Volker Dötsch

Summary

Affiliation: Frankfurt am Main
Country: Germany

Publications

  1. ncbi request reprint How to create a specific recognition for an unspecific interaction
    Volker Dötsch
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt Main, Germany
    Structure 19:601-2. 2011
  2. pmc The C-terminus of p63 contains multiple regulatory elements with different functions
    W E Straub
    Institute of Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
    Cell Death Dis 1:e5. 2010
  3. doi request reprint Investigation of proteins in living bacteria with in-cell NMR experiments
    Volker Dötsch
    Institute of Biophysical Chemistry, University of Frankfurt, Max von Laue Str 9, 60438, Frankfurt, Germany
    Top Curr Chem 273:203-14. 2008
  4. pmc DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramer
    Gregor B Deutsch
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
    Cell 144:566-76. 2011
  5. ncbi request reprint Conformational switches modulate protein interactions in peptide antibiotic synthetases
    Alexander Koglin
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance BMRZ, J W Goethe University of Frankfurt, Marie Curie Strasse, D 60439 Frankfurt Main, Germany
    Science 312:273-6. 2006
  6. doi request reprint Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies
    Jochen Stehle
    Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt, Germany
    J Biomol NMR 54:401-13. 2012
  7. doi request reprint Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition
    Laura M Luh
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, 60438, Germany
    J Am Chem Soc 135:13796-803. 2013
  8. doi request reprint Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments
    Davide Proverbio
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
    Biochim Biophys Acta 1828:2182-92. 2013
  9. doi request reprint A universal expression tag for structural and functional studies of proteins
    Vladimir V Rogov
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    Chembiochem 13:959-63. 2012
  10. ncbi request reprint Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues
    Frank Lohr
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Biozentrum N230, 1 OG, Marie Curie Strasse 9, D 60439, Frankfurt, Germany
    J Biomol NMR 32:309-28. 2005

Collaborators

Detail Information

Publications40

  1. ncbi request reprint How to create a specific recognition for an unspecific interaction
    Volker Dötsch
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt Main, Germany
    Structure 19:601-2. 2011
    ..2011) shows how the modification of the lysine side chain is recognized without specific interaction with the peptide...
  2. pmc The C-terminus of p63 contains multiple regulatory elements with different functions
    W E Straub
    Institute of Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
    Cell Death Dis 1:e5. 2010
    ..We therefore propose that the inhibitory function of the C-terminal domain is in part due to direct inhibition of the transcriptional activity of the protein and in part due to indirect inhibition by controlling the concentration of p63...
  3. doi request reprint Investigation of proteins in living bacteria with in-cell NMR experiments
    Volker Dötsch
    Institute of Biophysical Chemistry, University of Frankfurt, Max von Laue Str 9, 60438, Frankfurt, Germany
    Top Curr Chem 273:203-14. 2008
    ..In addition potential applications of thesein-cell NMR experiments and their limitations are discussed...
  4. pmc DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramer
    Gregor B Deutsch
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
    Cell 144:566-76. 2011
    ..Our results demonstrate how TAp63α is inhibited by complex domain-domain interactions that provide the basis for regulating quality control in oocytes...
  5. ncbi request reprint Conformational switches modulate protein interactions in peptide antibiotic synthetases
    Alexander Koglin
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance BMRZ, J W Goethe University of Frankfurt, Marie Curie Strasse, D 60439 Frankfurt Main, Germany
    Science 312:273-6. 2006
    ..We further demonstrate that this conformational diversity is an essential prerequisite for the directed movement of the 4'-PP cofactor and its interaction with externally acting proteins such as thioesterases and 4'-PP transferase...
  6. doi request reprint Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies
    Jochen Stehle
    Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt, Germany
    J Biomol NMR 54:401-13. 2012
    ....
  7. doi request reprint Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition
    Laura M Luh
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, 60438, Germany
    J Am Chem Soc 135:13796-803. 2013
    ..In addition, we demonstrate that commonly used synthetic crowding agents like Ficoll 70 are not suitable to mimic the intracellular environment due to their incapability to simulate biologically important weak attractive interactions. ..
  8. doi request reprint Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments
    Davide Proverbio
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
    Biochim Biophys Acta 1828:2182-92. 2013
    ..High affinity and specific binding of ligands could furthermore be obtained with non-purified receptor samples in crude cell-free reaction mixtures, thus providing new perspectives for fast in vitro screening applications. ..
  9. doi request reprint A universal expression tag for structural and functional studies of proteins
    Vladimir V Rogov
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    Chembiochem 13:959-63. 2012
    ..With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers...
  10. ncbi request reprint Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues
    Frank Lohr
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Biozentrum N230, 1 OG, Marie Curie Strasse 9, D 60439, Frankfurt, Germany
    J Biomol NMR 32:309-28. 2005
    ..The new methods are demonstrated with five different proteins with molecular weights ranging from 11 to 28 kDa...
  11. doi request reprint Fast automated NMR spectroscopy of short-lived biological samples
    Suhas Tikole
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt am Main, Germany
    Chembiochem 13:964-7. 2012
    ....
  12. ncbi request reprint Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR
    Franz Tumulka
    Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max von Laue Str 9, 60438, Frankfurt am Main, Germany
    J Biomol NMR 57:141-54. 2013
    ..NMR spectra with sufficient resolution and homogeneity were finally obtained with a TMD0 derivative only modified by a C-terminal His10-tag and containing a codon optimized AT-rich sequence. ..
  13. ncbi request reprint Structural basis for phosphorylation-triggered autophagic clearance of Salmonella
    Vladimir V Rogov
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max van Laue Str 9, 60438 Frankfurt am Main, Germany
    Biochem J 454:459-66. 2013
    ..Phosphorylation, therefore, acts as a switch for optineurin-based selective autophagy...
  14. doi request reprint Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1
    Alexis Rozenknop
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
    J Mol Biol 410:477-87. 2011
    ..This indicates that different LIRs can interact with autophagy modifiers with unique binding properties...
  15. ncbi request reprint High-resolution insight into G-overhang architecture
    Robert Hänsel
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt Main, Germany
    J Am Chem Soc 135:2816-24. 2013
    ....
  16. doi request reprint Requirements on paramagnetic relaxation enhancement data for membrane protein structure determination by NMR
    Daniel Gottstein
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt am Main, Germany
    Structure 20:1019-27. 2012
    ..These findings are in line with our recent first de novo NMR structure determination of a heptahelical integral membrane protein, proteorhodopsin, that relied extensively on PRE data...
  17. doi request reprint Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase
    Christian Roos
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
    Biochim Biophys Acta 1818:3098-106. 2012
    ..Active MraY protein could only be obtained with ND containing anionic lipids, thus providing new details for the in vitro analysis of this pharmaceutically important protein...
  18. pmc Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment
    Frank Lohr
    Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
    J Biomol NMR 52:197-210. 2012
    ....
  19. pmc A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF
    Vladimir V Rogov
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt Main, Germany
    J Biol Chem 286:18775-83. 2011
    ..Interaction studies showed a weak interaction with an RNA hairpin. However, no interaction could be detected with reagents that are believed to activate the rcs phosphorelay, such as lysozyme, glucose, or Zn(2+) ions...
  20. ncbi request reprint Crystal structure of a PCP/Sfp complex reveals the structural basis for carrier protein posttranslational modification
    Peter Tufar
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt Main, Max von Laue Strasse 9, 60438 Frankfurt, Germany Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt Main, Max von Laue Strasse 15, 60438 Frankfurt, Germany
    Chem Biol 21:552-62. 2014
    ..Comparison with a similar structure of a complex of human proteins suggests that the mode of interaction is highly conserved in all domains of life. ..
  21. doi request reprint Investigation of quadruplex structure under physiological conditions using in-cell NMR
    Robert Hänsel
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt Main, Germany
    Top Curr Chem 330:47-65. 2013
    ..Finally, future perspectives of in-cell NMR spectroscopy for quantitative characterization of G-quadruplexes and NA are discussed...
  22. doi request reprint A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems
    Stefan Haberstock
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J W Goethe University, Frankfurt am Main, Germany
    Protein Expr Purif 82:308-16. 2012
    ..All proteins were finally synthesized in amounts sufficient for further quality optimization and initial crystallization screens...
  23. pmc Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex
    Susanne Stefer
    Institute for Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, D 60325 Frankfurt am Main, Germany
    Science 333:758-62. 2011
    ..Docking to the Get1/2 complex allows for conformational changes in Get3 that are required for TA protein insertion. These data suggest a molecular mechanism for nucleotide-regulated delivery of TA proteins...
  24. doi request reprint Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB
    Kerstin Schmöe
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max von Laue Strasse 9, 60438 Frankfurt Main, Germany
    Structure 19:577-87. 2011
    ..Our results indicate that the effector domain of RcsB, which normally binds to DNA, is recognized by RcsD-ABL, whereas the HPt domain interacts with the phosphoreceiver domain of RcsB...
  25. pmc Tracing the protectors path from the germ line to the genome
    Daniel Coutandin
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Macromolecular Complexes, Goethe University, 60438 Frankfurt Main, Germany
    Proc Natl Acad Sci U S A 107:15318-25. 2010
    ..This suggests that p53 is the most recent evolutionary member of this protein family and predicts a mechanism for p53 activation...
  26. pmc Peak picking NMR spectral data using non-negative matrix factorization
    Suhas Tikole
    Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, and Frankfurt Institute of Advanced Studies, Goethe University Frankfurt am Main, Max von Laue Str, 9, 60438 Frankfurt am Main, Germany
    BMC Bioinformatics 15:46. 2014
    ..For example, NOESY-type spectra have considerable overlaps leading to significant peak-picking intensity errors, which can result in erroneous structural restraints. Precise frequencies are critical for unambiguous resonance assignments...
  27. doi request reprint In-cell solid-state NMR as a tool to study proteins in large complexes
    Sina Reckel
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    Chembiochem 13:534-7. 2012
    ..Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts...
  28. doi request reprint Cell-free expression and assembly of ATP synthase
    Doreen Matthies
    Department of Structural Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, 60438 Frankfurt Main, Germany
    J Mol Biol 413:593-603. 2011
    ..This work establishes the production of highly complex molecular machines in defined environments either as proteomicelles or as proteoliposomes as a new application of CF expression systems...
  29. doi request reprint Modulation of the Rcs-mediated signal transfer by conformational flexibility
    Vladimir V Rogov
    Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
    Biochem Soc Trans 36:1427-32. 2008
    ..Our studies indicate the importance of protein dynamics and conformational exchange for the differential response to the variety of signals perceived by complex regulatory networks...
  30. ncbi request reprint Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteins
    Frank Lohr
    Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany
    J Biomol NMR 37:205-24. 2007
    ..Their performance is compared with that of (Hbeta)Cbeta(CgammaCdelta)Hdelta and (Hbeta)Cbeta(CgammaCdeltaCepsilon)Hepsilon pulse sequences...
  31. doi request reprint Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins
    Friederike Junge
    Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt Main, Max von Laue Str 9, D 60438 Frankfurt Main, Germany
    N Biotechnol 28:262-71. 2011
    ..We give further an overview of the recent advances of cell-free production of membrane proteins for structural and functional analysis...
  32. doi request reprint Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor
    Friederike Junge
    Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt Main, Max von Laue Str 9, Frankfurt Main, Germany
    J Struct Biol 172:94-106. 2010
    ..The presented strategy of cell-free expression protocol development could serve as basic guideline for the production of related receptors in similar systems...
  33. doi request reprint Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy
    Frank Lohr
    Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
    J Biomol NMR 50:167-90. 2011
    ..By applying a four-point Fourier transform, the set of spectra is reversibly interconverted between DIPAP and spin-state representations. Methods are exemplified using proteins of various size...
  34. doi request reprint Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening
    Yi Ma
    School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China
    Protein Expr Purif 86:120-6. 2012
    ..HsGNA1 may be also useful as an antimicrobial and chemotherapeutic target against cancer. Additionally HsGNA1 inhibitors/modulators can possibly be administered with other drugs in the next generation of personalized medicine...
  35. pmc Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes
    Fumiyo Ikeda
    Institute of Biochemistry II, Goethe University Medical School, Frankfurt, Germany
    EMBO J 26:3451-62. 2007
    ..We propose that the ULD is a regulatory component of the TBK1/IKK-i kinases involved in the control of the kinase activation, substrate presentation and downstream signaling pathways...
  36. pmc How to switch a master switch
    Christopher Hein
    is at the Institute of Biophysical Chemistry and the Buchmann Institute of Molecular Life Sciences, Goethe University, Frankfurt, Germany
    elife 2:e01159. 2013
    ..The crystal structure of a nucleotide exchange factor in white blood cells reveals an autoinhibitory mechanism that reinforces the switch-like behaviour of the signalling protein Ras. ..
  37. doi request reprint Solution NMR structure of proteorhodopsin
    Sina Reckel
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt Germany
    Angew Chem Int Ed Engl 50:11942-6. 2011
    ..Restraints from residual dipolar couplings improved the structural accuracy...
  38. pmc Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase
    Alena Busche
    Institute of Biophysical Chemistry, Goethe University Frankfurt and Center for Biomolecular Magnetic Resonance, Frankfurt am Main, Germany
    ACS Chem Biol 7:378-86. 2012
    ..Since the CurA Hal accepts substrate only when presented by one of the ACPs within the ACP(I)-ACP(II)-ACP(III) tridomain, our data provide insight into the specificity of the chlorination reaction...
  39. pmc The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
    Robert Hänsel
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max von Laue Str 9, 60438 Frankfurt Main, Germany
    Nucleic Acids Res 39:5768-75. 2011
    ..laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic...
  40. pmc Structural investigation of the C-terminal catalytic fragment of presenilin 1
    Solmaz Sobhanifar
    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Macromolecular Complexes, Goethe University, 60438 Frankfurt Main, Germany
    Proc Natl Acad Sci U S A 107:9644-9. 2010
    ..These include a putative half-membrane-spanning helix N-terminally harboring the catalytic aspartate, a severely kinked helical structure toward the C terminus as well as a soluble helix in the assumed-to-be unstructured N-terminal loop...