Nils Johnsson

Summary

Affiliation: Forschungszentrum Karlsruhe
Country: Germany

Publications

  1. ncbi request reprint A split-ubiquitin-based assay detects the influence of mutations on the conformational stability of the p53 DNA binding domain in vivo
    Nils Johnsson
    Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe, Karlsruhe, Germany
    FEBS Lett 531:259-64. 2002
  2. ncbi request reprint Protein chemistry on the surface of living cells
    Nils Johnsson
    Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021 Karlsruhe, Germany
    Chembiochem 6:47-52. 2005
  3. ncbi request reprint A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics
    Nils Johnsson
    Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021 Karlsruhe, Germany
    Chembiochem 4:803-10. 2003
  4. ncbi request reprint The split-ubiquitin sensor: measuring interactions and conformational alterations of proteins in vivo
    Christoph Reichel
    Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Eggenstein Leopoldshafen, Germany
    Methods Enzymol 399:757-76. 2005
  5. ncbi request reprint Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution
    Petra Tafelmeyer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 11:681-9. 2004
  6. pmc Recognition of a subset of signal sequences by Ssh1p, a Sec61p-related protein in the membrane of endoplasmic reticulum of yeast Saccharomyces cerevisiae
    Sandra Wittke
    Max Delbrück Laboratorium, 50829 Cologne, Germany
    Mol Biol Cell 13:2223-32. 2002
  7. ncbi request reprint Protein kinase CK2 phosphorylates Sec63p to stimulate the assembly of the endoplasmic reticulum protein translocation apparatus
    Xian Wang
    Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe, Postfach 3640, 76021 Karlsruhe, Germany
    J Cell Sci 118:723-32. 2005
  8. ncbi request reprint Multicolor imaging of cell surface proteins
    Laura Vivero-Pol
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 127:12770-1. 2005
  9. ncbi request reprint Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome
    Jörg H Eckert
    Ruhr Universitat Bochum, Institut fur Physiologische Chemie, Medizinische Fakultat, 44780 Bochum, Germany
    J Cell Sci 116:3623-34. 2003
  10. ncbi request reprint Specific labeling of cell surface proteins with chemically diverse compounds
    Nathalie George
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 126:8896-7. 2004

Collaborators

  • Kai Johnsson
  • Nathalie George
  • Christoph Reichel
  • Xian Wang
  • Laura Vivero-Pol
  • Petra Tafelmeyer
  • Jörg H Eckert
  • Maik Kindermann
  • Sandra Wittke
  • Holger Krumm
  • Horst Vogel
  • Horst Pick
  • Markus Albertsen
  • Martin Dünnwald

Detail Information

Publications11

  1. ncbi request reprint A split-ubiquitin-based assay detects the influence of mutations on the conformational stability of the p53 DNA binding domain in vivo
    Nils Johnsson
    Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe, Karlsruhe, Germany
    FEBS Lett 531:259-64. 2002
    ..A variation of this assay allows visualizing the difference in stability between the wild-type p53 core and the mutant p53(V143A) by a simple growth assay...
  2. ncbi request reprint Protein chemistry on the surface of living cells
    Nils Johnsson
    Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021 Karlsruhe, Germany
    Chembiochem 6:47-52. 2005
    ..Here we discuss a new approach for the specific labeling of fusion proteins of carrier proteins with synthetic compounds on cell surfaces and describe how this approach can be used to investigate the properties of the labeled molecules...
  3. ncbi request reprint A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics
    Nils Johnsson
    Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021 Karlsruhe, Germany
    Chembiochem 4:803-10. 2003
  4. ncbi request reprint The split-ubiquitin sensor: measuring interactions and conformational alterations of proteins in vivo
    Christoph Reichel
    Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Eggenstein Leopoldshafen, Germany
    Methods Enzymol 399:757-76. 2005
    ....
  5. ncbi request reprint Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution
    Petra Tafelmeyer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 11:681-9. 2004
    ..This powerful selection complements the repertoire of the currently used split-protein sensors and provides a new tool for high-throughput interaction screening...
  6. pmc Recognition of a subset of signal sequences by Ssh1p, a Sec61p-related protein in the membrane of endoplasmic reticulum of yeast Saccharomyces cerevisiae
    Sandra Wittke
    Max Delbrück Laboratorium, 50829 Cologne, Germany
    Mol Biol Cell 13:2223-32. 2002
    ....
  7. ncbi request reprint Protein kinase CK2 phosphorylates Sec63p to stimulate the assembly of the endoplasmic reticulum protein translocation apparatus
    Xian Wang
    Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe, Postfach 3640, 76021 Karlsruhe, Germany
    J Cell Sci 118:723-32. 2005
    ..These findings show that phosphorylation of Sec63p is required for tightly recruiting the putative signal-sequence-binding subunit Sec62p to the Sec complex...
  8. ncbi request reprint Multicolor imaging of cell surface proteins
    Laura Vivero-Pol
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 127:12770-1. 2005
    ..In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells...
  9. ncbi request reprint Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome
    Jörg H Eckert
    Ruhr Universitat Bochum, Institut fur Physiologische Chemie, Medizinische Fakultat, 44780 Bochum, Germany
    J Cell Sci 116:3623-34. 2003
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions...
  10. ncbi request reprint Specific labeling of cell surface proteins with chemically diverse compounds
    Nathalie George
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 126:8896-7. 2004
    ..The specificity and versatility of the labeling should allow it to become an important tool for studying and manipulating cell surface proteins and for complementing existing approaches in cell surface engineering...
  11. ncbi request reprint Covalent and selective immobilization of fusion proteins
    Maik Kindermann
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 125:7810-1. 2003
    ....