Research Topics
Species | Christian EggelingSummaryCountry: Germany Publications
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Publications
Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reactionC Eggeling
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Goettingen, Germany
Curr Pharm Biotechnol 6:351-71. 2005....- Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentrationC Eggeling
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
Microsc Res Tech 70:1003-9. 2007..Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range... 
Direct observation of the nanoscale dynamics of membrane lipids in a living cellChristian Eggeling
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Nature 457:1159-62. 2009..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...- Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurementsChristian Eggeling
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
J Phys Chem A 110:2979-95. 2006..The observations made are of strong relevance for and demand a careful choice of laser action in multicolor and FRET experiments, in particular when performed at or close to saturation... 
Rapid analysis of Forster resonance energy transfer by two-color global fluorescence correlation spectroscopy: trypsin proteinase reactionChristian Eggeling
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Goettingen, Germany
Biophys J 89:605-18. 2005..The results were compared to those obtained by two-dimensional fluorescence intensity distribution analysis...- Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopyChristian Eggeling
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
Chemphyschem 6:791-804. 2005..Furthermore, the photostability of the higher-excited electronic states is strongly influenced by environmental conditions, such as polarity and temperature... - Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopyMartin Andresen
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Nat Biotechnol 26:1035-40. 2008..Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm... - Molecular basis of the light-driven switching of the photochromic fluorescent protein PadronTanja Brakemann
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
J Biol Chem 285:14603-9. 2010..Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process... - Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopyAndre C Stiel
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Biophys J 95:2989-97. 2008..We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules... - A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switchingTanja Brakemann
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
Nat Biotechnol 29:942-7. 2011..The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach... - Diffraction-unlimited all-optical imaging and writing with a photochromic GFPTim Grotjohann
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Nature 478:204-8. 2011..The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage... - Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopyChristian Ringemann
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
Chemphyschem 9:612-24. 2008..The study of ReISC not only results in a better understanding of a fluorescent label's photophysics, but the method is a possible approach to optimize fluorescence emission in experiments, where signal strength is a critical parameter... - Structural basis for reversible photoswitching in DronpaMartin Andresen
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Proc Natl Acad Sci U S A 104:13005-9. 2007..We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore... - Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelengthIlaria Testa
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Biophys J 99:2686-94. 2010..The method can be expanded to even more colors by choosing optimized dichroic mirrors and selecting marker molecules with negligible inhomogeneous emission broadening... - 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variantsAndre C Stiel
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Biochem J 402:35-42. 2007..The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs... - Orientational and dynamical heterogeneity of rhodamine 6G terminally attached to a DNA helix revealed by NMR and single-molecule fluorescence spectroscopyHeike Neubauer
Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, 37077, Gottingen, Germany
J Am Chem Soc 129:12746-55. 2007..From both methods, a consistent and detailed molecular description of the structural and dynamical heterogeneity is obtained... - Nanoscale separation of molecular species based on their rotational mobilityIlaria Testa
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Opt Express 16:21093-104. 2008..Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells... - Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular speciesMariano Bossi
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Nano Lett 8:2463-8. 2008..The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques... - Anatomy and dynamics of a supramolecular membrane protein clusterJochen J Sieber
Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Science 317:1072-6. 2007.... - Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasersJonas Fölling
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Chemphyschem 9:321-6. 2008..Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning... - Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emittersAlexander Egner
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Biophys J 93:3285-90. 2007..These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm... - Fluorescence nanoscopy by ground-state depletion and single-molecule returnJonas Fölling
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
Nat Methods 5:943-5. 2008..Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach... - Nanoscopy of living brain slices with low light levelsIlaria Testa
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Neuron 75:992-1000. 2012.... - Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopyKirill Kolmakov
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Chemistry 16:158-66. 2010..g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy... - Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitchingMiriam A Schwentker
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Microsc Res Tech 70:269-80. 2007..The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds... - STED with wavelengths closer to the emission maximumGiuseppe Vicidomini
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Opt Express 20:5225-36. 2012..The method is exemplified by imaging immunolabeled features in mammalian cells with an up to 3-fold increased STED efficiency compared to that encountered in standard STED nanoscopy implementations... - Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)Marcel Leutenegger
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Opt Express 20:5243-63. 2012..Together with the estimated axial confinement of about 55 nm, our TIRF-STED nanoscope achieved an almost isotropic and less than 1 attoliter small all-optically induced measurement volume... - Triplet-relaxation microscopy with bunched pulsed excitationGerald Donnert
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
Photochem Photobiol Sci 8:481-5. 2009..Reaching almost T-Rex conditions this excitation scheme mimics fast scanning of the illumination beam and has the potential to improve a whole range of analytical tools that suffer from photobleaching and low signal levels... - Far-field autofluorescence nanoscopyJakob Bierwagen
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gttingen, Germany
Nano Lett 10:4249-52. 2010..The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts... - rsEGFP2 enables fast RESOLFT nanoscopy of living cellsTim Grotjohann
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
elife 1:e00248. 2012..We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001... - Fast molecular tracking maps nanoscale dynamics of plasma membrane lipidsSteffen J Sahl
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Proc Natl Acad Sci U S A 107:6829-34. 2010..Our experimental approach demonstrates that fast molecular movements can be tracked with minimal invasion, which can reveal new important details of cellular nano-organization... - Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteinsMichael Hofmann
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, , Germany
Proc Natl Acad Sci U S A 102:17565-9. 2005..Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization... - Analytical description of STED microscopy performanceMarcel Leutenegger
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Opt Express 18:26417-29. 2010..Particular emphasis is placed on fluorescence fluctuation methods such as correlation spectroscopy (FCS) using STED... - Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave lightKyu Young Han
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
Nano Lett 9:3323-9. 2009..Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences... - Major signal increase in fluorescence microscopy through dark-state relaxationGerald Donnert
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
Nat Methods 4:81-6. 2007..The signal gain was observed both for one- and two-photon excitation. Obeying dark or triplet state relaxation in the illumination process signifies a major step toward imaging with low photobleaching and strong fluorescence fluxes... - Fluorescence fluctuation spectroscopy in subdiffraction focal volumesLars Kastrup
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, , Germany
Phys Rev Lett 94:178104. 2005..Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations... - Sharper low-power STED nanoscopy by time gatingGiuseppe Vicidomini
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
Nat Methods 8:571-3. 2011..This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells... - Macromolecular-scale resolution in biological fluorescence microscopyGerald Donnert
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, , Germany
Proc Natl Acad Sci U S A 103:11440-5. 2006..The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences... - FCS in STED microscopy: studying the nanoscale of lipid membrane dynamicsVeronika Mueller
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Methods Enzymol 519:1-38. 2013..STED-FCS is a highly sensitive and exceptional tool to study the membrane organization by introducing a new class of nanoscale biomolecular studies... - Breaking the diffraction barrier in fluorescence microscopy by optical shelvingStefan Bretschneider
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
Phys Rev Lett 98:218103. 2007..The presence of dark states in virtually any fluorescent molecule opens up a new venue for far-field microscopy with resolution that is no longer limited by diffraction... - Structure and mechanism of the reversible photoswitch of a fluorescent proteinMartin Andresen
Department of NanoBiophotonics, Theoretical and Computational Biophysics, and X-Ray Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
Proc Natl Acad Sci U S A 102:13070-4. 2005..Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording... - Fluorescence intensity and lifetime distribution analysis: toward higher accuracy in fluorescence fluctuation spectroscopyKaupo Palo
Evotec OAI, D-22525, Hamburg, Germany
Biophys J 83:605-18. 2002.... - Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopyJerker Widengren
Experimental Biomolecular Physics, Department of Applied Physics, AlbaNova University Center, Royal Institute of Technology, SE 10691 Stockholm, Sweden
J Phys Chem A 111:429-40. 2007.... - Experiences in implementing uHTS--cutting edge technology meets the real worldPhilip Gribbon
Pfizer Global Research and Development, Lead Discovery Technologies, IPC 580, Ramsgate Road, Sandwich CT13 9NJ, UK
Curr Drug Discov Technol 1:27-35. 2004..In this article we will outline the benefits of the approach taken at Pfizer, Sandwich, and introduce the Mark-II EVOscreen platform, illustrating the potential but also possible pitfalls of HTS miniaturisation... - Dual-color total internal reflection fluorescence cross-correlation spectroscopyMarcel Leutenegger
J Biomed Opt 11:040502. 2006..Further improvements have been achieved through global analysis of the spectroscopic data... - Two-color far-field fluorescence nanoscopyGerald Donnert
Biophys J 92:L67-9. 2007..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...