Arnulf Kletzin

Summary

Affiliation: Darmstadt University of Technology
Country: Germany

Publications

  1. pmc Molecular analysis of pDL10 from Acidianus ambivalens reveals a family of related plasmids from extremely thermophilic and acidophilic archaea
    A Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    Genetics 152:1307-14. 1999
  2. ncbi request reprint A Rieske ferredoxin typifying a subtype within Rieske proteins: spectroscopic, biochemical and stability studies
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEBS Lett 579:1020-6. 2005
  3. ncbi request reprint Dissimilatory oxidation and reduction of elemental sulfur in thermophilic archaea
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    J Bioenerg Biomembr 36:77-91. 2004
  4. pmc The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochem J 381:137-46. 2004
  5. ncbi request reprint Coupling of the pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound thiosulphate:quinone oxidoreductase
    Fabian H Müller
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    Mol Microbiol 53:1147-60. 2004
  6. ncbi request reprint X-ray Structure of a self-compartmentalizing sulfur cycle metalloenzyme
    Tim Urich
    Darmstadt University of Technology, Institute of Microbiology and Genetics, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Science 311:996-1000. 2006
  7. ncbi request reprint The sulfur oxygenase reductase from Acidianus ambivalens is an icosatetramer as shown by crystallization and Patterson analysis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochim Biophys Acta 1747:267-70. 2005
  8. doi request reprint Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universitat Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Mol Microbiol 73:58-72. 2009
  9. pmc An Extracellular Tetrathionate Hydrolase from the Thermoacidophilic Archaeon Acidianus Ambivalens with an Activity Optimum at pH 1
    Jonas Protze
    Institute of Microbiology and Genetics, Technische Universität Darmstadt Darmstadt, Germany
    Front Microbiol 2:68. 2011
  10. ncbi request reprint Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEMS Microbiol Lett 248:171-6. 2005

Collaborators

  • Claudio M Gomes
  • Carlos Frazao
  • Reinhard Rachel
  • Christa Schleper
  • Stephan C Schuster
  • C W Sensen
  • Sonja Verena Albers
  • M M Pereira
  • Tim Urich
  • Andreas Veith
  • Alexander H Treusch
  • Jonas Protze
  • Friedrich Lottspeich
  • Tiago M Bandeiras
  • Reinhard Mentele
  • Karin Lauber
  • Miguel Teixeira
  • Hugo M Botelho
  • Achim Quaiser
  • Kerstin Seyfarth
  • Ana P Batista
  • Vesna Prosinecki
  • Fabian H Müller
  • Hans Peter Klenk
  • Guido Meurer
  • Simone Laska
  • Torsten Ochsenreiter
  • Florian Kindinger
  • Fabian Müller
  • Bastian Naß
  • Andreas Klingl
  • Behnam Zolghadr
  • Simona Francese
  • Gloriano Moneti
  • Guido Mastrobuoni
  • Christian Bauer
  • Ricardo Coelho
  • Muriel Reuff
  • Sven Leininger
  • Anja Kroke
  • Peter Zimmermann
  • Guenter Raddatz
  • Sónia S Leal
  • Corinna Scholz
  • Till Albrecht
  • Carlos Salgueiro
  • Jürgen Eck

Detail Information

Publications20

  1. pmc Molecular analysis of pDL10 from Acidianus ambivalens reveals a family of related plasmids from extremely thermophilic and acidophilic archaea
    A Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    Genetics 152:1307-14. 1999
    ..solfataricus. Single-stranded DNA of both pDL10 strands was detected with a mung bean nuclease protection assay using PCR detection of protected fragments, giving additional evidence for an RC mechanism of replication...
  2. ncbi request reprint A Rieske ferredoxin typifying a subtype within Rieske proteins: spectroscopic, biochemical and stability studies
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEBS Lett 579:1020-6. 2005
    ..This difference is rationalized in terms of the protein structure environment, as discrete amino acid substitutions in key positions around the metal centre account for the higher potential...
  3. ncbi request reprint Dissimilatory oxidation and reduction of elemental sulfur in thermophilic archaea
    Arnulf Kletzin
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    J Bioenerg Biomembr 36:77-91. 2004
    ..The current knowledge on the composition and properties of the aerobic and anaerobic pathways of dissimilatory elemental sulfur metabolism in thermophilic archaea is summarized in this contribution...
  4. pmc The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochem J 381:137-46. 2004
    ....
  5. ncbi request reprint Coupling of the pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound thiosulphate:quinone oxidoreductase
    Fabian H Müller
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    Mol Microbiol 53:1147-60. 2004
    ..The larger subunit appeared to be glycosylated and was identical to DoxA, and the smaller was identical to DoxD. Both subunits had been described previously as a part of the terminal quinol:oxygen oxidoreductase complex (cytochrome aa3)...
  6. ncbi request reprint X-ray Structure of a self-compartmentalizing sulfur cycle metalloenzyme
    Tim Urich
    Darmstadt University of Technology, Institute of Microbiology and Genetics, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Science 311:996-1000. 2006
    ..The iron is likely the site of both sulfur oxidation and sulfur reduction...
  7. ncbi request reprint The sulfur oxygenase reductase from Acidianus ambivalens is an icosatetramer as shown by crystallization and Patterson analysis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Biochim Biophys Acta 1747:267-70. 2005
    ....
  8. doi request reprint Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universitat Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Mol Microbiol 73:58-72. 2009
    ..We propose that the mushroom-shaped 'unit cells' of the Sulfolobales' S-layers consist of three SlaBs anchoring the complex in the membrane and six SlaAs forming the detergent-resistant outer sacculus...
  9. pmc An Extracellular Tetrathionate Hydrolase from the Thermoacidophilic Archaeon Acidianus Ambivalens with an Activity Optimum at pH 1
    Jonas Protze
    Institute of Microbiology and Genetics, Technische Universität Darmstadt Darmstadt, Germany
    Front Microbiol 2:68. 2011
    ..The liberation of TTH by pH shock from otherwise intact cells strongly supports the pseudo-periplasm hypothesis of the S-layer of Archaea...
  10. ncbi request reprint Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis
    Tim Urich
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    FEMS Microbiol Lett 248:171-6. 2005
    ..It was concluded that the Fe site with H86, H90 and E114 as ligands and C31 constitute the core active site whereas C101 and C104 optimize reaction conditions...
  11. pmc The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universitat Darmstadt, Darmstadt, Germany
    J Bacteriol 194:677-85. 2012
    ..We concluded that the thermostability was the result of a rigid protein core together with the stabilizing effect of the 24-subunit hollow sphere...
  12. ncbi request reprint Characterization of large-insert DNA libraries from soil for environmental genomic studies of Archaea
    Alexander H Treusch
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Environ Microbiol 6:970-80. 2004
    ..We thereby extend the genomic information of uncultivated crenarchaeota from soil and offer hints to specific metabolic traits present in this group...
  13. pmc Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of acidianus ambivalens
    Andreas Veith
    Institute of Microbiology and Genetics, Technische Universität Darmstadt Darmstadt, Germany
    Front Microbiol 2:37. 2011
    ..Hg(2+) and IAA block cysteines in the active site pocket, while Zn(2+) interferes over a distance, possibly by restriction of protein flexibility or substrate access or product exit...
  14. ncbi request reprint Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens
    Simone Laska
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Microbiology 149:2357-71. 2003
    ..A model of sulfur respiration in Acidianus developed from the biochemical results and the data of the amino acid sequence comparisons is discussed...
  15. ncbi request reprint First insight into the genome of an uncultivated crenarchaeote from soil
    Achim Quaiser
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Environ Microbiol 4:603-11. 2002
    ..The protein encoding genes gave the first insights into the physiological potential of these organisms and can serve as a basis for future genomic and functional genomic studies...
  16. ncbi request reprint An archaeal b-type cytochrome containing a nonfunctional carbonic anhydrase-like domain
    Claudio M Gomes
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, 2780 156 Oeiras, Portugal
    J Biol Inorg Chem 7:483-9. 2002
    ..The role of zinc as a key element for the intrinsic thermal stability of these proteins is discussed...
  17. ncbi request reprint Novel genes for nitrite reductase and Amo-related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling
    Alexander H Treusch
    University of Bergen, Department of Biology, Jahnebakken 5, N 5020 Bergen, Norway
    Environ Microbiol 7:1985-95. 2005
    ..Taken together, our findings suggest that mesophilic terrestrial and marine crenarchaeota might be capable of ammonia oxidation under aerobic and potentially also under anaerobic conditions...
  18. ncbi request reprint A proteomic approach toward the selection of proteins with enhanced intrinsic conformational stability
    Vesna Prosinecki
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Proteome Res 5:2720-6. 2006
    ..This work thus illustrates an experimental approach aimed at mining a proteome for hyperstable proteins, a valuable tool for target selection in protein stability and structural studies...
  19. doi request reprint The dihydrolipoamide dehydrogenase from the crenarchaeon Acidianus ambivalens
    Ana P Batista
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    FEMS Microbiol Lett 281:147-54. 2008
    ..Although DLDH is generally part of the alpha-ketoacid dehydrogenase complexes in Bacteria and Eukarya, none of these complexes has yet been isolated from Sulfolobales. The metabolic role of DLDH in these organisms is discussed...
  20. ncbi request reprint Acidianus ambivalens type-II NADH dehydrogenase: genetic characterisation and identification of the flavin moiety as FMN
    Tiago M Bandeiras
    Instituto de Tecnologia Qui mica e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Oeiras, Portugal
    FEBS Lett 531:273-7. 2002
    ..Further, it is suggested that membrane anchoring can be achieved via amphipatic helices...