Schuller J, Falk S, Fromm L, Hurt E, Conti E. Structure of the nuclear exosome captured on a maturing preribosome. Science. 2018;360:219-222 pubmed publisher
..8S rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60S substrate to process rRNA during ribosome maturation. ..
Benda C, Ebert J, Scheltema R, Schiller H, BaumgÃ¤rtner M, Bonneau F, et al
. Structural model of a CRISPR RNA-silencing complex reveals the RNA-target cleavage activity in Cmr4. Mol Cell. 2014;56:43-54 pubmed publisher
..The inner surface of the superhelix exposes conserved residues of Cmr4 that we show are required for target-cleavage activity. The structural and biochemical data thus identify Cmr4 as the conserved endoribonuclease of the Cmr complex. ..
Prabu J, MÃ¼ller M, Thomae A, SchÃ¼ssler S, Bonneau F, Becker P, et al
. Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. Mol Cell. 2015;60:487-99 pubmed publisher
..The structure visualizes a transition-state mimic of the reaction and suggests how eukaryotic DEAH/RHA helicases couple ATP hydrolysis to RNA translocation. ..
Chakrabarti S, Bonneau F, Schüssler S, Eppinger E, Conti E. Phospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5-SMG7 and SMG6. Nucleic Acids Res. 2014;42:9447-60 pubmed publisher
..Our study demonstrates that SMG5-SMG7 and SMG6 exhibit different and non-overlapping modes of UPF1 recognition, thus pointing at distinguished roles in integrating the complex NMD interaction network. ..
Falk S, Weir J, Hentschel J, Reichelt P, Bonneau F, Conti E. The molecular architecture of the TRAMP complex reveals the organization and interplay of its two catalytic activities. Mol Cell. 2014;55:856-867 pubmed publisher
..The results reveal conceptual similarities between the two major regulators of the exosome, the nuclear TRAMP and cytoplasmic Ski complexes. ..
Bhaskar V, Basquin J, Conti E. Architecture of the ubiquitylation module of the yeast Ccr4-Not complex. Structure. 2015;23:921-928 pubmed publisher
..The structural and biochemical data show how Not1 can incorporate both the ubiquitylation module and the Not2-Not3/5 module concomitantly in the Ccr4-Not complex. ..
Kowalinski E, Schuller A, Green R, Conti E. Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases. Structure. 2015;23:1336-43 pubmed publisher
Nakel K, Bonneau F, Eckmann C, Conti E. Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3. Proc Natl Acad Sci U S A. 2015;112:8614-9 pubmed publisher
..GLD-2 thus appears to have evolved specialized nucleotidyl-transferase properties that match the 3' end features of dormant cytoplasmic mRNAs. ..
Ozgur S, Basquin J, Kamenska A, Filipowicz W, Standart N, Conti E. Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex. Cell Rep. 2015;13:703-711 pubmed publisher
..The results underscore the presence of a complex network of simultaneous and/or mutually exclusive interactions in DDX6-mediated repression. ..