Genomes and Genes
Affiliation: European Molecular Biology Laboratory
- A journey from reductionist to systemic cell biology aboard the schooner TaraEric Karsenti
European Molecular Biology Laboratory, D69117 Heidelberg, Germany
Mol Biol Cell 23:2403-6. 2012....
- A holistic approach to marine eco-systems biologyEric Karsenti
European Molecular Biology Laboratory EMBL, Heidelberg, Germany
PLoS Biol 9:e1001177. 2011..The Tara Oceans consortium organizes expeditions to help fill this gap at the global level...
- The mitotic spindle and actin tailsEric Karsenti
Cell Biology and Biophysics Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Biol Cell 96:237-40. 2004..We wonder in this respect whether lower eukaryotes such as yeasts are different from higher eukaryotes such as vertebrates...
- Modelling microtubule patternsEric Karsenti
European Molecular Systems Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nat Cell Biol 8:1204-11. 2006..We illustrate how this led to the beginning of an understanding of dynamic cellular patterns that have precise functions...
- Self-organization in cell biology: a brief historyEric Karsenti
Eric Karsenti is at the Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nat Rev Mol Cell Biol 9:255-62. 2008..Self-organization concepts that were first developed in chemistry and physics and then applied to various morphogenetic problems in biology over the past century are now beginning to be applied to the organization of the living cell...
- Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rateHideki Yokoyama
European Molecular Biology Laboratory, Heidelberg 69117, Germany
J Cell Biol 180:867-75. 2008..Our results indicate that Cdk11 is responsible for RanGTP-dependent MT stabilization around chromosomes and that this local stabilization is essential for normal rates of spindle assembly and spindle function...
- Hepatoma up-regulated protein is required for chromatin-induced microtubule assembly independently of TPX2Claudia M Casanova
European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Mol Biol Cell 19:4900-8. 2008..Our data indicate that HURP and TPX2 have nonredundant functions essential for chromatin-induced microtubule assembly...
- Regulation of microtubule dynamics by reaction cascades around chromosomesChaitanya A Athale
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, Heidelberg, Germany
Science 322:1243-7. 2008..We propose a reaction-diffusion model that combines the chromosome generated Ran-guanosine triphosphate-Importin reaction network to a secondary phosphorylation network as a potential mechanism for the generation of such gradients...
- ISWI is a RanGTP-dependent MAP required for chromosome segregationHideki Yokoyama
European Molecular Biology Laboratory, Heidelberg 69117, Germany
J Cell Biol 187:813-29. 2009..Our results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle MTs in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation...
- Spatial coordination of spindle assembly by chromosome-mediated signaling gradientsMaiwen Caudron
Cell Biology and Biophysics Department, European Molecular Biology Laboratory EMBL, EMBL, 69117 Heidelberg, Germany
Science 309:1373-6. 2005..This produces activity gradients that determine the spatial distribution of microtubule nucleation and stabilization around chromosomes and that are essential for the self-organization of microtubules into a bipolar spindle...
- Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extractsStephane Brunet
Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg 69 117, Germany
Mol Biol Cell 15:5318-28. 2004..These and previous results suggest that under physiological conditions, TPX2 is essential for microtubule nucleation around chromatin and functions in a network of other molecules, some of which also are regulated by RanGTP...
- XMAP215-EB1 interaction is required for proper spindle assembly and chromosome segregation in Xenopus egg extractIva Kronja
Department of Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, Germany
Mol Biol Cell 20:2684-96. 2009..Therefore, the XMAP215-EB1 interaction is required for proper spindle organization and chromosome segregation in Xenopus egg extracts...
- Gradients in the self-organization of the mitotic spindlePhilippe Bastiaens
Cell Biology and Biophysics Program, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Trends Cell Biol 16:125-34. 2006..We argue that such reaction diffusion processes are involved in the self-organization of supramolecular structures in the cell...
- Stathmin-tubulin interaction gradients in motile and mitotic cellsPhilipp Niethammer
European Molecular Biology Laboratory, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Science 303:1862-6. 2004..These interaction patterns are likely to reflect phosphorylation of stathmin in these areas...
- Chromatin shapes the mitotic spindleAna Dinarina
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg D 69117, Germany
Cell 138:502-13. 2009..Our results show that chromatin defines spindle shape and orientation. For a video summary of this article, see the PaperFlick file available with the online Supplemental Data...
- Discrete states of a protein interaction network govern interphase and mitotic microtubule dynamicsPhilipp Niethammer
Cell Biology and Biophysics Department, European Molecular Biology Laboratory, Heidelberg, Germany
PLoS Biol 5:e29. 2007..This provides the beginning of a biochemical description of the notion of "cytoplasmic states" regarding the microtubule system...
- TPX or not TPX?Eric Karsenti
Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
Mol Cell 19:431-2. 2005..elegans. The diverse roles of these two proteins in spindle assembly leave us to beckon the true C. elegans TPX2 ortholog to center stage...
- Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cellsOliver J Gruss
EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
Nat Cell Biol 4:871-9. 2002..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells...
- Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger proteinVéronique Pizon
European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
J Cell Sci 115:4469-82. 2002..MURF2 can therefore act as a transient adaptor between microtubules, titin and nascent myosin filaments, as well as being involved in signalling from the sarcomere to the nucleus...
- Self-organisation and forces in the microtubule cytoskeletonFrancois Nedelec
EMBL, Cell Biology and Biophysics Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Curr Opin Cell Biol 15:118-24. 2003..Together, these observations suggest that the spindle can be experimentally deconstructed, and that its defining characteristics can be studied in a simplified context, in the absence of the full division machinery...
- Long-range communication between chromatin and microtubules in Xenopus egg extractsRafael E Carazo-Salas
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Curr Biol 13:1728-33. 2003..To our knowledge, this is the first direct evidence of a long-range guidance effect at the sub-cellular level...
- Importin alpha-regulated nucleation of microtubules by TPX2Christoph A Schatz
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
EMBO J 22:2060-70. 2003..The data suggest that the critical early function in spindle formation regulated by importin alpha is TPX2-mediated microtubule nucleation...
- XMAP215 is required for the microtubule-nucleating activity of centrosomesAndrei V Popov
Cell Biology Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117, Heidelberg, Germany
Curr Biol 12:1326-30. 2002....
- Stu2p and XMAP215: turncoat microtubule-associated proteins?Andrei V Popov
INSERM Unité 366, DRDC CS, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
Trends Cell Biol 13:547-50. 2003..Two related MAPs, XMAP215 and Stu2p, are known to stabilize MTs. However, recent studies report that these proteins have a MT-destabilizing function as well. Here we discuss the implications of these reports...
- Cytoskeleton: spindle sagaEric Karsenti
Nature 432:563-4. 2004
- Eg5 causes elongation of meiotic spindles when flux-associated microtubule depolymerization is blockedMimi Shirasu-Hiza
Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
Curr Biol 14:1941-5. 2004..These results suggest that Eg5 is responsible for flux-associated MT translocation and that dynein and Xklp2 regulate flux-associated microtubule depolymerization at spindle poles...
- Homology-based functional proteomics by mass spectrometry: application to the Xenopus microtubule-associated proteomeAdam J Liska
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Proteomics 4:2707-21. 2004..These findings were made possible due to the application of sequence-similarity methods, which extended mass spectrometric protein identification capabilities by 2-fold compared to conventional methods...
- Microtubule-dependent transport and organization of sarcomeric myosin during skeletal muscle differentiationVéronique Pizon
Institut Jacques Monod, Paris, France
EMBO J 24:3781-92. 2005..Collectively, our results strongly suggest that MTs become organized into a scaffold that provides directional cues for the positioning and organization of myosin filaments during sarcomere formation...
- Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversionStephane Brunet
Biologie du Développement, CNRS UMR 7622 bat C 5ème, 9, quai St Bernard, 75252 Paris Cedex 05, France
Traffic 7:1283-9. 2006..It has the potential to be extended to study interactions of proteins or multi-protein complexes with different biopolymers like actin microfilaments or organelle membranes...