Gareth Griffiths

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint Cell evolution and the problem of membrane topology
    Gareth Griffiths
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:1018-24. 2007
  2. pmc Phagosome proteomes open the way to a better understanding of phagosome function
    Gareth Griffiths
    Cell Biology Program, EMBL, Heidelberg, Germany
    Genome Biol 8:207. 2007
  3. doi request reprint Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen
    Mark P Kuehnel
    EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Cell Sci 122:499-504. 2009
  4. doi request reprint Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion
    Sabrina Marion
    Department of Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, Germany
    Traffic 12:421-37. 2011
  5. doi request reprint Role of lipids in killing mycobacteria by macrophages: evidence for NF-kappaB-dependent and -independent killing induced by different lipids
    Maximiliano Gabriel Gutierrez
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany
    Cell Microbiol 11:406-20. 2009
  6. doi request reprint Golgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin
    Anna Wähe
    European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany
    J Cell Sci 123:2502-11. 2010
  7. ncbi request reprint cAMP synthesis and degradation by phagosomes regulate actin assembly and fusion events: consequences for mycobacteria
    Stefanos A Kalamidas
    EMBL, Postfach 102209, 69117 Heidelberg, Germany
    J Cell Sci 119:3686-94. 2006
  8. doi request reprint Sphingosine-1-phosphate receptors stimulate macrophage plasma-membrane actin assembly via ADP release, ATP synthesis and P2X7R activation
    Mark P Kuehnel
    EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Cell Sci 122:505-12. 2009
  9. pmc Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
    Paras K Anand
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 5:e10136. 2010
  10. ncbi request reprint Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies
    Holger Kress
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Phys Rev E Stat Nonlin Soft Matter Phys 71:061927. 2005

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Cell evolution and the problem of membrane topology
    Gareth Griffiths
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:1018-24. 2007
    ..An invagination of the membrane, aided by an early cytoskeletal system, may have formed the first cells--initially within primordial vesicles...
  2. pmc Phagosome proteomes open the way to a better understanding of phagosome function
    Gareth Griffiths
    Cell Biology Program, EMBL, Heidelberg, Germany
    Genome Biol 8:207. 2007
    ..Studies on the protein and lipid composition of model phagosomes containing latex beads are the first step in a systems-biology approach to understanding how these organelles function...
  3. doi request reprint Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen
    Mark P Kuehnel
    EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Cell Sci 122:499-504. 2009
    ..In the accompanying paper by Kuehnel et al. (doi:10.1242/jcs.034207), more evidence is provided in support of this model from the analysis of actin assembly at the plasma membrane of intact macrophages...
  4. doi request reprint Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion
    Sabrina Marion
    Department of Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, Germany
    Traffic 12:421-37. 2011
    ..Live-cell imaging analysis supported the notion that ezrin is necessary for the fusogenic process itself, promoting the transfer of the lysosome content into the phagosomal lumen...
  5. doi request reprint Role of lipids in killing mycobacteria by macrophages: evidence for NF-kappaB-dependent and -independent killing induced by different lipids
    Maximiliano Gabriel Gutierrez
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany
    Cell Microbiol 11:406-20. 2009
    ..smegmatis, M. avium and M. tuberculosis H37Rv. Our results argue that both NF-kappaB-dependent and -independent mechanisms are involved in macrophage killing of mycobacteria and that both mechanisms can be enhanced by selected lipids...
  6. doi request reprint Golgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin
    Anna Wähe
    European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany
    J Cell Sci 123:2502-11. 2010
    ..We propose a pathway mediated by sortilin by which selected lysosomal proteins are transported to the phagosome along a Golgi-dependent route during the maturation of phagosomes...
  7. ncbi request reprint cAMP synthesis and degradation by phagosomes regulate actin assembly and fusion events: consequences for mycobacteria
    Stefanos A Kalamidas
    EMBL, Postfach 102209, 69117 Heidelberg, Germany
    J Cell Sci 119:3686-94. 2006
    ..We argue that the phagosome cAMP-PKA system behaves as a molecular switch that regulates phagosome actin and maturation in macrophages...
  8. doi request reprint Sphingosine-1-phosphate receptors stimulate macrophage plasma-membrane actin assembly via ADP release, ATP synthesis and P2X7R activation
    Mark P Kuehnel
    EMBL, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Cell Sci 122:505-12. 2009
    ..The overall model we present is strongly supported by the data presented in the accompanying paper that focuses on latex bead phagosomes...
  9. pmc Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
    Paras K Anand
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 5:e10136. 2010
    ..Exosomes released from mycobacteria-infected cells have recently been shown to be pro-inflammatory. A prominent host molecule that is found within these exosomes is Hsp70, a member of the heat-shock family of proteins...
  10. ncbi request reprint Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies
    Holger Kress
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Phys Rev E Stat Nonlin Soft Matter Phys 71:061927. 2005
    ..The time delay between binding and response was on average 2 s...
  11. pmc Modelling phagosomal lipid networks that regulate actin assembly
    Mark Kühnel
    EMBL, Postfach 102209, 69117 Heidelberg, Germany
    BMC Syst Biol 2:107. 2008
    ..Hence, addition of ATP, lipids, and actin to the system alter the steady-state composition of the phagosomal membrane at the same time that the actin nucleation is initiated. Our aim was to model all these processes in parallel...
  12. ncbi request reprint NF-kappa B activation controls phagolysosome fusion-mediated killing of mycobacteria by macrophages
    Maximiliano Gabriel Gutierrez
    European Molecular Biology Laboratory, Heidelberg, Germany
    J Immunol 181:2651-63. 2008
    ..smegmatis phagosomes and reduced killing. Thus, the established role of NF-kappaB in the innate immune response can now be expanded to include regulation of membrane trafficking during infection...
  13. ncbi request reprint A role for the small GTPase Rab21 in the early endocytic pathway
    Jeremy C Simpson
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    J Cell Sci 117:6297-311. 2004
    ..Collectively, our data provide the first characterisation of Rab21 function in early endosome dynamics...
  14. pmc Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation
    Dominique T Brandt
    Institute of Pharmacology, University of Heidelberg, Heidelberg, Germany
    J Cell Biol 178:193-200. 2007
    ..Thus, we identify IQGAP1 as a novel component involved in the regulation of phagocytosis by mediating the localization of the actin filament nucleator Dia1...
  15. doi request reprint Porins facilitate nitric oxide-mediated killing of mycobacteria
    Daniela Leite Fabrino
    European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany
    Microbes Infect 11:868-75. 2009
    ..Our data provide evidence that porins render mycobacteria vulnerable to killing by reactive nitrogen intermediates within phagosomes probably by facilitating uptake of NO across the mycobacterial outer membrane...
  16. pmc Fusion between phagosomes, early and late endosomes: a role for actin in fusion between late, but not early endocytic organelles
    Rune Kjeken
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 15:345-58. 2004
    ..We propose that actin assembled by phagosomes or late endocytic organelles can provide tracks for fusion-partner organelles to move vectorially toward them, via membrane-bound myosins, to facilitate fusion...
  17. ncbi request reprint On phagosome individuality and membrane signalling networks
    Gareth Griffiths
    EMBL, Meyerhofstrasse 1, Postfach 102209, 69117 Heidelberg, Germany
    Trends Cell Biol 14:343-51. 2004
    ..Here, I argue that each phagosome is an individual entity whose behaviour depends on a finite number of stable equilibrium states in its membrane signalling networks...
  18. ncbi request reprint RanGTP mediates nuclear pore complex assembly
    Tobias C Walther
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 424:689-94. 2003
    ..The data demonstrate that RanGTP triggers distinct steps of NPC assembly, and suggest a mechanism for the spatial restriction of NPC assembly to the surface of chromatin...
  19. pmc The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions
    Boyan K Garvalov
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    J Cell Biol 161:33-9. 2003
    ..However, using RNAi ablation, we demonstrate that zyxin is required to recruit Tes, as well as Mena and VASP, but not vinculin or paxillin, to focal adhesions...
  20. pmc TNF-alpha-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells
    Irina Treede
    Department of Gastroenterology, University of Heidelberg, Heidelberg, Germany
    BMC Gastroenterol 9:53. 2009
    ..We investigate here in more detail the hypothesis that the exogenous application of PC has anti-inflammatory properties...
  21. pmc Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes
    Hélène Defacque
    European Molecular Biology Laboratory, 69012 Heidelberg, Germany
    Mol Biol Cell 13:1190-202. 2002
    ..We suggest that the ezrin-dependent actin assembly on the LBP membrane may require active turnover of D4 and D5 PIPs on the organelle membrane...
  22. doi request reprint Preparation of cells and tissues for immuno EM
    Paul Webster
    Cell Biology and Biophysics Unit, EMBL, Heidelberg, Meyerhofstrasse 69117, Germany
    Methods Cell Biol 88:45-58. 2008
    ..Given the space limitation, the fine details necessary to apply these methods have been successfully omitted and will have to be obtained from the technical references we provide...
  23. doi request reprint Vaccinia virus lacking A17 induces complex membrane structures composed of open membrane sheets
    Petr Chlanda
    European Molecular Biology Laboratory, Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
    Arch Virol 156:1647-53. 2011
    ..Tubular, vesicular and so far not described onion-shaped membranes, which consisted of open membrane sheets, were seen. Thus, the data show that membrane rupture occurs independently of the A17 protein...
  24. ncbi request reprint On the killing of mycobacteria by macrophages
    Luisa Jordao
    Molecular Pathogenesis Centre, Unit of Retrovirus and Associated Infections, Faculty of Pharmacy, University of Lisbon, Av Forcas Armadas, 1600 083 Lisbon, Portugal
    Cell Microbiol 10:529-48. 2008
    ....
  25. pmc Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue
    Michael K Leverentz
    Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom
    Plant Physiol 130:273-83. 2002
    ....
  26. ncbi request reprint A simpler way of comparing the labelling densities of cellular compartments illustrated using data from VPARP and LAMP-1 immunogold labelling experiments
    Terry Mayhew
    School of Biomedical Sciences, Queen s Medical Centre, E Floor, University of Nottingham, Nottingham, NG7 2UH, UK
    Histochem Cell Biol 119:333-41. 2003
    ..This approach provides a simple and efficient way of comparing LDs in different compartments. Its utility is illustrated using data from VPARP and LAMP-1 labelling experiments...
  27. ncbi request reprint Phagocytosis: latex leads the way
    Michel Desjardins
    Departement de pathologie et biologie cellulaire, Universite de Montreal, CP 6128, Succ Centre Ville, H3C 3J7, Montreal, Canada
    Curr Opin Cell Biol 15:498-503. 2003
    ..These approaches have led to unique insights directly relevant for the understanding of the biology of intracellular pathogens and the ways by which they subvert their hosts...
  28. ncbi request reprint Selected lipids activate phagosome actin assembly and maturation resulting in killing of pathogenic mycobacteria
    Elsa Anes
    Molecular Pathogenesis Centre, Faculty of Pharmacy, University of Lisbon, Av Forcas Armadas, 1600 085 Lisbon, Portugal
    Nat Cell Biol 5:793-802. 2003
    ..avium. In contrast, the polyunsaturated sigma-3 lipids behaved differently and stimulated pathogen growth. Thus, lipids can be involved in both stimulatory and inhibitory signalling networks in the phagosomal membrane...
  29. ncbi request reprint Citron-N is a neuronal Rho-associated protein involved in Golgi organization through actin cytoskeleton regulation
    Paola Camera
    Dipartimento di Genetica, Biologia e Biochimica, Università Degli Studi di Torino Via Santena, 5 bis, Torino, Italy
    Nat Cell Biol 5:1071-8. 2003
    ..Together with recent data on endosomal dynamics, our results highlight the importance of organelle-specific Rho modulators for actin-dependent organelle organization and dynamics...
  30. ncbi request reprint Dynamic life and death interactions between Mycobacterium smegmatis and J774 macrophages
    Elsa Anes
    Molecular Pathogenesis Centre, Faculty of Pharmacy, University of Lisbon, Av Forcas Armadas, 1600 083 Lisbon, Portugal
    Cell Microbiol 8:939-60. 2006
    ..A mathematical model argues that periodic high and low cellular killing activity is more effective than is a continuous process...
  31. pmc Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity
    Holger Kress
    European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Proc Natl Acad Sci U S A 104:11633-8. 2007
    ..The measured filopodial retraction force-velocity relationship is in agreement with a model for force-dependent multiple motor kinetics...
  32. ncbi request reprint Severe periodontitis is associated with systemic inflammation and a dysmetabolic status: a case-control study
    Luigi Nibali
    Periodontology Unit, Eastman Dental Institute and Hospital, University College London, London, UK
    J Clin Periodontol 34:931-7. 2007
    ..The aim of this study was to investigate the association between severe periodontitis and increase in inflammatory and metabolic risk factors for cardiovascular disease...
  33. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
    ..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
  34. pmc Anti-inflammatory effects of phosphatidylcholine
    Irina Treede
    Department of Gastroenterology, University Hospital Heidelberg, INF 345, Heidelberg 69120, Germany
    J Biol Chem 282:27155-64. 2007
    ..Collectively, these results provide a molecular foundation for the clinical studies showing a beneficial effect of PC therapy in ulcerative colitis...