Res 11:1167-74. 2001....
- Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeastT Schule
Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart and Institut für Mikrobiologie, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strabetae 9, 60439 Frankfurt, Germany
EMBO J 19:2161-7. 2000..Like FBPase, Ubc8p is found in the cytoplasmic fraction of the cell. We demonstrate cytoplasmic degradation of FBPase...
- Yeast Clk-1 homologue (Coq7/Cat5) is a mitochondrial protein in coenzyme Q synthesisT Jonassen
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
J Biol Chem 273:3351-7. 1998..elegans clk-1 mutants may relate to changes in the amount of ubiquinone, an essential electron transport component and a lipid soluble antioxidant...
- Extragenic suppressors of yeast glucose derepression mutants leading to constitutive synthesis of several glucose-repressible enzymesH J Schüller
Institute for Microbiology, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Federal Republic of Germany
J Bacteriol 173:2045-52. 1991..Allelism tests and tetrad analysis clearly proved that the cat4 complementation group is a new class of mutant alleles affecting carbon source-dependent gene expression...
- New genes involved in carbon catabolite repression and derepression in the yeast Saccharomyces cerevisiaeK D Entian
J Bacteriol 151:1123-8. 1982..cat3 mutants had the same phenotypic properties as cat1 mutants. This showed that carbon metabolism in yeast cells is under a very complex and ramified control of repressing and derepressing genes, which are interdependent...