Michael Knop

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. pmc Efficient protein depletion by genetically controlled deprotection of a dormant N-degron
    Christof Taxis
    EMBL, Cell Biology and Biophysics Unit, Meyerhofstr 1, Heidelberg, Germany
    Mol Syst Biol 5:267. 2009
  2. pmc Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
    Christof Taxis
    The European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    J Cell Biol 171:627-40. 2005
  3. pmc Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization
    Emmanuel G Reynaud
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    BMC Biol 3:21. 2005
  4. ncbi request reprint Evolution of the hemiascomycete yeasts: on life styles and the importance of inbreeding
    Michael Knop
    Cell Biology and Biophysics Unit, EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Bioessays 28:696-708. 2006
  5. pmc Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast
    Michael Knop
    EMBL, Heidelberg D 69117, Germany
    Mol Biol Cell 16:4543-56. 2005
  6. pmc Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane
    Peter Maier
    EMBL, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO J 26:1843-52. 2007
  7. pmc The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis
    Peter Maier
    EMBL, Cell Biology and Biophysics Unit, Heidelberg, Germany
    EMBO J 27:2363-74. 2008
  8. ncbi request reprint Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis
    Christian G Riedel
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    J Biol Chem 280:37846-52. 2005
  9. pmc Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy
    Romain Gibeaux
    European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genes Dev 27:335-49. 2013
  10. ncbi request reprint Tandem fluorescent protein timers for in vivo analysis of protein dynamics
    Anton Khmelinskii
    Center for Molecular Biology of the University of Heidelberg ZMBH, DKFZ ZMBH Alliance, Heidelberg, Germany
    Nat Biotechnol 30:708-14. 2012

Collaborators

Detail Information

Publications24

  1. pmc Efficient protein depletion by genetically controlled deprotection of a dormant N-degron
    Christof Taxis
    EMBL, Cell Biology and Biophysics Unit, Meyerhofstr 1, Heidelberg, Germany
    Mol Syst Biol 5:267. 2009
    ..The features of the TIPI system make it a versatile tool to study protein function in eukaryotes and to create new modules for synthetic or systems biology...
  2. pmc Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
    Christof Taxis
    The European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    J Cell Biol 171:627-40. 2005
    ..This is beneficial for the immediate fitness of the population of postmeiotic cells...
  3. pmc Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization
    Emmanuel G Reynaud
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    BMC Biol 3:21. 2005
    ..Therefore, they are ideal tools for comparative genomic studies aimed at understanding how aspects of biological complexity such as cellular compartmentalization evolved...
  4. ncbi request reprint Evolution of the hemiascomycete yeasts: on life styles and the importance of inbreeding
    Michael Knop
    Cell Biology and Biophysics Unit, EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Bioessays 28:696-708. 2006
    ....
  5. pmc Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast
    Michael Knop
    EMBL, Heidelberg D 69117, Germany
    Mol Biol Cell 16:4543-56. 2005
    ..These results position Mso1p in the interface of the exocyst complex, Sec4p, and the SNARE machinery, and reveal a novel layer of molecular conservation in the exocytosis machinery...
  6. pmc Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane
    Peter Maier
    EMBL, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO J 26:1843-52. 2007
    ....
  7. pmc The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis
    Peter Maier
    EMBL, Cell Biology and Biophysics Unit, Heidelberg, Germany
    EMBO J 27:2363-74. 2008
    ..Rather, repulsive forces generated by asymmetrically distributed and dynamically moving GPI-proteins are suggested as the membrane-bending principle...
  8. ncbi request reprint Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis
    Christian G Riedel
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    J Biol Chem 280:37846-52. 2005
    ..Together, our results suggest that yeast PLD is involved in two distinct but essential steps during the regulated vesicle fusion necessary for the assembly of the membranous encapsulations of the spores...
  9. pmc Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy
    Romain Gibeaux
    European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genes Dev 27:335-49. 2013
    ..Therefore, a twofold symmetrical application of the core principle that drives nuclear migration in higher cells is used in yeast to drive nuclei toward each other before nuclear fusion...
  10. ncbi request reprint Tandem fluorescent protein timers for in vivo analysis of protein dynamics
    Anton Khmelinskii
    Center for Molecular Biology of the University of Heidelberg ZMBH, DKFZ ZMBH Alliance, Heidelberg, Germany
    Nat Biotechnol 30:708-14. 2012
    ..Our experiments reveal the stable nature and asymmetric inheritance of nuclear pore complexes and identify regulators of N-end rule–mediated protein degradation...
  11. ncbi request reprint Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast
    Christof Taxis
    Cell Biology and Biophysics Unit, EMBL, Meyerhofstr 1, Heidelberg 69117, Germany
    Traffic 7:1628-42. 2006
    ..However, actin is essential for spore wall formation. This requires the function of the Arp2/3p complex and involves large carbohydrate-rich compartments, which may be chitosome analogous structures...
  12. pmc Reconstitution of Mdm2-dependent post-translational modifications of p53 in yeast
    Barbara Di Ventura
    European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 3:e1507. 2008
    ....
  13. pmc Evolution of mutational robustness in the yeast genome: a link to essential genes and meiotic recombination hotspots
    Philipp J Keller
    European Molecular Biology Laboratory EMBL, Cell Biology and Biophysics Unit, Heidelberg, Germany
    PLoS Genet 5:e1000533. 2009
    ..Our analysis reveals a new aspect of the evolution of genome architectures that complements insights about molecular constraints, such as the interference of pericentromeric crossovers with chromosome segregation...
  14. ncbi request reprint System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae
    Christof Taxis
    EMBL, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
    Biotechniques 40:73-8. 2006
    ..The set of new vectors considerably enhances the flexibility of genetic manipulations and gene expression in yeast. Most notably, the new vectors allow one to work with natural yeast isolates, which do not contain auxotrophic markers...
  15. pmc When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved
    Romain Gibeaux
    European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    Nucleus 4:182-8. 2013
    ..This review discusses our understanding of yeast nuclear congression and karyogamy and seeks to explain how a detailed, quantitative and systemic understanding has emerged from this knowledge. ..
  16. ncbi request reprint Prospore membrane formation: how budding yeast gets shaped in meiosis
    Alexandra C Moreno-Borchart
    EMBL, Cell Biology and Biophysics Programme, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Microbiol Res 158:83-90. 2003
    ..This mini review discusses our current understanding of how the meiotic plaque and the LEP coat might function during biogenesis of the prospore membrane...
  17. doi request reprint Quantitative fluorescence imaging of protein diffusion and interaction in living cells
    Jérémie Capoulade
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Biotechnol 29:835-9. 2011
    ..We expect that FCS imaging will become a useful method for the precise characterization of cellular reaction-diffusion processes...
  18. pmc Seamless gene tagging by endonuclease-driven homologous recombination
    Anton Khmelinskii
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    PLoS ONE 6:e23794. 2011
    ....
  19. ncbi request reprint Analysis of protein dynamics with tandem fluorescent protein timers
    Anton Khmelinskii
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, Heidelberg, 69120, Germany
    Methods Mol Biol 1174:195-210. 2014
    ..We detail a protocol for quantitative fluorescence microscopy imaging and analysis of intracellular protein dynamics with tFTs in the budding yeast Saccharomyces cerevisiae. ..
  20. ncbi request reprint Yeast cell morphology and sexual reproduction--a short overview and some considerations
    Michael Knop
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    C R Biol 334:599-606. 2011
    ..In this overview article I provide a short summary of the relevant aspects of yeast cell morphology, in particular in relation to one of the most influencing processes, cellular reproduction by mating and meiosis...
  21. ncbi request reprint Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling
    Celine I Maeder
    Cell Biology and Biophysics Unit, EMBL Heidelberg, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 9:1319-26. 2007
    ..Propagation of signalling from the shmoo is, therefore, spatially constrained by a gradient-generating reaction-diffusion mechanism...
  22. pmc Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis
    Oren Gordon
    Department of Genetics, The Hebrew University of Jerusalem, Jerusalem, Israel
    EMBO J 25:3856-68. 2006
    ..Both functions of Nud1p are connected to the ability of Spc72p to bind to the outer plaque and half-bridge (via Kar1p) of the SPB...
  23. ncbi request reprint A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes
    Carsten Janke
    CRBM, CNRS FRE2593, 1919 Route de Mende, F 34293 Montpellier cedex 5, France
    Yeast 21:947-62. 2004
    ..This new toolbox should help to speed up the analysis of gene function in yeast, on the level of single genes, as well as in systematic approaches...
  24. pmc Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions
    Hiromi Maekawa
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    EMBO J 22:438-49. 2003
    ..Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles...