Matthias W Hentze

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint Y14 and hUpf3b form an NMD-activating complex
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    Mol Cell 11:939-49. 2003
  2. ncbi request reprint Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
  3. pmc Integration of splicing, transport and translation to achieve mRNA quality control by the nonsense-mediated decay pathway
    Thomas Schell
    EMBL Heidelberg, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 3:REVIEWS1006. 2002
  4. doi request reprint Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression
    Agnieszka Zdanowicz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany
    Mol Cell 35:881-8. 2009
  5. doi request reprint Translational control via protein-regulated upstream open reading frames
    Jan Medenbach
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 145:902-13. 2011
  6. pmc A co-repressor assembly nucleated by Sex-lethal in the 3'UTR mediates translational control of Drosophila msl-2 mRNA
    Marica Grskovic
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO J 22:5571-81. 2003
  7. pmc Sex-lethal imparts a sex-specific function to UNR by recruiting it to the msl-2 mRNA 3' UTR: translational repression for dosage compensation
    Kent Duncan
    Gene Expression Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Genes Dev 20:368-79. 2006
  8. doi request reprint Iron regulatory proteins secure mitochondrial iron sufficiency and function
    Bruno Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell Metab 12:194-201. 2010
  9. pmc Mechanism of translational regulation by miR-2 from sites in the 5' untranslated region or the open reading frame
    Francesca Moretti
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    RNA 16:2493-502. 2010
  10. ncbi request reprint Iron regulation and the cell cycle: identification of an iron-responsive element in the 3'-untranslated region of human cell division cycle 14A mRNA by a refined microarray-based screening strategy
    Mayka Sanchez
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:22865-74. 2006

Collaborators

Detail Information

Publications50

  1. ncbi request reprint Y14 and hUpf3b form an NMD-activating complex
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    Mol Cell 11:939-49. 2003
    ..These results uncover a direct role of Y14 in NMD and suggest an unexpected hierarchy in the assembly of NMD complexes...
  2. ncbi request reprint Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
    ..These results are integrated into a nonlinear model for mammalian NMD involving alternative routes of entry that converge at a common requirement of UPF1...
  3. pmc Integration of splicing, transport and translation to achieve mRNA quality control by the nonsense-mediated decay pathway
    Thomas Schell
    EMBL Heidelberg, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Genome Biol 3:REVIEWS1006. 2002
    ..New findings suggest that these exon-exon junction complexes and the complexes that bind mRNA caps are key effectors of the fate of spliced mRNAs and may regulate whether mRNAs containing premature stop codons are degraded...
  4. doi request reprint Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression
    Agnieszka Zdanowicz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany
    Mol Cell 35:881-8. 2009
    ....
  5. doi request reprint Translational control via protein-regulated upstream open reading frames
    Jan Medenbach
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 145:902-13. 2011
    ..We propose that protein-regulated uORFs constitute a systematic principle for the regulation of protein synthesis...
  6. pmc A co-repressor assembly nucleated by Sex-lethal in the 3'UTR mediates translational control of Drosophila msl-2 mRNA
    Marica Grskovic
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO J 22:5571-81. 2003
    ..Our data support a model where dSXL binding to the 3'UTR of msl-2 mRNA activates the translational repressor domain, thereby enabling it to recruit co-repressors in a specific fashion...
  7. pmc Sex-lethal imparts a sex-specific function to UNR by recruiting it to the msl-2 mRNA 3' UTR: translational repression for dosage compensation
    Kent Duncan
    Gene Expression Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Genes Dev 20:368-79. 2006
    ..Our results reveal a novel functional role for UNR as a translational repressor and indicate that UNR is a key component of a "fail-safe" dosage compensation regulatory system that prevents toxic MSL-2 synthesis in female cells...
  8. doi request reprint Iron regulatory proteins secure mitochondrial iron sufficiency and function
    Bruno Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell Metab 12:194-201. 2010
    ..These results uncover a major role of the IRPs in cell biology: to ensure adequate iron supply to the mitochondrion for proper function of this critical organelle...
  9. pmc Mechanism of translational regulation by miR-2 from sites in the 5' untranslated region or the open reading frame
    Francesca Moretti
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    RNA 16:2493-502. 2010
    ..Using single or dual binding sites, it also reveals a biological rationale for the high prevalence of miR regulatory sites in the 3'UTR...
  10. ncbi request reprint Iron regulation and the cell cycle: identification of an iron-responsive element in the 3'-untranslated region of human cell division cycle 14A mRNA by a refined microarray-based screening strategy
    Mayka Sanchez
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:22865-74. 2006
    ..This work describes a new experimental strategy to explore the IRE/IRP regulatory network and uncovers a previously unrecognized regulatory link between iron metabolism and the cell cycle...
  11. pmc Translation initiation by the c-myc mRNA internal ribosome entry sequence and the poly(A) tail
    Christian Thoma
    European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    RNA 14:1579-89. 2008
    ..The mechanistic distinctions from cap-dependent translation may allow specific translational control of the c-myc mRNA and possibly other cellular mRNAs that initiate translation via IRESs...
  12. ncbi request reprint Bruno acts as a dual repressor of oskar translation, promoting mRNA oligomerization and formation of silencing particles
    Marina Chekulaeva
    Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 124:521-33. 2006
    ..Bruno-dependent mRNA oligomerization into silencing particles emerges as a mode of translational control that may be particularly suited to coupling with mRNA transport...
  13. ncbi request reprint Enhancement of IRES-mediated translation of the c-myc and BiP mRNAs by the poly(A) tail is independent of intact eIF4G and PABP
    Christian Thoma
    EMBL Heidelberg, Gene Expression Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Mol Cell 15:925-35. 2004
    ..The polyadenylated mRNA translation from cellular IRESs is also profoundly sensitive to eIF4A activity in vitro. These mechanistic and molecular distinctions implicate the potential for a new layer of translational control mechanisms...
  14. pmc Eukaryotic translation initiation factor 4GI and p97 promote cellular internal ribosome entry sequence-driven translation
    Patrick Hundsdoerfer
    Gene Expression Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Proc Natl Acad Sci U S A 102:13421-6. 2005
    ....
  15. pmc SIREs: searching for iron-responsive elements
    Monica Campillos
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nucleic Acids Res 38:W360-7. 2010
    ..The SIREs (Search for iron-responsive elements) web server is freely available on the web at http://ccbg.imppc.org/sires/index.html...
  16. doi request reprint In vivo role(s) of the iron regulatory proteins (IRP) 1 and 2 in aseptic local inflammation
    Lydie Viatte
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    J Mol Med (Berl) 87:913-21. 2009
    ..Our data indicate that the response of Irp1 (-/-) and Irp2 (-/-) mice to acute local inflammation is largely preserved...
  17. ncbi request reprint Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation
    Rolf Thermann
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 447:875-8. 2007
    ..These results directly show the inhibition of m7GpppG cap-mediated translation initiation as the mechanism of miR2 function, and uncover pseudo-polysomal messenger ribonucleoprotein assemblies that may help to explain earlier findings...
  18. doi request reprint Iron regulatory proteins are essential for intestinal function and control key iron absorption molecules in the duodenum
    Bruno Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
    Cell Metab 7:79-85. 2008
    ..IRPs are thus essential for intestinal function and organismal survival and coordinate the synthesis of key iron metabolism proteins in the duodenum...
  19. doi request reprint Iron regulatory proteins control a mucosal block to intestinal iron absorption
    Bruno Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany
    Cell Rep 3:844-57. 2013
    ..IRPs thus secure sufficient iron transport across absorptive enterocytes by restricting the ferritin "mucosal block" and define a basal set point for iron absorption upon which IRP-independent systemic regulatory inputs are overlaid...
  20. ncbi request reprint Drosophila sex-lethal inhibits the stable association of the 40S ribosomal subunit with msl-2 mRNA
    Fátima Gebauer
    European Molecular Biology Laboratory, Gene Expression Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Mol Cell 11:1397-404. 2003
    ..These results elucidate a critical regulatory step for dosage compensation in Drosophila melanogaster...
  21. ncbi request reprint Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock
    Thomas Preiss
    EMBL Heidelberg, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Struct Biol 10:1039-47. 2003
    ..Thus, signal-induced changes in the transcriptome are amplified at the translational level. These results unveil a higher level of coordinated gene regulation that we refer to as 'potentiation.'..
  22. ncbi request reprint A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain
    Susanne Till
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Struct Mol Biol 14:897-903. 2007
    ..Proteins containing the conserved Ago hook may thus be important regulatory components of effector complexes in RNA interference...
  23. doi request reprint Two to tango: regulation of Mammalian iron metabolism
    Matthias W Hentze
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 142:24-38. 2010
    ..We also highlight some of the current questions in mammalian iron metabolism and discuss therapeutic opportunities arising from a better understanding of the underlying biological principles...
  24. doi request reprint The REM phase of gene regulation
    Matthias W Hentze
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Trends Biochem Sci 35:423-6. 2010
    ....
  25. ncbi request reprint A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells
    Christian Thoma
    Gene Expression Programme, EMBL, Heidelberg, Germany
    Methods Mol Biol 257:171-80. 2004
    ..We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines...
  26. pmc Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo
    Bruno Galy
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    RNA 10:1019-25. 2004
    ..We discuss the implications for the understanding of RNA processing and for targeting strategies for functional genomics in the mouse...
  27. doi request reprint RNA-binding proteins in Mendelian disease
    Alfredo Castello
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Trends Genet 29:318-27. 2013
    ..We also expect that mRNA interactome capture approaches will aid further exploration of RNA systems biology in varied physiological and pathophysiological settings...
  28. pmc Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells
    Thomas Schell
    European Molecular Biology Laboratory Heidelberg, Gene Expression Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Biochem J 373:775-83. 2003
    ..Components of the exon-exon junction complex or the translational eukaryotic release factor (eRF) 3 were not identified in complexes associated with Hupf1-2tag. We discuss these findings in the context of current models of NMD...
  29. pmc The interaction of the cap-binding complex (CBC) with eIF4G is dispensable for translation in yeast
    Julie Baron-Benhamou
    Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    RNA 9:654-62. 2003
    ....
  30. pmc Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: implications for regulation and cellular function
    Nadia Hubert
    European Molecular Biology Laboratory, Gene Expression Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Proc Natl Acad Sci U S A 99:12345-50. 2002
    ..These results uncover an unexpected complexity of DMT1 expression and regulation, with implications for understanding the physiology, cell biology, and pathophysiology of mammalian iron metabolism...
  31. ncbi request reprint Abnormally spliced beta-globin mRNAs: a single point mutation generates transcripts sensitive and insensitive to nonsense-mediated mRNA decay
    Sven Danckwardt
    Department of General Pediatrics, Charite, Humboldt University, Berlin, Germany
    Blood 99:1811-6. 2002
    ..It can therefore serve as a useful tool for defining the mechanisms that permit specific transcripts to circumvent the NMD pathway...
  32. pmc Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation
    Joachim B Kunz
    Department for Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    RNA 12:1015-22. 2006
    ..Stimulation of translation is independent of this interaction and is determined by other regions of the hUpf3 protein, indicating the presence of different downstream pathways of hUpf3 proteins either in NMD or in translation...
  33. ncbi request reprint Molecular mechanisms of translational control
    Fátima Gebauer
    Centre de Regulacio Genomica, Passeig Maritim 37 49, 08003 Barcelona, Spain
    Nat Rev Mol Cell Biol 5:827-35. 2004
    ..Instead of providing a comprehensive account of the examples that are known at present, we discuss instructive cases that serve as paradigms for different modes of translational control...
  34. ncbi request reprint Generation of conditional alleles of the murine Iron Regulatory Protein (IRP)-1 and -2 genes
    Bruno Galy
    European Molecular Biology Laboratory, Heidelberg, Germany
    Genesis 43:181-8. 2005
    ..If not removed, the gene trap construct causes partially penetrant embryonic lethality unrelated to IRP deficiency when inserted within the Irp1 but not the Irp2 locus. We discuss the implications for functional genomics in the mouse...
  35. ncbi request reprint A dual inhibitory mechanism restricts msl-2 mRNA translation for dosage compensation in Drosophila
    Karsten Beckmann
    Gene Expression Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 122:529-40. 2005
    ..This failsafe mechanism thus forms the molecular basis of a critical step in dosage compensation. The results also elucidate a two step principle of translational control via multiple regulatory sites within an mRNA...
  36. ncbi request reprint Nonsense-mediated decay approaches the clinic
    Jill A Holbrook
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, D 69120 Heidelberg, Germany
    Nat Genet 36:801-8. 2004
    ..Potential 'NMD therapeutics' will therefore need to strike a balance between the general physiological benefits of NMD and its detrimental effects in cases of specific genetic mutations...
  37. pmc c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs
    Antje Ostareck-Lederer
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell Biol 22:4535-43. 2002
    ..Our results establish a novel role of c-Src kinase in translational gene regulation and reveal a mechanism by which silenced mRNAs can be translationally activated...
  38. ncbi request reprint The human intronless melanocortin 4-receptor gene is NMD insensitive
    Katja S Brocke
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 150, D 69120 Heidelberg, Germany
    Hum Mol Genet 11:331-5. 2002
    ..Thus, the naturally intronless MC4-R gene and probably many other intronless genes fail to be monitored by the NMD pathway...
  39. ncbi request reprint Relationships and distinctions in iron-regulatory networks responding to interrelated signals
    Martina Muckenthaler
    European Molecular Biology Laboratory, Heidelberg, Germany
    Blood 101:3690-8. 2003
    ..These findings also demonstrate the broad utility of gene-expression profiling with the IronChip to study iron metabolism and related human diseases...
  40. pmc Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay
    Jill A Holbrook
    European Molecular Biology Laboratory, University Hospital Heidelberg, Molecular Medicine Partnership Unit, University of Heidelberg, Im Neuenheimer Feld 150, Heidelberg 69120, Germany
    EMBO Rep 7:722-6. 2006
    ..These data generalize the previous model and suggest that translation per se, irrespective of how it is initiated, can mediate NMD...
  41. pmc Complex translational regulation of BACE1 involves upstream AUGs and stimulatory elements within the 5' untranslated region
    Marija Mihailovich
    San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy
    Nucleic Acids Res 35:2975-85. 2007
    ..Furthermore, we have evidence that a sequence within the region 222-323 of the BACE1 5' UTR has a stimulatory effect on translation that might depend on the presence of trans-acting factors...
  42. ncbi request reprint Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7
    Tetsuo Ohnishi
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Mol Cell 12:1187-200. 2003
    ..We propose that sequential phosphorylation and dephosphorylation of hUPF1 by hSMG-1 and PP2A, respectively, contribute to the remodeling of the mRNA surveillance complex...
  43. doi request reprint A bone morphogenetic protein (BMP)-responsive element in the hepcidin promoter controls HFE2-mediated hepatic hepcidin expression and its response to IL-6 in cultured cells
    Maria Vittoria Verga Falzacappa
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    J Mol Med (Berl) 86:531-40. 2008
    ..These data uncover a missing link in the HFE2-mediated control of hepcidin expression and suggest that the BMP-RE controls hepcidin promoter activity mediated by HFE2 and inflammatory stimuli...
  44. ncbi request reprint Using the lambdaN peptide to tether proteins to RNAs
    Julie Baron-Benhamou
    Rockefeller University, New York, NY, USA
    Methods Mol Biol 257:135-54. 2004
    ..The chapter illustrates the use of this system to create dedicated mRNA-specific factors involved in processes, such as mRNA translation and nonsense-mediated mRNA decay...
  45. pmc Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    EMBO J 26:2658-69. 2007
    ..These data uncover a novel mechanism that functionally links the splicing and 3' end formation machineries of multiple cellular mRNAs in an USE-dependent manner...
  46. ncbi request reprint Studying translational control in Drosophila cell-free systems
    Fátima Gebauer
    Centre de Regulació Genòmica CRG UPF, Barcelona, Spain
    Methods Enzymol 429:23-33. 2007
    ..In this chapter we provide general guidelines and detailed protocols to obtain and use translation extracts derived from Drosophila embryos and ovaries...
  47. pmc Nonsense-mediated mRNA decay: from vacuum cleaner to Swiss army knife
    Gabriele Neu-Yilik
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany
    Genome Biol 5:218. 2004
    ..Two new approaches have identified physiological NMD substrates, and suggest that NMD functions as a multipurpose tool in the modulation of gene expression...
  48. ncbi request reprint Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays
    Mayka Sanchez
    Molecular Medicine Partnership Unit MMPU, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Nat Protoc 2:2033-42. 2007
    ..The mRNA composition of the supernatant and/or immunoprecipitated fraction is analyzed using dual-color microarrays in comparison with control reaction. From start to finish, the protocol takes approximately 6 d...
  49. pmc The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway
    Marcelo H Viegas
    Department of Pediatric Oncology, Hematology and Immunology, Children s Hospital, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany
    Nucleic Acids Res 35:4542-51. 2007
    ..We conclude that cellular concentrations of RNPS1 can modify NMD efficiency and propose that cell type specific co-factor availability represents a novel principle that controls NMD...
  50. pmc Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
    ..The EJC, with UPF2 or UPF3b as a cofactor, interferes with physiological termination through UPF1. This model integrates previously competing models of NMD and suggests a mechanistic basis for alternative NMD pathways...