Holger Erfle

Summary

Affiliation: European Molecular Biology Laboratory
Country: Germany

Publications

  1. ncbi request reprint Reverse transfection on cell arrays for high content screening microscopy
    Holger Erfle
    MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Protoc 2:392-9. 2007
  2. ncbi request reprint High-throughput RNAi screening by time-lapse imaging of live human cells
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Methods 3:385-90. 2006
  3. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
  4. doi request reprint Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
    Holger Erfle
    Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
    J Biomol Screen 13:575-80. 2008
  5. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
  6. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
  7. pmc Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines
    Kathleen Börner
    Department of Infectious Diseases, Virology, Heidelberg University Hospital, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany, Cluster of Excellence CellNetworks, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany, Department of Medicine III, University Hospital Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany, Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany, Division Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany, BIOQUANT, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany and Department of Pediatric Oncology, Hematology, Immunology and Pulmonology, Heidelberg University Hospital, Im Neuenheimer Feld 350, D 69120 Heidelberg, Germany
    Nucleic Acids Res 41:e199. 2013
  8. ncbi request reprint Arrays of transfected mammalian cells for high content screening microscopy
    Holger Erfle
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:1-8. 2005
  9. ncbi request reprint siRNA cell arrays for high-content screening microscopy
    Holger Erfle
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 37:454-8, 460, 462. 2004
  10. ncbi request reprint Transfected cell microarrays: an efficient tool for high-throughput functional analysis
    Vytaute Starkuviene
    European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
    Expert Rev Proteomics 4:479-89. 2007

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Reverse transfection on cell arrays for high content screening microscopy
    Holger Erfle
    MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Protoc 2:392-9. 2007
    ..Different human cell lines that have been successfully transfected using the protocol are presented here. The present protocol has been applied to two genome-wide siRNA screens addressing mitosis and constitutive protein secretion...
  2. ncbi request reprint High-throughput RNAi screening by time-lapse imaging of live human cells
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Methods 3:385-90. 2006
    ..This modular platform is scalable and makes the power of time-lapse microscopy available for genome-wide RNAi screens...
  3. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  4. doi request reprint Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
    Holger Erfle
    Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
    J Biomol Screen 13:575-80. 2008
    ..We conclude that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays...
  5. ncbi request reprint An RNAi screening platform to identify secretion machinery in mammalian cells
    Jeremy C Simpson
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biotechnol 129:352-65. 2007
    ..Correlating these findings with other screens and bioinformatic information makes these candidates highly likely to be novel membrane traffic machinery components...
  6. ncbi request reprint A microscope-based screening platform for large-scale functional protein analysis in intact cells
    Urban Liebel
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 554:394-8. 2003
    ..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
  7. pmc Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines
    Kathleen Börner
    Department of Infectious Diseases, Virology, Heidelberg University Hospital, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany, Cluster of Excellence CellNetworks, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany, Department of Medicine III, University Hospital Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany, Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany, Division Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany, BIOQUANT, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany and Department of Pediatric Oncology, Hematology, Immunology and Pulmonology, Heidelberg University Hospital, Im Neuenheimer Feld 350, D 69120 Heidelberg, Germany
    Nucleic Acids Res 41:e199. 2013
    ..Our customizable reagents and avenues should broadly improve future in vitro and in vivo RNAi experiments in mammalian systems. ..
  8. ncbi request reprint Arrays of transfected mammalian cells for high content screening microscopy
    Holger Erfle
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Enzymol 404:1-8. 2005
    ..The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel...
  9. ncbi request reprint siRNA cell arrays for high-content screening microscopy
    Holger Erfle
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 37:454-8, 460, 462. 2004
    ..We demonstrate such an siRNA cell array in a microscope-based functional assay in living cells to determine the effect of various siRNA oligonucleotides against endogenous targets on cellular secretion...
  10. ncbi request reprint Transfected cell microarrays: an efficient tool for high-throughput functional analysis
    Vytaute Starkuviene
    European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
    Expert Rev Proteomics 4:479-89. 2007
    ..These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level...
  11. ncbi request reprint Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells
    Holger Erfle
    Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    Methods Mol Biol 360:155-61. 2007
    ..This method should facilitate drug target validation by small-interfering RNAs...
  12. ncbi request reprint High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol
    Michael Sturzl
    Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen Nuremberg, Schwabachanlage 10, D 91054 Erlangen, Germany
    Comb Chem High Throughput Screen 11:159-72. 2008
    ..Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol...
  13. pmc High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic
    Vytaute Starkuviene
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
    Genome Res 14:1948-56. 2004
    ..It will also serve as an example for similar microscope-based screens addressing different biological questions...
  14. pmc Automatic identification of subcellular phenotypes on human cell arrays
    Christian Conrad
    Intelligent Bioinformatics Systems, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Genome Res 14:1130-6. 2004
    ..Our classification method can be adapted to virtually any microscopic assay based on cell morphology, opening a wide range of applications including large-scale RNAi screening in human cells...