I W Mattaj

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. pmc An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B" spliceosome proteins
    M Hetzer
    European Molecular Biology Laboratory EMBL, D 69117 Heidelberg, Germany
    J Cell Biol 148:293-303. 2000
  2. ncbi request reprint Ribonucleoprotein assembly: clues from spinal muscular atrophy
    I W Mattaj
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Curr Biol 8:R93-5. 1998
  3. ncbi request reprint Nucleocytoplasmic transport: the soluble phase
    I W Mattaj
    European Molecular Biology Laboratory, Heidelberg, Germany
    Annu Rev Biochem 67:265-306. 1998
  4. ncbi request reprint Sorting out the nuclear envelope from the endoplasmic reticulum
    Iain W Mattaj
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 5:65-9. 2004
  5. pmc The evolutionarily conserved region of the U snRNA export mediator PHAX is a novel RNA-binding domain that is essential for U snRNA export
    A Segref
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 7:351-60. 2001
  6. ncbi request reprint PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation
    M Ohno
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 101:187-98. 2000
  7. pmc RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase
    P Askjaer
    Department of Gene Expression, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Mol Cell Biol 19:6276-85. 1999
  8. pmc Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition
    P Fortes
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:2425-38. 1999
  9. pmc The role of exportin-t in selective nuclear export of mature tRNAs
    G J Arts
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 17:7430-41. 1998
  10. ncbi request reprint A nuclear cap binding protein complex involved in pre-mRNA splicing
    E Izaurralde
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Cell 78:657-68. 1994

Collaborators

  • R E Carazo-Salas
  • C McGuigan
  • E Karsenti
  • M Hetzer
  • F R Bischoff
  • T Preiss
  • K Weis
  • J D Lewis
  • M Yoshida
  • T D Allen
  • U Kutay
  • M Etzerodt
  • C Niehrs
  • D Gorlich
  • D Tollervey
  • E Fabre
  • H H Meyer
  • C Wimmer
  • W C Boelens
  • C Kambach
  • Vincent Galy
  • Wolfram Antonin
  • M Fornerod
  • M Ohno
  • Matyas Gorjanacz
  • Sebastian Ulbert
  • Matthias Wilm
  • P Fortes
  • Peter Askjaer
  • Hideki Yokoyama
  • Oliver J Gruss
  • Katharina Ribbeck
  • Malgorzata Schelder
  • Melpomeni Platani
  • Jörg Mansfeld
  • G J Arts
  • A Segref
  • Uta Haselmann
  • Cerstin Franz
  • S Kuersten
  • Virginie Hachet
  • Thomas Kocher
  • Tobias C Walther
  • E Izaurralde
  • T C Walther
  • L Englmeier
  • M Polycarpou-Schwarz
  • M Wilm
  • C Gamberi
  • A Bachi
  • Sofia Rybina
  • P Askjaer
  • Maiwen Caudron
  • Rachel Santarella
  • Carmen Lopez-Iglesias
  • Andreas Jaedicke
  • Elke P F Klerkx
  • Tim Raemaekers
  • Geert Carmeliet
  • Richard W Wozniak
  • Stephanie Boue
  • Petra Mühlhäusser
  • Nelly Pante
  • Lisa A Hawryluk-Gara
  • Stephan Güttinger
  • Moritz Mall
  • Carmen López Iglesias
  • B Seraphin
  • Ario de Marco
  • Claude Antony
  • Bastian B Hülsmann
  • Isabelle Loiodice
  • Helen Pickersgill
  • Valérie Doye
  • Annabelle Alves
  • Eva Hannak
  • Rainer Pepperkok
  • Isabelle Vernos
  • Malte Wittmann
  • Herman Sillje
  • Thomas Kufer
  • H Pickersgill
  • M Goldberg
  • C Petosa
  • A B Sachs
  • T Inada
  • M W Hentze
  • G Rigaut
  • J Kjems
  • D Bilbao-Cortes

Detail Information

Publications46

  1. pmc An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B" spliceosome proteins
    M Hetzer
    European Molecular Biology Laboratory EMBL, D 69117 Heidelberg, Germany
    J Cell Biol 148:293-303. 2000
    ..This activity can be solubilized in the presence of elevated MgCl(2). These data suggest that U1A and U2B" import into the nucleus occurs by a hitherto uncharacterized mechanism...
  2. ncbi request reprint Ribonucleoprotein assembly: clues from spinal muscular atrophy
    I W Mattaj
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Curr Biol 8:R93-5. 1998
    ..The SMN protein has been found to be part of a multi-component complex that appears to function in the assembly of cellular ribonucleoprotein particles...
  3. ncbi request reprint Nucleocytoplasmic transport: the soluble phase
    I W Mattaj
    European Molecular Biology Laboratory, Heidelberg, Germany
    Annu Rev Biochem 67:265-306. 1998
    ..The Ran GTPase is critical in generating this asymmetry. Regulation of nucleocytoplasmic transport generally involves specific inhibition of the formation of a transport complex; however, more global forms of regulation also occur...
  4. ncbi request reprint Sorting out the nuclear envelope from the endoplasmic reticulum
    Iain W Mattaj
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 5:65-9. 2004
    ..In metazoans, the nuclear envelope breaks down during mitosis and is thought to be completely reabsorbed by the endoplasmic reticulum. How these near neighbours are sorted out at the end of mitosis is an interesting mystery...
  5. pmc The evolutionarily conserved region of the U snRNA export mediator PHAX is a novel RNA-binding domain that is essential for U snRNA export
    A Segref
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 7:351-60. 2001
    ..The interaction domain of PHAX with CBC is at least partly distinct from the RNA-binding domain and the NLSs. Thus, the different interaction domains of PHAX allow it to act as a scaffold for the assembly of U snRNA export complexes...
  6. ncbi request reprint PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation
    M Ohno
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 101:187-98. 2000
    ..PHAX phosphorylation is essential for export complex assembly while its dephosphorylation causes export complex disassembly. The compartmentalized PHAX phosphorylation cycle can contribute to the directionality of export...
  7. pmc RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase
    P Askjaer
    Department of Gene Expression, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Mol Cell Biol 19:6276-85. 1999
    ..In addition, we identify the Xenopus homologue of the nucleoporin CAN/Nup214 as a RanGTP- and NES cargo-specific binding site for CRM1, suggesting that this nucleoporin plays a role in export complex disassembly and/or CRM1 recycling...
  8. pmc Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition
    P Fortes
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:2425-38. 1999
    ..These data suggest that the loss of Luc7p disrupts U1 snRNP-CBC interaction, and that this interaction contributes to normal 5' splice site recognition...
  9. pmc The role of exportin-t in selective nuclear export of mature tRNAs
    G J Arts
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 17:7430-41. 1998
    ..These results suggest that at least two mechanisms are involved in discrimination of pre-tRNAs and mature tRNAs prior to nuclear export...
  10. ncbi request reprint A nuclear cap binding protein complex involved in pre-mRNA splicing
    E Izaurralde
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Cell 78:657-68. 1994
    ..Extracts immunodepleted of CBC do not efficiently splice an adenoviral pre-mRNA owing to blockage of an early step in splicing complex formation. CBC may therefore play a role in pre-mRNA recognition...
  11. pmc RanBP3 influences interactions between CRM1 and its nuclear protein export substrates
    L Englmeier
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 2:926-32. 2001
    ..These data indicate that RanBP3 functions by a novel mechanism as a cofactor in recognition and export of certain CRM1 substrates. In vitro, RanBP3 binding to CRM1 affects the relative affinity of CRM1 for different substrates...
  12. pmc Genetic and physical interactions involving the yeast nuclear cap-binding complex
    P Fortes
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Cell Biol 19:6543-53. 1999
    ..Mutants lacking yCBC were found to be defective in rRNA processing. Analysis of the yCBC deletion phenotype suggests that this is likely to be due to a defect in the splicing of a subset of ribosomal protein mRNA precursors...
  13. ncbi request reprint Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
    M Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 3:1086-91. 2001
    ..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation...
  14. pmc The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins
    T C Walther
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 20:5703-14. 2001
    ..Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events...
  15. pmc Interaction between the human nuclear cap-binding protein complex and hnRNP F
    C Gamberi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell Biol 17:2587-97. 1997
    ..Thus, hnRNP F is required for efficient pre-mRNA splicing in vitro and may participate in the effect of CBC on pre-mRNA splicing...
  16. ncbi request reprint The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation
    P Fortes
    Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell 6:191-6. 2000
    ..These data suggest that eIF4E binding to the eIF4G-CBC complex on newly exported mRNA displaces CBC, and that the first round of translation on mRNA may occur via a different mechanism than subsequent rounds...
  17. ncbi request reprint Nucleocytoplasmic transport: Ran, beta and beyond
    S Kuersten
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117, Heidelberg, Germany
    Trends Cell Biol 11:497-503. 2001
    ....
  18. ncbi request reprint CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
    ..We conclude that CRM1 is an export receptor for leucine-rich nuclear export signals and discuss a model for the role of RanGTP in CRM1 function and in nuclear export in general...
  19. ncbi request reprint Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly
    R E Carazo-Salas
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 3:228-34. 2001
    ..We also show that inhibition of Ran-GTP production, even in the presence of duplicated centrosomes and kinetochores, prevents assembly of a bipolar spindle in M-phase extracts...
  20. pmc A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing
    J D Lewis
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nucleic Acids Res 24:3332-6. 1996
    ..These results, together with other data, suggest that CBCs play a highly conserved role in the recognition of pre-mRNA substrates at an early step in the splicing process...
  21. ncbi request reprint Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation
    R E Carazo-Salas
    EMBL, Heidelberg, Germany
    Nature 400:178-81. 1999
    ..We propose that RCC1 generates a high local concentration of Ran-GTP around chromatin which in turn induces the local nucleation of microtubules...
  22. pmc Drosophila SNF/D25 combines the functions of the two snRNP proteins U1A and U2B' that are encoded separately in human, potato, and yeast
    M Polycarpou-Schwarz
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 2:11-23. 1996
    ..Thus, its RNA-binding properties are the sum of those exhibited by human or potato U1A and U2B' proteins. Implications for the role of SNF/D25 in alternative splicing, and for the evolution of the U1A/U2B' protein family, are discussed...
  23. ncbi request reprint Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences
    K Weis
    European Molecular Biology Laboratory, Heidelberg, Germany
    Science 268:1049-53. 1995
    ..Thus, hSRP1 alpha has the properties of a cytosolic receptor for both simple and bipartite NLS motifs...
  24. ncbi request reprint Identification of a nuclear export receptor for tRNA
    G J Arts
    European Molecular Biology Laboratory, Heidelberg, Germany
    Curr Biol 8:305-14. 1998
    ..Export complexes form and import complexes disassemble on binding of RanGTP to the receptor. Yeast Los 1 p is a member of the importin-beta family with a poorly defined role in tRNA production...
  25. ncbi request reprint Nucleocytoplasmic transport: the last 200 nanometers
    M Ohno
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 92:327-36. 1998
  26. ncbi request reprint A cap-binding protein complex mediating U snRNA export
    E Izaurralde
    European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    Nature 376:709-12. 1995
    ..These results demonstrate that CBC mediates the effect of the cap structure in U snRNA export, and provide direct evidence for the involvement of a cellular RNA-binding factor in the transport of RNA to the cytoplasm...
  27. ncbi request reprint Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif
    E Fabre
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Cell 78:275-89. 1994
    ..Fragments of Nup145p and Nup116p including this motif bind specifically to homopolymeric RNAs in vitro. Nup145p, Nup116p, and Nup100p thus represent a novel class of nucleoporins involved in nucleocytoplasmic transport...
  28. pmc Structure and expression of a Xenopus gene encoding an snRNP protein (U1 70K)
    M Etzerodt
    European Molecular Biology Laboratory, Heidelberg, FRG
    EMBO J 7:4311-21. 1988
    ..The promoter region of one of the cloned genes is demonstrated to be functionally active, and to show apparent differences from other pol II promoters...
  29. ncbi request reprint Conserved amino acid residues within and outside of the N-terminal ribonucleoprotein motif of U1A small nuclear ribonucleoprotein involved in U1 RNA binding
    D Scherly
    European Molecular Biology Laboratory, Heidelberg, Germany
    J Mol Biol 219:577-84. 1991
    ..These are extremely similar (94.4% identical) between amino acid residues 7 and 114 but much less conserved immediately upstream and downstream from this region...
  30. ncbi request reprint What can Caenorhabditis elegans tell us about the nuclear envelope?
    Matyas Gorjanacz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 581:2794-801. 2007
    ..In this review we discuss the molecular constituents of the Caenorhabditis elegans NE and describe their role in post-mitotic NE re-formation, as well as the usefulness of C. elegans as an in vivo system for analyzing NE dynamics...
  31. ncbi request reprint A role for NuSAP in linking microtubules to mitotic chromosomes
    Katharina Ribbeck
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Curr Biol 17:230-6. 2007
    ..We propose that the presence of MT binding agents such as NuSAP, which can be directly immobilized on chromatin, are critical for targeting MT production to vertebrate chromosomes during spindle self-organization...
  32. pmc Caenorhabditis elegans BAF-1 and its kinase VRK-1 participate directly in post-mitotic nuclear envelope assembly
    Matyas Gorjanacz
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 26:132-43. 2007
    ..We propose that phosphorylation of BAF by VRK plays an essential regulatory role in the association of BAF with chromatin and nuclear membrane proteins during NE formation...
  33. ncbi request reprint The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology
    Sebastian Ulbert
    EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    FEBS Lett 580:6435-41. 2006
    ..We suggest that interactions of Lem2 with lamins or chromatin are critical for maintaining the integrity of the nuclear envelope...
  34. ncbi request reprint MEL-28, a novel nuclear-envelope and kinetochore protein essential for zygotic nuclear-envelope assembly in C. elegans
    Vincent Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 16:1748-56. 2006
    ..We show that MEL-28 is an early-assembling, stable NE component required for all aspects of NE assembly...
  35. pmc Direct membrane protein-DNA interactions required early in nuclear envelope assembly
    Sebastian Ulbert
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    J Cell Biol 173:469-76. 2006
    ..Membrane fractions that are essential for NE formation are shown to bind directly to protein-free DNA, and our data suggest that these interactions are critical for early steps in NE assembly...
  36. ncbi request reprint The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells
    Jörg Mansfeld
    Institute of Biochemistry, ETH Zurich, CH 8093 Zurich, Switzerland
    Mol Cell 22:93-103. 2006
    ..Importantly, NDC1 interacts with Nup53 in vitro. This suggests that NDC1 function involves forming a link between the NE membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane...
  37. ncbi request reprint Pushing the envelope: structure, function, and dynamics of the nuclear periphery
    Martin W Hetzer
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Cell Dev Biol 21:347-80. 2005
    ..Despite the importance of the NE for eukaryotic cell life, relatively little is known about its biogenesis or many of its functions. We thus are far from understanding the molecular etiology of a diverse group of NE-associated diseases...
  38. pmc Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates
    Cerstin Franz
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 24:3519-31. 2005
    ..Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs...
  39. ncbi request reprint The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation
    Wolfram Antonin
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Mol Cell 17:83-92. 2005
    ....
  40. pmc Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro
    Virginie Hachet
    European Molecular Biology Laboratory, Meyerhofstrasse, Heidelberg, Germany
    EMBO J 23:1526-35. 2004
    ..Unlike other functions of Importin alpha, this membrane-associated activity does not require interaction with NLS proteins...
  41. pmc Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo
    Vincent Galy
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 14:5104-15. 2003
    ..Thus, the contribution to NPC structure of Nup93 and Nup205 is essential for establishment of normal NPC function and for cell viability...
  42. ncbi request reprint The conserved Nup107-160 complex is critical for nuclear pore complex assembly
    Tobias C Walther
    EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 113:195-206. 2003
    ..Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex...
  43. pmc Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos
    Peter Askjaer
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 13:4355-70. 2002
    ..Finally, we have demonstrated that both IMA-2 and IMB-1, the homologues of vertebrate importin alpha and beta, are essential for both spindle assembly and nuclear formation in early embryos...
  44. ncbi request reprint Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells
    Oliver J Gruss
    EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
    Nat Cell Biol 4:871-9. 2002
    ..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells...
  45. ncbi request reprint The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly
    Martin Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 4:E177-84. 2002
    ..Ran functions in these events as a signal for chromosome position...
  46. pmc Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rate
    Hideki Yokoyama
    European Molecular Biology Laboratory, Heidelberg 69117, Germany
    J Cell Biol 180:867-75. 2008
    ..Our results indicate that Cdk11 is responsible for RanGTP-dependent MT stabilization around chromosomes and that this local stabilization is essential for normal rates of spindle assembly and spindle function...