Holger Christian Scholz

Summary

Affiliation: Bundeswehr Institute of Microbiology
Country: Germany

Publications

  1. pmc Isolation of Brucella microti from soil
    Holger C Scholz
    Emerg Infect Dis 14:1316-7. 2008
  2. pmc Natural Burkholderia mallei infection in Dromedary, Bahrain
    Ulrich Wernery
    Central Veterinary Research Laboratory, Dubai, United Arab Emirates
    Emerg Infect Dis 17:1277-9. 2011
  3. pmc Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing
    Axel Karger
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Molecular Biology, Südufer 10, Greifswald Insel Riems D 17493, Germany
    BMC Microbiol 12:229. 2012
  4. ncbi request reprint Molecular characterisation of Brucella species
    H C Scholz
    Department of Bacteriology and Toxinology, Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Rev Sci Tech 32:149-62. 2013
  5. pmc Use of a Western blot technique for the serodiagnosis of glanders
    Mandy C Elschner
    Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany
    BMC Vet Res 7:4. 2011
  6. pmc Prevalence-dependent use of serological tests for diagnosing glanders in horses
    Lisa D Sprague
    OIE Reference Laboratory for Glanders, Friedrich Loeffler Institute, Naumburger Str, 96a, D 07743 Jena, Germany
    BMC Vet Res 5:32. 2009
  7. pmc Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    BMC Microbiol 10:269. 2010
  8. pmc Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR
    Herbert Tomaso
    Friedrich Loeffler Institut, Institute of Bacterial Infections and Zoonoses, Naumburgerstrasse 96a, 07743 Jena, Germany
    BMC Infect Dis 10:100. 2010
  9. pmc Brucella microti: the genome sequence of an emerging pathogen
    Stephane Audic
    Laboratoire Information Génomique et Structurale, CNRS UPR2589, Aix Marseille University, Institut de Microbiologie de la Méditerranée IM2, IFR 88, Parc Scientifique de Luminy, FR 13288, Marseille Cedex 09, France
    BMC Genomics 10:352. 2009
  10. doi request reprint Brucella microti sp. nov., isolated from the common vole Microtus arvalis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Int J Syst Evol Microbiol 58:375-82. 2008

Collaborators

Detail Information

Publications32

  1. pmc Isolation of Brucella microti from soil
    Holger C Scholz
    Emerg Infect Dis 14:1316-7. 2008
  2. pmc Natural Burkholderia mallei infection in Dromedary, Bahrain
    Ulrich Wernery
    Central Veterinary Research Laboratory, Dubai, United Arab Emirates
    Emerg Infect Dis 17:1277-9. 2011
    ....
  3. pmc Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing
    Axel Karger
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Molecular Biology, Südufer 10, Greifswald Insel Riems D 17493, Germany
    BMC Microbiol 12:229. 2012
    ..The aim of this study was to evaluate the use of MALDI-TOF MS for the rapid and reliable identification and differentiation of B. pseudomallei and B. mallei and to build up a reliable reference database for both organisms...
  4. ncbi request reprint Molecular characterisation of Brucella species
    H C Scholz
    Department of Bacteriology and Toxinology, Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Rev Sci Tech 32:149-62. 2013
    ..These novel approaches should help in further understanding the evolution, host specificity and pathogenicity of the genus Brucella...
  5. pmc Use of a Western blot technique for the serodiagnosis of glanders
    Mandy C Elschner
    Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany
    BMC Vet Res 7:4. 2011
    ..The test was validated investigating a comprehensive set of positive and negative sera obtained from horses and mules from endemic and non endemic areas...
  6. pmc Prevalence-dependent use of serological tests for diagnosing glanders in horses
    Lisa D Sprague
    OIE Reference Laboratory for Glanders, Friedrich Loeffler Institute, Naumburger Str, 96a, D 07743 Jena, Germany
    BMC Vet Res 5:32. 2009
    ..mallei whole cell antigen and a well characterised mouse monoclonal antibody with regard to their specificity and sensitivity for glanders serodiagnosis using CFT, an indirect (i) and a competitive (c) ELISA platform...
  7. pmc Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    BMC Microbiol 10:269. 2010
    ....
  8. pmc Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR
    Herbert Tomaso
    Friedrich Loeffler Institut, Institute of Bacterial Infections and Zoonoses, Naumburgerstrasse 96a, 07743 Jena, Germany
    BMC Infect Dis 10:100. 2010
    ..Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA...
  9. pmc Brucella microti: the genome sequence of an emerging pathogen
    Stephane Audic
    Laboratoire Information Génomique et Structurale, CNRS UPR2589, Aix Marseille University, Institut de Microbiologie de la Méditerranée IM2, IFR 88, Parc Scientifique de Luminy, FR 13288, Marseille Cedex 09, France
    BMC Genomics 10:352. 2009
    ..The atypical phenotype of B. microti prompted us to look for genomic differences compared to other Brucella species and to look for similarities with Ochrobactrum...
  10. doi request reprint Brucella microti sp. nov., isolated from the common vole Microtus arvalis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Int J Syst Evol Microbiol 58:375-82. 2008
    ..nov. with the type strain CCM 4915(T) (=BCCN 07-01(T)=CAPM 6434(T)) is proposed...
  11. doi request reprint Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum-Brucella group by recA and 16S rRNA gene-based comparative sequence analysis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 31:1-16. 2008
    ..In summary, Ochrobactrum is a highly diverse genus comprising several novel species. We recommend recA- in addition to rrs gene-analysis for correct species allocation and subtyping of novel Ochrobactrum isolates...
  12. doi request reprint Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Munich, Germany
    Vector Borne Zoonotic Dis 9:153-6. 2009
    ..Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles...
  13. doi request reprint Brucella inopinata sp. nov., isolated from a breast implant infection
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Int J Syst Evol Microbiol 60:801-8. 2010
    ..Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed...
  14. ncbi request reprint Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    J Med Microbiol 57:64-71. 2008
    ..The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp...
  15. ncbi request reprint Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    FEMS Microbiol Lett 244:347-52. 2005
    ..In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level...
  16. ncbi request reprint Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Munich, Germany
    FEMS Microbiol Lett 257:7-16. 2006
    ..In summary, recA-sequence analysis provides a new reliable molecular subtyping tool to study the phylogeny of the Ochrobactrum taxon at both the inter- and intraspecies level...
  17. ncbi request reprint Detection of Chromobacterium violaceum by multiplex PCR targeting the prgI, spaO, invG, and sipB genes
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 29:45-8. 2006
    ..The detection limit for pure cultures in multiplex PCR analysis was 100CFU. The developed assay significantly improves rapid identification of Chr. violaceum and allows its differentiation from closely related organisms...
  18. ncbi request reprint Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assay
    Holger C Scholz
    Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Diagn Microbiol Infect Dis 54:241-7. 2006
    ..mallei infection. The developed PCR assay can be used as a simple and rapid tool for the specific and sensitive detection of B. mallei in clinical samples...
  19. ncbi request reprint Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex
    Herbert Tomaso
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Diagn Mol Pathol 13:247-53. 2004
    ..mallei/B. pseudomallei complex in positive blood cultures or from suspicious bacterial colonies allowing the early onset of appropriate antibiotic therapy...
  20. ncbi request reprint Failure of a short-term antibiotic therapy for human brucellosis using ciprofloxacin. A study on in vitro susceptibility of Brucella strains
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Chemotherapy 51:352-6. 2005
    ..Human brucellosis is characterized byfocal complications, chronic courses, and therapeutic failures...
  21. ncbi request reprint Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Crit Rev Microbiol 31:191-6. 2005
    ..Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed...
  22. ncbi request reprint Growth characteristics of Bacillus anthracis compared to other Bacillus spp. on the selective nutrient media Anthrax Blood Agar and Cereus Ident Agar
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 29:24-8. 2006
    ..Species which might be misidentified as B. anthracis were B. cereus, B. mycoides, and B. thuringiensis. Particularly, 30% of B. weihenstephanensis strains were misidentified as B. anthracis...
  23. ncbi request reprint Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    J Immunol Methods 309:34-47. 2006
    ..abortus clearly from cross-reacting bacteria. The combination of SELDI-MS analysis with the specificity of antibody binding will improve the identification of Brucella specific immunogenic proteins...
  24. ncbi request reprint Identification and antimicrobial susceptibilities of Ochrobactrum spp
    Bryan Thoma
    Bundeswehr Institute of Microbiology, Munich, Germany
    Int J Med Microbiol 299:209-20. 2009
    ..Our in vitro data suggest that combinations of antimicrobial agents including ciprofloxacin and/or trimethoprim/sulfamethoxazole may be useful for empirical treatment of Ochrobactrum infections...
  25. pmc Brucella endocarditis in prosthetic valves
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Can J Cardiol 22:971-4. 2006
    ..The patient was successfully treated by a medical and surgical approach. Therapeutic strategies in Brucella endocarditis are discussed in light of the current literature...
  26. ncbi request reprint Evaluation of Brucella MLVA typing for human brucellosis
    Sascha Al Dahouk
    Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstr 11, D 80937 Munich, Germany
    J Microbiol Methods 69:137-45. 2007
    ..MLVA can significantly contribute to epidemiological trace-back analysis of Brucella infections and may advance surveillance and control of human brucellosis...
  27. doi request reprint Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plague
    Julia M Riehm
    Bundeswehr Institute of Microbiology, Munich, Germany
    Mol Cell Probes 25:8-12. 2011
    ..In conclusion, the 5'-nuclease assay targeting pla can be recommended as diagnostic tool for establishing a presumptive diagnosis when bubonic plague is clinically suspected...
  28. ncbi request reprint Development of a 5'-nuclease real-time PCR assay targeting fliP for the rapid identification of Burkholderia mallei in clinical samples
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem 52:307-10. 2006
    ..The number of reported outbreaks has increased steadily during the last decade, but diagnosis is hampered by the low bacterial load in infected tissues and excretions...
  29. ncbi request reprint Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes
    Herbert Tomaso
    Federal Armed Forces Institute of Microbiology, Neuherbergstrasse 11, Munich 80937, Germany
    Mol Cell Probes 19:9-20. 2005
    ..In conclusion, we recommend the use of the 16S rDNA and/or fliC real-time PCR assays for the rapid identification of B. mallei and B. pseudomallei in positive blood cultures or from suspicious bacterial colonies...
  30. ncbi request reprint Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensis
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Mol Cell Probes 21:12-6. 2007
    ..This is a major diagnostic improvement, as all other methods for the specific identification of F. tularensis subsp. tularensis are more time consuming...
  31. ncbi request reprint Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem Lab Med 45:1464-70. 2007
    ..We therefore assessed the performance of real-time PCR assays for the identification of members of the genus Brucella to the genus and species level...
  32. doi request reprint Preliminary validation of real-time PCR assays for the identification of Yersinia pestis
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem Lab Med 46:1239-44. 2008
    ..Therefore, rapid and reliable diagnostic tools are crucial. The objective of this study was to assess the inter-laboratory reproducibility of in-house developed real-time PCR assays for the identification of Y. pestis...