Research Topics
| Sonja A DamesSummaryCountry: Germany Publications
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Detail Information
Publications
Structural basis for the association of the redox-sensitive target of rapamycin FATC domain with membrane-mimetic micellesSonja A Dames
Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
J Biol Chem 285:7766-75. 2010....
Structure, dynamics, lipid binding, and physiological relevance of the putative GTPase-binding domain of Dictyostelium formin CSonja A Dames
Department of Chemistry, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
J Biol Chem 286:36907-20. 2011..Our cellular localization studies show that both the GBD and the FH3 domain are required for ForC targeting to cell-cell contacts and early phagocytic cups and macropinosomes...
1H, 15N, and 13C assignments of the N-terminal activation domain of Dictyostelium discoideum Formin CSonja A Dames
Department of Chemistry, Biomolecular NMR Spectroscopy, Technische Universitat Munchen, Garching, Germany
Biomol NMR Assign 5:47-9. 2011..Chemical shifts have been deposited at the BioMagResBank under the BMRB accession number 17,029. The N-terminal region of the 131 kDa ForC protein is supposed to form a GTPase-binding domain required for activation of the formin...
Structure of the Cyclin T binding domain of Hexim1 and molecular basis for its recognition of P-TEFbSonja A Dames
Department of Structural Biology, Biozentrum Basel, University of Basel, 4003 Basel, Switzerland
Proc Natl Acad Sci U S A 104:14312-7. 2007..Thus, our studies provide structural insights how Hexim1 recognizes the Cyclin T1 subunit of P-TEFb, which is a key step toward the regulation of transcription elongation...
NMR assignment of the Cyclin T-binding domain of human Hexim1Sonja A Dames
Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstr. 70, Basel, 4056, Switzerland
J Biomol NMR 36:39. 2006
The solution structure of the FATC domain of the protein kinase target of rapamycin suggests a role for redox-dependent structural and cellular stabilitySonja A Dames
Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
J Biol Chem 280:20558-64. 2005..Because the amount of TOR mRNA is not changed, the redox state of the FATC disulfide bond is probably influencing the degradation of TOR...
Insights into the low adhesive capacity of human T-cadherin from the NMR structure of Its N-terminal extracellular domainSonja A Dames
Department of Structural Biology, University of Basel, Klingelbergstr 70, 4056 Basel, Switzerland
J Biol Chem 283:23485-95. 2008..The apparent low adhesiveness of T-cadherin suggests that it is likely to be involved in reversible and dynamic cellular adhesion-deadhesion processes, which are consistent with its role in cell growth and migration...
A fast and simple method for probing the interaction of peptides and proteins with lipids and membrane-mimetics using GB1 fusion proteins and NMR spectroscopyLisa A M Sommer
Department of Chemistry, Biomolecular NMR Spectroscopy, Technische Universitat Munchen, Munich, Germany
Protein Sci 21:1566-70. 2012..To demonstrate the usefulness of the approach, we show NMR binding data for the wild type protein and a leucine to alanine mutant...
The FKBP-rapamycin binding domain of human TOR undergoes strong conformational changes in the presence of membrane mimetics with and without the regulator phosphatidic acidDiana C Rodriguez Camargo
Biomolecular NMR Spectroscopy, Department of Chemistry, Technische Universitat Munchen, Munich, Germany
Biochemistry 51:4909-21. 2012..Consistent with overlapping binding surfaces for different lipids and the FKBP12-rapamycin complex, binding of the inhibitor complex protects the FRB domain from interactions with membrane mimetics at lower lipid concentrations...
A fast and simple method to prepare the FKBP-rapamycin binding domain of human target of rapamycin for NMR binding assaysSonja A Dames
Biozentrum Basel, Department of Structural Biology, University of Basel, Klingelbergstr 70, 4056 Basel, Switzerland
Protein Expr Purif 59:31-7. 2008..The procedure is very simple and can easily be scaled up to prepare large amounts of functional protein for high-throughput cancer drug screening assays by NMR and other techniques...
