Research Topics
| Volker DötschSummaryAffiliation: Frankfurt am Main Country: Germany Publications
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Detail Information
Publications
Investigation of proteins in living bacteria with in-cell NMR experimentsVolker Dötsch
Institute of Biophysical Chemistry, University of Frankfurt, Max von Laue Str 9, 60438, Frankfurt, Germany
Top Curr Chem 273:203-14. 2008..In addition potential applications of thesein-cell NMR experiments and their limitations are discussed...
How to create a specific recognition for an unspecific interactionVolker Dötsch
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt Main, Germany
Structure 19:601-2. 2011..2011) shows how the modification of the lysine side chain is recognized without specific interaction with the peptide...
DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramerGregor B Deutsch
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
Cell 144:566-76. 2011..Our results demonstrate how TAp63α is inhibited by complex domain-domain interactions that provide the basis for regulating quality control in oocytes...- A universal expression tag for structural and functional studies of proteinsVladimir V Rogov
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
Chembiochem 13:959-63. 2012..With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers... - Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residuesFrank Lohr
Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Biozentrum N230, 1 OG, Marie Curie Strasse 9, D 60439, Frankfurt, Germany
J Biomol NMR 32:309-28. 2005..The new methods are demonstrated with five different proteins with molecular weights ranging from 11 to 28 kDa... - Fast automated NMR spectroscopy of short-lived biological samplesSuhas Tikole
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt am Main, Germany
Chembiochem 13:964-7. 2012.... - Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopiesJochen Stehle
Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt, Germany
J Biomol NMR 54:401-13. 2012.... - Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1Alexis Rozenknop
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
J Mol Biol 410:477-87. 2011..This indicates that different LIRs can interact with autophagy modifiers with unique binding properties... - Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocaseChristian Roos
Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
Biochim Biophys Acta 1818:3098-106. 2012..Active MraY protein could only be obtained with ND containing anionic lipids, thus providing new details for the in vitro analysis of this pharmaceutically important protein... - Requirements on paramagnetic relaxation enhancement data for membrane protein structure determination by NMRDaniel Gottstein
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt am Main, Germany
Structure 20:1019-27. 2012..These findings are in line with our recent first de novo NMR structure determination of a heptahelical integral membrane protein, proteorhodopsin, that relied extensively on PRE data... - Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignmentFrank Lohr
Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
J Biomol NMR 52:197-210. 2012.... - A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSFVladimir V Rogov
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt Main, Germany
J Biol Chem 286:18775-83. 2011..Interaction studies showed a weak interaction with an RNA hairpin. However, no interaction could be detected with reagents that are believed to activate the rcs phosphorelay, such as lysozyme, glucose, or Zn(2+) ions... - A systematic approach to increase the efficiency of membrane protein production in cell-free expression systemsStefan Haberstock
Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J W Goethe University, Frankfurt am Main, Germany
Protein Expr Purif 82:308-16. 2012..All proteins were finally synthesized in amounts sufficient for further quality optimization and initial crystallization screens... - Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complexSusanne Stefer
Institute for Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, D 60325 Frankfurt am Main, Germany
Science 333:758-62. 2011..Docking to the Get1/2 complex allows for conformational changes in Get3 that are required for TA protein insertion. These data suggest a molecular mechanism for nucleotide-regulated delivery of TA proteins... - Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsBKerstin Schmöe
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max von Laue Strasse 9, 60438 Frankfurt Main, Germany
Structure 19:577-87. 2011..Our results indicate that the effector domain of RcsB, which normally binds to DNA, is recognized by RcsD-ABL, whereas the HPt domain interacts with the phosphoreceiver domain of RcsB... - Cell-free expression and assembly of ATP synthaseDoreen Matthies
Department of Structural Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, 60438 Frankfurt Main, Germany
J Mol Biol 413:593-603. 2011..This work establishes the production of highly complex molecular machines in defined environments either as proteomicelles or as proteoliposomes as a new application of CF expression systems... - In-cell solid-state NMR as a tool to study proteins in large complexesSina Reckel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
Chembiochem 13:534-7. 2012..Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts... - Modulation of the Rcs-mediated signal transfer by conformational flexibilityVladimir V Rogov
Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt, Germany
Biochem Soc Trans 36:1427-32. 2008..Our studies indicate the importance of protein dynamics and conformational exchange for the differential response to the variety of signals perceived by complex regulatory networks... - Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteinsFrank Lohr
Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany
J Biomol NMR 37:205-24. 2007..Their performance is compared with that of (Hbeta)Cbeta(CgammaCdelta)Hdelta and (Hbeta)Cbeta(CgammaCdeltaCepsilon)Hepsilon pulse sequences... - Investigation of quadruplex structure under physiological conditions using in-cell NMRRobert Hänsel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt Main, Germany
Top Curr Chem 330:47-65. 2013..Finally, future perspectives of in-cell NMR spectroscopy for quantitative characterization of G-quadruplexes and NA are discussed... - High-resolution insight into G-overhang architectureRobert Hänsel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt Main, Germany
J Am Chem Soc 135:2816-24. 2013.... - Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteinsFriederike Junge
Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt Main, Max von Laue Str 9, D 60438 Frankfurt Main, Germany
N Biotechnol 28:262-71. 2011..We give further an overview of the recent advances of cell-free production of membrane proteins for structural and functional analysis... - Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screeningYi Ma
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China
Protein Expr Purif 86:120-6. 2012..HsGNA1 may be also useful as an antimicrobial and chemotherapeutic target against cancer. Additionally HsGNA1 inhibitors/modulators can possibly be administered with other drugs in the next generation of personalized medicine... - Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptorFriederike Junge
Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt Main, Max von Laue Str 9, Frankfurt Main, Germany
J Struct Biol 172:94-106. 2010..The presented strategy of cell-free expression protocol development could serve as basic guideline for the production of related receptors in similar systems... - Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopyFrank Lohr
Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
J Biomol NMR 50:167-90. 2011..By applying a four-point Fourier transform, the set of spectra is reversibly interconverted between DIPAP and spin-state representations. Methods are exemplified using proteins of various size... - Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genesFumiyo Ikeda
Institute of Biochemistry II, Goethe University Medical School, Frankfurt, Germany
EMBO J 26:3451-62. 2007..We propose that the ULD is a regulatory component of the TBK1/IKK-i kinases involved in the control of the kinase activation, substrate presentation and downstream signaling pathways... - Solution NMR structure of proteorhodopsinSina Reckel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt Germany
Angew Chem Int Ed Engl 50:11942-6. 2011..Restraints from residual dipolar couplings improved the structural accuracy... - Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthaseAlena Busche
Institute of Biophysical Chemistry, Goethe University Frankfurt and Center for Biomolecular Magnetic Resonance, Frankfurt am Main, Germany
ACS Chem Biol 7:378-86. 2012..Since the CurA Hal accepts substrate only when presented by one of the ACPs within the ACP(I)-ACP(II)-ACP(III) tridomain, our data provide insight into the specificity of the chlorination reaction... - The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditionsRobert Hänsel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max von Laue Str 9, 60438 Frankfurt Main, Germany
Nucleic Acids Res 39:5768-75. 2011..laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic... - Tracing the protectors path from the germ line to the genomeDaniel Coutandin
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Macromolecular Complexes, Goethe University, 60438 Frankfurt Main, Germany
Proc Natl Acad Sci U S A 107:15318-25. 2010..This suggests that p53 is the most recent evolutionary member of this protein family and predicts a mechanism for p53 activation... - Structural investigation of the C-terminal catalytic fragment of presenilin 1Solmaz Sobhanifar
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Macromolecular Complexes, Goethe University, 60438 Frankfurt Main, Germany
Proc Natl Acad Sci U S A 107:9644-9. 2010..These include a putative half-membrane-spanning helix N-terminally harboring the catalytic aspartate, a severely kinked helical structure toward the C terminus as well as a soluble helix in the assumed-to-be unstructured N-terminal loop...