Research Topics
Species | Henrik DaubSummaryCountry: Germany Publications
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Publications
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycleHenrik Daub
Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell 31:438-48. 2008..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
Characterisation of kinase-selective inhibitors by chemical proteomicsHenrik Daub
Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinried, Germany
Biochim Biophys Acta 1754:183-90. 2005..Importantly, these chemical proteomic methods permit the direct analysis of kinase inhibitor selectivity in biological systems and have led to new insights into the cellular modes of action of kinase-selective small molecule antagonists...
Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometryJosef Wissing
Department of Cell Biology, Helmholtz Centre for Infection Research HZI, Inhoffenstrasse 7, 38124 Braunschweig, Germany
Mol Cell Proteomics 6:537-47. 2007....
An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signalingThiemo B Schreiber
Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell Proteomics 9:1047-62. 2010....
Large-scale proteomics analysis of the human kinomeFelix S Oppermann
Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Mol Cell Proteomics 8:1751-64. 2009..In conclusion, the straightforward experimental procedures described here enable different implementations of kinase-selective proteomics with considerable potential for future signal transduction and kinase drug target analysis...
Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomicsCuiping Pan
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
Mol Cell Proteomics 8:2796-808. 2009..Our assay is streamlined and generic and could become a useful tool in kinase drug development...
Quantitative analysis of kinase-proximal signaling in lipopolysaccharide-induced innate immune responseKirti Sharma
Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
J Proteome Res 9:2539-49. 2010..Thus, our data expands previous knowledge for functional analyses of innate immune response...
Proteomics strategy for quantitative protein interaction profiling in cell extractsKirti Sharma
Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Nat Methods 6:741-4. 2009..We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands...
Proteomics analysis of cellular imatinib targets and their candidate downstream effectorsSusanne B Breitkopf
Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
J Proteome Res 9:6033-43. 2010..Our approach is rather generic and may have various applications in kinase drug discovery...
Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progressionKalyan Dulla
Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell Proteomics 9:1167-81. 2010..Thus, the results of this study provide a valuable resource for cell biologists and provide insight into the system properties of the mitotic phosphokinome...
Combination of chemical genetics and phosphoproteomics for kinase signaling analysis enables confident identification of cellular downstream targetsFelix S Oppermann
Cell Signaling Group, Department of Molecular Biology, 82152 Martinsried, Germany
Mol Cell Proteomics 11:O111.012351. 2012..We anticipate the described strategies to be of general utility for systematic and confident identification of cellular protein kinase substrates...
Global analysis of phosphoproteome regulation by the Ser/Thr phosphatase Ppt1 in Saccharomyces cerevisiaeThiemo B Schreiber
Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
J Proteome Res 11:2397-408. 2012..These results demonstrate the utility of large-scale and quantitative phosphoproteomics to identify cellular sites of serine/threonine phosphatase action in an unbiased manner...
Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cellsChristoph Weber
Project Group Cell Signaling, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
J Proteomics 75:1343-56. 2012..Taken together, our data suggest that cellular perturbation of SFKs and/or Btk translates into rather specific signal transduction inhibition, which in turn contributes to the antileukemic activity of erlotinib and gefitinib in AML...
Quantitative phosphoproteomics--an emerging key technology in signal-transduction researchThiemo B Schreiber
Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Proteomics 8:4416-32. 2008..Here, we provide an overview of these exciting developments and their potential to transform signal-transduction research into a technology-driven, high-throughput science...
Glycoprotein capture and quantitative phosphoproteomics indicate coordinated regulation of cell migration upon lysophosphatidic acid stimulationNina Mäusbacher
Project Group Cell Signaling, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, Germany
Mol Cell Proteomics 9:2337-53. 2010..In conclusion, the glycoproteome phosphoproteomics strategy described here sheds light on incompletely understood mechanisms in LPA-induced cell migratory behavior...
Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomicsPhilipp Mertins
Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell Proteomics 7:1763-77. 2008..Importantly the MS-based strategies described here are entirely generic and can be used to address the poorly understood aspects of cellular PTP function...
AXL is a potential target for therapeutic intervention in breast cancer progressionYi Xiang Zhang
Max Planck Institute of Biochemistry, Martinsried, Germany
Cancer Res 68:1905-15. 2008....
Proteome-wide analysis of temporal phosphorylation dynamics in lysophosphatidic acid-induced signalingNina Mäusbacher
Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Proteomics 12:3485-98. 2012..Finally, we detected regulation of functionally characterized phosphorylation sites not yet implicated in LPA signaling, which may foster a better understanding how LPA regulates cellular physiology on the molecular level...
Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core proteinHenrik Daub
Axxima Pharmaceuticals AG, 82152 Martinsried, Germany
J Virol 76:8124-37. 2002....
Evaluation of kinase inhibitor selectivity by chemical proteomicsHenrik Daub
Axxima Pharmaceuticals AG, Munchen, Germany
Assay Drug Dev Technol 2:215-24. 2004....
Cellular targets of gefitinibDirk Brehmer
Axxima Pharmaceuticals AG, Munich, Germany
Cancer Res 65:379-82. 2005..Our data suggest alternative cellular modes of action for gefitinib and provide rationales for the development of related drugs...
Exploiting features of adenovirus replication to support mammalian kinase productionMatt Cotten
Axxima Pharmaceuticals AG, Max Lebsche Platz 32, 81377 Munchen, Germany
Nucleic Acids Res 31:e128. 2003..Thus, the utility of adenovirus-mediated enzyme expression as a versatile alternative to established protein production technologies is demonstrated...
Chemical proteomic analysis reveals alternative modes of action for pyrido[2,3-d]pyrimidine kinase inhibitorsJosef Wissing
Axxima Pharmaceuticals AG, , Germany
Mol Cell Proteomics 3:1181-93. 2004..Thus, our data demonstrate the utility of proteomic methods employing immobilized kinase inhibitors for identifying new targets linked to previously unrecognized therapeutic applications...
An efficient proteomics method to identify the cellular targets of protein kinase inhibitorsKlaus Godl
Axxima Pharmaceuticals AG, Max-Lebsche-Platz 32, 81377 Munich, Germany
Proc Natl Acad Sci U S A 100:15434-9. 2003..Based on the procedures described here, efficient affinity purification techniques can be developed for other protein kinase inhibitors, providing crucial information about their cellular modes of action...
Proteome-wide identification of cellular targets affected by bisindolylmaleimide-type protein kinase C inhibitorsDirk Brehmer
Axxima Pharmaceuticals AG, Max-Lebsche-Platz 32, 81377 Munich, Germany
Mol Cell Proteomics 3:490-500. 2004....
Proteomic analysis of kinase inhibitor selectivity and functionKlaus Godl
Axxima Pharmaceuticals AG, Max-Lebsche-Platz 32, , Germany
Cell Cycle 3:393-5. 2004....
Characterization of a conserved structural determinant controlling protein kinase sensitivity to selective inhibitorsStephanie Blencke
Axxima Pharmaceuticals AG, Max-Lebsche-Platz 32, 81377 Munich, Germany
Chem Biol 11:691-701. 2004..Our data establish a conserved structural determinant of protein kinase sensitivity relevant for both signal transduction research and drug development...
Strategies to overcome resistance to targeted protein kinase inhibitorsHenrik Daub
Axxima Pharmaceuticals AG, Max Lebsche Platz 32, 81377 Munchen, Germany
Nat Rev Drug Discov 3:1001-10. 2004....
Proteomic characterization of the angiogenesis inhibitor SU6668 reveals multiple impacts on cellular kinase signalingKlaus Godl
Axxima Pharmaceuticals AG, Munich, Germany
Cancer Res 65:6919-26. 2005....
