Ute Resch-Genger

Summary

Affiliation: Federal Institute for Materials Research and Testing
Country: Germany

Publications

  1. doi request reprint Standardization of fluorescence measurements: criteria for the choice of suitable standards and approaches to fit-for-purpose calibration tools
    Ute Resch-Genger
    Federal Institute for Materials Research and Testing BAM, I 5 Bioanalytics, Richard Willstaetter Str 11, 12489 Berlin Adlershof, Germany
    Ann N Y Acad Sci 1130:35-43. 2008
  2. ncbi request reprint State-of-the art comparability of corrected emission spectra. 1. Spectral correction with physical transfer standards and spectral fluorescence standards by expert laboratories
    Ute Resch-Genger
    Division I 5, BAM, Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, D 12489 Berlin, Germany
    Anal Chem 84:3889-98. 2012
  3. ncbi request reprint State-of-the art comparability of corrected emission spectra. 2. Field laboratory assessment of calibration performance using spectral fluorescence standards
    Ute Resch-Genger
    Division I 5, BAM, Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, D 12489 Berlin, Germany
    Anal Chem 84:3899-907. 2012
  4. doi request reprint Quantum dots versus organic dyes as fluorescent labels
    Ute Resch-Genger
    Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, 12489 Berlin, Germany
    Nat Methods 5:763-75. 2008
  5. doi request reprint Scope and limitations of surface functional group quantification methods: exploratory study with poly(acrylic acid)-grafted micro- and nanoparticles
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstatter Strasse 11, D 12489 Berlin, Germany
    J Am Chem Soc 134:8268-76. 2012
  6. doi request reprint Suitable labels for molecular imaging--influence of dye structure and hydrophilicity on the spectroscopic properties of IgG conjugates
    Jutta Pauli
    BAM Bundesanstalt für Materialforschung und prüfung, OE I 5, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Bioconjug Chem 22:1298-308. 2011
  7. doi request reprint Near-infrared-emitting nanoparticles for lifetime-based multiplexed analysis and imaging of living cells
    Katrin Hoffmann
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Straße 11, 12489 Berlin, Germany
    ACS Nano 7:6674-84. 2013
  8. doi request reprint Target-specific nanoparticles containing a broad band emissive NIR dye for the sensitive detection and characterization of tumor development
    Thomas Behnke
    BAM Federal Institute for Materials Research and Testing, 1 10, Richard Willstätter Str 11, 12489 Berlin, Germany
    Biomaterials 34:160-70. 2013
  9. doi request reprint Quantification of surface functional groups on polymer microspheres by supramolecular host-guest interactions
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstätter Straße 11, D 12489 Berlin, Germany
    Chem Commun (Camb) 47:7842-4. 2011
  10. doi request reprint Stability and fluorescence quantum yield of CdSe-ZnS quantum dots--influence of the thickness of the ZnS shell
    Markus Grabolle
    Federal Institute for Materials Research and Testing, Department I 5, Richard Willstaetter Str 11, 12489 Berlin, Germany
    Ann N Y Acad Sci 1130:235-41. 2008

Collaborators

Detail Information

Publications32

  1. doi request reprint Standardization of fluorescence measurements: criteria for the choice of suitable standards and approaches to fit-for-purpose calibration tools
    Ute Resch-Genger
    Federal Institute for Materials Research and Testing BAM, I 5 Bioanalytics, Richard Willstaetter Str 11, 12489 Berlin Adlershof, Germany
    Ann N Y Acad Sci 1130:35-43. 2008
    ..Special emphasis is dedicated to liquid and solid chromophore-based fluorescence standards developed or currently tested by us...
  2. ncbi request reprint State-of-the art comparability of corrected emission spectra. 1. Spectral correction with physical transfer standards and spectral fluorescence standards by expert laboratories
    Ute Resch-Genger
    Division I 5, BAM, Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, D 12489 Berlin, Germany
    Anal Chem 84:3889-98. 2012
    ....
  3. ncbi request reprint State-of-the art comparability of corrected emission spectra. 2. Field laboratory assessment of calibration performance using spectral fluorescence standards
    Ute Resch-Genger
    Division I 5, BAM, Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, D 12489 Berlin, Germany
    Anal Chem 84:3899-907. 2012
    ..The excellent comparability of the measurements of the field laboratories also demonstrates the effectiveness of RM-based correction procedures...
  4. doi request reprint Quantum dots versus organic dyes as fluorescent labels
    Ute Resch-Genger
    Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, 12489 Berlin, Germany
    Nat Methods 5:763-75. 2008
    ..Our aim is to provide a better understanding of the advantages and limitations of both classes of chromophores, to facilitate label choice and to address future challenges in the rational design and manipulation of QD labels...
  5. doi request reprint Scope and limitations of surface functional group quantification methods: exploratory study with poly(acrylic acid)-grafted micro- and nanoparticles
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstatter Strasse 11, D 12489 Berlin, Germany
    J Am Chem Soc 134:8268-76. 2012
    ..Comparison with results from protein labeling with streptavidin suggests a porous network of poly(acrylic acid) chains on the particle surface, which allows diffusion of small molecules (cutoff between 1.6 and 6.5 nm) into the network...
  6. doi request reprint Suitable labels for molecular imaging--influence of dye structure and hydrophilicity on the spectroscopic properties of IgG conjugates
    Jutta Pauli
    BAM Bundesanstalt für Materialforschung und prüfung, OE I 5, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Bioconjug Chem 22:1298-308. 2011
    ..20 in PBS and 0.10 for moderate dye-to-protein ratio of 2.5, a low aggregation tendency, and a superior reactivity in IgG labeling, we identified a promising diagnostic tool for the design of NIR fluorescent probes and protein conjugates...
  7. doi request reprint Near-infrared-emitting nanoparticles for lifetime-based multiplexed analysis and imaging of living cells
    Katrin Hoffmann
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Straße 11, 12489 Berlin, Germany
    ACS Nano 7:6674-84. 2013
    ..These proof-of-concept measurements underline the potential of fluorescent nanoparticle reporters in fluorescence lifetime multiplexing, barcoding, and imaging for cellular studies, cell-based assays, and molecular imaging. ..
  8. doi request reprint Target-specific nanoparticles containing a broad band emissive NIR dye for the sensitive detection and characterization of tumor development
    Thomas Behnke
    BAM Federal Institute for Materials Research and Testing, 1 10, Richard Willstätter Str 11, 12489 Berlin, Germany
    Biomaterials 34:160-70. 2013
    ..g. the sensitive detection and characterization of tumor development and progression...
  9. doi request reprint Quantification of surface functional groups on polymer microspheres by supramolecular host-guest interactions
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstätter Straße 11, D 12489 Berlin, Germany
    Chem Commun (Camb) 47:7842-4. 2011
    ..After centrifugation, CB7 in the supernatant is quantified by addition of a fluorescent dye. The difference between added and detected CB7 affords the number of accessible surface functional groups...
  10. doi request reprint Stability and fluorescence quantum yield of CdSe-ZnS quantum dots--influence of the thickness of the ZnS shell
    Markus Grabolle
    Federal Institute for Materials Research and Testing, Department I 5, Richard Willstaetter Str 11, 12489 Berlin, Germany
    Ann N Y Acad Sci 1130:235-41. 2008
    ..The quantum yield increases from less than 5% for unshelled CdSe up to 50%, with an increase in ZnS shell thickness up to 0.6-0.8 nm. At the same time, the particles become significantly more stable, as revealed by the shell test...
  11. doi request reprint Determination of the labeling density of fluorophore-biomolecule conjugates with absorption spectroscopy
    Markus Grabolle
    BAM Federal Institute for Materials Research and Testing, Division Biophotonic, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Bioconjug Chem 23:287-92. 2012
    ..This approach is illustrated for three different cyanine dyes conjugated to the antibody IgG...
  12. ncbi request reprint Polymer-and glass-based fluorescence standards for the near infrared (NIR) spectral region
    Christian Würth
    BAM Federal Institute for Materials Research and Testing I 5, Richard Willstaetter Str 11, 12489 Berlin, Germany
    J Fluoresc 21:953-61. 2011
    ..g. from spectrofluorometers over fluorescence microscopes to miniaturized fluorescence sensors...
  13. doi request reprint Novel fluorophores as building blocks for optical probes for in vivo near infrared fluorescence (NIRF) imaging
    Jutta Pauli
    BAM Federal Institute for Materials Research and Testing, Division I 5, Richard Willstaetter Str 11, 12489 Berlin, Germany
    J Fluoresc 20:681-93. 2010
    ..5 in PBS containing 5 mass-% bovine serum albumin (BSA) were comparable. Labeling experiments with DY-681 and the model antibody IgG revealed promisingly high Phi(f) values of the bioconjugated dye...
  14. ncbi request reprint Monitoring of amino functionalities on plasma-chemically modified polypropylene supports with a chromogenic and fluorogenic pyrylium reporter
    Katrin Hoffmann
    Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Langmuir 23:8411-6. 2007
    ..With this label, for the first time, plasma-chemically introduced amino functionalities could be monitored on the surface of a polymer film employing fluorescence spectroscopy and confocal laser scanning microscopy...
  15. doi request reprint New fluorescent labels with tunable hydrophilicity for the rational design of bright optical probes for molecular imaging
    Jutta Pauli
    BAM Federal Institute for Materials Research and Testing, Division 1 10 Biophotonics, Richard Willstaetter Strasse 11, D 12489 Berlin, Germany
    Bioconjug Chem 24:1174-85. 2013
    ..Moreover, we could demonstrate the potential of fluorescence spectroscopy to predict the size of fluorescence signals resulting for other fluorescence techniques such as FACS. ..
  16. doi request reprint Acoustically levitated droplets: a contactless sampling method for fluorescence studies
    Jork Leiterer
    Federal Institute for Materials Research and Testing BAM, Richard Willstätter Str 11, 12489 Berlin, Germany
    Ann N Y Acad Sci 1130:78-84. 2008
    ..The evaporation of the solvent during levitation leads to a continuous increase of solute concentration and can easily be monitored by laser-induced fluorescence...
  17. ncbi request reprint Biomembrane interactions of functionalized cryptophane-A: combined fluorescence and 129Xe NMR studies of a bimodal contrast agent
    Jagoda Sloniec
    Division 1 10 Biophotonics, BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, 12489 Berlin, Germany
    Chemistry 19:3110-8. 2013
    ..The results serve as a basis to differentiate biomembranes by analyzing the Hyper-CEST signatures that are related to membrane fluidity, and pave the way for dissecting different contributions to the Hyper-CEST signal...
  18. doi request reprint Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared
    Christian Würth
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Strasse 11, 12489 Berlin, Germany
    Anal Chem 84:1345-52. 2012
    ..Additionally, the absolute Φ(f) values of some red dyes that are among the most commonly used labels in the life sciences are presented as well as the absolute quantum yield of an optical probe for tumor imaging...
  19. ncbi request reprint Rigidization, preorientation and electronic decoupling--the 'magic triangle' for the design of highly efficient fluorescent sensors and switches
    Knut Rurack
    Federal Institute for Materials Research and Testing BAM, Richard Willstaetter Str 11, D 12489, Berlin, Germany
    Chem Soc Rev 31:116-27. 2002
    ..The examples comprise fluorescence 'OFF'-'ON' as well as 'ON'-'OFF' operative systems and the mechanisms, properties, and limitations of the different design concepts are discussed...
  20. doi request reprint Toward improved biochips based on rolling circle amplification--influences of the microenvironment on the fluorescence properties of labeled DNA oligonucleotides
    Elke Mayer-Enthart
    Federal Institute for Materials Research and Testing BAM, I 5 Bioanalytics, Richard Willstätter Str 11, 12489 Berlin, Germany
    Ann N Y Acad Sci 1130:287-92. 2008
    ..Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study...
  21. doi request reprint Fluorescence measurements on functionalized polymer surfaces--problems and troubleshooting
    Katrin Hoffmann
    Federal Institute for Materials Research and Testing BAM I 5 Bioanalytics, Richard Willstätter Str 11, D 12489 Berlin Adlershof, Germany
    Ann N Y Acad Sci 1130:28-34. 2008
    ....
  22. ncbi request reprint Nanoparticle-encapsulated vis- and NIR-emissive fluorophores with different fluorescence decay kinetics for lifetime multiplexing
    Katrin Hoffmann
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, 12489, Berlin, Germany
    Anal Bioanal Chem 406:3315-22. 2014
    ..g., for the design of labels and targeted probes for fluorescence assays and molecular imaging, cellular imaging studies, and barcoding applications, also in conjunction with spectral and intensity encoding. ..
  23. doi request reprint Excitation energy migration and trapping on the surface of fluorescent poly(acrylic acid)-grafted polymer particles
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstatter Strasse 11, D 12489 Berlin, Germany
    Photochem Photobiol Sci 12:729-37. 2013
    ..Furthermore, we suggest that this mechanism also applies to fluorescent protein conjugates and rationalizes the nonlinear dependence of the fluorescence emission on the labeling density...
  24. doi request reprint Nile-Red-nanoclay hybrids: red emissive optical probes for use in aqueous dispersion
    Tom Felbeck
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, 12489 Berlin, Germany
    Langmuir 29:11489-97. 2013
    ....
  25. doi request reprint Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29
    Lena Linck
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Eur J Med Chem 45:5561-6. 2010
    ..With the rhodamine DY-555, that is three times more emissive than Cy3, we found a bright and stable chromophore, the spectroscopic properties of which are minimally influenced by dye microenvironment...
  26. doi request reprint Photophysics and release kinetics of enzyme-activatable optical probes based on H-dimerized fluorophores on self-immolative linkers
    Jagoda Sloniec
    BAM Federal Institute for Materials Research and Testing, D 12489 Berlin, Germany
    J Phys Chem B 117:14336-44. 2013
    ..A kinetic analysis indicated that the fluorescence progress curves can be used to qualitatively extract enzyme kinetic parameters. ..
  27. doi request reprint An in vitro characterization study of new near infrared dyes for molecular imaging
    Jutta Pauli
    BAM Bundesanstalt fur Materialforschung und prufung, OE I 5, Richard Willstaetter Str 11, D 12489 Berlin, Germany
    Eur J Med Chem 44:3496-503. 2009
    ..With DY-681 and far-red excitable DY-731 and DY-751, we identified three dyes with improved properties compared to DY-676 and ICG...
  28. doi request reprint Anchoring of fluorophores to plasma-chemically modified polymer surfaces and the effect of cucurbit[6]uril on dye emission
    Katrin Hoffmann
    Bundesanstalt für Materialforschung und prüfung, Richard Willstaetter Str 11, 12489, Berlin, Germany
    J Fluoresc 19:229-37. 2009
    ..The observed effect is most likely due to CB6-induced rigidization of the linker molecules which seems to reduce fluorescence quenching dye-dye and fluorophore-surface interactions...
  29. doi request reprint Spectroscopic characterization of coumarin-stained beads: quantification of the number of fluorophores per particle with solid-state 19F-NMR and measurement of absolute fluorescence quantum yields
    Alexandra Huber
    Structural Analysis Division, BAM Federal Institute for Materials Research and Testing, Berlin, Germany
    Anal Chem 84:3654-61. 2012
    ....
  30. doi request reprint Simple colorimetric method for quantification of surface carboxy groups on polymer particles
    Andreas Hennig
    BAM Federal Institute for Materials Research and Testing, Richard Willstatter Strasse 11, D 12489 Berlin, Germany
    Anal Chem 83:4970-4. 2011
    ..g., for quality control by manufacturers of such materials...
  31. ncbi request reprint Encapsulation of hydrophobic dyes in polystyrene micro- and nanoparticles via swelling procedures
    Thomas Behnke
    BAM Federal Institute for Materials Research and Testing, Richard Willstaetter Str 11, 12489 Berlin, Germany
    J Fluoresc 21:937-44. 2011
    ..The main criterion for the optimization of the dye loading procedures was a high and thermally and photochemically stable fluorescence output of the particles for the future application of these systems as fluorescent labels...
  32. doi request reprint In vivo near-infrared fluorescence imaging of carcinoembryonic antigen-expressing tumor cells in mice
    Marcus René Lisy
    Institute for Diagnostic and Interventional Radiology, Friedrich Schiller University Jena, FZL Erlanger Allee 101, D 07747 Jena, Germany
    Radiology 247:779-87. 2008
    ..To prospectively depict carcinoembryonic antigen (CEA)-expressing tumors in mice with a high-affinity probe consisting of a near-infrared (NIR) fluorochrome and the clinically used anti-CEA antibody fragment arcitumomab...