Research Topics
Species | Marcus BantscheffSummaryAffiliation: Cellzome AG Country: Germany Publications
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Detail Information
Publications
Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexesMarcus Bantscheff
Cellzome AG, Heidelberg, Germany
Nat Biotechnol 29:255-65. 2011..Our approach enables the discovery of novel targets and inhibitors and suggests that the selectivity of HDAC inhibitors should be evaluated in the context of HDAC complexes and not purified catalytic subunits...
Quantitative mass spectrometry in proteomics: a critical reviewMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, 69254, Heidelberg, Germany
Anal Bioanal Chem 389:1017-31. 2007..In this review, we critically examine the more commonly used quantitative mass spectrometry methods for their individual merits and discuss challenges in arriving at meaningful interpretations of quantitative proteomic data...- Quantitative mass spectrometry in proteomics: critical review update from 2007 to the presentMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany
Anal Bioanal Chem 404:939-65. 2012..In this review, we focus on the major technical and conceptual developments since 2007 and illustrate these by important recent applications... 
Hsp90 inhibition differentially destabilises MAP kinase and TGF-beta signalling components in cancer cells revealed by kinase-targeted chemoproteomicsArmin Haupt
Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
BMC Cancer 12:38. 2012..However, its inhibition in healthy cells could also lead to severe side effects. This is the first comprehensive analysis of the response to Hsp90 inhibition at the kinome level...- Chemoproteomic approaches to drug target identification and drug profilingMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Bioorg Med Chem 20:1973-8. 2012.... - Robust and sensitive iTRAQ quantification on an LTQ Orbitrap mass spectrometerMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Mol Cell Proteomics 7:1702-13. 2008..This article gives practical guidance for the implementation of PQD, discusses its merits, and for the first time, compares its performance to higher energy collision-induced dissociation... - Confident phosphorylation site localization using the Mascot Delta ScoreMikhail M Savitski
Cellzome AG, Heidelberg, Germany
Mol Cell Proteomics 10:M110.003830. 2011.... - Determination of kinase inhibitor potencies in cell extracts by competition binding assays and isobaric mass tagsCarsten Hopf
Cellzome AG, Heidelberg, Germany
Methods Mol Biol 803:141-55. 2012..The method employs quantitative mass spectrometry using isobaric mass tags which enables determination of potency for a large number of target proteins in a single analysis... - High-resolution enabled TMT 8-plexingThilo Werner
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Anal Chem 84:7188-94. 2012..The increased multiplexing rate enabled determination of inhibitor potencies in chemoproteomic kinase assays covering a wider range of compound concentrations in a single experiment, compared to conventional 6-plex TMT-based assays... - Delayed fragmentation and optimized isolation width settings for improvement of protein identification and accuracy of isobaric mass tag quantification on Orbitrap-type mass spectrometersMikhail M Savitski
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Anal Chem 83:8959-67. 2011..When delayed peptide fragmentation and narrow isolation width settings were both applied, cofragmentation-induced ratio compression could be reduced by 32% on a log2 scale under otherwise identical conditions... - Quantitative chemical proteomics reveals mechanisms of action of clinical ABL kinase inhibitorsMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Biotechnol 25:1035-44. 2007..The data suggest that our approach is a valuable tool for drug discovery... - Targeted data acquisition for improved reproducibility and robustness of proteomic mass spectrometry assaysMikhail M Savitski
Department of Analytical Sciences, Cellzome AG, Heidelberg, Germany
J Am Soc Mass Spectrom 21:1668-79. 2010..We have observed excellent reproducibility between different instruments, underlining the robustness of the approach... - Mass spectrometry approaches to monitor protein-drug interactionsNico Zinn
Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Methods 57:430-40. 2012..This review provides an overview of current methods for the characterization of drug-target interactions by mass spectrometry and describes a protocol for chemoproteomic target binding studies using immobilized bioactive molecules... - Affinity purification of proteins binding to kinase inhibitors immobilized on self-assembling monolayersMarcus Bantscheff
Cellzome AG, Heidelberg, Germany
Methods Mol Biol 795:149-60. 2012..The method can be applied to assess the selectivity of a given kinase inhibitor and thus to guide its preclinical or clinical development... - Evaluation of data analysis strategies for improved mass spectrometry-based phosphoproteomicsMikhail M Savitski
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Anal Chem 82:9843-9. 2010.... - H-score, a mass accuracy driven rescoring approach for improved peptide identification in modification rich samplesMikhail M Savitski
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
J Proteome Res 9:5511-6. 2010..When applied on a kinase-enriched sample containing only a few modifications, a smaller but still significant gain of 5% was observed... - A selective inhibitor reveals PI3Kγ dependence of T(H)17 cell differentiationGiovanna Bergamini
Cellzome AG, Heidelberg, Germany
Nat Chem Biol 8:576-82. 2012.... - Revealing promiscuous drug-target interactions by chemical proteomicsMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Drug Discov Today 14:1021-9. 2009..This information can contribute toward optimization of candidate drug molecules and reduction of side effects. In this review, we describe recent advances in chemical proteomics and outline potential applications in drug discovery... - Pathway proteomics and chemical proteomics team up in drug discoveryCarsten Hopf
Cellzome AG, Department of Discovery Research, Heidelberg, Germany
Neurodegener Dis 4:270-80. 2007..The present review discusses some of the current experimental strategies, with a focus on applications in neurobiology... - Chemical and pathway proteomics: powerful tools for oncology drug discovery and personalized health careUlrich Kruse
Deptartment of Discovery Technology, Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Mol Cell Proteomics 7:1887-901. 2008..Moreover we highlight the emerging utility of pathway and chemical proteomics techniques for translational research in patient tissue... - Femtomol sensitivity post-digest (18)O labeling for relative quantification of differential protein complex compositionMarcus Bantscheff
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Rapid Commun Mass Spectrom 18:869-76. 2004..This paper presents quantification data for a set of standard proteins, as well as a study of differential complex formation around the NFkappaB transcription factor p65 following stimulation with TNF-alpha... - Mass spectrometry-based chemoproteomic approachesMarcus Bantscheff
Cellzome AG, Heidelberg, Germany
Methods Mol Biol 803:3-13. 2012..For this purpose, a large arsenal of approaches has emerged in recent years, many of which employing quantitative mass spectrometry. This review briefly summarizes major experiment types employed in current chemical proteomics research... - Chemoproteomics-Based Design of Potent LRRK2-Selective Lead Compounds That Attenuate Parkinson's Disease-Related Toxicity in Human NeuronsNigel Ramsden
Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
ACS Chem Biol 6:1021-8. 2011..These precise chemical probes further validate this emerging therapeutic strategy. They will enable more detailed studies of LRRK2-dependent signaling and pathogenesis and accelerate drug discovery... - Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritisAndrea Sinz
Proteome Center Rostock, Medical Faculty, University of Rostock, Germany
Electrophoresis 23:3445-56. 2002.... - Proteome analysis of diseased joints from mice suffering from collagen-induced arthritisPeter Lorenz
Institute of Immunology, Medical Faculty, University of Rostock, Rostock, Germany
Clin Chem Lab Med 41:1622-32. 2003..This study provides us with the framework for more detailed approaches to understanding the complex disease arthritis that go beyond global proteomics... - Human Proteinpedia enables sharing of human protein dataSuresh Mathivanan
Nat Biotechnol 26:164-7. 2008 - An improved two-step calibration method for matrix-assisted laser desorption/ionization time-of-flight mass spectra for proteomicsMarcus Bantscheff
Rapid Commun Mass Spectrom 16:1892-5. 2002