K Smalla

Summary

Affiliation: Federal Biological Research Centre for Agriculture and Forestry
Country: Germany

Publications

  1. pmc Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids
    K Smalla
    Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, D 38104 Braunschweig, Germany
    Appl Environ Microbiol 66:4854-62. 2000
  2. ncbi request reprint Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: do the different methods provide similar results?
    Kornelia Smalla
    Biologische Bundesanstalt für Land und Forstwirtschaft BBA, Messeweg 11 12, 38104 Braunschweig, Germany
    J Microbiol Methods 69:470-9. 2007
  3. ncbi request reprint Horizontal gene transfer between bacteria
    Holger Heuer
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    Environ Biosafety Res 6:3-13. 2007
  4. ncbi request reprint Screening of rhizosphere and soil bacteria for transformability
    Babette Richter
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, 38104, Braunschweig, Germany
    Environ Biosafety Res 6:91-9. 2007
  5. pmc Increased abundance of IncP-1beta plasmids and mercury resistance genes in mercury-polluted river sediments: first discovery of IncP-1beta plasmids with a complex mer transposon as the sole accessory element
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry BBA, Messeweg 11 12, 38104 Braunschweig, Germany
    Appl Environ Microbiol 72:7253-9. 2006
  6. ncbi request reprint Field releases of genetically modified micro-organisms
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, 38104 Braunschweig, Germany
    Environ Biosafety Res 2:65-8. 2003
  7. pmc Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed
    K Smalla
    Federal Biological Research Centre for Agriculture and Forestry, D 38104 Braunschweig, D 18051 Rostock, Germany
    Appl Environ Microbiol 67:4742-51. 2001
  8. ncbi request reprint The prevalence and diversity of mobile genetic elements in bacterial communities of different environmental habitats: insights gained from different methodological approaches
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, D 38104 Braunschweig, Germany
    FEMS Microbiol Ecol 42:165-75. 2002
  9. ncbi request reprint Short-term effects of amoxicillin on bacterial communities in manured soil
    Chu Thi Thanh Binh
    Federal Biological Research Centre for Agriculture and Forestry, Braunschweig, Germany
    FEMS Microbiol Ecol 62:290-302. 2007
  10. pmc Diversity of ndo genes in mangrove sediments exposed to different sources of polycyclic aromatic hydrocarbon pollution
    Newton C Marcial Gomes
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    Appl Environ Microbiol 73:7392-9. 2007

Collaborators

Detail Information

Publications41

  1. pmc Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids
    K Smalla
    Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, D 38104 Braunschweig, Germany
    Appl Environ Microbiol 66:4854-62. 2000
    ..These findings suggest that IncQ plasmids play an important role in disseminating antibiotic resistance genes...
  2. ncbi request reprint Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: do the different methods provide similar results?
    Kornelia Smalla
    Biologische Bundesanstalt für Land und Forstwirtschaft BBA, Messeweg 11 12, 38104 Braunschweig, Germany
    J Microbiol Methods 69:470-9. 2007
    ....
  3. ncbi request reprint Horizontal gene transfer between bacteria
    Holger Heuer
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    Environ Biosafety Res 6:3-13. 2007
    ..In view of the use of GM crops and microbes in agricultural settings, in this mini-review we focus particularly on the presence and role of MGE in soil and plant-associated bacteria and the factors affecting gene transfer...
  4. ncbi request reprint Screening of rhizosphere and soil bacteria for transformability
    Babette Richter
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, 38104, Braunschweig, Germany
    Environ Biosafety Res 6:91-9. 2007
    ..The various transformation assays performed indicate that the proportion of rhizosphere or bulk soil bacteria which are naturally transformable is negligibly low...
  5. pmc Increased abundance of IncP-1beta plasmids and mercury resistance genes in mercury-polluted river sediments: first discovery of IncP-1beta plasmids with a complex mer transposon as the sole accessory element
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry BBA, Messeweg 11 12, 38104 Braunschweig, Germany
    Appl Environ Microbiol 72:7253-9. 2006
    ..This transposon carries a rather complex set of mer genes and is inserted between Tra1 and Tra2...
  6. ncbi request reprint Field releases of genetically modified micro-organisms
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, 38104 Braunschweig, Germany
    Environ Biosafety Res 2:65-8. 2003
  7. pmc Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed
    K Smalla
    Federal Biological Research Centre for Agriculture and Forestry, D 38104 Braunschweig, D 18051 Rostock, Germany
    Appl Environ Microbiol 67:4742-51. 2001
    ..Sequencing of dominant bands excised from the rhizosphere patterns revealed that 6 out of 10 bands resembled gram-positive bacteria. Nocardia populations were identified as strawberry-specific bands...
  8. ncbi request reprint The prevalence and diversity of mobile genetic elements in bacterial communities of different environmental habitats: insights gained from different methodological approaches
    Kornelia Smalla
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, D 38104 Braunschweig, Germany
    FEMS Microbiol Ecol 42:165-75. 2002
    ....
  9. ncbi request reprint Short-term effects of amoxicillin on bacterial communities in manured soil
    Chu Thi Thanh Binh
    Federal Biological Research Centre for Agriculture and Forestry, Braunschweig, Germany
    FEMS Microbiol Ecol 62:290-302. 2007
    ..Molecular fingerprint analysis of 16S rRNA gene fragments amplified from soil DNA revealed significant effects of manure and amoxicillin on the bacterial community of both soils...
  10. pmc Diversity of ndo genes in mangrove sediments exposed to different sources of polycyclic aromatic hydrocarbon pollution
    Newton C Marcial Gomes
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    Appl Environ Microbiol 73:7392-9. 2007
    ..The long-term impact of PAH contamination, together with the specific environmental conditions at each site, may have affected the abundance and diversity of ndo genes in sediments of urban mangroves...
  11. pmc Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments
    J Schonfeld
    Institute for Plant Virology, Microbiology, and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, 38104 Braunschweig, Germany
    Appl Environ Microbiol 69:7248-56. 2003
    ..The system was applied to survey soils from different geographic origins for the presence of R. solanacearum...
  12. doi request reprint Diverse aadA gene cassettes on class 1 integrons introduced into soil via spread manure
    Chu T T Binh
    Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn Institut, Federal Research Centre for Cultivated Plants JKI, Messeweg 11 12, D 38104 Braunschweig, Germany
    Res Microbiol 160:427-33. 2009
    ..In addition, class 1 integrons without gene cassettes were detected in total community DNA of many manures...
  13. pmc Soil type-dependent responses to phenanthrene as revealed by determining the diversity and abundance of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase genes by using a novel PCR detection system
    Guo Chun Ding
    Julius Kühn Institut, Messeweg, D 38104 Braunschweig, Germany
    Appl Environ Microbiol 76:4765-71. 2010
    ..In conclusion, sequence analysis of the amplicons obtained confirmed the specificity of the novel primer system and revealed a soil type-dependent response of PAH-RHD(alpha) gene-carrying soil bacteria to phenanthrene spiking...
  14. ncbi request reprint Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months
    Holger Heuer
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    Environ Microbiol 9:657-66. 2007
    ..The synergistic effects of manure and SDZ were still detectable after 2 months. The results suggest that manure from treated pigs enhances spread of antibiotic resistances in soil bacterial communities...
  15. ncbi request reprint Effects of site and plant species on rhizosphere community structure as revealed by molecular analysis of microbial guilds
    Rodrigo Costa
    Federal Biological Research Centre for Agriculture and Forestry, Braunschweig, Germany
    FEMS Microbiol Ecol 56:236-49. 2006
    ..and Rhizobium sp. were among the dominant ribotypes in the strawberry rhizosphere, while sequences from Arthrobacter sp. corresponded to dominant bands from oilseed rape bacterial fingerprints...
  16. ncbi request reprint Pseudomonas community structure and antagonistic potential in the rhizosphere: insights gained by combining phylogenetic and functional gene-based analyses
    Rodrigo Costa
    Federal Biological Research Centre for Agriculture and Forestry BBA, Messeweg 11 12, D 38104 Braunschweig, Germany
    Environ Microbiol 9:2260-73. 2007
    ..Partial gacA gene sequences derived from isolates, clones of the strawberry rhizosphere and DGGE bands retrieved in this study represent previously undescribed Pseudomonas gacA gene clusters as revealed by phylogenetic analysis...
  17. pmc Dynamics of fungal communities in bulk and maize rhizosphere soil in the tropics
    Newton C Marcial Gomes
    Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11 12, 38104 Braunschweig, Germany
    Appl Environ Microbiol 69:3758-66. 2003
    ....
  18. doi request reprint In vitro antagonists of Rhizoctonia solani tested on lettuce: rhizosphere competence, biocontrol efficiency and rhizosphere microbial community response
    Modupe F Adesina
    Julius Kühn Institut Federal Research Centre for Cultivated Plants JKI, D 38104 Braunschweig, Germany
    FEMS Microbiol Ecol 69:62-74. 2009
    ..Although the exact mechanism of antagonistic activity and the ecology of RU47 remain to be further explored, our results suggest that RU47 is a promising agent to control bottom rot of lettuce...
  19. ncbi request reprint Cultivation-independent analysis of Pseudomonas species in soil and in the rhizosphere of field-grown Verticillium dahliae host plants
    Rodrigo Costa
    Federal Biological Research Centre for Agriculture and Forestry BBA, Messeweg 11 12, D 38104 Braunschweig, Germany
    Environ Microbiol 8:2136-49. 2006
    ..This report provides a better understanding of how different factors drive Pseudomonas community structure and diversity in bulk and rhizosphere soils...
  20. pmc Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors
    Holger Heuer
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Microbiology, Plant Virology and Biosafety, Messeweg 11 12, D 38104 Braunschweig, Germany
    Appl Environ Microbiol 68:1325-35. 2002
    ....
  21. pmc Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium dahliae kleb
    Gabriele Berg
    University of Rostock, Fachbereich Biowissenschaften, Microbiology, D 18051 Rostock, Germany
    Appl Environ Microbiol 71:4203-13. 2005
    ..A broad spectrum of new Verticillium antagonists was identified, and the implications of the data for biocontrol applications are discussed...
  22. ncbi request reprint A new semi-nested PCR protocol to amplify large 18S rRNA gene fragments for PCR-DGGE analysis of soil fungal communities
    Miruna Oros-Sichler
    Biologische Bundesanstalt für Land und Forstwirtschaft BBA, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11 12, 38104 Braunschweig, Germany
    J Microbiol Methods 65:63-75. 2006
    ..The semi-nested PCR system developed in this study, coupled with DGGE fingerprinting, offers a robust, reliable and sensitive tool for the analysis of soil fungal community structure...
  23. ncbi request reprint Survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community
    Monika Götz
    Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, Braunschweig, Germany
    FEMS Microbiol Ecol 56:207-18. 2006
    ..The colonization patterns observed by CLSM provide important information on the sampling strategy required for monitoring inoculant strains in the rhizosphere...
  24. doi request reprint Variation in permissiveness for broad-host-range plasmids among genetically indistinguishable isolates of Dickeya sp. from a small field plot
    Holger Heuer
    Julius Kühn Institut, Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11 12, Braunschweig, Germany
    FEMS Microbiol Ecol 73:190-6. 2010
    ..Such strains were isolated in close vicinity and from different plots of the field, indicating a reasonably fast genetic mechanism of switching between low and high permissiveness...
  25. ncbi request reprint Fungal endophytes in potato roots studied by traditional isolation and cultivation-independent DNA-based methods
    Monika Götz
    Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    FEMS Microbiol Ecol 58:404-13. 2006
    ..Differences in the relative abundance of endophytic fungi colonizing the roots of T4-lysozyme producing potatoes and the parental line could be detected by both methods...
  26. ncbi request reprint The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere
    Andreas Tauch
    Zentrum für Genomforschung, Universitat Bielefeld, D 33615 Bielefeld, Germany
    Microbiology 148:1637-53. 2002
    ..These data add to the growing evidence that plasmids carry advantageous genes with as yet undefined functions in plant-associated communities...
  27. doi request reprint Patchy distribution of flexible genetic elements in bacterial populations mediates robustness to environmental uncertainty
    Holger Heuer
    Federal Research Centre for Cultivated Plants, Julius Kühn Institute, Braunschweig, Germany
    FEMS Microbiol Ecol 65:361-71. 2008
    ..Consequently, mechanisms that make a subpopulation conducive to specific conjugative plasmids may have evolved, which could explain the persistence of even cryptic plasmids that do not encode any traits...
  28. doi request reprint Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids
    Chu Thi Thanh Binh
    Julius Kühn Institute, Federal Research Centre for Cultivated Crops, Braunschweig, Germany
    FEMS Microbiol Ecol 66:25-37. 2008
    ..This study showed that 'field-scale' piggery manure is a reservoir of broad-host range plasmids conferring multiple antibiotic resistance genes...
  29. doi request reprint Exploring the diversity of bacterial communities in sediments of urban mangrove forests
    Newton C Marcial Gomes
    Julius Kühn Institute for Cultivated Crops, Messeweg, Braunschweig, Germany
    FEMS Microbiol Ecol 66:96-109. 2008
    ..Many dominant DGGE ribotypes showed low levels of sequence identity to known sequences, indicating a large untapped bacterial diversity in mangrove ecosystems...
  30. ncbi request reprint Significance test for comparing complex microbial community fingerprints using pairwise similarity measures
    Siegfried Kropf
    Institute for Biometry and Medical Informatics, Otto von Guericke University, Leipziger Str 44, Magdeburg 39120, Germany
    J Microbiol Methods 57:187-95. 2004
    ..An example is given for the comparison of bacterial soil communities, testing the effect of different crop plants. Each community was represented by amplified ribosomal gene fragments separated in a denaturing gradient gel...
  31. ncbi request reprint Community structure of actively growing bacterial populations in plant pathogen suppressive soil
    Karin Hjort
    Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, SE 750 07, Uppsala, Sweden
    Microb Ecol 53:399-413. 2007
    ..These results demonstrate the utility of microbiomics, or a combination of molecular approaches, for investigating the composition of complex microbial communities in soil...
  32. ncbi request reprint The rhizosphere effect on bacteria antagonistic towards the pathogenic fungus Verticillium differs depending on plant species and site
    Gabriele Berg
    Institute for Life Sciences, University of Rostock, Microbiology, Rostock, Germany
    FEMS Microbiol Ecol 56:250-61. 2006
    ..Antagonists of the genus Serratia were preferentially isolated from oilseed rape rhizosphere, with the exception of one site. For isolates of Pseudomonas and Serratia, plant-specific and site-specific genotypes were found...
  33. ncbi request reprint The rhizosphere: 'soil compartment influenced by the root'
    Kornelia Smalla
    FEMS Microbiol Ecol 56:165. 2006
  34. ncbi request reprint Molecular classification of IncP-9 naphthalene degradation plasmids
    Tatyana Yu Izmalkova
    G K Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation
    Plasmid 56:1-10. 2006
    ..Discovery of a large group of plasmid with unique nahR suggested that the regulatory loop may also represent a variable part of the pathway for catabolism of naphthalene in fluorescent Pseudomonas spp...
  35. pmc Diversity of IncP-9 plasmids of Pseudomonas
    Yanina R Sevastsyanovich
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Microbiology 154:2929-41. 2008
    ....
  36. ncbi request reprint Comparison of independent samples of high-dimensional data by pairwise distance measures
    Siegfried Kropf
    Institute of Biometry and Medical Informatics, Otto von Guericke University, Magdeburg, Germany
    Biom J 49:230-41. 2007
    ..As the number of possible permutations is small in very small samples, this might restrict the use of the test. Therefore, we propose an exact parametric small sample version of the test using randomly rotated samples...
  37. ncbi request reprint Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community
    Newton C M Gomes
    Federal Biological Research Centre for Agriculture and Forestry BBA, Braunschweig, Germany
    FEMS Microbiol Ecol 54:21-33. 2005
    ..Although the inoculant strain KT2442 (pNF142) showed good survival and expression of genes involved in naphthalene degradation, this study suggests that KT2442 (pNF142) suppressed the enrichment of indigenous naphthalene degraders...
  38. ncbi request reprint PCR detection of oxytetracycline resistance genes otr(A) and otr(B) in tetracycline-resistant streptomycete isolates from diverse habitats
    Theodora L Nikolakopoulou
    Department of Botany, Microbiology Group, National and Kapodistrian University of Athens, Faculty of Biology, Greece
    Curr Microbiol 51:211-6. 2005
    ..Our results indicated that the occurrence of otr(A) and otr(B) genes in natural environments was limited and that otr(B), in comparison to otr(A), seemed to be more common...
  39. doi request reprint Microbial community networks
    Kornelia Smalla
    FEMS Microbiol Ecol 66:1-2. 2008
  40. pmc Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants
    Gabriele Berg
    Department of Microbiology, Institute for Molecular Physiology and Biotechnology, University of Rostock, D 18055 Rostock, Germany
    Appl Environ Microbiol 68:3328-38. 2002
    ..Pantoea agglomerans) were mainly isolated from the rhizosphere of oilseed rape. For P. putida A and B plant-specific genotypes were observed, suggesting that these bacteria were specifically enriched in each rhizosphere...
  41. pmc Repeat domain diversity of avrBs3-like genes in Ralstonia solanacearum strains and association with host preferences in the field
    Holger Heuer
    Department of Plant Pathology, Nanjing Agricultural University, Weigang No 1, Nanjing, China
    Appl Environ Microbiol 73:4379-84. 2007
    ..A statistically significant association between the originating plant species and internal repeats of the gene was found. Sequences of repeats and variation between nearly clonal strains revealed evidence of frequent recombination...