Margitta Dathe

Summary

Country: Germany

Publications

  1. ncbi request reprint General aspects of peptide selectivity towards lipid bilayers and cell membranes studied by variation of the structural parameters of amphipathic helical model peptides
    Margitta Dathe
    Research Institute of Molecular Pharmacology, Berlin, Germany
    Biochim Biophys Acta 1558:171-86. 2002
  2. ncbi request reprint Antimicrobial activity of arginine- and tryptophan-rich hexapeptides: the effects of aromatic clusters, D-amino acid substitution and cyclization
    A Wessolowski
    Institute of Molecular Pharmacology, Robert Roessle Str 10, D 13125 Berlin, Germany
    J Pept Res 64:159-69. 2004
  3. ncbi request reprint Cyclization increases the antimicrobial activity and selectivity of arginine- and tryptophan-containing hexapeptides
    Margitta Dathe
    Institute of Molecular Pharmacology, Robert Roessle Strasse 10, D 13125 Berlin, Germany
    Biochemistry 43:9140-50. 2004
  4. ncbi request reprint Proline at position 14 of alamethicin is essential for hemolytic activity, catecholamine secretion from chromaffin cells and enhanced metabolic activity in endothelial cells
    M Dathe
    Forschungsinstitut fur Molekulare Pharmakologie, Alfred Kowalke Strasse 4, D 10315 Berlin, Germany
    Biochim Biophys Acta 1370:175-83. 1998
  5. ncbi request reprint Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells
    M Dathe
    Research Institute of Molecular Pharmacology, Alfred Kowalke Strasse 4, D 10315, Berlin, Germany
    Biochim Biophys Acta 1462:71-87. 1999
  6. ncbi request reprint Optimization of the antimicrobial activity of magainin peptides by modification of charge
    M Dathe
    Research Institute of Molecular Pharmacology, Robert Rossle Str 10, D 13125 Berlin, Germany
    FEBS Lett 501:146-50. 2001
  7. pmc Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum
    Mojtaba Bagheri
    Leibniz Institute of Molecular Pharmacology, Berlin, Germany
    Antimicrob Agents Chemother 53:1132-41. 2009
  8. ncbi request reprint Structures of cyclic, antimicrobial peptides in a membrane-mimicking environment define requirements for activity
    Christian Appelt
    Leibnizinstitut für Molekulare Pharmakologie FMP, Robert Rossle Str 10, D 13125 Berlin, Germany
    J Pept Sci 14:524-7. 2008
  9. doi request reprint Mode of action of cationic antimicrobial peptides defines the tethering position and the efficacy of biocidal surfaces
    Mojtaba Bagheri
    Leibniz Institute of Molecular Pharmacology, Robert Roessle Strasse 10, Berlin, Germany
    Bioconjug Chem 23:66-74. 2012
  10. ncbi request reprint The interaction of arginine- and tryptophan-rich cyclic hexapeptides with Escherichia coli membranes
    Christof Junkes
    Leibniz Institute of Molecular Pharmacology, Robert Rossle Str 10, 13125 Berlin, Germany
    J Pept Sci 14:535-43. 2008

Collaborators

Detail Information

Publications33

  1. ncbi request reprint General aspects of peptide selectivity towards lipid bilayers and cell membranes studied by variation of the structural parameters of amphipathic helical model peptides
    Margitta Dathe
    Research Institute of Molecular Pharmacology, Berlin, Germany
    Biochim Biophys Acta 1558:171-86. 2002
    ..However, their influence on cell selectivity was limited. A threshold value of hydrophobicity seems to exist which restricted the activity modifying potential of mu and Phi on both lipid bilayers and cell membranes...
  2. ncbi request reprint Antimicrobial activity of arginine- and tryptophan-rich hexapeptides: the effects of aromatic clusters, D-amino acid substitution and cyclization
    A Wessolowski
    Institute of Molecular Pharmacology, Robert Roessle Str 10, D 13125 Berlin, Germany
    J Pept Res 64:159-69. 2004
    ....
  3. ncbi request reprint Cyclization increases the antimicrobial activity and selectivity of arginine- and tryptophan-containing hexapeptides
    Margitta Dathe
    Institute of Molecular Pharmacology, Robert Roessle Strasse 10, D 13125 Berlin, Germany
    Biochemistry 43:9140-50. 2004
    ..The results suggest that cyclization of small peptides of an appropriate amino acid composition may serve as a promising strategy in the design of antimicrobial peptides...
  4. ncbi request reprint Proline at position 14 of alamethicin is essential for hemolytic activity, catecholamine secretion from chromaffin cells and enhanced metabolic activity in endothelial cells
    M Dathe
    Forschungsinstitut fur Molekulare Pharmakologie, Alfred Kowalke Strasse 4, D 10315 Berlin, Germany
    Biochim Biophys Acta 1370:175-83. 1998
    ..The results indicate that proline at position 14 of the native peptide is essential for the interaction with different membrane systems...
  5. ncbi request reprint Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells
    M Dathe
    Research Institute of Molecular Pharmacology, Alfred Kowalke Strasse 4, D 10315, Berlin, Germany
    Biochim Biophys Acta 1462:71-87. 1999
    ..Finally, the knowledge about the role of peptide structural parameters is applied to understand the different activity spectra of natural membrane-lytic peptides...
  6. ncbi request reprint Optimization of the antimicrobial activity of magainin peptides by modification of charge
    M Dathe
    Research Institute of Molecular Pharmacology, Robert Rossle Str 10, D 13125 Berlin, Germany
    FEBS Lett 501:146-50. 2001
    ..H(hd) values above a certain threshold led to effective permeabilization of all lipid systems and even compensated for the activity-reducing effect of charge increase on highly negatively charged membranes...
  7. pmc Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum
    Mojtaba Bagheri
    Leibniz Institute of Molecular Pharmacology, Berlin, Germany
    Antimicrob Agents Chemother 53:1132-41. 2009
    ..In conclusion, reasonable antimicrobial activity of surface-bound peptides requires the optimization of the coupling parameters, with the length of the spacer and the amount of target-accessible peptide being the most important factors...
  8. ncbi request reprint Structures of cyclic, antimicrobial peptides in a membrane-mimicking environment define requirements for activity
    Christian Appelt
    Leibnizinstitut für Molekulare Pharmakologie FMP, Robert Rossle Str 10, D 13125 Berlin, Germany
    J Pept Sci 14:524-7. 2008
    ..The peptides do, however, all adopt an amphiphilic structure. A comparison between the structures helps to define the requirements for the activity of these peptides...
  9. doi request reprint Mode of action of cationic antimicrobial peptides defines the tethering position and the efficacy of biocidal surfaces
    Mojtaba Bagheri
    Leibniz Institute of Molecular Pharmacology, Robert Roessle Strasse 10, Berlin, Germany
    Bioconjug Chem 23:66-74. 2012
    ..The relationship between the mechanism of action and position of immobilization is highly relevant for the establishment of a general approach to obtain efficient biocidal solid matrices loaded with CAPs...
  10. ncbi request reprint The interaction of arginine- and tryptophan-rich cyclic hexapeptides with Escherichia coli membranes
    Christof Junkes
    Leibniz Institute of Molecular Pharmacology, Robert Rossle Str 10, 13125 Berlin, Germany
    J Pept Sci 14:535-43. 2008
    ..coli. In addition to membrane permeabilization, the data provided evidence for the involvement of other mechanisms in growth inhibition and killing of bacteria...
  11. pmc Interaction of W-substituted analogs of cyclo-RRRWFW with bacterial lipopolysaccharides: the role of the aromatic cluster in antimicrobial activity
    Mojtaba Bagheri
    Leibniz Institute of Molecular Pharmacology FMP, Berlin, Germany
    Antimicrob Agents Chemother 55:788-97. 2011
    ..coli activity of the cyclic peptides...
  12. ncbi request reprint Synthesis of a chiral amino acid with bicyclo[1.1.1]pentane moiety and its incorporation into linear and cyclic antimicrobial peptides
    Stephan Pritz
    Leibniz Institute of Molecular Pharmacology, Robert Rossle Strasse 10, 13125, Berlin, Germany
    Org Biomol Chem 5:1789-94. 2007
    ....
  13. doi request reprint Apolipoprotein E peptide-modified colloidal carriers: the design determines the mechanism of uptake in vascular endothelial cells
    Eik Leupold
    Leibniz Institute of Molecular Pharmacology FMP, Robert Roessle Strasse 10, 13125 Berlin, Germany
    Biochim Biophys Acta 1788:442-9. 2009
    ..In conclusion, the specific clathrin-mediated uptake mode of P2A2 micelles might provide the basis for a blood brain barrier-specific targeting...
  14. pmc High-affinity AKAP7delta-protein kinase A interaction yields novel protein kinase A-anchoring disruptor peptides
    Christian Hundsrucker
    Leibniz Institut fur Molekulare Pharmakologie, Campus Berlin Buch, Robert Rossle Str 10, 13125 Berlin, Germany
    Biochem J 396:297-306. 2006
    ....
  15. doi request reprint Insight into the role of HSPG in the cellular uptake of apolipoprotein E-derived peptide micelles and liposomes
    Eik Leupold
    Leibniz Institute of Molecular Pharmacology FMP, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Biochim Biophys Acta 1778:2781-9. 2008
    ..Both routes differ with respect to transport efficiency. The results provide a prospective basis to optimize liposomes and micelles as drug delivery systems...
  16. ncbi request reprint Continuous measurement of rapid transbilayer movement of a pyrene-labeled phospholipid analogue
    P Muller
    Humboldt Universitat zu Berlin, Mathematisch Naturwissenschaftliche Fakultät I, Institut für Biologie Biophysik, Invalidenstrasse 43, D 10115, Berlin, Germany
    Chem Phys Lipids 106:89-99. 2000
    ..An approach is presented which allows estimating the transbilayer distribution of pyPC from the measured ratio I(E)/I(M)...
  17. doi request reprint Nanoparticles for skin penetration enhancement--a comparison of a dendritic core-multishell-nanotransporter and solid lipid nanoparticles
    Sarah Küchler
    Institut fur Pharmazie, Freie Universitat Berlin, Berlin, Germany
    Eur J Pharm Biopharm 71:243-50. 2009
    ..8- and 6.3-fold). Viable human keratinocytes showed an internalisation of both nanocarriers. In conclusion, CMS nanotransporters can favour the penetration of a model dye into the skin even more than SLN which may reflect size effects...
  18. ncbi request reprint A simple fluorescence-spectroscopic membrane translocation assay
    Sandro Keller
    Leibniz Institute of Molecular Pharmacology FMP, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Chembiochem 8:546-52. 2007
    ....
  19. ncbi request reprint Structure of the antimicrobial, cationic hexapeptide cyclo(RRWWRF) and its analogues in solution and bound to detergent micelles
    Christian Appelt
    Forschungsinstitut fur Molekulare Pharmakologie, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Chembiochem 6:1654-62. 2005
    ..They exhibit remarkable similarities in backbone conformation and side-chain orientation. The structure of these peptides allows the side-chain properties to be correlated to biological activity...
  20. ncbi request reprint Dipalmitoylation of a cellular uptake-mediating apolipoprotein E-derived peptide as a promising modification for stable anchorage in liposomal drug carriers
    Ines Sauer
    Leibniz Institute of Molecular Pharmacology FMP, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Biochim Biophys Acta 1758:552-61. 2006
    ..The concept of complexation using dipalmitoylated peptides may offer an efficient substitute to covalent vector coupling and a prospective way to optimize the capacity of liposomes as drug delivery systems also for different targets...
  21. ncbi request reprint Modulation of membrane activity of amphipathic, antibacterial peptides by slight modifications of the hydrophobic moment
    T Wieprecht
    Forschungsinstitut fur Molekulare Pharmakologie, Berlin, Germany
    FEBS Lett 417:135-40. 1997
    ..Correlation of permeabilization and binding indicated that the activity increase is predominantly caused by an increased membrane affinity of the peptides due to strengthened hydrophobic interactions...
  22. ncbi request reprint Noncovalent immobilized artificial membrane chromatography, an improved method for describing peptide-lipid bilayer interactions
    E Krause
    Institute of Molecular Pharmacology, Berlin, Germany
    J Chromatogr A 849:125-33. 1999
    ..The results demonstrate that the chromatographic retention behavior of peptides on noncovalent IAM stationary phase shows an excellent correlation with lipid affinities to phospholipid vesicles...
  23. ncbi request reprint A role for a helical connector between two receptor binding sites of a long-chain peptide hormone
    M Beyermann
    Forschungsinstitut fur Molekulare Pharmakologie, Alfred Kowalke Str 4, D 10315 Berlin, Germany
    J Biol Chem 275:5702-9. 2000
    ..6-50 nM), however, suggest the relative orientation between the two binding sites rather than the maintenance of a distinct distance between them to be essential for a high potency...
  24. doi request reprint The role of apolipoprotein E in uptake of atovaquone into the brain in murine acute and reactivated toxoplasmosis
    Hend M Shubar
    Institute of Microbiology and Hygiene, Charite University Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany
    J Drug Target 17:257-67. 2009
    ..ApoE- and apoE-derived peptides do not induce the uptake of ANSs into the brain. Alternative mechanisms seem to be in operation, thereby mediating the passage of atovaquone across the BBB...
  25. ncbi request reprint Membrane-mimetic nanocarriers formed by a dipalmitoylated cell-penetrating peptide
    Sandro Keller
    Research Institute of Molecular Pharmacology FMP, Berlin, Germany
    Angew Chem Int Ed Engl 44:5252-5. 2005
  26. ncbi request reprint An apolipoprotein E-derived peptide mediates uptake of sterically stabilized liposomes into brain capillary endothelial cells
    Ines Sauer
    Research Institute of Molecular Pharmacology, Robert Roessle Strasse 10, D 13125 Berlin, Germany
    Biochemistry 44:2021-9. 2005
    ..The results suggested that HSPG played a major role in the apoE-peptide-mediated uptake of liposomes into endothelial cells of brain microvessels...
  27. ncbi request reprint Hydrophobicity, hydrophobic moment and angle subtended by charged residues modulate antibacterial and haemolytic activity of amphipathic helical peptides
    M Dathe
    Institute of Molecular Pharmacology, Berlin, Germany
    FEBS Lett 403:208-12. 1997
    ..However, the increase of H, mu and phi substantially enhances haemolytic and Gram-positive antibacterial activity and is related to a reduction of peptide specificity for Gram-negative bacteria...
  28. ncbi request reprint Recognition of alpha-helical peptide structures using high-performance liquid chromatographic retention data for D-amino acid analogues: influence of peptide amphipathicity and of stationary phase hydrophobicity
    S Rothemund
    , Berlin, Germany
    J Chromatogr A 689:219-26. 1995
    ..The results demonstrate that reversed-phase HPLC data for peptide analogues provide an indication event of a non-amphipathic helical structure in peptides...
  29. pmc A critical reassessment of penetratin translocation across lipid membranes
    Elsa Bárány-Wallje
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, Stockholm, Sweden
    Biophys J 89:2513-21. 2005
    ....
  30. pmc Infrared reflection absorption spectroscopy of amphipathic model peptides at the air/water interface
    Andreas Kerth
    Institut fur Physikalische Chemie, Martin Luther Universitat Halle Wittenberg, Halle, Germany
    Biophys J 86:3750-8. 2004
    ..Using the optimal matching hydrophobicity scale or the whole-residue hydrophobicity scale the beta-sheet even has the larger hydrophobic moment...
  31. ncbi request reprint Cupiennin 1d*: the cytolytic activity depends on the hydrophobic N-terminus and is modulated by the polar C-terminus
    Lucia Kuhn-Nentwig
    Zoological Institute, University of Bern, Baltzerstrasse 6, CH 3012, Bern, Switzerland
    FEBS Lett 527:193-8. 2002
    ..The polar C-terminus seems to modulate peptide accumulation at negatively charged cell surfaces via electrostatic interactions and has no important effect on the peptides' amphipathic secondary structure...
  32. doi request reprint Biophysics meets membrane-active peptides
    Miguel A R B Castanho
    J Pept Sci 14:365-7. 2008
  33. ncbi request reprint Cupiennin 1, a new family of highly basic antimicrobial peptides in the venom of the spider Cupiennius salei (Ctenidae)
    Lucia Kuhn-Nentwig
    Zoological Institute, University of Bern, Baltzerstrasse 6, CH 3012 Bern, Switzerland
    J Biol Chem 277:11208-16. 2002
    ..The immediate biological effects and the structural properties of the isolated cupiennins indicate a membrane-destroying mode of action on prokaryotic as well as eukaryotic cells...