Christian Eggeling

Summary

Country: Germany

Publications

  1. doi request reprint STED microscopy of living cells--new frontiers in membrane and neurobiology
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Neurochem 126:203-12. 2013
  2. ncbi request reprint Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
    C Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Microsc Res Tech 70:1003-9. 2007
  3. ncbi request reprint Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Phys Chem A 110:2979-95. 2006
  4. ncbi request reprint Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
  5. ncbi request reprint Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction
    C Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Goettingen, Germany
    Curr Pharm Biotechnol 6:351-71. 2005
  6. ncbi request reprint Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
    Christian Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 6:791-804. 2005
  7. pmc Rapid analysis of Forster resonance energy transfer by two-color global fluorescence correlation spectroscopy: trypsin proteinase reaction
    Christian Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Goettingen, Germany
    Biophys J 89:605-18. 2005
  8. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
  9. pmc Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron
    Tanja Brakemann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 285:14603-9. 2010
  10. pmc 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochem J 402:35-42. 2007

Collaborators

Detail Information

Publications52

  1. doi request reprint STED microscopy of living cells--new frontiers in membrane and neurobiology
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Neurochem 126:203-12. 2013
    ....
  2. ncbi request reprint Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
    C Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Microsc Res Tech 70:1003-9. 2007
    ..Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range...
  3. ncbi request reprint Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Phys Chem A 110:2979-95. 2006
    ..The observations made are of strong relevance for and demand a careful choice of laser action in multicolor and FRET experiments, in particular when performed at or close to saturation...
  4. ncbi request reprint Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
    ..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...
  5. ncbi request reprint Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction
    C Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Goettingen, Germany
    Curr Pharm Biotechnol 6:351-71. 2005
    ....
  6. ncbi request reprint Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
    Christian Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 6:791-804. 2005
    ..Furthermore, the photostability of the higher-excited electronic states is strongly influenced by environmental conditions, such as polarity and temperature...
  7. pmc Rapid analysis of Forster resonance energy transfer by two-color global fluorescence correlation spectroscopy: trypsin proteinase reaction
    Christian Eggeling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Goettingen, Germany
    Biophys J 89:605-18. 2005
    ..The results were compared to those obtained by two-dimensional fluorescence intensity distribution analysis...
  8. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
    ..Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm...
  9. pmc Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron
    Tanja Brakemann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 285:14603-9. 2010
    ..Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process...
  10. pmc 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochem J 402:35-42. 2007
    ..The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs...
  11. pmc Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 95:2989-97. 2008
    ..We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules...
  12. doi request reprint A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
    Tanja Brakemann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Nat Biotechnol 29:942-7. 2011
    ..The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach...
  13. doi request reprint New fluorinated rhodamines for optical microscopy and nanoscopy
    Gyuzel Yu Mitronova
    Department of Nano Biophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen Germany, Fax 49 551 2012505
    Chemistry 16:4477-88. 2010
    ..We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments. ..
  14. ncbi request reprint Anatomy and dynamics of a supramolecular membrane protein cluster
    Jochen J Sieber
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 317:1072-6. 2007
    ....
  15. doi request reprint Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
    Tim Grotjohann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 478:204-8. 2011
    ..The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage...
  16. doi request reprint Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy
    Christian Ringemann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:612-24. 2008
    ..The study of ReISC not only results in a better understanding of a fluorescent label's photophysics, but the method is a possible approach to optimize fluorescence emission in experiments, where signal strength is a critical parameter...
  17. pmc Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 99:2686-94. 2010
    ..The method can be expanded to even more colors by choosing optimized dichroic mirrors and selecting marker molecules with negligible inhomogeneous emission broadening...
  18. pmc Structural basis for reversible photoswitching in Dronpa
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:13005-9. 2007
    ..We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore...
  19. ncbi request reprint Orientational and dynamical heterogeneity of rhodamine 6G terminally attached to a DNA helix revealed by NMR and single-molecule fluorescence spectroscopy
    Heike Neubauer
    Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, 37077, Gottingen, Germany
    J Am Chem Soc 129:12746-55. 2007
    ..From both methods, a consistent and detailed molecular description of the structural and dynamical heterogeneity is obtained...
  20. pmc Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 93:3285-90. 2007
    ..These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm...
  21. ncbi request reprint Nanoscale separation of molecular species based on their rotational mobility
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:21093-104. 2008
    ..Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells...
  22. doi request reprint Fluorescence nanoscopy by ground-state depletion and single-molecule return
    Jonas Fölling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:943-5. 2008
    ..Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach...
  23. ncbi request reprint Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
    Mariano Bossi
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nano Lett 8:2463-8. 2008
    ..The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques...
  24. doi request reprint Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
    Jonas Fölling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:321-6. 2008
    ..Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning...
  25. ncbi request reprint STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue
    Alf Honigmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Faraday Discuss 161:77-89; discussion 113-50. 2013
    ....
  26. doi request reprint Nanoscopy of living brain slices with low light levels
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Neuron 75:992-1000. 2012
    ....
  27. ncbi request reprint Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
    Miriam A Schwentker
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Microsc Res Tech 70:269-80. 2007
    ..The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds...
  28. doi request reprint Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 16:158-66. 2010
    ..g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy...
  29. doi request reprint Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)
    Marcel Leutenegger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 20:5243-63. 2012
    ..Together with the estimated axial confinement of about 55 nm, our TIRF-STED nanoscope achieved an almost isotropic and less than 1 attoliter small all-optically induced measurement volume...
  30. doi request reprint STED with wavelengths closer to the emission maximum
    Giuseppe Vicidomini
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 20:5225-36. 2012
    ..The method is exemplified by imaging immunolabeled features in mammalian cells with an up to 3-fold increased STED efficiency compared to that encountered in standard STED nanoscopy implementations...
  31. doi request reprint Far-field autofluorescence nanoscopy
    Jakob Bierwagen
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gttingen, Germany
    Nano Lett 10:4249-52. 2010
    ..The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts...
  32. doi request reprint Triplet-relaxation microscopy with bunched pulsed excitation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Photochem Photobiol Sci 8:481-5. 2009
    ..Reaching almost T-Rex conditions this excitation scheme mimics fast scanning of the illumination beam and has the potential to improve a whole range of analytical tools that suffer from photobleaching and low signal levels...
  33. doi request reprint Nanoscopy with more than 100,000 'doughnuts'
    Andriy Chmyrov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nat Methods 10:737-40. 2013
    ..We super-resolved living cells in 120 μm × 100 μm-sized fields of view in <1 s using 116,000 such doughnuts. ..
  34. doi request reprint FCS in STED microscopy: studying the nanoscale of lipid membrane dynamics
    Veronika Mueller
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Methods Enzymol 519:1-38. 2013
    ..STED-FCS is a highly sensitive and exceptional tool to study the membrane organization by introducing a new class of nanoscale biomolecular studies...
  35. pmc rsEGFP2 enables fast RESOLFT nanoscopy of living cells
    Tim Grotjohann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    elife 1:e00248. 2012
    ..We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001...
  36. pmc Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids
    Steffen J Sahl
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 107:6829-34. 2010
    ..Our experimental approach demonstrates that fast molecular movements can be tracked with minimal invasion, which can reveal new important details of cellular nano-organization...
  37. pmc Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    Michael Hofmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:17565-9. 2005
    ..Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization...
  38. pmc Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment
    Alf Honigmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nat Struct Mol Biol 20:679-86. 2013
    ..Our results suggest that PIP2 clusters organized by syntaxin-1 act as molecular beacons for vesicle docking, with the subsequent Ca(2+) influx bringing the vesicle membrane close enough for membrane fusion...
  39. doi request reprint Analytical description of STED microscopy performance
    Marcel Leutenegger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 18:26417-29. 2010
    ..Particular emphasis is placed on fluorescence fluctuation methods such as correlation spectroscopy (FCS) using STED...
  40. doi request reprint Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light
    Kyu Young Han
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nano Lett 9:3323-9. 2009
    ..Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences...
  41. ncbi request reprint Major signal increase in fluorescence microscopy through dark-state relaxation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 4:81-6. 2007
    ..The signal gain was observed both for one- and two-photon excitation. Obeying dark or triplet state relaxation in the illumination process signifies a major step toward imaging with low photobleaching and strong fluorescence fluxes...
  42. ncbi request reprint Monitoring triplet state dynamics with fluorescence correlation spectroscopy: Bias and correction
    Andreas Schönle
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Gottingen, Germany
    Microsc Res Tech 77:528-36. 2014
    ..Importantly, our approach can be readily generalized to most other FCS experiments that determine intensity dependent kinetic parameters. Microsc. Res. Tech. 77:528-536, 2014. © 2014 Wiley Periodicals, Inc. ..
  43. doi request reprint Sharper low-power STED nanoscopy by time gating
    Giuseppe Vicidomini
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Nat Methods 8:571-3. 2011
    ..This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells...
  44. ncbi request reprint Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
    Lars Kastrup
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Phys Rev Lett 94:178104. 2005
    ..Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations...
  45. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    ..The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences...
  46. ncbi request reprint Breaking the diffraction barrier in fluorescence microscopy by optical shelving
    Stefan Bretschneider
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Phys Rev Lett 98:218103. 2007
    ..The presence of dark states in virtually any fluorescent molecule opens up a new venue for far-field microscopy with resolution that is no longer limited by diffraction...
  47. pmc Structure and mechanism of the reversible photoswitch of a fluorescent protein
    Martin Andresen
    Department of NanoBiophotonics, Theoretical and Computational Biophysics, and X Ray Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:13070-4. 2005
    ..Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording...
  48. pmc Fluorescence intensity and lifetime distribution analysis: toward higher accuracy in fluorescence fluctuation spectroscopy
    Kaupo Palo
    Evotec OAI, D 22525, Hamburg, Germany
    Biophys J 83:605-18. 2002
    ....
  49. ncbi request reprint Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
    Jerker Widengren
    Experimental Biomolecular Physics, Department of Applied Physics, AlbaNova University Center, Royal Institute of Technology, SE 10691 Stockholm, Sweden
    J Phys Chem A 111:429-40. 2007
    ....
  50. ncbi request reprint Dual-color total internal reflection fluorescence cross-correlation spectroscopy
    Marcel Leutenegger
    J Biomed Opt 11:040502. 2006
    ..Further improvements have been achieved through global analysis of the spectroscopic data...
  51. ncbi request reprint Experiences in implementing uHTS--cutting edge technology meets the real world
    Philip Gribbon
    Pfizer Global Research and Development, Lead Discovery Technologies, IPC 580, Ramsgate Road, Sandwich CT13 9NJ, UK
    Curr Drug Discov Technol 1:27-35. 2004
    ..In this article we will outline the benefits of the approach taken at Pfizer, Sandwich, and introduce the Mark-II EVOscreen platform, illustrating the potential but also possible pitfalls of HTS miniaturisation...
  52. pmc Two-color far-field fluorescence nanoscopy
    Gerald Donnert
    Biophys J 92:L67-9. 2007
    ..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...