Research Topics
| U CassensSummaryCountry: Germany Publications
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Detail Information
Publications
Factors affecting the efficacy of peripheral blood progenitor cells collections by large-volume leukaphereses with standardized processing volumesUwe Cassens
Institute of Transfusion Medicine, Department of Medical Informatics and Biomathematics, University Hospital Munster, Germany
Transfusion 44:1593-602. 2004..Peripheral blood progenitor cell (PBPC) collections should be safe and efficient. Therefore, the influence and risk factors in large-volume leukaphereses (LVL) with standardized blood volumes was investigated...- Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwideUwe Cassens
Institute of Transfusion Medicine, University Hospital Munster, Germany
Antivir Ther 9:395-405. 2004..2%. The low-cost device and the simplified no-lyse, no-wash test procedure reduces the costs per determination and facilitates the use of flow cytometry in developing countries... - Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contaminationUwe Cassens
Department of Transfusion Medicine Transplantation Immunology, University Hospital Munster, Germany
Transfusion 42:10-7. 2002..Therefore, principles of Good Manufacturing Practice (GMP) are applicable for processing of PBPC components to reduce potential risks of contamination... - A novel true volumetric method for the determination of residual leucocytes in blood componentsU Cassens
Institute for Transfusion Medicine Department for Transplantation Immunology, University Hospital Munster, Germany
Vox Sang 82:198-206. 2002..Accurate determination of residual leucocytes [white blood cells (WBC)] in blood components is of high clinical importance. To date, several labour-intensive, time-consuming or expensive techniques have been used for this purpose... - Circulatory arrest during PBPC apheresis in an unrelated donorUwe Cassens
Institute of Transfusion Medicine Department of Transplantation Immunology, University Hospital Muenster, Muenster, Germany
Transfusion 43:736-41. 2003..Nowadays, the collection of PBPCs by apheresis from healthy donors is a routine method. The mobilization with rHu G-CSF and the apheresis procedures are usually well tolerated without severe side effects... - Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)U Cassens
Department of Transfusion Medicine/Transplantation Immunology, University Hospital Muenster, Muenster, Germany
Bone Marrow Transplant 28:13-20. 2001..In summary, a recruitment phenomenon, defined as a time-dependent and LVL-induced increase of PBPC, could not be demonstrated in any of the diseases investigated... 
The isotype of autoantibodies influences the phagocytosis of antibody-coated platelets in autoimmune thrombocytopenic purpuraM Hoemberg
Institute of Transfusion Medicine and Transplantation Immunology, University Hospital Muenster, Germany
Scand J Immunol 74:489-95. 2011..Therefore, the AITP-mediating autoantibody isotype should be considered more carefully in pathophysiologic models and furthermore in diagnostic, therapeutic and prognostic approaches in AITP...- Quantification of PCR products using microparticles and flow cytometryN Wedemeyer
Institut fur Strahlenbiologie, Westfalische Wilhelms Universitat Munster, Robert Koch Str 43, 48149 Munster, Germany
Ann Biol Clin (Paris) 62:65-71. 2004..We conclude that the analysis of PCR products using microparticles and flow cytometry fulfils the criteria of clinical routine diagnostics regarding (i) sensitivity, (ii) specificity, (iii) reproducibility and (iv) automatibility... - Strategies to reduce perioperative blood loss related to non-surgical bleedingA Menzebach
University Hospital Muenster, Department of Anaesthesiology and Intensive Care, Muenster, Germany
Eur J Anaesthesiol 20:764-70. 2003..If this can positively be excluded and if all of measures described fail to reduce or stop the bleeding, further treatment of such uncontrolled bleeding remains symptomatic... - Polymorphisms in the non-coding region of the human mitochondrial genome in unrelated plateletapheresis donorsH S Garritsen
Department of Transfusion Medicine and Transplantation Immunology, Tissue Typing Laboratory, University Hospital Munster, Domagkstr 11, 48149 Germany
Br J Haematol 112:995-1003. 2001.... - Automated serological compatibility testing using a solid-phase test and standard laboratory equipmentN Ostendorf
Department of Transfusion Medicine and Transplantation Immunology, Westfaelische Wilhelms Universitaet, Munster, Germany
Vox Sang 80:225-9. 2001..To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment... - Platelet glycoprotein complex Ia/IIa antibodies cause neonatal alloimmune thrombocytopenia but do not inhibit megakaryopoiesis and platelet recovery after allogeneic cord blood stem cell transplantationU Cassens
Department of Transfusion Medicine/Transplantation Immunology, University Hospital Muenster, Muenster, Germany
Bone Marrow Transplant 28:803-6. 2001..This case demonstrates a successful CB transplantation from a donor suffering from NAIT... - The impact of erythrocyte lysing procedures on the recovery of hematopoietic progenitor cells in flow cytometric analysisBurkhard Greve
Department of Radiobiology, University Hospital Munster, Robert Koch Strasse 43, D 48149 Munster, Germany
Stem Cells 24:793-9. 2006..As demonstrated by the high accuracy of the 1-PF technique, all erythrocyte lysing procedures caused significant cell loss, which led to inconsistent counting of CD34+ cells in nonvolumetric flow cytometric (2-PF) protocols... - Evidence that human autoimmune thrombocytopenia mediated by both immunoglobulin isotypes IgM and IgG is an independent disease entityDorothea Stahl
University of Munster, Institute for Transfusion Medicine, Domagkstrasse 11, D 48149 Munster, Germany
Eur J Haematol 75:318-27. 2005..The role of autoantibody isotype for the pathophysiology of AITP might currently be underestimated, and diagnostic and therapeutic procedures in AITP might profit from considering autoantibody isotype more carefully... - Influence of isotypes of disease-associated autoantibodies on the expression of natural autoantibody repertoires in humansDorothea Stahl
University of Munster, Institute for Transfusion Medicine, Domagkstrasse 11, 48149 Munster, Germany
Immunol Lett 102:50-9. 2006.... - High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analysesBurkhard Greve
Department of Radiobiology, University Hospital Munster, 48149 Munster, Germany
J Hematother Stem Cell Res 12:321-30. 2003.... - Semiquantitative measurement of IgG subclasses and IgM of platelet-specific antibodies in a glycoprotein-specific platelet-antigen capture assayReinhard Kelsch
Department of Transfusion Medicine, University Hospital Munster, Germany
Br J Haematol 117:141-50. 2002..The PAC assay sensitively detects the subclasses of platelet-specific IgG and human leucocyte antigen (HLA)-antibodies independently. It is easy to perform and takes less time than other platelet glycoprotein-specific methods... - Cryopreservation reduces the concentration of detectable bacteria in contaminated peripheral blood progenitor cell productsFrank Kipp
Institute of Medical Microbiology, the Institute of Transfusion Medicine/Transplantation Immunology, and the Department of Biometrics, , Germany
Transfusion 44:1098-103. 2004..Especially in sterility testing of PBPCPs with low bacterial contamination, this phenomenon could lead to false-negative results with severe clinical consequences... - The influence of blood group differences in allogeneic hematopoietic peripheral blood progenitor cell transplantationChristian G Erker
Institute for Transfusion Medicine, University Hospital of Muenster, Muenster, Germany
Transfusion 45:1382-90. 2005..Irregular antibodies also did not show significant influences. CONCLUSION: These findings indicate an influence of blood group differences in PBPCT on survival and must be studied in further detail... - A noninvasive strategy for screening prospective blood donors for anemiaRamin Lotfi
Department of Transfusion Medicine, Eberhard Karls University, Tuebingen, Germany
Transfusion 45:1585-92. 2005..The reliability of capillary hemoglobin (Hb) as an indicator for eligibility to donate blood is discussed controversially. Therefore, a noninvasive alternative with acceptable predictive values was established and evaluated... - The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cellsChristopher Stroh
Institute of Experimental Dermatology, , , Germany
FASEB J 16:1651-3. 2002..The addition of caspase inhibitors may be an important tool for the cryopreservation of living cells and advantageous in cell transplantation, tissue engineering, and other genetic technologies... - Different activation patterns of proinflammatory cytokines in melancholic and non-melancholic major depression are associated with HPA axis activityFlorian Kaestner
Department of Psychiatry, University of Muenster, Albert-Schweitzer-Strasse 11, D-48149 Muenster, Germany
J Affect Disord 87:305-11. 2005..Non-melancholic patients showed signs of inflammation in acute depression with normalization upon remission while the function of the HPA axis was normal...