Carsten Schultz

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint Prodrugs of biologically active phosphate esters
    Carsten Schultz
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117, Heidelberg, Germany
    Bioorg Med Chem 11:885-98. 2003
  2. ncbi request reprint Multiparameter imaging for the analysis of intracellular signaling
    Carsten Schultz
    European Molecular Biology Laboratory, Gene Expression Program, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Chembiochem 6:1323-30. 2005
  3. doi request reprint Can we see PIP(3) and hydrogen peroxide with a single probe?
    Natalie M Mishina
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    Antioxid Redox Signal 17:505-12. 2012
  4. pmc Inositol pentakisphosphate isomers bind PH domains with varying specificity and inhibit phosphoinositide interactions
    Sean G Jackson
    Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, L8N 3Z5, Canada
    BMC Struct Biol 11:11. 2011
  5. ncbi request reprint Climbing to the next step: photosensitive protein labels
    Carsten Schultz
    European Molecular Biology Laboratory, Heidelberg, Germany
    ACS Chem Biol 5:447-8. 2010
  6. ncbi request reprint Simultaneous protein tagging in two colors
    Carsten Schultz
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Chem Biol 15:91-2. 2008
  7. ncbi request reprint Fluorescent revelations
    Carsten Schultz
    European Molecular Biology Laboratory, Cell Biology and Biophysical Instrumentation Unit, Meyerhofstr 1, 69117 Heidelberg, Germany
    Chem Biol 16:107-11. 2009
  8. ncbi request reprint A dual parameter FRET probe for measuring PKC and PKA activity in living cells
    Justin Brumbaugh
    European Molecular Biology Institute EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Am Chem Soc 128:24-5. 2006
  9. ncbi request reprint Single- and dual-parameter FRET kinase probes based on pleckstrin
    Justin Brumbaugh
    European Molecular Biology Institute, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Protoc 1:1044-55. 2006
  10. pmc Plasma membrane phosphoinositide balance regulates cell shape during Drosophila embryo morphogenesis
    Alessandra Reversi
    European Molecular Biology Laboratory EMBL Heidelberg, 69117 Heidelberg, Germany
    J Cell Biol 205:395-408. 2014

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Prodrugs of biologically active phosphate esters
    Carsten Schultz
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117, Heidelberg, Germany
    Bioorg Med Chem 11:885-98. 2003
    ..The stability and toxicology of the resulting prodrugs is discussed. Finally, this review tries to cover briefly some of the work that describes the usefulness and efficiency of the approach in various application areas...
  2. ncbi request reprint Multiparameter imaging for the analysis of intracellular signaling
    Carsten Schultz
    European Molecular Biology Laboratory, Gene Expression Program, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Chembiochem 6:1323-30. 2005
    ..Here, we discuss the possibilities and limitations provided by multiparameter monitoring of cellular events with fluorescent probes. Some novel examples of the use of fluorescent probes and multiparameter imaging are shown...
  3. doi request reprint Can we see PIP(3) and hydrogen peroxide with a single probe?
    Natalie M Mishina
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    Antioxid Redox Signal 17:505-12. 2012
    ..We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts...
  4. pmc Inositol pentakisphosphate isomers bind PH domains with varying specificity and inhibit phosphoinositide interactions
    Sean G Jackson
    Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, L8N 3Z5, Canada
    BMC Struct Biol 11:11. 2011
    ..Given the structural similarity of inositol phosphates we were interested in examining the specificity of PH domains towards the family of myo-inositol pentakisphosphate isomers...
  5. ncbi request reprint Climbing to the next step: photosensitive protein labels
    Carsten Schultz
    European Molecular Biology Laboratory, Heidelberg, Germany
    ACS Chem Biol 5:447-8. 2010
    ..Hence the labeled protein is easily and unambiguously trackable against a background of nonilluminated proteins from different locations...
  6. ncbi request reprint Simultaneous protein tagging in two colors
    Carsten Schultz
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Chem Biol 15:91-2. 2008
    ..In this issue of Chemistry & Biology, Gautier et al. introduce a fluorescent labeling procedure orthogonal to existing ones, enabling tagging of two different proteins in living cells...
  7. ncbi request reprint Fluorescent revelations
    Carsten Schultz
    European Molecular Biology Laboratory, Cell Biology and Biophysical Instrumentation Unit, Meyerhofstr 1, 69117 Heidelberg, Germany
    Chem Biol 16:107-11. 2009
    ....
  8. ncbi request reprint A dual parameter FRET probe for measuring PKC and PKA activity in living cells
    Justin Brumbaugh
    European Molecular Biology Institute EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Am Chem Soc 128:24-5. 2006
    ..Mutations of the phosphate-accepting amino acids of the PKC substrate yielded a probe that was sensitive to PKA activation alone...
  9. ncbi request reprint Single- and dual-parameter FRET kinase probes based on pleckstrin
    Justin Brumbaugh
    European Molecular Biology Institute, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Protoc 1:1044-55. 2006
    ..Protocols for fluorescence microscopy of the probes in living cells and image processing are also described. Probe preparation takes 7 d; microscopy and image processing take 2 h...
  10. pmc Plasma membrane phosphoinositide balance regulates cell shape during Drosophila embryo morphogenesis
    Alessandra Reversi
    European Molecular Biology Laboratory EMBL Heidelberg, 69117 Heidelberg, Germany
    J Cell Biol 205:395-408. 2014
    ..Collectively, these data support a model in which the ratio of PI(4,5)P2/PI(3,4,5)P3 coordinates actomyosin contractility and plasma membrane expansion during tissue morphogenesis, thus ensuring proper cell shape. ..
  11. ncbi request reprint A rapidly reversible chemical dimerizer system to study lipid signaling in living cells
    Suihan Feng
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg Germany
    Angew Chem Int Ed Engl 53:6720-3. 2014
    ....
  12. doi request reprint FluoQ: a tool for rapid analysis of multiparameter fluorescence imaging data applied to oscillatory events
    Frank Stein
    European Molecular Biology Laboratory EMBL, Cell Biology and Biophysics Unit, Meyerhofstraße 1, 69117 Heidelberg, Germany
    ACS Chem Biol 8:1862-8. 2013
    ..This indicates that ITPKA contributes to the regulation of intracellular calcium transients by limiting inositol trisphosphate levels. ..
  13. doi request reprint Chemical activators of protein phosphatase-1 induce calcium release inside intact cells
    Gregor Reither
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Chem Biol 20:1179-86. 2013
    ..Our data show that PDPs can be used as selective chemical genetics tools to study acute and long-term effects of PP1 activation in intact cells, and PDPs will therefore be valuable tools to study PP1 biology. ..
  14. ncbi request reprint Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth
    Vibor Laketa
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Chem Biol 16:1190-6. 2009
    ..This work establishes membrane-permeant phosphoinositides as powerful tools to study PI3K signaling and directly demonstrates that 3-phosphorylated phosphoinositides are instrumental for neurite initiation...
  15. ncbi request reprint Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B
    Ivan A Yudushkin
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Science 315:115-9. 2007
    ..Such regulation may be important for generating distinct cellular environments that permit RTK signal transduction and that mediate its eventual termination...
  16. ncbi request reprint Heterogeneity and timing of translocation and membrane-mediated assembly of different annexins
    Tatsiana Skrahina
    Gene Expression Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Exp Cell Res 314:1039-47. 2008
    ..The formation of homo- and heterogeneous annexin assemblies may play an important role when high increases in calcium occur, such as after disruption of the plasma membrane...
  17. pmc Probing phospholipase a(2) with fluorescent phospholipid substrates
    Oliver Wichmann
    European Molecular Biology Laboratorium, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Chembiochem 8:1555-69. 2007
    ..Surprisingly, membrane-permeant versions of the new sensors that contained double bonds, PENN2 and PENN3, were only cleaved to a minor extent in HeLa cells while the saturated form, PENN, was well accepted...
  18. doi request reprint Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
    Devaraj Subramanian
    European Molecular Biology Institute, Cell Biology and Biophysics Unit, Heidelberg, Germany
    Nat Chem Biol 6:324-6. 2010
    ..In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process...
  19. doi request reprint Selective fluorescence labeling of lipids in living cells
    Anne B Neef
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Angew Chem Int Ed Engl 48:1498-500. 2009
    ..Copper(I)-catalyzed and strain-promoted azide-alkyne cycloaddition reactions were used for their visualization (see schematic representation and fluorescence microscopic image)...
  20. doi request reprint PIP₃ induces the recycling of receptor tyrosine kinases
    Vibor Laketa
    1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstraße 1, 69117 Heidelberg, Germany
    Sci Signal 7:ra5. 2014
    ..Thus, PIP3 and polarity complex proteins regulate receptor tyrosine kinase trafficking, which may enhance cellular responsiveness to growth factors. ..
  21. doi request reprint Protein tango: the toolbox to capture interacting partners
    Anna Rutkowska
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Angew Chem Int Ed Engl 51:8166-76. 2012
    ..These techniques open many new doors and enable the dissection of complicated protein networks. Herein we describe which cross-linkers and inducers of dimerization are out there and how to make use of this great toolbox...
  22. doi request reprint Protein translocation as a tool: The current rapamycin story
    Mateusz Putyrski
    Cell Biology and Biophysics Unit, EMBL Heidelberg, 69117 Heidelberg, Germany
    FEBS Lett 586:2097-105. 2012
    ..A number of very recent developments such as photoactivatable derivatives make rapamycin an even more attractive tool for basic science...
  23. doi request reprint Contribution of fluorophores to protein kinase C FRET probe performance
    Christiane A Jost
    EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany, Fax
    Chembiochem 9:1379-84. 2008
  24. ncbi request reprint Genetically encoded FRET probe for PKC activity based on pleckstrin
    Andreas Schleifenbaum
    European Molecular Biology Institute, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Am Chem Soc 126:11786-7. 2004
    ..FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way...
  25. doi request reprint Switching heterotrimeric G protein subunits with a chemical dimerizer
    Mateusz Putyrski
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Chem Biol 18:1126-33. 2011
    ..The results demonstrate that inducible heterotrimeric G protein subunits will provide ways for dissecting G protein-coupled receptor signaling...
  26. doi request reprint Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
    Amanda Cobos-Correa
    1 Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany 2 Molecular Medicine Partnership Unit, European Molecular Biology Laboratory and University of Heidelberg, Heidelberg, Germany
    Nat Chem Biol 5:628-30. 2009
    ..In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease...
  27. doi request reprint FRET-based and other fluorescent proteinase probes
    Hai Yu Hu
    European Molecular Biology Laboratory EMBL, Cell Biology and Biophysics Unit, Heidelberg, Germany Sanofi Deutschland GmbH, Diabetes Division, R and D, Industriepark Hoechst, Frankfurt am Main, Germany
    Biotechnol J 9:266-81. 2014
    ..Probe design and probe targeting are described and fluorescent probe development for disease-relevant proteinases, including matrix-metalloproteinases, cathepsins, caspases, and other selected proteinases, is reviewed. ..
  28. doi request reprint The Fatty Acid composition of diacylglycerols determines local signaling patterns
    André Nadler
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg Germany
    Angew Chem Int Ed Engl 52:6330-4. 2013
    ..Surprisingly, this distribution patterns depend on the fatty acid composition of the lipid species...
  29. pmc CFTR regulates early pathogenesis of chronic obstructive lung disease in βENaC-overexpressing mice
    Bjarki Johannesson
    Department of Translational Pulmonology, Translational Lung Research Center Heidelberg, Member of the German Center for Lung Research, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 7:e44059. 2012
    ....
  30. doi request reprint A FlAsH-based cross-linker to study protein interactions in living cells
    Anna Rutkowska
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Angew Chem Int Ed Engl 50:12655-8. 2011
    ..This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture)...
  31. ncbi request reprint Cellular uptake of PNA--terpyridine conjugates and its enhancement by Zn2+ ions
    Andreas Füssl
    European Molecular Biology Laboratory EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Am Chem Soc 128:5986-7. 2006
    ..Cellular accumulation is further enhanced in the presence of extracellular Zn2+...
  32. pmc Annexin A4 self-association modulates general membrane protein mobility in living cells
    Alen Piljic
    Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 17:3318-28. 2006
    ..Our work provides mechanistic insight into how annexin A4 may regulate plasma membrane protein function...
  33. ncbi request reprint A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms
    Oliver Wichmann
    European Molecular Biology Laboratory, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Angew Chem Int Ed Engl 45:508-12. 2006
  34. ncbi request reprint Probing lipid- and drug-binding domains with fluorescent dyes
    Shannon L Black
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Bioorg Med Chem 16:1162-73. 2008
    ..These results give valuable insight into lipid and drug transport by proteins outside and inside cells...
  35. ncbi request reprint In Vivo imaging of mouse tumors by a lipidated cathepsin s substrate
    Hai Yu Hu
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg Germany Sanofi Deutschland GmbH, Diabetes Division, R and D, Industriepark Park Höchst, 65926 Frankfurt Germany
    Angew Chem Int Ed Engl 53:7669-73. 2014
    ..The homing principle by probe lipidation might also work for selective administration of cytotoxic compounds to specifically reduce tumor mass. ..
  36. doi request reprint The power of fluorogenic probes
    André Nadler
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Angew Chem Int Ed Engl 52:2408-10. 2013
    ..New fluorogenic xanthene derivatives from the Bertozzi research group are getting us closer to "magic bullet" dyes...
  37. doi request reprint Chemical biologists gather in Heidelberg
    Maja Köhn
    Gene Expression Unit, European Molecular Biology Laboratory, Meyerhofstasse 1, 69117 Heidelberg, Germany
    Nat Chem Biol 5:66-9. 2009
    ....
  38. pmc Conformational analysis of a genetically encoded FRET biosensor by SAXS
    Haydyn D T Mertens
    European Molecular Biology Laboratory EMBL, Hamburg Outstation, c o DESY, Hamburg, Germany
    Biophys J 102:2866-75. 2012
    ..It is demonstrated that the synergistic use of SAXS and FRET methods allows one to resolve the ambiguities arising due to the rotation of the sensor molecules and the flexibility of the probe...
  39. doi request reprint The ENaC-overexpressing mouse as a model of cystic fibrosis lung disease
    Zhe Zhou
    Division of Pediatric Pulmonology and Allergy and Cystic Fibrosis Center, Department of Pediatrics III, University of Heidelberg, Heidelberg, Germany
    J Cyst Fibros 10:S172-82. 2011
    ..Here, we summarize approaches that can be applied for studies of the complex in vivo pathogenesis and preclinical evaluation of novel therapeutic strategies in this model of CF lung disease...
  40. pmc Inositol polyphosphate derivative inhibits Na+ transport and improves fluid dynamics in cystic fibrosis airway epithelia
    Mark Moody
    Inologic Inc, 101 Elliot Ave West, Suite 100, Seattle, WA 98119, USA
    Am J Physiol Cell Physiol 289:C512-20. 2005
    ..controls. These findings support preclinical development of INO-4995 for CF treatment and demonstrate for the first time the therapeutic potential of inositol polyphosphate derivatives...
  41. ncbi request reprint Lipid-induced phenotypes
    Carsten Schultz
    Nat Chem Biol 2:396-8. 2006
  42. ncbi request reprint Investigation of the ligand spectrum of human sterol carrier protein 2 using a direct mass spectrometry assay
    Will A Stanley
    EMBL Hamburg, c o DESY, Notkestrasse 85, 22603 Hamburg, Germany
    Arch Biochem Biophys 461:50-8. 2007
    ..A fluorescence-competition assay using Nile Red butyric acid confirmed the mass spectrometric observations in solution. The physiological significance of the lack of LCFA-carnitine binding by SCP2 is discussed...
  43. doi request reprint Simultaneous recording of multiple cellular events by FRET
    Alen Piljic
    ACS Chem Biol 3:156-60. 2008
    ..This type of imaging will likely be important for high content cell analysis and screening efforts in the future...
  44. ncbi request reprint Antagonists of myo-inositol 3,4,5,6-tetrakisphosphate allow repeated epithelial chloride secretion
    Marco T Rudolf
    Institut fur Organische Chemie, Universitat Bremen, UFT, 28359 Bremen, Germany
    Bioorg Med Chem 11:3315-29. 2003
    ....
  45. doi request reprint Fluorescence and bioluminescence procedures for functional proteomics
    Anke Prinz
    University of Kassel, Department of Biochemistry, Kassel, Germany
    Proteomics 8:1179-96. 2008
    ..In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology...