U Fischer

Summary

Country: Germany

Publications

  1. pmc Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycle
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    Nucleic Acids Res 27:4128-34. 1999
  2. ncbi request reprint The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
    Cell 82:475-83. 1995
  3. ncbi request reprint Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse
    H Hornig
    Max Planck Institut fur Molekulare Genetik, Berlin
    Eur J Biochem 182:45-50. 1989
  4. ncbi request reprint Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy
    D Buhler
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Hum Mol Genet 8:2351-7. 1999
  5. pmc SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome
    G Meister
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, University of Gottingen, Biochemie II, Herrmann Düker Weg 12, D 37073 Gottingen, Germany
    EMBO J 20:2304-14. 2001
  6. ncbi request reprint The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis
    U Fischer
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cell 90:1023-9. 1997
  7. pmc HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import
    R A Fouchier
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 16:4531-9. 1997
  8. pmc Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev
    R A Fridell
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:2936-40. 1996
  9. ncbi request reprint Cell surface trafficking of Fas in NIT-1 cells and dissection of surface and total Fas expression
    P Augstein
    Gerhardt Katsch Institute of Diabetes Karlsburg e V, Greifswalder Strasse 11e, 17495 Karlsburg, Germany
    Biochem Biophys Res Commun 290:443-51. 2002
  10. ncbi request reprint Molecular and functional analysis of intragenic SMN1 mutations in patients with spinal muscular atrophy
    Y Sun
    Institute of Human Genetics, University of Cologne, Cologne, Germany
    Hum Mutat 25:64-71. 2005

Collaborators

  • I W Mattaj
  • W C Boelens
  • V W Pollard
  • BRYAN CULLEN
  • Fu Sheng Wang
  • Brunhilde Wirth
  • P Augstein
  • D A Jans
  • G Meister
  • D Buhler
  • K Janssen
  • H Brahms
  • S Hannus
  • Y Sun
  • R A Fouchier
  • R Luhrmann
  • S Jablonka
  • P Selenko
  • M H Malim
  • B Laggerbauer
  • F Lottspeich
  • B E Meyer
  • K Schulze-Osthoff
  • T G Hofmann
  • R T Hay
  • M Grimmler
  • F Schoenen
  • V Schwarzer
  • J H Simon
  • Q Liu
  • R A Fridell
  • L Meheus
  • S Wiese
  • R Pillai
  • M Sattler
  • C Eggert
  • C Kambach
  • M Sendtner
  • T Baars
  • S Fakan
  • E Hartmann
  • M Bandilla
  • V de Brabandere
  • O Plöttner
  • G Stier
  • R Sprangers
  • M Romano
  • M Zobawa
  • B Seraphin
  • V Raker
  • A V Albright
  • W Kammerloher
  • F Gonzalez-Scarano
  • G Dreyfuss
  • J L Meinkoth
  • A R Schäffner
  • G P Piechottka
  • H Hornig
  • M Costas
  • A Rauh

Detail Information

Publications22

  1. pmc Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycle
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    Nucleic Acids Res 27:4128-34. 1999
    ..Finally, we demonstrate that Rev/RRE-activated RNA export is, like other nuclear export pathways, linked to the Ran-GTPase cycle...
  2. ncbi request reprint The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
    Cell 82:475-83. 1995
    ..BSA-R was itself exported from the nucleus in an active, saturable manner. Thus, the Rev activation domain constitutes a nuclear export signal that redirects RRE-containing viral RNAs to a non-mRNA export pathway...
  3. ncbi request reprint Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse
    H Hornig
    Max Planck Institut fur Molekulare Genetik, Berlin
    Eur J Biochem 182:45-50. 1989
    ..A 72-bp alternative exon harboring an in-frame termination codon was also found in the mouse 70-kDa gene and shows, surprisingly, 100% nucleotide identity to its human counterpart...
  4. ncbi request reprint Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy
    D Buhler
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Hum Mol Genet 8:2351-7. 1999
    ..Thus, our data show that SMN is an essential U snRNP assembly factor and establish a direct correlation between defects in the biogenesis of U snRNPs and SMA...
  5. pmc SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome
    G Meister
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, University of Gottingen, Biochemie II, Herrmann Düker Weg 12, D 37073 Gottingen, Germany
    EMBO J 20:2304-14. 2001
    ..A candidate that may mediate this interaction, namely the U4/U6-90 kDa protein, has been identified. We suggest that SMNrp, as a U2 snRNP-associated protein, facilitates the recruitment of the [U4/U6.U5] tri-snRNP to the pre-spliceosome...
  6. ncbi request reprint The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis
    U Fischer
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cell 90:1023-9. 1997
    ..Thus, the SMN-SIP1 complex is directly involved in the biogenesis of spliceosomal snRNPs. Defects in spliceosomal snRNP biogenesis may, therefore, be the cause of SMA...
  7. pmc HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import
    R A Fouchier
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 16:4531-9. 1997
    ..Taken together, these observations suggest that proteins other than MA supply the NLS(s) that enable HIV-1 to infect non-dividing cells...
  8. pmc Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev
    R A Fridell
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:2936-40. 1996
    ..Thus, this shared protein motif may play an analogous role in mediating the nuclear export of both late HIV-1 RNAs and 5S rRNA transcripts...
  9. ncbi request reprint Cell surface trafficking of Fas in NIT-1 cells and dissection of surface and total Fas expression
    P Augstein
    Gerhardt Katsch Institute of Diabetes Karlsburg e V, Greifswalder Strasse 11e, 17495 Karlsburg, Germany
    Biochem Biophys Res Commun 290:443-51. 2002
    ..It is concluded that inflammatory insults specifically induce translocation of Fas to the beta-cell surface and that interference with cell surface Fas expression is a new strategy to improve beta-cell survival in inflamed islets...
  10. ncbi request reprint Molecular and functional analysis of intragenic SMN1 mutations in patients with spinal muscular atrophy
    Y Sun
    Institute of Human Genetics, University of Cologne, Cologne, Germany
    Hum Mutat 25:64-71. 2005
    ..However, all mutations, including those in exon 2a, a region shown to be important for the binding of SMN to SIP1, do not disturb the interaction of SMN to SIP1...
  11. pmc Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein
    H Brahms
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Gottingen, Germany
    RNA 7:1531-42. 2001
    ..Our demonstration of sDMAs in LSm4 suggests additional functions of sDMAs in tri-UsnRNP biogenesis and mRNA decay. Our findings also have interesting implications for the understanding of the aetiology of spinal muscular atrophy (SMA)...
  12. ncbi request reprint A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs
    G Meister
    Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    Nat Cell Biol 3:945-9. 2001
    ..These data provide the first direct evidence that a complex containing SMN and Gemin2 mediates the active assembly of spliceosomal U snRNPs...
  13. ncbi request reprint KUB3 amplification and overexpression in human gliomas
    U Fischer
    Institut fur Humangenetik, Universitat des Saarlandes, Homburg Saar, Germany
    Glia 36:1-10. 2001
    ..The KUB3 amplification and overexpression may interfere with the function of KUB3 in the DNA-PK complex involved in the maintenance of genome stability and reduction of mutation frequency...
  14. ncbi request reprint Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln
    G Meister
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Curr Biol 11:1990-4. 2001
    ..Our data suggest that the pICln complex regulates an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex...
  15. ncbi request reprint Co-regulation of survival of motor neuron (SMN) protein and its interactor SIP1 during development and in spinal muscular atrophy
    S Jablonka
    Klinische Forschergruppe Neuroregeneration, Department of Neurology, University of Wurzburg, Josef Schneider Strasse 11, D 97080 Wurzburg, Germany
    Hum Mol Genet 10:497-505. 2001
    ..The finding that expression of Sip1 and Smn is tightly co-regulated, together with the unique localization of Smn in neurites, may help in understanding the motor neuron-specific defects observed in SMA patients...
  16. ncbi request reprint SMN tudor domain structure and its interaction with the Sm proteins
    P Selenko
    Structural and Computational Biology, EMBL, Meyerhofstr 1, D 69012 Heidelberg, Germany
    Nat Struct Biol 8:27-31. 2001
    ..Our data provide a structural basis for a molecular defect underlying SMA...
  17. ncbi request reprint Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins
    G Meister
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Hum Mol Genet 9:1977-86. 2000
    ..This shows that the SMN-Sm protein interaction is not restricted to the cytoplasm. Our data imply that nuclear SMN affects splicing by modulating the Sm protein composition of U snRNPs...
  18. ncbi request reprint The Schizosaccharomyces pombe protein Yab8p and a novel factor, Yip1p, share structural and functional similarity with the spinal muscular atrophy-associated proteins SMN and SIP1
    S Hannus
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Hum Mol Genet 9:663-74. 2000
    ..Our studies provide a model system to study the cellular function of SMN in yeast, and should help in understanding the molecular events leading to SMA...
  19. pmc Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex
    R A Fouchier
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    J Virol 72:6004-13. 1998
    ..These findings not only demonstrate that Vpr harbors a bona fide NLS but also raise the possibility that one (or more) of Vpr's functions may take place at the NPC...
  20. ncbi request reprint The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins
    Q Liu
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cell 90:1013-21. 1997
    ..These findings suggest a role for SMN and SIP1 in spliceosomal snRNP biogenesis and function and provide a likely molecular mechanism for the cause of SMA...
  21. ncbi request reprint Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system
    W Kammerloher
    Institut fur Biochemie, Ludwig Maximilians Universitat, Munchen, Germany
    Plant J 6:187-99. 1994
    ..Nevertheless, Northern and Western analyses showed that the PIP genes are constitutively and possibly even redundantly expressed from the small A. thaliana genome...
  22. ncbi request reprint Apoptin is modified by SUMO conjugation and targeted to promyelocytic leukemia protein nuclear bodies
    K Janssen
    Institute of Molecular Medicine, Heinrich Heine University, Dusseldorf, Germany
    Oncogene 26:1557-66. 2007
    ..Our results therefore suggest that apoptin kills tumor cells independently of PML and sumoylation, however, the interaction of apoptin with PML and small ubiquitin-like modifier (SUMO) proteins might be relevant for CAV replication...