Wolfgang Fischle

Summary

Country: Germany

Publications

  1. doi request reprint Epigenetic markers and their cross-talk
    Stefan Winter
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Essays Biochem 48:45-61. 2010
  2. ncbi request reprint Histone and chromatin cross-talk
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia, Health Sciences Center, 1300 Jefferson Park Avenue, Charlottesville, VA 22908 0733, USA
    Curr Opin Cell Biol 15:172-83. 2003
  3. pmc HP1 recruits activity-dependent neuroprotective protein to H3K9me3 marked pericentromeric heterochromatin for silencing of major satellite repeats
    Kerstin Mosch
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS ONE 6:e15894. 2011
  4. pmc H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva development
    Nora Koester-Eiserfunke
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS Genet 7:e1002017. 2011
  5. pmc Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin association
    Henriette Franz
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Biol Chem 284:35049-59. 2009
  6. pmc Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns
    Miroslav Nikolov
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Cell Proteomics 10:M110.005371. 2011
  7. doi request reprint One, two, three: how histone methylation is read
    Wolfgang Fischle
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Epigenomics 4:641-53. 2012
  8. pmc Specificity of the chromodomain Y chromosome family of chromodomains for lysine-methylated ARK(S/T) motifs
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    J Biol Chem 283:19626-35. 2008
  9. doi request reprint Talk is cheap--cross-talk in establishment, maintenance, and readout of chromatin modifications
    Wolfgang Fischle
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Genes Dev 22:3375-82. 2008
  10. pmc Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    Genes Dev 17:1870-81. 2003

Collaborators

Detail Information

Publications37

  1. doi request reprint Epigenetic markers and their cross-talk
    Stefan Winter
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Essays Biochem 48:45-61. 2010
    ..In the present chapter, we discuss fundamental biochemical principles of modification cross-talk and reflect on the interplay of chromatin marks in cellular signalling, cell-cycle progression and cell-fate determination...
  2. ncbi request reprint Histone and chromatin cross-talk
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia, Health Sciences Center, 1300 Jefferson Park Avenue, Charlottesville, VA 22908 0733, USA
    Curr Opin Cell Biol 15:172-83. 2003
    ..Different layers of cross-talk between several components of this complex regulatory system are emerging, and these epigenetic circuits are the focus of this review...
  3. pmc HP1 recruits activity-dependent neuroprotective protein to H3K9me3 marked pericentromeric heterochromatin for silencing of major satellite repeats
    Kerstin Mosch
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS ONE 6:e15894. 2011
    ..Our results identify a novel factor in the translation of H3K9me3 at pericentromeric heterochromatin that regulates transcription...
  4. pmc H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva development
    Nora Koester-Eiserfunke
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS Genet 7:e1002017. 2011
    ..Our results also introduce a mechanistic link between LIN-61 function and biology, and they establish interplay of the H3K9me2/3 binding proteins, LIN-61 and HPL-2, as well as the H3K9MT MET-2 in distinct developmental pathways...
  5. pmc Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin association
    Henriette Franz
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Biol Chem 284:35049-59. 2009
    ..We suggest that similar multivalent binding stably anchors other histone modification binding factors on their target chromatin regions...
  6. pmc Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns
    Miroslav Nikolov
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Cell Proteomics 10:M110.005371. 2011
    ..Our proof-of-principle studies show that chromatin templates with defined modification patterns can be used to decipher how the histone code is read and translated...
  7. doi request reprint One, two, three: how histone methylation is read
    Wolfgang Fischle
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Epigenomics 4:641-53. 2012
    ..These contain specialized modules that are recruited to chromatin in a methylation site- and state-specific manner. Besides the molecular mechanisms of interaction, patterns of regulation of the binding proteins are becoming evident...
  8. pmc Specificity of the chromodomain Y chromosome family of chromodomains for lysine-methylated ARK(S/T) motifs
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    J Biol Chem 283:19626-35. 2008
    ..Our studies underscore the significance of subtle sequence differences in a conserved signaling module for diverse epigenetic regulatory pathways...
  9. doi request reprint Talk is cheap--cross-talk in establishment, maintenance, and readout of chromatin modifications
    Wolfgang Fischle
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Genes Dev 22:3375-82. 2008
    ..In this issue of Genes & Development, Adhvaryu and Selker (3391-3396) provide novel insights into an intricate regulatory network involving histone phosphorylation, histone methylation, and DNA methylation...
  10. pmc Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains
    Wolfgang Fischle
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    Genes Dev 17:1870-81. 2003
    ..The Pc chromodomain distinguishes its methylation target on the H3 tail via an extended recognition groove that binds five additional residues preceding the ARKS motif...
  11. pmc Methylation of lysine 9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1β with the nucleosome
    Francesca Munari
    Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Biol Chem 287:33756-65. 2012
    ..Our results report the first detailed structural analysis of a dynamic protein-nucleosome complex directed by a histone modification and provide a conceptual framework for understanding similar interactions in the context of chromatin...
  12. pmc Structural plasticity in human heterochromatin protein 1β
    Francesca Munari
    Department for NMR based Structural Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS ONE 8:e60887. 2013
    ..The structural plasticity of hHP1β supports its ability to bind and connect a wide variety of binding partners in epigenetic processes...
  13. ncbi request reprint Characterization of the effects of phosphorylation by CK2 on the structure and binding properties of human HP1β
    Francesca Munari
    Department for NMR based Structural Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany German Center for Neurodegenerative Diseases DZNE, Gottingen, Germany
    FEBS Lett 588:1094-9. 2014
    ..Phosphorylation at these sites results in localized conformational changes in HP1β that do not compromise the ability of the protein to bind chromatin. ..
  14. pmc Molecular architecture of the human Prp19/CDC5L complex
    Michael Grote
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Mol Cell Biol 30:2105-19. 2010
    ..Our findings not only elucidate the molecular organization of the hPrp19/CDC5L complex but also provide insights into potential protein-protein interactions at the core of the catalytically active spliceosome...
  15. ncbi request reprint Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides
    Steven A Jacobs
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
    Methods Enzymol 376:131-48. 2004
  16. doi request reprint A cascade of histone modifications induces chromatin condensation in mitosis
    Bryan J Wilkins
    Free Floater Junior Research Group Applied Synthetic Biology, Institute for Microbiology and Genetics, Georg August University Gottingen, 37077 Gottingen, Germany
    Science 343:77-80. 2014
    ..This cascade of events provides a condensin-independent driving force of chromatin hypercondensation during mitosis. ..
  17. doi request reprint Role of histone modifications in defining chromatin structure and function
    Kathy A Gelato
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Biol Chem 389:353-63. 2008
    ..We review the properties of various chromatin elements and the apparent links of histone modifications with chromatin organization and functional output...
  18. pmc Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases
    Martina Wirth
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mitochondrion 13:705-20. 2013
    ..Nevertheless, no changes in mPC acetylation levels and enzymatic activity could be detected upon overexpression or loss of functional SIRT4. ..
  19. ncbi request reprint Accessibility of Different Histone H3-Binding Domains of UHRF1 Is Allosterically Regulated by Phosphatidylinositol 5-Phosphate
    Kathy A Gelato
    Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Mol Cell 54:905-19. 2014
    ..Our results define an allosteric mechanism controlling heterochromatin association of an essential regulatory protein of epigenetic states and identify a functional role for enigmatic nuclear phosphatidylinositol phosphates. ..
  20. pmc HIS-24 linker histone and SIR-2.1 deacetylase induce H3K27me3 in the Caenorhabditis elegans germ line
    Martina Wirth
    Max Planck Institute for Biophysical Chemistry, Laboratory of Chromatin Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Mol Cell Biol 29:3700-9. 2009
    ..Overall, our data indicate that SIR-2.1 and HIS-24 contribute to the propagation of a specialized chromatin state at the subtelomeric regions and elsewhere in the genome...
  21. doi request reprint Systematic analysis of histone modification readout
    Miroslav Nikolov
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biosyst 9:182-94. 2013
    ..We summarize the experimental approaches that are used to determine histone modification readout and discuss complexities that are emerging within this regulatory system...
  22. pmc Quantitative assessment of protein interaction with methyl-lysine analogues by hybrid computational and experimental approaches
    Daniel Seeliger
    Theoretische und Computergestützte Biophysik and Chromatin Biochemie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    ACS Chem Biol 7:150-4. 2012
    ..We suggest that quantitative binding parameters for defined ligands in general can be derived by this method with remarkable accuracy...
  23. pmc Modulation of 14-3-3 interaction with phosphorylated histone H3 by combinatorial modification patterns
    Stefan Winter
    Max F Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Vienna, Austria
    Cell Cycle 7:1336-42. 2008
    ..Here we discuss the binding of 14-3-3 proteins to histone H3 in detail and putative biological implications of these interactions...
  24. pmc Control of cytomegalovirus lytic gene expression by histone acetylation
    Jane C Murphy
    Department of Medicine, University of Cambridge, Cambridge CB2 2QQ, UK
    EMBO J 21:1112-20. 2002
    ....
  25. ncbi request reprint Beyond the double helix: writing and reading the histone code
    Yanming Wang
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Novartis Found Symp 259:3-17; discussion 17-21, 163-9. 2004
    ..As this modification is not found during mitotic chromosome condensation, these findings suggest the intriguing possibility that a unique 'death' mark exists for chromatin condensation during apoptosis...
  26. ncbi request reprint Dynamic regulation of effector protein binding to histone modifications: the biology of HP1 switching
    Holger L Dormann
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York, USA
    Cell Cycle 5:2842-51. 2006
    ....
  27. pmc A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities
    Tetyana Klymenko
    Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Genes Dev 20:1110-22. 2006
    ..We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state...
  28. ncbi request reprint In nucleo enzymatic assays for the identification and characterization of histone modifying activities
    Wolfgang Fischle
    Laboratory of Chromatin Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Methods 36:362-7. 2005
    ..Simple methods for the analysis of histone modifications using these assays are discussed...
  29. ncbi request reprint Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation
    Wolfgang Fischle
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Nature 438:1116-22. 2005
    ..These findings establish a regulatory mechanism of protein-protein interactions, through a combinatorial readout of two adjacent post-translational modifications: a stable methylation and a dynamic phosphorylation mark...
  30. ncbi request reprint HDAC7, a thymus-specific class II histone deacetylase, regulates Nur77 transcription and TCR-mediated apoptosis
    Franck Dequiedt
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco 94141, USA
    Immunity 18:687-98. 2003
    ..Inhibition of HDAC7 expression by RNA interference causes increased apoptosis in response to TCR activation. These observations define HDAC7 as a regulator of Nur77 and apoptosis in developing thymocytes...
  31. ncbi request reprint Binary switches and modification cassettes in histone biology and beyond
    Wolfgang Fischle
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Nature 425:475-9. 2003
    ..Our ideas might also apply to non-histone proteins and are open to direct experimental examination...
  32. ncbi request reprint The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast
    Takatomi Yamada
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 20:173-85. 2005
    ....
  33. pmc Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg
    Clemens Grimm
    European Molecular Biology Laboratory, Grenoble Outstation, 6 rue Jules Horowitz, 38042 Grenoble, France
    EMBO Rep 8:1031-7. 2007
    ..Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes...
  34. ncbi request reprint Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 28:677-91. 2007
    ....
  35. pmc 14-3-3 proteins recognize a histone code at histone H3 and are required for transcriptional activation
    Stefan Winter
    Max F Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Vienna, Austria
    EMBO J 27:88-99. 2008
    ..Finally, siRNA-mediated loss of 14-3-3 proteins abolishes the transcriptional activation of HDAC1. Together our data indicate that 14-3-3 proteins are crucial mediators of histone phosphoacetylation signals...
  36. doi request reprint Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin
    Ke Zhang
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 15:381-8. 2008
    ..Our analyses delineate sequential steps for the assembly of heterochromatic domains and suggest that the ability of Clr4 to both 'write' and 'read' H3K9me facilitates heterochromatin maintenance through successive cell divisions...
  37. ncbi request reprint Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR
    Wolfgang Fischle
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, CA 94141, USA
    Mol Cell 9:45-57. 2002
    ..These observations indicate that class II HDACs regulate transcription by bridging the enzymatically active SMRT/N-CoR-HDAC3 complex and select transcription factors independently of any intrinsic HDAC activity...