R Klein

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint Bidirectional signals establish boundaries
    R Klein
    European Molecular Biology Laboratory Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Curr Biol 9:R691-4. 1999
  2. ncbi request reprint Eph receptors and ephrins: effectors of morphogenesis
    N Holder
    Department of Anatomy and Developmental Biology, University College, Gower Street, London, WC1 6BT, UK
    Development 126:2033-44. 1999
  3. ncbi request reprint Excitatory Eph receptors and adhesive ephrin ligands
    R Klein
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Curr Opin Cell Biol 13:196-203. 2001
  4. pmc Membrane-bound LERK2 ligand can signal through three different Eph-related receptor tyrosine kinases
    R Brambilla
    European Molecular Biology Laboratory, Differentiation Programme, Heidelberg, Germany
    EMBO J 14:3116-26. 1995
  5. ncbi request reprint The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration
    R H Adams
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 104:57-69. 2001
  6. ncbi request reprint Coupling Met to specific pathways results in distinct developmental outcomes
    F Maina
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 7:1293-306. 2001
  7. ncbi request reprint Kinase-dependent and kinase-independent functions of EphA4 receptors in major axon tract formation in vivo
    K Kullander
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Neuron 29:73-84. 2001
  8. ncbi request reprint Point mutation in trkB causes loss of NT4-dependent neurons without major effects on diverse BDNF responses
    L Minichiello
    European Molecular Biology Laboratory, Heidelberg, Germany
    Neuron 21:335-45. 1998
  9. pmc The N-terminal globular domain of Eph receptors is sufficient for ligand binding and receptor signaling
    J P Labrador
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 16:3889-97. 1997
  10. pmc Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis
    R H Adams
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:295-306. 1999

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Bidirectional signals establish boundaries
    R Klein
    European Molecular Biology Laboratory Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Curr Biol 9:R691-4. 1999
    ..This signalling is mediated by Eph receptors and their ligands, which has been found to restrict cell intermingling in vitro...
  2. ncbi request reprint Eph receptors and ephrins: effectors of morphogenesis
    N Holder
    Department of Anatomy and Developmental Biology, University College, Gower Street, London, WC1 6BT, UK
    Development 126:2033-44. 1999
    ..We address the question of how the specificity of response is achieved and discuss the specificity of ephrin-Eph interactions and the significance of structural domains in Eph receptors...
  3. ncbi request reprint Excitatory Eph receptors and adhesive ephrin ligands
    R Klein
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Curr Opin Cell Biol 13:196-203. 2001
    ..The mode of action of ephrin/Ephs has been expanded from repulsion to adhesion and from cell surface attachment to regulated cleavage...
  4. pmc Membrane-bound LERK2 ligand can signal through three different Eph-related receptor tyrosine kinases
    R Brambilla
    European Molecular Biology Laboratory, Differentiation Programme, Heidelberg, Germany
    EMBO J 14:3116-26. 1995
    ..Quantitative analysis including binding assays indicates that Cek10 is the preferred LERK2 receptor. Preliminary mutagenesis of the LERK2 protein suggests a negative regulatory role for its cytoplasmic domain in LERK2 signaling...
  5. ncbi request reprint The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration
    R H Adams
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 104:57-69. 2001
    ..Our results indicate distinct requirements of functions mediated by the ephrinB carboxyterminus for developmental processes in the vertebrate embryo...
  6. ncbi request reprint Coupling Met to specific pathways results in distinct developmental outcomes
    F Maina
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 7:1293-306. 2001
    ..These data indicate that RTK-mediated activation of specific signaling pathways is required to fulfill cell-specific functions in vivo...
  7. ncbi request reprint Kinase-dependent and kinase-independent functions of EphA4 receptors in major axon tract formation in vivo
    K Kullander
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Neuron 29:73-84. 2001
    ..Unexpectedly, the cytoplasmic sterile-alpha motif (SAM) domain is not required for EphA4 functions. Our findings establish both kinase-dependent and kinase-independent functions of EphA4 in the formation of major axon tracts...
  8. ncbi request reprint Point mutation in trkB causes loss of NT4-dependent neurons without major effects on diverse BDNF responses
    L Minichiello
    European Molecular Biology Laboratory, Heidelberg, Germany
    Neuron 21:335-45. 1998
    ....
  9. pmc The N-terminal globular domain of Eph receptors is sufficient for ligand binding and receptor signaling
    J P Labrador
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 16:3889-97. 1997
    ..Our studies have determined the first structural elements involved in ligand-receptor interaction and will allow more fine-tuned genetic experiments to elucidate the mechanism of action of these important guidance molecules...
  10. pmc Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis
    R H Adams
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:295-306. 1999
    ....
  11. pmc Ephrin-B3 is the midline barrier that prevents corticospinal tract axons from recrossing, allowing for unilateral motor control
    K Kullander
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 15:877-88. 2001
    ....
  12. ncbi request reprint Multiple roles for hepatocyte growth factor in sympathetic neuron development
    F Maina
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Neuron 20:835-46. 1998
    ..Because Met and HGF are expressed in sympathetic ganglia throughout development, it is possible that the multiple effects of HGF/Met signaling on sympathetic neuroblasts and neurons occur in part by an autocrine mechanism...
  13. pmc Met receptor signaling is required for sensory nerve development and HGF promotes axonal growth and survival of sensory neurons
    F Maina
    Cell Regulation Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Genes Dev 11:3341-50. 1997
    ..In contrast, the actions of HGF on sensory neurons are mediated by Met effectors distinct from Grb2. Our findings demonstrate a requirement for Met signaling in neurons during development...
  14. ncbi request reprint Signaling by Eph receptors and their ephrin ligands
    K Brückner
    European Molecular Biology Laboratory, Heidelberg, Germany
    Curr Opin Neurobiol 8:375-82. 1998
    ....
  15. ncbi request reprint EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains
    K Brückner
    Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Neuron 22:511-24. 1999
    ..Our findings suggest that GRIP proteins provide a scaffold for the assembly of a multiprotein signaling complex downstream of ephrinB ligands...
  16. ncbi request reprint Essential role for TrkB receptors in hippocampus-mediated learning
    L Minichiello
    European Molecular Biology Laboratory, Heidelberg, Germany
    Neuron 24:401-14. 1999
    ..Interestingly, heterozygotes show a partial but substantial reduction of LTP but appear behaviorally normal. Thus, CA1 LTP may need to be reduced below a certain threshold before behavioral defects become apparent...
  17. ncbi request reprint Essential role of p38alpha MAP kinase in placental but not embryonic cardiovascular development
    R H Adams
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell 6:109-16. 2000
    ..Our results indicate that p38alpha is required for placental organogenesis but is not essential for other aspects of mammalian embryonic development...
  18. ncbi request reprint The Eph receptor family: axonal guidance by contact repulsion
    D Orioli
    Cell Regulation Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Trends Genet 13:354-9. 1997
    ..Here, we summarize the known biological functions of Eph receptors and ligands focusing on the developing nervous system, and we review the data on possible mechanisms of action...
  19. pmc Sek4 and Nuk receptors cooperate in guidance of commissural axons and in palate formation
    D Orioli
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 15:6035-49. 1996
    ..These results demonstrate essential and cooperative functions for Sek4 and Nuk in establishing axon pathways in the developing brain, and during the development of facial structures...
  20. ncbi request reprint Similarities and differences in the way transmembrane-type ligands interact with the Elk subclass of Eph receptors
    R Brambilla
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell Neurosci 8:199-209. 1996
    ..Knowledge regarding functional ligand-receptor interactions as presented in this study will be important for the design and interpretation of in vivo experiments, e.g., loss-of-function studies in transgenic mice...
  21. pmc The collagen receptor DDR2 regulates proliferation and its elimination leads to dwarfism
    J P Labrador
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO Rep 2:446-52. 2001
    ..Together our results suggest that DDR2 acts as an extracellular matrix sensor to modulate cell proliferation...
  22. ncbi request reprint Hepatocyte growth factor, a versatile signal for developing neurons
    F Maina
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Neurosci 2:213-7. 1999
    ..The broad spectrum of HGF activities and its observed synergy with other neurotrophic factors suggest that the major role of HGF is to potentiate the response of developing neurons to specific signals...
  23. ncbi request reprint A role for the Ras signalling pathway in synaptic transmission and long-term memory
    R Brambilla
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nature 390:281-6. 1997
    ..These results implicate Ras-GRF signalling via the Ras/MAP kinase pathway in synaptic events leading to formation of long-term memories...
  24. ncbi request reprint TrkB and TrkC neurotrophin receptors cooperate in promoting survival of hippocampal and cerebellar granule neurons
    L Minichiello
    European Molecular Biology Laboratory, Heidelberg, Germany
    Genes Dev 10:2849-58. 1996
    ..Moreover, they suggest that central, in contrast to peripheral, neurons are capable of using more than one neurotrophin/Trk receptor signaling pathway to stay alive...
  25. ncbi request reprint Uncoupling of Grb2 from the Met receptor in vivo reveals complex roles in muscle development
    F Maina
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Cell 87:531-42. 1996
    ..These data show that the requirements for Met signaling vary depending on the tissue and reveal a novel role for HGF/ Met in late myogenesis...
  26. ncbi request reprint Nuk controls pathfinding of commissural axons in the mammalian central nervous system
    M Henkemeyer
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cell 86:35-46. 1996
    ..These results indicate that Nuk, a receptor that binds transmembrane ligands, plays a critical and unique role in the pathfinding of specific axons in the mammalian central nervous system...
  27. ncbi request reprint Kinase-independent requirement of EphB2 receptors in hippocampal synaptic plasticity
    I C Grunwald
    Department of Molecular Neurobiology, Max Planck Institute of Neurobiology, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Neuron 32:1027-40. 2001
    ..Interestingly, targeted expression of a carboxy-terminally truncated form of EphB2 rescued the EphB2 null phenotype, indicating that EphB2 kinase signaling is not required for these EphB2-mediated functions...
  28. ncbi request reprint TrkB and neurotrophin-4 are important for development and maintenance of sympathetic preganglionic neurons innervating the adrenal medulla
    A Schober
    Department of Anatomy and Cell Biology III, University of Heidelberg, D 69120 Heidelberg, Germany
    J Neurosci 18:7272-84. 1998
    ..These data are consistent with the notion that NT-4 from chromaffin cells operates through TrkB receptors to regulate development and maintenance of the preganglionic innervation of the adrenal medulla...
  29. ncbi request reprint Developing inner ear sensory neurons require TrkB and TrkC receptors for innervation of their peripheral targets
    T Schimmang
    Departamento Bioquimica, Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid, CSIC, Spain
    Development 121:3381-91. 1995
    ..TrkB and TrkC receptors are therefore required for the survival of specific neuronal populations and the maintenance of target innervation in the peripheral sensory system of the inner ear...
  30. ncbi request reprint Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex
    D Graus-Porta
    Friedrich Miescher Institute, Maulbeerstr 66, 4058, Basel, Switzerland
    Neuron 31:367-79. 2001
    ..The phenotype of the beta1-deficient mice resembles pathological changes observed in human cortical dysplasias, suggesting that defective integrin-mediated signal transduction contributes to the development of some of these diseases...
  31. ncbi request reprint Severe sensory and sympathetic neuropathies in mice carrying a disrupted Trk/NGF receptor gene
    R J Smeyne
    Department of Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543 4000
    Nature 368:246-9. 1994
    ..These findings demonstrate that Trk is the primary mediator of the trophic actions of NGF in vivo and that this signalling pathway plays a crucial role in the development of both the peripheral and the central nervous systems...
  32. pmc ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites
    A D Bergemann
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 15:4921-9. 1995
    ..Comparable patterns have been described for Eph family receptors, including Sek-4 and Nuk/Sek-3, suggesting roles for ELF-2 in patterning these regions of the embryo...
  33. ncbi request reprint Similarities and differences in the way neurotrophins interact with the Trk receptors in neuronal and nonneuronal cells
    N Y Ip
    Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591
    Neuron 10:137-49. 1993
    ..However, we find that a neuronal environment does restrict the Trks in their ability to respond to their "nonpreferred" neurotrophin ligands...
  34. ncbi request reprint Neutral endopeptidase terminates substance P-induced inflammation in allergic contact dermatitis
    T E Scholzen
    Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, Department of Dermatology, University of Munster, Munster, Germany
    J Immunol 166:1285-91. 2001
    ..Thus, these results indicate that NEP and cutaneous neuropeptides have a significant role in the pathogenesis of ACD...
  35. ncbi request reprint Role of brain insulin receptor in control of body weight and reproduction
    J C Bruning
    Klinik II und Poliklinik für Innere Medizin and Center of Molecular Medicine ZMMK der Universität zu Köln, Joseph Stelzmann Strasse 9, 50931 Cologne, Germany
    Science 289:2122-5. 2000
    ..Thus, IR signaling in the CNS plays an important role in regulation of energy disposal, fuel metabolism, and reproduction...
  36. ncbi request reprint Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements
    R Klein
    Department of Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543
    Nature 368:249-51. 1994
    ..These mice display abnormal movements and postures, indicating that NT3/TrkC-dependent sensor; neurons may play a primary role in proprioception, the sense of position and movement of the limbs...
  37. ncbi request reprint Reduced acetylcholinesterase (AChE) activity in adrenal medulla and loss of sympathetic preganglionic neurons in TrkA-deficient, but not TrkB-deficient, mice
    A Schober
    Department of Anatomy and Cell Biology III, University of Heidelberg, Germany
    J Neurosci 17:891-903. 1997
    ..Our results suggest that signaling via TrkA, but not TrkB, may be involved in the postnatal regulation of AChE activity in the adrenal medulla and its preganglionic nerves...
  38. ncbi request reprint Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube
    A D Bergemann
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Oncogene 16:471-80. 1998
    ..The isolation of this ligand may help to elucidate the molecular basis of patterning activities at the neural tube midline...
  39. ncbi request reprint Timing of neuronal death in trkA, trkB and trkC mutant embryos reveals developmental changes in sensory neuron dependence on Trk signalling
    L G Pinon
    School of Biological and Medical Sciences, University of St Andrews, Fife, Scotland, UK
    Development 122:3255-61. 1996
    ..These results demonstrate that TrkB signalling is required for the in vivo survival of many trigeminal neurons during the early stages of target field innervation before they become NGF-dependent...
  40. ncbi request reprint Aberrant neural and cardiac development in mice lacking the ErbB4 neuregulin receptor
    M Gassmann
    Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 378:390-4. 1995
    ....
  41. ncbi request reprint Tyro-3 family receptors are essential regulators of mammalian spermatogenesis
    Q Lu
    Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 398:723-8. 1999
    ..Additional observations indicate that these receptors may also be essential for the tropic maintenance of diverse cell types in the mature nervous, immune and reproductive systems...
  42. ncbi request reprint Differential effects of combined trk receptor mutations on dorsal root ganglion and inner ear sensory neurons
    L Minichiello
    Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Development 121:4067-75. 1995
    ..This effect was not observed in DRGs, where neurons depending on different Trk receptors generally innervate different targets...
  43. ncbi request reprint Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death
    R Klein
    Department of Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543 4000
    Cell 75:113-22. 1993
    ..These findings illustrate the role of the gp145trkB protein-tyrosine kinase receptor in the ontogeny of the mammalian nervous system...
  44. ncbi request reprint The branchial arches and HGF are growth-promoting and chemoattractant for cranial motor axons
    A Caton
    Centre for Developmental Neurobiology, King s College, Guy s Campus, London SE1 9RT, UK
    Development 127:1751-66. 2000
    ..This influence is likely to be mediated partly by Hepatocyte Growth Factor, although a component of branchial arch-mediated growth promotion and chemoattraction was not blocked by anti-HGF antibodies...
  45. ncbi request reprint Survival of inner ear sensory neurons in trk mutant mice
    T Schimmang
    Instituto de Biologia y Genetica Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Cientificas, Facultad de Medicina, Spain
    Mech Dev 64:77-85. 1997
    ....