Research Topics
Genomes and Genes | Wolfgang FischleSummaryCountry: Germany Publications
| Collaborators
|
Detail Information
Publications
Epigenetic markers and their cross-talkStefan Winter
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Essays Biochem 48:45-61. 2010..In the present chapter, we discuss fundamental biochemical principles of modification cross-talk and reflect on the interplay of chromatin marks in cellular signalling, cell-cycle progression and cell-fate determination...
Histone and chromatin cross-talkWolfgang Fischle
Department of Biochemistry and Molecular Genetics, University of Virginia, Health Sciences Center, 1300 Jefferson Park Avenue, Charlottesville, VA 22908-0733, USA
Curr Opin Cell Biol 15:172-83. 2003..Different layers of cross-talk between several components of this complex regulatory system are emerging, and these epigenetic circuits are the focus of this review...- One, two, three: how histone methylation is readWolfgang Fischle
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
Epigenomics 4:641-53. 2012..These contain specialized modules that are recruited to chromatin in a methylation site- and state-specific manner. Besides the molecular mechanisms of interaction, patterns of regulation of the binding proteins are becoming evident... - Talk is cheap--cross-talk in establishment, maintenance, and readout of chromatin modificationsWolfgang Fischle
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
Genes Dev 22:3375-82. 2008..In this issue of Genes & Development, Adhvaryu and Selker (3391-3396) provide novel insights into an intricate regulatory network involving histone phosphorylation, histone methylation, and DNA methylation... 
Specificity of the chromodomain Y chromosome family of chromodomains for lysine-methylated ARK(S/T) motifsWolfgang Fischle
Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
J Biol Chem 283:19626-35. 2008..Our studies underscore the significance of subtle sequence differences in a conserved signaling module for diverse epigenetic regulatory pathways...- Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomainsWolfgang Fischle
Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
Genes Dev 17:1870-81. 2003..The Pc chromodomain distinguishes its methylation target on the H3 tail via an extended recognition groove that binds five additional residues preceding the ARKS motif... - Methylation of lysine 9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1β with the nucleosomeFrancesca Munari
Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
J Biol Chem 287:33756-65. 2012..Our results report the first detailed structural analysis of a dynamic protein-nucleosome complex directed by a histone modification and provide a conceptual framework for understanding similar interactions in the context of chromatin... - Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patternsMiroslav Nikolov
Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
Mol Cell Proteomics 10:M110.005371. 2011..Our proof-of-principle studies show that chromatin templates with defined modification patterns can be used to decipher how the histone code is read and translated... - HP1 recruits activity-dependent neuroprotective protein to H3K9me3 marked pericentromeric heterochromatin for silencing of major satellite repeatsKerstin Mosch
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
PLoS ONE 6:e15894. 2011..Our results identify a novel factor in the translation of H3K9me3 at pericentromeric heterochromatin that regulates transcription... - Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptidesSteven A Jacobs
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
Methods Enzymol 376:131-48. 2004 - Molecular architecture of the human Prp19/CDC5L complexMichael Grote
Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
Mol Cell Biol 30:2105-19. 2010..Our findings not only elucidate the molecular organization of the hPrp19/CDC5L complex but also provide insights into potential protein-protein interactions at the core of the catalytically active spliceosome... - Role of histone modifications in defining chromatin structure and functionKathy A Gelato
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
Biol Chem 389:353-63. 2008..We review the properties of various chromatin elements and the apparent links of histone modifications with chromatin organization and functional output... - H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva developmentNora Koester-Eiserfunke
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
PLoS Genet 7:e1002017. 2011..Our results also introduce a mechanistic link between LIN-61 function and biology, and they establish interplay of the H3K9me2/3 binding proteins, LIN-61 and HPL-2, as well as the H3K9MT MET-2 in distinct developmental pathways... - Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin associationHenriette Franz
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
J Biol Chem 284:35049-59. 2009..We suggest that similar multivalent binding stably anchors other histone modification binding factors on their target chromatin regions... - Systematic analysis of histone modification readoutMiroslav Nikolov
Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
Mol Biosyst 9:182-94. 2013..We summarize the experimental approaches that are used to determine histone modification readout and discuss complexities that are emerging within this regulatory system... - HIS-24 linker histone and SIR-2.1 deacetylase induce H3K27me3 in the Caenorhabditis elegans germ lineMartina Wirth
Max Planck Institute for Biophysical Chemistry, Laboratory of Chromatin Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
Mol Cell Biol 29:3700-9. 2009..Overall, our data indicate that SIR-2.1 and HIS-24 contribute to the propagation of a specialized chromatin state at the subtelomeric regions and elsewhere in the genome... - Control of cytomegalovirus lytic gene expression by histone acetylationJane C Murphy
Department of Medicine, University of Cambridge, Cambridge CB2 2QQ, UK
EMBO J 21:1112-20. 2002.... - Modulation of 14-3-3 interaction with phosphorylated histone H3 by combinatorial modification patternsStefan Winter
Max F Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Vienna, Austria
Cell Cycle 7:1336-42. 2008..Here we discuss the binding of 14-3-3 proteins to histone H3 in detail and putative biological implications of these interactions... - Dynamic regulation of effector protein binding to histone modifications: the biology of HP1 switchingHolger L Dormann
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York, USA
Cell Cycle 5:2842-51. 2006.... - Beyond the double helix: writing and reading the histone codeYanming Wang
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
Novartis Found Symp 259:3-17; discussion 17-21, 163-9. 2004..As this modification is not found during mitotic chromosome condensation, these findings suggest the intriguing possibility that a unique 'death' mark exists for chromatin condensation during apoptosis... - In nucleo enzymatic assays for the identification and characterization of histone modifying activitiesWolfgang Fischle
Laboratory of Chromatin Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Methods 36:362-7. 2005..Simple methods for the analysis of histone modifications using these assays are discussed... - Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylationWolfgang Fischle
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
Nature 438:1116-22. 2005..These findings establish a regulatory mechanism of protein-protein interactions, through a combinatorial readout of two adjacent post-translational modifications: a stable methylation and a dynamic phosphorylation mark... - A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activitiesTetyana Klymenko
Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Genes Dev 20:1110-22. 2006..We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state... - Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatinKe Zhang
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Struct Mol Biol 15:381-8. 2008..Our analyses delineate sequential steps for the assembly of heterochromatic domains and suggest that the ability of Clr4 to both 'write' and 'read' H3K9me facilitates heterochromatin maintenance through successive cell divisions... - 14-3-3 proteins recognize a histone code at histone H3 and are required for transcriptional activationStefan Winter
Max F Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Vienna, Austria
EMBO J 27:88-99. 2008..Finally, siRNA-mediated loss of 14-3-3 proteins abolishes the transcriptional activation of HDAC1. Together our data indicate that 14-3-3 proteins are crucial mediators of histone phosphoacetylation signals... - Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD fingerHaitao Li
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Mol Cell 28:677-91. 2007.... - Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midlegClemens Grimm
European Molecular Biology Laboratory, Grenoble Outstation, 6 rue Jules Horowitz, 38042 Grenoble, France
EMBO Rep 8:1031-7. 2007..Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes... - The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeastTakatomi Yamada
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell 20:173-85. 2005.... - Binary switches and modification cassettes in histone biology and beyondWolfgang Fischle
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
Nature 425:475-9. 2003..Our ideas might also apply to non-histone proteins and are open to direct experimental examination... - HDAC7, a thymus-specific class II histone deacetylase, regulates Nur77 transcription and TCR-mediated apoptosisFranck Dequiedt
Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco 94141, USA
Immunity 18:687-98. 2003..Inhibition of HDAC7 expression by RNA interference causes increased apoptosis in response to TCR activation. These observations define HDAC7 as a regulator of Nur77 and apoptosis in developing thymocytes... - Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoRWolfgang Fischle
Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, CA 94141, USA
Mol Cell 9:45-57. 2002..These observations indicate that class II HDACs regulate transcription by bridging the enzymatically active SMRT/N-CoR-HDAC3 complex and select transcription factors independently of any intrinsic HDAC activity...