Rainer Pepperkok

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint High-throughput RNAi screening by time-lapse imaging of live human cells
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Methods 3:385-90. 2006
  2. pmc Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization
    Emmanuel G Reynaud
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    BMC Biol 3:21. 2005
  3. pmc Integrating systems biology with clinical research
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 9:314. 2008
  4. ncbi request reprint Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells
    David J Stephens
    Cell Biology and Cell Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Cell Sci 115:1149-60. 2002
  5. pmc Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'
    Joanne Young
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 16:162-77. 2005
  6. ncbi request reprint Secretory cargo regulates the turnover of COPII subunits at single ER exit sites
    Rebecca Forster
    Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany
    Curr Biol 16:173-9. 2006
  7. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
  8. ncbi request reprint A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
    Tobias Stauber
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 16:2245-51. 2006
  9. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
  10. ncbi request reprint Imaging platforms for measurement of membrane trafficking
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:8-18. 2005

Detail Information

Publications60

  1. ncbi request reprint High-throughput RNAi screening by time-lapse imaging of live human cells
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Methods 3:385-90. 2006
    ..This modular platform is scalable and makes the power of time-lapse microscopy available for genome-wide RNAi screens...
  2. pmc Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization
    Emmanuel G Reynaud
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    BMC Biol 3:21. 2005
    ..Therefore, they are ideal tools for comparative genomic studies aimed at understanding how aspects of biological complexity such as cellular compartmentalization evolved...
  3. pmc Integrating systems biology with clinical research
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 9:314. 2008
    ..A report on the conference 'Systems Genomics 2008', Heidelberg, Germany, 2-3 May 2008...
  4. ncbi request reprint Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells
    David J Stephens
    Cell Biology and Cell Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Cell Sci 115:1149-60. 2002
    ..These data reveal the existence of an early COPI-dependent, pre-Golgi cargo sorting step in mammalian cells...
  5. pmc Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'
    Joanne Young
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 16:162-77. 2005
    ..Together, this suggests that a recycling pathway operates at the level of the trans-Golgi linking directly to the ER. This pathway would be the preferred route for both toxins and resident Golgi proteins...
  6. ncbi request reprint Secretory cargo regulates the turnover of COPII subunits at single ER exit sites
    Rebecca Forster
    Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany
    Curr Biol 16:173-9. 2006
    ..We conclude that secretory cargo retains the COPII complex on membranes, after Sar1p release has occurred, and prevents premature disassembly of COPII during cargo sorting and transport carrier formation...
  7. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
    ..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
  8. ncbi request reprint A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
    Tobias Stauber
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 16:2245-51. 2006
    ..Our data strongly support a role for kinesin-2 in the KDEL-R-/COPI-dependent retrograde transport pathway from the Golgi complex to the ER...
  9. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  10. ncbi request reprint Imaging platforms for measurement of membrane trafficking
    Rainer Pepperkok
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:8-18. 2005
    ..We further describe the use of software macros and plugins in Image J that allow the quantification of the kinetics of membrane transport intermediates in fluorescence microscopy time-lapse sequences...
  11. ncbi request reprint Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells
    Oliver J Gruss
    EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
    Nat Cell Biol 4:871-9. 2002
    ..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells...
  12. ncbi request reprint In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery
    Julien Colombelli
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Traffic 6:1093-102. 2005
    ..We extend the application of the method by demonstrating induced dynamic behavior of actin-stress fibers after severing. This new method enables the quantitative investigation of cytoskeleton dynamics in a local confinement...
  13. pmc Biogenesis of tubular ER-to-Golgi transport intermediates
    Jeremy C Simpson
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 17:723-37. 2006
    ....
  14. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
    ..Correlating these findings with other screens and bioinformatic information makes these candidates highly likely to be novel membrane traffic machinery components...
  15. ncbi request reprint [Laser nanosurgery in cell biology]
    Julien Colombelli
    Laboratoire Européen de Biologie Moléculaire, LEBM, Meyerhofstrasse 1, D 69117 Heidelberg, Allemagne
    Med Sci (Paris) 22:651-8. 2006
    ....
  16. ncbi request reprint High-throughput fluorescence microscopy for systems biology
    Rainer Pepperkok
    Cell Biology Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 7:690-6. 2006
    ..To enable these techniques to be applied to functional genomics experiments, fluorescence microscopy is making the transition to a quantitative and high-throughput technology...
  17. pmc The subcellular localization of the mammalian proteome comes a fraction closer
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 7:222. 2006
    ..Here we discuss this new work in the context of other strategies for large-scale subcellular localization...
  18. pmc High-content microscopy identifies new neurite outgrowth regulators
    Vibor Laketa
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory Heidelberg, 69117 Heidelberg, Germany
    Mol Biol Cell 18:242-52. 2007
    ..Altogether, we demonstrate the potential of the systematic microscope-based screening approaches described here to tackle the complex biological process of neurite outgrowth regulation...
  19. ncbi request reprint Reverse transfection on cell arrays for high content screening microscopy
    Holger Erfle
    MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Protoc 2:392-9. 2007
    ..Different human cell lines that have been successfully transfected using the protocol are presented here. The present protocol has been applied to two genome-wide siRNA screens addressing mitosis and constitutive protein secretion...
  20. pmc Maturation of the mammalian secretome
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Genome Biol 8:211. 2007
    ....
  21. doi request reprint Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging
    Thomas Walter
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    J Struct Biol 170:1-9. 2010
    ..We demonstrate the use of these methods applying them on a genome wide RNAi screen by time-lapse microscopy...
  22. pmc Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G
    Heiko Runz
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, EMBL, Heidelberg, Germany
    EMBO J 25:2953-65. 2006
    ..Consistent with this, membrane turnover of the COPII component Sec23p is delayed in sterol-depleted cells. Altogether, our results demonstrate the importance of sterol levels in COPII mediated ER-export...
  23. ncbi request reprint siRNA cell arrays for high-content screening microscopy
    Holger Erfle
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 37:454-8, 460, 462. 2004
    ..We demonstrate such an siRNA cell array in a microscope-based functional assay in living cells to determine the effect of various siRNA oligonucleotides against endogenous targets on cellular secretion...
  24. ncbi request reprint Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells
    Holger Erfle
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Mol Biol 360:155-61. 2007
    ..This method should facilitate drug target validation by small-interfering RNAs...
  25. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
    ..It will also serve as an example for similar microscope-based screens addressing different biological questions...
  26. ncbi request reprint Differential requirements for ts-O45-G and procollagen biosynthetic transport
    Vytaute Starkuviene
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Traffic 8:1035-51. 2007
    ....
  27. ncbi request reprint Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair
    Timo Zimmermann
    Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Meyerhofstr 1, Heidelberg, Germany
    FEBS Lett 531:245-9. 2002
    ..Moreover, GFP2 has its excitation maximum at 396 nm at which the YFP acceptor is excited only below the detection level and thus this FRET pair is ideal for applications involving sensitized emission...
  28. ncbi request reprint Spectral imaging and its applications in live cell microscopy
    Timo Zimmermann
    Advanced Light Microscopy Facility and Cell Biology Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstr 1, D 69117 Heidelberg, Germany
    FEBS Lett 546:87-92. 2003
    ..Here we discuss recent progress on the technical implementation of the method, its limitations and applications to the imaging of biological samples...
  29. ncbi request reprint Transfected cell microarrays: an efficient tool for high-throughput functional analysis
    Vytaute Starkuviene
    European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
    Expert Rev Proteomics 4:479-89. 2007
    ..These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level...
  30. ncbi request reprint 'Harvester': a fast meta search engine of human protein resources
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Bioinformatics 20:1962-3. 2004
    ..With Harvester it is now possible to compare and check the quality of different database entries and prediction algorithms on a single page. A feedback forum allows users to comment on Harvester and to report database inconsistencies...
  31. pmc Localizing the proteome
    Jeremy C Simpson
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 4:240. 2003
    ..Comparison with less comprehensive studies of mammalian cells provides insights into the localization of the mammalian proteome...
  32. ncbi request reprint Arrays of transfected mammalian cells for high content screening microscopy
    Holger Erfle
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:1-8. 2005
    ..The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel...
  33. doi request reprint Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth
    Vibor Laketa
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Chem Biol 16:1190-6. 2009
    ..This work establishes membrane-permeant phosphoinositides as powerful tools to study PI3K signaling and directly demonstrates that 3-phosphorylated phosphoinositides are instrumental for neurite initiation...
  34. doi request reprint ojoplano-mediated basal constriction is essential for optic cup morphogenesis
    Juan Ramon Martinez-Morales
    Developmental Unit, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 136:2165-75. 2009
    ..We propose a model of retinal morphogenesis whereby opo-mediated formation of focal contacts is required to transmit the mechanical tensions that drive the macroscopic folding of the vertebrate optic cup...
  35. ncbi request reprint Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources
    Urban Liebel
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:19-26. 2005
    ..The Harvester search engine works similar to Google, offering genome-wide ranked results at very high speed. Here we describe how to use this bioinformatic tool along with selected examples...
  36. ncbi request reprint A correlative light and electron microscopy method based on laser micropatterning and etching
    Julien Colombelli
    Cell Biology and Cell Biophysics Unit, EMBL, Heidelberg, Germany
    Methods Mol Biol 457:203-13. 2008
    ..Furthermore, the glass surface is laser-etched and upon fixation and flat embedding, the inverse ridge can be localized as well as the cell of interest, which is then processed for electron microscopy...
  37. pmc Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts
    Stephane Brunet
    Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg 69 117, Germany
    Mol Biol Cell 15:5318-28. 2004
    ..These and previous results suggest that under physiological conditions, TPX2 is essential for microtubule nucleation around chromatin and functions in a network of other molecules, some of which also are regulated by RanGTP...
  38. doi request reprint Human Proteinpedia enables sharing of human protein data
    Suresh Mathivanan
    Nat Biotechnol 26:164-7. 2008
  39. pmc Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection
    Ayanthi A Richards
    Institute for Molecular Bioscience, Center for Microscopy and Microanalysis, Queensland 4072, Australia
    Mol Biol Cell 13:1750-64. 2002
    ..Our results identify novel inhibitors of SV40 infection and show that SV40 requires COPI- and COPII-dependent transport steps for successful infection...
  40. doi request reprint Rab18 and Rab43 have key roles in ER-Golgi trafficking
    Selma Y Dejgaard
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A2B2
    J Cell Sci 121:2768-81. 2008
    ..Rab18-S22N also potentiated brefeldin-A-induced ER-Golgi fusion. This study is the first comprehensive application of large-scale proteomics to the cell biology of small GTPases of the secretory pathway...
  41. doi request reprint Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
    Holger Erfle
    Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
    J Biomol Screen 13:575-80. 2008
    ..We conclude that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays...
  42. ncbi request reprint The German cDNA network: cDNAs, functional genomics and proteomics
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    J Struct Funct Genomics 4:87-96. 2003
    ....
  43. pmc Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
    Veronika E Neubrand
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    EMBO J 24:1122-33. 2005
    ..This suggests that the direct interaction of 2c18 and gamma1-adaptin is crucial for membrane association and thus the function of the AP-1 complex in living cells. We propose to name this protein gamma-BAR...
  44. pmc LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system
    Detlev Bannasch
    German Cancer Research Center DKFZ, Division of Molecular Genome Analysis, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 32:D505-8. 2004
    ..A web interface enables the scientific community to explore and visualize features of the annotated cDNAs and ORFs combined with experimental results, and thus helps to unravel new features of proteins with as yet unknown functions...
  45. pmc From ORFeome to biology: a functional genomics pipeline
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg, Germany
    Genome Res 14:2136-44. 2004
    ..All information is presented in a Web database (http://www.dkfz.de/LIFEdb). It is exploited for the identification of disease-relevant genes and proteins for diagnosis and therapy...
  46. pmc Coupling of ER exit to microtubules through direct interaction of COPII with dynactin
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Nat Cell Biol 7:48-55. 2005
    ..Together, our data suggest a mechanism by which membranes of the early secretory pathway can be linked to motors and microtubules for subsequent organization and movement to the Golgi apparatus...
  47. pmc The LIFEdb database in 2006
    Alexander Mehrle
    Division Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 34:D415-8. 2006
    ..LIFEdb enables researchers to systematically select and characterize genes and proteins of interest, and presents data and information via its user-friendly web-based interface...
  48. ncbi request reprint Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 117:3635-44. 2004
    ..These data show a differential requirement for efficient GTP hydrolysis by the Sar1p GTPase in export of cargo from the ER...
  49. pmc Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites
    Yao Dong Yang
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Cell 17:1513-31. 2005
    ..By contrast, Golgi stacks associate intermittently and sometimes concurrently with several ERES as they move...
  50. ncbi request reprint Live imaging of bidirectional traffic from the ERGIC
    Houchaima Ben-Tekaya
    Department of Pharmacology and Neurobiology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    J Cell Sci 118:357-67. 2005
    ..These results suggest that the ERGIC is stationary and not simply a collection of mobile carriers that mediate protein traffic from endoplasmic reticulum to Golgi...
  51. pmc Automatic identification of subcellular phenotypes on human cell arrays
    Christian Conrad
    Intelligent Bioinformatics Systems, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Genome Res 14:1130-6. 2004
    ..Our classification method can be adapted to virtually any microscopic assay based on cell morphology, opening a wide range of applications including large-scale RNAi screening in human cells...
  52. ncbi request reprint Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells
    Heiko Runz
    Center for Molecular Biology, University of Heidelberg, 69120 Heidelberg, Germany
    J Neurosci 22:1679-89. 2002
    ..Our results suggest that the subcellular distribution of cholesterol may be an important factor in how cholesterol alters Abeta production and the risk of Alzheimer's disease...
  53. ncbi request reprint Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues
    Michael Keese
    Surgical Clinic, University Hospital Mannheim, D 68167 Mannheim, Germany
    J Biol Chem 280:27826-31. 2005
    ..Imaging of EGFR phosphorylation may thus serve as a valuable tool to investigate the role of receptor tyrosine kinase activity in malignant cell growth...
  54. ncbi request reprint Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions
    Shirley K Knauer
    Georg Speyer Haus, Paul Ehrlich Str 42 44, D 60596 Frankfurt, Germany
    Traffic 6:594-606. 2005
    ....
  55. ncbi request reprint Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells
    Olivier Tabary
    Inserm UMR 719, Universite Pierre et Marie Curie, Hopital Saint Antoine, Bât Kourilsky 184, rue du Fg Saint Antoine 75571, Paris, France
    Cell Signal 18:652-60. 2006
    ..Our results support the concept that [Ca(2+)](i) is a key regulator of NF-(kappa)B activation in CF airway epithelial cells...
  56. ncbi request reprint Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle
    Dorit Arlt
    Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany
    Cancer Res 65:7733-42. 2005
    ..We have established a novel functional profiling strategy that links genomics to cell biology and showed its potential for discerning cancer relevant modulators of the cell cycle in the candidate lists from microarray studies...
  57. ncbi request reprint CDNAs for functional genomics and proteomics: the German Consortium
    Stefan Wiemann
    Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    C R Biol 326:1003-9. 2003
    ..The resulting information is integrated with functional genomics and proteomics data for further understanding of protein functions in the cellular context...
  58. doi request reprint In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum
    François Pouthas
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    J Cell Sci 121:2406-14. 2008
    ..We propose that the positioning of the Golgi complex and the centrosome depends on the geometrical constraints applied to the cell rather than on a precise migratory function in the leading region...
  59. ncbi request reprint Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells
    Sven Becker
    Institute for Transfusion Medicine and Immunohematology, Red Cross Blood Donor Service Baden Wuerttemberg Hesse, Sandhofstr 1, D 60528 Frankfurt, Germany
    Thromb Haemost 92:23-35. 2004
    ..Our data provide new details about the secretory route of FVIII, which may ultimately help to identify factors currently limiting the efficient and physiological expression of FVIII in gene therapy and manufacture...
  60. ncbi request reprint Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion
    Stephane Brunet
    Biologie du Développement, CNRS UMR 7622 bat C 5ème, 9, quai St Bernard, 75252 Paris Cedex 05, France
    Traffic 7:1283-9. 2006
    ..It has the potential to be extended to study interactions of proteins or multi-protein complexes with different biopolymers like actin microfilaments or organelle membranes...