Volkmar Braun

Summary

Country: Germany

Publications

  1. ncbi request reprint Ton-dependent colicins and microcins: modular design and evolution
    Volkmar Braun
    Mikrobiologie and Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28 D 72076, Tubingen, Germany
    Biochimie 84:365-80. 2002
  2. ncbi request reprint Active transport of iron and siderophore antibiotics
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076, Tubingen, Germany
    Curr Opin Microbiol 5:194-201. 2002
  3. ncbi request reprint Gene regulation by transmembrane signaling
    Volkmar Braun
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, Tubingen, 72096, Germany
    Biometals 19:103-13. 2006
  4. ncbi request reprint Gene regulation by transmembrane signaling
    Volkmar Braun
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, Tübingen 72096, Germany
    Biometals 18:507-17. 2005
  5. ncbi request reprint Regulation of the FecI-type ECF sigma factor by transmembrane signalling
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universtat Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany
    Curr Opin Microbiol 6:173-80. 2003
  6. ncbi request reprint Iron transport and signaling in Escherichia coli
    Volkmar Braun
    Microbiology Membranephysiology, University of Tubingen, Auf der Morgenstelle 28, D 72076, Tubingen, Germany
    FEBS Lett 529:78-85. 2002
  7. ncbi request reprint Iron uptake by Escherichia coli
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Front Biosci 8:s1409-21. 2003
  8. ncbi request reprint Bacterial solutions to the iron-supply problem
    V Braun
    Lehrstuhl Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Trends Biochem Sci 24:104-9. 1999
  9. ncbi request reprint Energy transfer between biological membranes
    Volkmar Braun
    Microbiology Membranephysiology, University of Tuebingen, Tuebingen, Germany
    ACS Chem Biol 1:352-4. 2006
  10. pmc Point mutations in transmembrane helices 2 and 3 of ExbB and TolQ affect their activities in Escherichia coli K-12
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 186:4402-6. 2004

Collaborators

Detail Information

Publications90

  1. ncbi request reprint Ton-dependent colicins and microcins: modular design and evolution
    Volkmar Braun
    Mikrobiologie and Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28 D 72076, Tubingen, Germany
    Biochimie 84:365-80. 2002
    ..Three of these microcins are modified in an unknown way and they have in common specificity for catecholate siderophore receptors...
  2. ncbi request reprint Active transport of iron and siderophore antibiotics
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076, Tubingen, Germany
    Curr Opin Microbiol 5:194-201. 2002
    ..In this review, recently published data is discussed to demonstrate the state of understanding of iron transport across the outer membrane and the cytoplasmic membrane...
  3. ncbi request reprint Gene regulation by transmembrane signaling
    Volkmar Braun
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, Tubingen, 72096, Germany
    Biometals 19:103-13. 2006
    ..Regulation of the fec genes represents the paradigm of a growing number of gene regulation systems involving transmembrane signaling across three cellular compartments...
  4. ncbi request reprint Gene regulation by transmembrane signaling
    Volkmar Braun
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, Tübingen 72096, Germany
    Biometals 18:507-17. 2005
    ..Regulation of the fec genes represents the paradigm of a growing number of gene regulation systems involving transmembrane signaling across three cellular compartments...
  5. ncbi request reprint Regulation of the FecI-type ECF sigma factor by transmembrane signalling
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universtat Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany
    Curr Opin Microbiol 6:173-80. 2003
    ..With the recent sequencing of complete microbial genomes, it has become apparent that the FecIRA cascade is now a paradigm for the regulatory control of FecI family sigmas in Gram-negative bacteria...
  6. ncbi request reprint Iron transport and signaling in Escherichia coli
    Volkmar Braun
    Microbiology Membranephysiology, University of Tubingen, Auf der Morgenstelle 28, D 72076, Tubingen, Germany
    FEBS Lett 529:78-85. 2002
    ..Certain transport proteins also function in siderophore-mediated signaling cascades that start at the cell surface and flow to the cytoplasm to initiate transcription of genes encoding proteins for transport and siderophore biosynthesis...
  7. ncbi request reprint Iron uptake by Escherichia coli
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Front Biosci 8:s1409-21. 2003
    ..Regulation of ferric citrate transport genes via a transmembrane device has become the paradigm of the regulation of a variety of systems, including the hypersensitivity response of plants to bacterial infections...
  8. ncbi request reprint Bacterial solutions to the iron-supply problem
    V Braun
    Lehrstuhl Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Trends Biochem Sci 24:104-9. 1999
    ..These mechanisms involve transfer of energy from the cytoplasmic membrane to the outer membrane to drive active transport and might induce transcription of transport genes by transmitting a signal from the cell surface...
  9. ncbi request reprint Energy transfer between biological membranes
    Volkmar Braun
    Microbiology Membranephysiology, University of Tuebingen, Tuebingen, Germany
    ACS Chem Biol 1:352-4. 2006
    ..The very similar structures of the protein complexes define the mode of interactions of TonB with active transport proteins...
  10. pmc Point mutations in transmembrane helices 2 and 3 of ExbB and TolQ affect their activities in Escherichia coli K-12
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 186:4402-6. 2004
    ..E176 and T148 are strictly conserved in ExbB and TolQ proteins, and T181 is almost strictly conserved in ExbB, TolQ, and MotA...
  11. ncbi request reprint Bacterial iron transport related to virulence
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, DE 72076 Tübingen, Germany
    Contrib Microbiol 12:210-33. 2005
  12. ncbi request reprint Energy-coupled outer membrane transport proteins and regulatory proteins
    Volkmar Braun
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, 72076 Tubingen, Germany
    Biometals 20:219-31. 2007
    ....
  13. ncbi request reprint Transmembrane transcriptional control (surface signalling) of the Escherichia coli Fec type
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    FEMS Microbiol Rev 29:673-84. 2005
    ..avium, and Ralstonia solanacearum. The genomes of P. putida, P. aeruginosa, Nitrosomonas europaea, Bacteroides thetaiotaomicron and Caulobacter crescentus predict the existence of many more such transcriptional regulatory devices...
  14. pmc Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12
    Annette Sauter
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tuebingen, Germany
    J Bacteriol 186:5303-10. 2004
    ..Comparison of the properties of the FecA, FhuA, FepA, and BtuB transporters indicates that although they have very similar crystal structures, the details of their functional mechanisms differ...
  15. pmc Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli
    Franziska Endriss
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
    J Bacteriol 186:4818-23. 2004
    ..Presumably, FhuA evolved as a ferrichrome transporter and was subsequently used as a receptor by the phages and colicin M, which selected the same as well as distinct loops as receptor sites...
  16. pmc Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface
    C Harle
    Universitat Tubingen, Germany
    EMBO J 14:1430-8. 1995
    ..FecI serves as a sigma factor which facilitates binding of the RNA polymerase to the fec transport gene promoter upstream of fecA.(ABSTRACT TRUNCATED AT 250 WORDS)..
  17. pmc Docking of the periplasmic FecB binding protein to the FecCD transmembrane proteins in the ferric citrate transport system of Escherichia coli
    Volkmar Braun
    Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstasse 35, 72076 Tubingen, Germany
    J Bacteriol 189:6913-8. 2007
    ..The data are consistent with the proposal that FecB(E93) is contained in the region that binds to FecD and FecB(E222) in the region that binds to FecC...
  18. pmc Functional interaction of region 4 of the extracytoplasmic function sigma factor FecI with the cytoplasmic portion of the FecR transmembrane protein of the Escherichia coli ferric citrate transport system
    Susanne Mahren
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 184:3704-11. 2002
    ..aeruginosa proteins and between a P. aeruginosa FecI homolog and E. coli FecR. The results demonstrate that region 4 of FecI specifically binds FecR and that this binding is necessary for FecI to function as a sigma factor...
  19. ncbi request reprint Occurrence and regulation of the ferric citrate transport system in Escherichia coli B, Klebsiella pneumoniae, Enterobacter aerogenes, and Photorhabdus luminescens
    Susanne Mahren
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, 72076 Tubingen, Germany
    Arch Microbiol 184:175-86. 2005
    ..The evidence indicates that fecIRABCDE genes are acquired by horizontal DNA transfer and can undergo debilitating mutations...
  20. pmc Sites of interaction between the FecA and FecR signal transduction proteins of ferric citrate transport in Escherichia coli K-12
    Sabine Enz
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:3745-52. 2003
    ..The data showed that FecR interacts in the periplasm with FecA to confer ferric citrate-induced transcription of the fec transport genes and identified sites in FecR and FecA that are important for signal transduction...
  21. pmc In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli
    S P Howard
    Mikrobiologie II, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 183:5885-95. 2001
    ....
  22. pmc Mutant analysis of the Escherichia coli FhuA protein reveals sites of FhuA activity
    Franziska Endriss
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:4683-92. 2003
    ..The TonB box region has to be flexible, but its distance from the cork domain can greatly vary. The removal of salt bridges between the cork and the barrel affects the structure but not the function of FhuA...
  23. ncbi request reprint TonB of Escherichia coli activates FhuA through interaction with the beta-barrel
    Helmut Killmann
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    Microbiology 148:3497-509. 2002
    ..coli FhuA protein. The data support the theory that the beta-barrel receives the energy from the cytoplasmic membrane via TonB and responds to the energy input and thus represents the transporting domain of FhuA...
  24. pmc In vivo reconstitution of the FhuA transport protein of Escherichia coli K-12
    Michael Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:5508-18. 2003
    ....
  25. ncbi request reprint Energy-coupled transport and signal transduction through the gram-negative outer membrane via TonB-ExbB-ExbD-dependent receptor proteins
    V Braun
    Mikrobiologie II, Universitat Tubingen, Germany
    FEMS Microbiol Rev 16:295-307. 1995
    ....
  26. pmc Control of the ferric citrate transport system of Escherichia coli: mutations in region 2.1 of the FecI extracytoplasmic-function sigma factor suppress mutations in the FecR transmembrane regulatory protein
    A Stiefel
    Mikrobiologie Membranphysiologie, Universitat Tubingen, 72076 Tubingen, Germany
    J Bacteriol 183:162-70. 2001
    ..The data reveal properties of the FecI ECF factor distinct from those of sigma(70) and further support the novel transcription initiation model in which the cytoplasmic portion of FecR is important for FecI activity...
  27. doi request reprint Import of periplasmic bacteriocins targeting the murein
    Volkmar Braun
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    Biochem Soc Trans 40:1449-55. 2012
    ..Cma kills cells only when the periplasmic FkpA PPIase (peptidylprolyl cis-trans isomerase)/chaperone is present...
  28. pmc Sideromycins: tools and antibiotics
    Volkmar Braun
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Tubingen, Germany
    Biometals 22:3-13. 2009
    ..Albomycin could be a useful antibiotic provided sufficient quantities can be isolated from streptomycetes or synthesized chemically...
  29. pmc Interactions between the outer membrane ferric citrate transporter FecA and TonB: studies of the FecA TonB box
    Monica Ogierman
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:1870-85. 2003
    ..Demonstrated here for the first time is the physical interaction of TonB and FecA, which is enhanced by ferric citrate...
  30. ncbi request reprint A FhuA mutant of Escherichia coli is infected by phage T1-independent of TonB
    Johannes Langenscheid
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tuebingen, Germany
    FEMS Microbiol Lett 234:133-7. 2004
    ..In the FhuA deletion mutant, the TonB requirement for phage T1 infection was partially bypassed...
  31. pmc Analysis of the ferric citrate transport gene promoter of Escherichia coli
    Sabine Enz
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    J Bacteriol 185:2387-91. 2003
    ..Titration of FecI suggests that the FecI-RNA polymerase holoenzyme does not bind strongly to the downstream regulatory element, which is therefore probably involved in a subsequent step of transcription initiation...
  32. ncbi request reprint Diffusion through channel derivatives of the Escherichia coli FhuA transport protein
    Michael Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    Eur J Biochem 269:4948-59. 2002
    ..It is concluded that FhuADelta5-160 Delta335-355 assumes a conformation that is incompatible with any of the FhuA functions...
  33. pmc Structural and mechanistic studies of pesticin, a bacterial homolog of phage lysozymes
    Silke I Patzer
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    J Biol Chem 287:23381-96. 2012
    ..Pesticin, a muramidase, was characterized by active site mutations demonstrating a similar but not identical residue pattern in comparison with T4 lysozyme...
  34. pmc Residues involved in FecR binding are localized on one side of the FecA signaling domain in Escherichia coli
    Elena Breidenstein
    Microbiology Membrane Physiology, University of Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 188:6440-2. 2006
    ..Some of the mutants showed strongly reduced iron transport rates, which suggests that the signaling domain affects the structure of the FecA transporter domain...
  35. pmc Crystal structure of colicin M, a novel phosphatase specifically imported by Escherichia coli
    Kornelius Zeth
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, Tubingen, Germany
    J Biol Chem 283:25324-31. 2008
    ..The homology pattern supports our previous proposal that colicins evolved by combination of distinct functional domains...
  36. ncbi request reprint Transcriptional regulation of ferric citrate transport in Escherichia coli K-12. Fecl belongs to a new subfamily of sigma 70-type factors that respond to extracytoplasmic stimuli
    A Angerer
    Universitat Tubingen, Germany
    Mol Microbiol 18:163-74. 1995
    ..These data support the previous assignment of FecI, based on sequence homologies, to a new subfamily of eubacterial RNA polymerase sigma 70 factors that respond to extra-cytoplasmic stimuli and regulate extracytoplasmic functions...
  37. ncbi request reprint SigX of Bacillus subtilis replaces the ECF sigma factor fecI of Escherichia coli and is inhibited by RsiX
    S Brutsche
    Mikrobiologie II, Universitat Tubingen, Germany
    Mol Gen Genet 256:416-25. 1997
    ..It is not known which promoters are recognized by SigX in B. subtilis. SigX may be involved in the regulation of iron metabolism, as evidenced by its activity in E. coli...
  38. pmc ExbBD-dependent transport of maltodextrins through the novel MalA protein across the outer membrane of Caulobacter crescentus
    Heidi Neugebauer
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    J Bacteriol 187:8300-11. 2005
    ..MalA is the first example of an outer membrane protein for which an ExbB/ExbD-dependent transport of a nutrient other than iron and vitamin B(12) has been demonstrated...
  39. pmc In vivo evidence for TonB dimerization
    Annette Sauter
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:5747-54. 2003
    ..They displayed different activity levels for various TonB-dependent functions, suggesting function-related specificities which reflect differences in the interactions of TonB with various transporters and receptors...
  40. pmc The FecI extracytoplasmic-function sigma factor of Escherichia coli interacts with the beta' subunit of RNA polymerase
    Susanne Mahren
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 185:1796-802. 2003
    ..This is the first demonstration of binding of an ECF sigma factor of the FecI type to the beta' subunit of RNA polymerase and of binding being enhanced by the protein that activates the ECF sigma factor...
  41. doi request reprint Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry
    Avijit Pramanik
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    Biochemistry 50:8950-6. 2011
    ....
  42. pmc Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment
    K Kampfenkel
    Universitat Tubingen, Germany
    J Bacteriol 175:4485-91. 1993
    ..The amino acid sequences of these proteins display the highest homology in the transmembrane segments, which indicates that the membrane-spanning regions play an important role in the activities of the proteins...
  43. pmc Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein
    D Welz
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    J Bacteriol 180:2387-94. 1998
    ....
  44. pmc FhuA barrel-cork hybrids are active transporters and receptors
    H Killmann
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    J Bacteriol 183:3476-87. 2001
    ....
  45. ncbi request reprint Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins
    V Braun
    Mikrobiologie II, Universitat Tubingen, Germany
    Mol Microbiol 8:261-8. 1993
    ..Sequence homology and mutual functional substitution of ExbB-ExbD and TolQ-TolR suggest the evolution of the two import systems from a single import system...
  46. pmc Periplasmic chaperone FkpA is essential for imported colicin M toxicity
    Julia Hullmann
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Mol Microbiol 69:926-37. 2008
    ..Colicin M is a suitable tool for the isolation of FkpA mutants used to elucidate the functions of the FkpA N- and C-domains...
  47. ncbi request reprint Regulation of citrate-dependent iron transport of Escherichia coli: fecR is required for transcription activation by FecI
    M Ochs
    Universitat Tubingen, Germany
    Mol Microbiol 15:119-32. 1995
    ..Transcription of the fec transport genes is positively regulated by FecI and FecR and negatively regulated by the Fe2(+)-Fur repressor. Transcription activation and repression may occur independently of each other...
  48. pmc Surface signaling in ferric citrate transport gene induction: interaction of the FecA, FecR, and FecI regulatory proteins
    S Enz
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 182:637-46. 2000
    ....
  49. ncbi request reprint Novel colicin 10: assignment of four domains to TonB- and TolC-dependent uptake via the Tsx receptor and to pore formation
    H Pilsl
    Universitat Tubingen, Germany
    Mol Microbiol 16:57-67. 1995
    ..Col10 consists of 4 domains of which two are very similar and two are very different to E1, supporting our previous proposal that colicins evolved by recombination of DNA fragments which encode uptake and activity domains...
  50. ncbi request reprint Transcription induction of the ferric citrate transport genes via the N-terminus of the FecA outer membrane protein, the Ton system and the electrochemical potential of the cytoplasmic membrane
    I Kim
    Mikrobiologie II, Universitat Tubingen, Germany
    Mol Microbiol 23:333-44. 1997
    ....
  51. ncbi request reprint ATP-dependent ferric hydroxamate transport system in Escherichia coli: periplasmic FhuD interacts with a periplasmic and with a transmembrane/cytoplasmic region of the integral membrane protein FhuB, as revealed by competitive peptide mapping
    A Mademidis
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    Mol Microbiol 26:1109-23. 1997
    ..Although FhuB consists of two homologous halves, FhuB(N) and FhuB(C), the sites identified for FhuD-mediated ferrichrome transport are asymmetrically arranged...
  52. ncbi request reprint The beta-barrel domain of FhuADelta5-160 is sufficient for TonB-dependent FhuA activities of Escherichia coli
    M Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    Mol Microbiol 33:1037-49. 1999
    ....
  53. pmc Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone
    Stephanie Helbig
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    J Biol Chem 286:6280-90. 2011
    ..Weak refolding of Cma(P176A) would explain its low activity in all assays. Of the four proline residues identified as being important for Cma activity, Phe-Pro-176 is most likely targeted by FkpA...
  54. pmc Gene cluster involved in the biosynthesis of griseobactin, a catechol-peptide siderophore of Streptomyces sp. ATCC 700974
    Silke I Patzer
    Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany
    J Bacteriol 192:426-35. 2010
    ..This is the first report identifying the gene cluster for the biosynthesis of DHBA and a catechol siderophore in Streptomyces...
  55. pmc Evidence that the immunity protein inactivates colicin 5 immediately prior to the formation of the transmembrane channel
    H Pilsl
    Universitat Tubingen, Germany
    J Bacteriol 177:6966-72. 1995
    ....
  56. pmc Membrane topology of the Escherichia coli ExbD protein
    K Kampfenkel
    Universitat Tubingen, Germany
    J Bacteriol 174:5485-7. 1992
    ..It is concluded that the only hydrophobic segment of ExbD, encompassing residues 23 to 43, forms a transmembrane domain and that residues 1 to 22 are located in the cytoplasm and residues 44 to 141 are located in the periplasm...
  57. ncbi request reprint Energy-coupled transport through the outer membrane of Escherichia coli small deletions in the gating loop convert the FhuA transport protein into a diffusion channel
    V Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    FEBS Lett 346:59-64. 1994
    ..The gating loop is also involved in binding of all FhuA ligands which in addition to ferrichrome are the phages T1, T5, phi 80, colicin M and the antibiotic albomycin...
  58. pmc Localization of the entire fec region at 97.3 minutes on the Escherichia coli K-12 chromosome
    S Veitinger
    Mikrobiologie II, Universitat Tubingen, Germany
    J Bacteriol 174:3838-9. 1992
  59. ncbi request reprint Activity domains of the TonB protein
    I Traub
    Mikrobiologie II, Universitat Tubingen, Germany
    Mol Microbiol 8:409-23. 1993
    ..The aberrant electrophoretic mobility of TonB was caused by the proline-rich sequence since its removal resulted in a normal mobility...
  60. ncbi request reprint Albomycin is an effective antibiotic, as exemplified with Yersinia enterocolitica and Streptococcus pneumoniae
    Avijit Pramanik
    Microbiology Membrane Physiology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Int J Med Microbiol 297:459-69. 2007
    ..The ferrichrome transport system serves as a Trojan horse to get albomycin into bacteria...
  61. pmc Expression, purification and crystallization of the Cmi immunity protein from Escherichia coli
    Christin Römer
    Department for Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:517-20. 2011
    ..9 Å in the orthorhombic space group C222(1). The crystal packing, with unit-cell parameters a = 66.02, b = 83.47, c = 38.30 Å, indicated the presence of one monomer in the asymmetric unit with a solvent content of 53%...
  62. pmc Iron(III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated membrane association of the FhuC protein and negative complementation of fhuC mutants
    G Schultz-Hauser
    Mikrobiologie II, Universitat Tubingen, Germany
    J Bacteriol 174:2305-11. 1992
    ..It is concluded that the physical interaction between FhuC and FhuB implies a coordinate activity of both proteins in the transport of iron(III) hydroxamates through the cytoplasmic membrane...
  63. pmc CbrA is a flavin adenine dinucleotide protein that modifies the Escherichia coli outer membrane and confers specific resistance to Colicin M
    Stephanie Helbig
    Max Planck Institute for Developmental Biology, Department of Protein Evolution, Tuebingen, Germany
    J Bacteriol 194:4894-903. 2012
    ..The next homologs with the highest identity (over 50%) are found in the anaerobic Clostridium botulinum group 1 and a few other Firmicutes...
  64. pmc NagA-dependent uptake of N-acetyl-glucosamine and N-acetyl-chitin oligosaccharides across the outer membrane of Caulobacter crescentus
    Simone Eisenbeis
    Microbiology Membrane Physiology, Proteome Center, University of Tubingen, Tubingen, Germany
    J Bacteriol 190:5230-8. 2008
    ..We propose that NagA forms a small pore through which GlcNAc specifically diffuses into the periplasm and functions as an energy-coupled transporter for the larger chitin oligosaccharides...
  65. doi request reprint Recent insights into iron import by bacteria
    Volkmar Braun
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Curr Opin Chem Biol 15:328-34. 2011
    ..Here we present an outline of bacterial heme and iron siderophore transport exemplified by a few selected cases in which recent progress in the understanding of the transport mechanisms has been achieved...
  66. pmc The genome of the novel phage Rtp, with a rosette-like tail tip, is homologous to the genome of phage T1
    Andreas Wietzorrek
    Microbiology Membrane Physiology, University of Tubingen, Germany
    J Bacteriol 188:1419-36. 2006
    ..Rtp52 is a putative transcriptional regulator, for which 10 conserved inverted repeats were identified upstream of genes in the Rtp genome. In contrast, the much larger E. coli genome has only one such repeat sequence...
  67. pmc Inactivation of FhuA at the cell surface of Escherichia coli K-12 by a phage T5 lipoprotein at the periplasmic face of the outer membrane
    V Braun
    Universitat Tubingen, Germany
    J Bacteriol 176:4710-7. 1994
    ..Llp solubilized in 1.1% octylglucoside prevented T5 inactivation by FhuA dissolved in octylglucoside...
  68. ncbi request reprint Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli
    K Kampfenkel
    University of Tubingen, Germany
    J Biol Chem 268:6050-7. 1993
    ..Both proteins were lacking positive charges at the inner side of the cytoplasmic membrane which determine the orientation of transmembrane segments...
  69. pmc Ferrichrome transport in Escherichia coli K-12: altered substrate specificity of mutated periplasmic FhuD and interaction of FhuD with the integral membrane protein FhuB
    M R Rohrbach
    Universitat Tubingen, Germany
    J Bacteriol 177:7186-93. 1995
    ..His-tag-FhuD and His-tag-FhuD loaded with aerobactin specifically prevented degradation of FhuB and were cross-linked to FhuB. FhuD loaded with substrate and also FhuD free of substrate were able to interact with FhuB...
  70. pmc Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli
    H Killmann
    Universitat Tubingen, Germany
    EMBO J 12:3007-16. 1993
    ..This implies that in wild-type cells ferrichrome transport through the outer membrane is the rate-limiting step and that TonB, ExbB and ExbD are only required for outer membrane transport...
  71. pmc Mapping functional domains of colicin M
    Stephanie Helbig
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    J Bacteriol 193:815-21. 2011
    ..Our results identify regions in Cma responsible for uptake and activity and support the concept of a three-domain arrangement of Cma...
  72. pmc An aspartate deletion mutation defines a binding site of the multifunctional FhuA outer membrane receptor of Escherichia coli K-12
    H Killmann
    Mikrobiologie II, Universitat Tubingen, Germany
    J Bacteriol 174:3479-86. 1992
    ....
  73. pmc Identification of receptor binding sites by competitive peptide mapping: phages T1, T5, and phi 80 and colicin M bind to the gating loop of FhuA
    H Killmann
    Universitat Tubingen, Germany
    J Bacteriol 177:694-8. 1995
    ..These results suggest that the FhuA gating loop is the target for specific binding of phages T1, T5, and phi 80 and colicin M...
  74. doi request reprint TonB-dependent maltose transport by Caulobacter crescentus
    S Lohmiller
    Microbiology Membrane Physiology, University of Tubingen, Germany
    Microbiology 154:1748-54. 2008
    ..MalY is involved in maltose transport across the cytoplasmic membrane by a presumably ion-coupled mechanism...
  75. pmc Albomycin uptake via a ferric hydroxamate transport system of Streptococcus pneumoniae R6
    Avijit Pramanik
    Mikrobiologie Membranphysiologie, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    J Bacteriol 188:3878-86. 2006
    ..It is concluded that active transport of albomycin mediates the high antibiotic efficacy in S. pneumoniae...
  76. ncbi request reprint The Ton system can functionally replace the TolB protein in the uptake of mutated colicin U
    H Pilsl
    Universitat Tubingen, Germany
    FEMS Microbiol Lett 164:363-7. 1998
    ..The Ton system could obviously replace the TolB function in the uptake of the mutated colicin U. The colicin U derivative is the first colicin whose uptake depends on the Ton and the Tol systems...
  77. pmc Energy-coupled transport across the outer membrane of Escherichia coli: ExbB binds ExbD and TonB in vitro, and leucine 132 in the periplasmic region and aspartate 25 in the transmembrane region are important for ExbD activity
    V Braun
    Mikrobiologie II, Universitat Tubingen, Germany
    J Bacteriol 178:2836-45. 1996
    ..These data further support the concept that TonB, ExbB, and ExbD form a complex in which the energized conformation of TonB opens the channels in the outer membrane receptor proteins...
  78. pmc Strong function-related homology between the pore-forming colicins K and 5
    H Pilsl
    Universitat Tubingen, Germany
    J Bacteriol 177:6973-7. 1995
    ....
  79. pmc Periplasmic location of the pesticin immunity protein suggests inactivation of pesticin in the periplasm
    H Pilsl
    Mikrobiologie II, Universitat Tubingen, Germany
    J Bacteriol 178:2431-5. 1996
    ..In the case of pesticin, recombination may have destroyed the lysis gene, of which only a rudimentary fragment exists on pPCP1...
  80. ncbi request reprint Iron uptake mechanisms and their regulation in pathogenic bacteria
    V Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    Int J Med Microbiol 291:67-79. 2001
    ..The few principal mechanisms that underly the large variety of iron supply systems will be presented, as well as cases, in which defined iron supply systems are related to virulence...
  81. ncbi request reprint Two-step fast protein liquid chromatographic purification of the Serratia marcescens hemolysin and peptide mapping with mass spectrometry
    R Hertle
    Institut für Mikrobiologie II, Universitat Tubingen, Germany
    J Chromatogr B Biomed Sci Appl 737:13-23. 2000
    ..These data imply that ShlB presumably imposes a conformation on ShlA-255 that triggers activity...
  82. pmc Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membrane
    H Pilsl
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
    J Bacteriol 181:3578-81. 1999
    ..By contrast, the predicted receptor binding domain shows no sequence similarities to other colicins. Mutants that lacked the OmpW protein were resistant to colicin S4...
  83. ncbi request reprint Conversion of the coprogen transport protein FhuE and the ferrioxamine B transport protein FoxA into ferrichrome transport proteins
    H Killmann
    Universitat Tubingen, Germany
    FEMS Microbiol Lett 161:59-67. 1998
    ..The properties of FhuA-FoxA hybrids suggest different regions of the two receptors for ferric siderophore uptake...
  84. pmc Identification of a new site for ferrichrome transport by comparison of the FhuA proteins of Escherichia coli, Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans
    H Killmann
    Mikrobiologie Membranphysiologie, Universitat Tubingen, D 72076 Tubingen, Germany
    J Bacteriol 180:3845-52. 1998
    ....
  85. ncbi request reprint Surface signaling: novel transcription initiation mechanism starting from the cell surface
    V Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Arch Microbiol 167:325-31. 1997
    ..The synthesis of the outer membrane receptor PupB, involved in the transport of the ferric pseudobactins BN7 and BN8, is induced by the ferric siderophores and requires PupB and two proteins homologous to FecI and FecR...
  86. pmc Activation of Serratia marcescens hemolysin through a conformational change
    Georg Walker
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
    Infect Immun 72:611-4. 2004
    ..We concluded that ShlB interacts with ShlA during secretion and imposes a conformational change in ShlA to form the active hemolysin...
  87. doi request reprint Intercellular communication by related bacterial protein toxins: colicins, contact-dependent inhibitors, and proteins exported by the type VI secretion system
    Volkmar Braun
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Tubingen, Germany
    FEMS Microbiol Lett 345:13-21. 2013
    ..Despite these common properties, the sequences of toxins and immunity proteins of colicins, CDI systems, and type VI systems show little similarity. ..
  88. ncbi request reprint Urinary infection stones
    K H Bichler
    Department of Urology, Eberhard Karls University of Tubingen, Hoppe Seyler Strasse 3, D 72076 Tubingen, Germany
    Int J Antimicrob Agents 19:488-98. 2002
    ..Good therapy and prophylaxis are possible with present-day treatment modalities...
  89. ncbi request reprint X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin
    Teresa E Clarke
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr N W, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 277:13966-72. 2002
    ....
  90. ncbi request reprint Identification of Fur regulated genes in the bacterial fish pathogen Photobacterium damselae ssp. piscicida using the Fur titration assay
    Carlos R Osorio
    Departamento de Microbioloxia e Parasitoloxia, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Galicia, Spain
    Biometals 17:725-33. 2004
    ..Genes encoding a putative complete iron-regulated outer membrane transport protein and a pseudogene of a transport protein were found. The FURTA assay also revealed iron regulation of the AraC type transcriptional regulation...