Jeremy C Simpson

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. pmc Biogenesis of tubular ER-to-Golgi transport intermediates
    Jeremy C Simpson
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 17:723-37. 2006
  2. pmc The subcellular localization of the mammalian proteome comes a fraction closer
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 7:222. 2006
  3. ncbi request reprint A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
    Tobias Stauber
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 16:2245-51. 2006
  4. pmc Maturation of the mammalian secretome
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Genome Biol 8:211. 2007
  5. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
  6. pmc Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
    Veronika E Neubrand
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    EMBO J 24:1122-33. 2005
  7. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
  8. ncbi request reprint Imaging platforms for measurement of membrane trafficking
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:8-18. 2005
  9. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
  10. pmc High-content microscopy identifies new neurite outgrowth regulators
    Vibor Laketa
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory Heidelberg, 69117 Heidelberg, Germany
    Mol Biol Cell 18:242-52. 2007

Collaborators

Detail Information

Publications32

  1. pmc Biogenesis of tubular ER-to-Golgi transport intermediates
    Jeremy C Simpson
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 17:723-37. 2006
    ....
  2. pmc The subcellular localization of the mammalian proteome comes a fraction closer
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 7:222. 2006
    ..Here we discuss this new work in the context of other strategies for large-scale subcellular localization...
  3. ncbi request reprint A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
    Tobias Stauber
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 16:2245-51. 2006
    ..Our data strongly support a role for kinesin-2 in the KDEL-R-/COPI-dependent retrograde transport pathway from the Golgi complex to the ER...
  4. pmc Maturation of the mammalian secretome
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Genome Biol 8:211. 2007
    ....
  5. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
    ..Correlating these findings with other screens and bioinformatic information makes these candidates highly likely to be novel membrane traffic machinery components...
  6. pmc Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
    Veronika E Neubrand
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    EMBO J 24:1122-33. 2005
    ..This suggests that the direct interaction of 2c18 and gamma1-adaptin is crucial for membrane association and thus the function of the AP-1 complex in living cells. We propose to name this protein gamma-BAR...
  7. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
    ..It will also serve as an example for similar microscope-based screens addressing different biological questions...
  8. ncbi request reprint Imaging platforms for measurement of membrane trafficking
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:8-18. 2005
    ..We further describe the use of software macros and plugins in Image J that allow the quantification of the kinetics of membrane transport intermediates in fluorescence microscopy time-lapse sequences...
  9. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
    ..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
  10. pmc High-content microscopy identifies new neurite outgrowth regulators
    Vibor Laketa
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory Heidelberg, 69117 Heidelberg, Germany
    Mol Biol Cell 18:242-52. 2007
    ..Altogether, we demonstrate the potential of the systematic microscope-based screening approaches described here to tackle the complex biological process of neurite outgrowth regulation...
  11. ncbi request reprint siRNA cell arrays for high-content screening microscopy
    Holger Erfle
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 37:454-8, 460, 462. 2004
    ..We demonstrate such an siRNA cell array in a microscope-based functional assay in living cells to determine the effect of various siRNA oligonucleotides against endogenous targets on cellular secretion...
  12. pmc Spatiotemporal dynamics of the COPI vesicle machinery
    Markus Elsner
    Cell Biology and Cell Biophysics Programme, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 4:1000-4. 2003
    ..The addition of aluminium fluoride locks coatomer onto Golgi membranes and also decreases the binding kinetics of both ARF1 and ARFGAP1, suggesting that these proteins function in concert to mediate sorting and vesicle formation...
  13. doi request reprint A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells
    Carolina Tängemo
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Cell Sci 124:978-87. 2011
    ..Altogether, our data provide evidence that de novo Golgi biogenesis can occur in mammalian cells...
  14. ncbi request reprint Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle
    Dorit Arlt
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Cancer Res 65:7733-42. 2005
    ..We have established a novel functional profiling strategy that links genomics to cell biology and showed its potential for discerning cancer relevant modulators of the cell cycle in the candidate lists from microarray studies...
  15. pmc Localizing the proteome
    Jeremy C Simpson
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 4:240. 2003
    ..Comparison with less comprehensive studies of mammalian cells provides insights into the localization of the mammalian proteome...
  16. ncbi request reprint A role for the small GTPase Rab21 in the early endocytic pathway
    Jeremy C Simpson
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    J Cell Sci 117:6297-311. 2004
    ..Collectively, our data provide the first characterisation of Rab21 function in early endosome dynamics...
  17. pmc Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation
    Thomas Schallus
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 19:3404-14. 2008
    ..The localization, structure, and binding specificity of this protein, which we have named malectin, open the way to studies of its role in the genesis, processing and secretion of N-glycosylated proteins...
  18. ncbi request reprint Impact of live cell imaging on coated vesicle research
    Els Wessels
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Semin Cell Dev Biol 18:412-23. 2007
    ..Here, we present an overview of live cell-imaging experiments that have been used in the study of coated-vesicle transport, and provide specific examples of their impact on our understanding of coat function...
  19. doi request reprint ojoplano-mediated basal constriction is essential for optic cup morphogenesis
    Juan Ramon Martinez-Morales
    Developmental Unit, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 136:2165-75. 2009
    ..We propose a model of retinal morphogenesis whereby opo-mediated formation of focal contacts is required to transmit the mechanical tensions that drive the macroscopic folding of the vertebrate optic cup...
  20. pmc Navigating the secretory pathway: conference on exocytosis membrane structure and dynamics
    Emmanuel G Reynaud
    Cell Biology and Biophysics Programme, European Microbiology Laboratory, Heidelberg, Germany
    EMBO Rep 3:828-33. 2002
  21. ncbi request reprint Interferon-induced, antiviral human MxA protein localizes to a distinct subcompartment of the smooth endoplasmic reticulum
    Silke Stertz
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, D 79008 Freiburg, Germany
    J Interferon Cytokine Res 26:650-60. 2006
    ..The present results demonstrate that MxA is an IFN-induced antiviral effector protein that resembles the constitutively expressed large GTPase family members in its capacity to localize to and reorganize intracellular membranes...
  22. ncbi request reprint Cytoglobin is a respiratory protein in connective tissue and neurons, which is up-regulated by hypoxia
    Marc Schmidt
    Institute of Zoology, Johannes Gutenberg University, D 55099 Mainz, Germany
    J Biol Chem 279:8063-9. 2004
    ..Surprisingly, cytoglobin is localized in both the cytoplasm and nucleus of neurons, indicating a possible additional role of this protein in neuronal tissues...
  23. doi request reprint Rab18 and Rab43 have key roles in ER-Golgi trafficking
    Selma Y Dejgaard
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A2B2
    J Cell Sci 121:2768-81. 2008
    ..Rab18-S22N also potentiated brefeldin-A-induced ER-Golgi fusion. This study is the first comprehensive application of large-scale proteomics to the cell biology of small GTPases of the secretory pathway...
  24. ncbi request reprint Automated recognition system to classify subcellular protein localizations in images of different cell lines acquired by different imaging systems
    Yuh Show Tsai
    Department of Biomedical Engineering, Chung Yuan Christian University, Jhongli, Taiwan, Republic of China
    Microsc Res Tech 71:305-14. 2008
    ..In summary, we have established a single system that can recognize subcellular protein localizations from two different sources for location-proteomic studies. studies...
  25. ncbi request reprint Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Ther 10:1011-22. 2004
    ..The above observations suggest that penetratin and R8 peptides have distinct internalization mechanisms...
  26. ncbi request reprint Intracellular traffic and fate of protein transduction domains HIV-1 TAT peptide and octaarginine. Implications for their utilization as drug delivery vectors
    Saly Al-Taei
    Welsh School of Pharmacy, Cardiff University, Cardiff, CF10 3XF, Wales, United Kingdom
    Bioconjug Chem 17:90-100. 2006
    ..Disruption of Golgi and endoplasmic reticulum dynamics was without effect on peptide localization, suggesting that endosomes and lysosomes rather than these organelles are the major acceptor compartments for these molecules...
  27. ncbi request reprint Boosting multiclass learning with repeating codes and weak detectors for protein subcellular localization
    Chung Chih Lin
    Faculty of Life Sciences and Institute of Genomes, National Yang Ming University, Taipei, Taiwan
    Bioinformatics 23:3374-81. 2007
    ..One trend is the advances in multiclass learning with error-correcting output codes (ECOC). Another trend is the use of a large number of weak detectors with boosting for detecting objects in images of real-world scenes...
  28. pmc The systematic functional characterisation of Xq28 genes prioritises candidate disease genes
    Anja Kolb-Kokocinski
    Division of Molecular Genome Analysis, German Cancer Research Centre DKFZ, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    BMC Genomics 7:29. 2006
    ..To expedite disease gene identification, we have initiated the functional characterisation of all known Xq28 genes...
  29. ncbi request reprint RNA bacteriophage capsid-mediated drug delivery and epitope presentation
    William L Brown
    Ashbury Centre for Structural Molecular Biology, University of Leeds, UK
    Intervirology 45:371-80. 2002
    ..To use our knowledge of the three-dimensional structure and self-assembly mechanism of RNA bacteriophage capsids to develop novel virus-like particles (VLPs) for drug delivery and epitope presentation...
  30. ncbi request reprint Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells
    Sven Becker
    Institute for Transfusion Medicine and Immunohematology, Red Cross Blood Donor Service Baden Wuerttemberg Hesse, Sandhofstr 1, D 60528 Frankfurt, Germany
    Thromb Haemost 92:23-35. 2004
    ..Our data provide new details about the secretory route of FVIII, which may ultimately help to identify factors currently limiting the efficient and physiological expression of FVIII in gene therapy and manufacture...
  31. ncbi request reprint LETM1, a gene deleted in Wolf-Hirschhorn syndrome, encodes an evolutionarily conserved mitochondrial protein
    Stephanie Schlickum
    Institute of Human Genetics, Friedrich Alexander University Erlangen Nuremberg, Schwabachanlage 10, D 91054 Erlangen, Germany
    Genomics 83:254-61. 2004
    ..The present study presents information about a possible function for LETM1 and suggests that at least some (neuromuscular) features of WHS may be caused by mitochondrial dysfunction...
  32. ncbi request reprint Association of CD2AP with dynamic actin on vesicles in podocytes
    Thilo Welsch
    Department of Anatomy and Cell Biology, University of Heidelberg, D 69120 Heidelberg, Germany
    Am J Physiol Renal Physiol 289:F1134-43. 2005
    ....