Genomes and Genes
- The worm in us - Caenorhabditis elegans as a model of human diseaseRalf Baumeister
Trends Biotechnol 20:147-8. 2002
- An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptorDietmar Dirnberger
Bio3 Bioinformatics and Molecular Genetics Faculty of Biology, Center for Biochemistry and Molecular Cell Research ZBMZ, Faculty of Medicine, University of Freiburg, ZBSA Center for Systems Biology, Martinsried, Germany
Nucleic Acids Res 36:e37. 2008..The present work is the first example of a split-ubiquitin interaction screen using an in-situ expressed receptor of the serpentine class, emphasizing the suitability of the described improvements in the screening protocol...
- SnAvi--a new tandem tag for high-affinity protein-complex purificationUrsula Schäffer
Bioinformatics and Molecular Genetics, ZBMZ, Institute for Biology, ZBSA Center for Systems Biology, Albert Ludwigs Universitaet Freiburg, 79104 Freiburg, Germany
Nucleic Acids Res 38:e91. 2010..elegans for tandem affinity purification of protein complexes, western blotting and also for the in vivo sub-cellular localization of labelled proteins...
- Surveillance-activated defenses block the ROS-induced mitochondrial unfolded protein responseEva D Runkel
Spemann Graduate School of Biology and Medicine, Albert Ludwigs University of Freiburg, Freiburg, Germany
PLoS Genet 9:e1003346. 2013..This suggests that both UPRs may share a common membrane associated mechanism...
- Cell-nonautonomous signaling of FOXO/DAF-16 to the stem cells of Caenorhabditis elegansWenjing Qi
Faculty of Biology, Bioinformatics, and Molecular Genetics, Center for Biochemistry and Molecular Cell Research, Freiburg, Germany
PLoS Genet 8:e1002836. 2012..Our data suggest that a functional balance of DAF-16 activities in different tissues determines longevity and reveals a novel, cell-nonautonomous role of FOXO/DAF-16 to affect stem cells...
- Temperature- and touch-sensitive neurons couple CNG and TRPV channel activities to control heat avoidance in Caenorhabditis elegansShu Liu
Laboratory for Bioinformatics and Molecular Genetics, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg, Germany
PLoS ONE 7:e32360. 2012..In this work, we investigate neurological and molecular aspects of thermonociception using genetic, cell biological and physiological approaches...
- Endocrine signaling in Caenorhabditis elegans controls stress response and longevityRalf Baumeister
Bio 3, Bioinformatics and Molecular Genetics, University of Freiburg, Germany
J Endocrinol 190:191-202. 2006....
- Genes, longevity, and technology: meeting report from the 2nd conference on functional genomics of aging in CreteRalf Baumeister
Laboratory of Bioinformatics and Molecular Genetics, Institute of Biology 3, Albert Ludwig University of Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
Sci Aging Knowledge Environ 2004:pe37. 2004..At a recent conference in Hersonissos, Crete, around 200 internationally renowned experts gathered to discuss techniques and emerging results as the science of aging undergoes a shift toward systems biology...
- C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life spanMaren Hertweck
BioIII, Bioinformatics and Molecular Genetics, University of Freiburg, D 79104 Freiburg, Germany
Dev Cell 6:577-88. 2004..Our data also suggest the existence of a second pathway from DAF-2 to DAF-16 that does not depend on AKT-1, AKT-2, and SGK-1...
- SHC-1/p52Shc targets the insulin/IGF-1 and JNK signaling pathways to modulate life span and stress response in C. elegansElke Neumann-Haefelin
Bioinformatics and Molecular Genetics Faculty of Biology, Center for Biochemistry and Molecular Cell Research Faculty of Medicine, University of Freiburg, D 79104 Freiburg, Germany
Genes Dev 22:2721-35. 2008..elegans SHC-1 as a critical scaffold that directly cross-connects the two parallel JNK and IIS pathways and help to explain how these signaling cascades cooperate to ascertain normal stress response and life span in C. elegans...
- A mutation in CHN-1/CHIP suppresses muscle degeneration in Caenorhabditis elegansOyunbileg Nyamsuren
Bio 3, Bioinformatics and Molecular Genetics Faculty of Biology, Schaenzlestr 1, D 79104 Freiburg, Germany
Dev Biol 312:193-202. 2007..This suggests a critical role of CHIP/CHN-1-mediated ubiquitylation in the control of muscle wasting and degeneration and identifies a potential new drug target for the treatment of this disease...
- Inhibition of tau aggregation in a novel Caenorhabditis elegans model of tauopathy mitigates proteotoxicityChronis Fatouros
Institute of Biology III, University of Freiburg, 79104 Freiburg, Germany
Hum Mol Genet 21:3587-603. 2012..Our study establishes a new C. elegans model of Tau aggregation-mediated toxicity and supports the emerging notion that inhibiting the nucleation of Tau aggregation can be neuroprotective...
- Caenorhabditits elegans LRK-1 and PINK-1 act antagonistically in stress response and neurite outgrowthJulia Sämann
Bioinformatics and Molecular Genetics, Faculty of Biology, Albert Ludwigs Universitaet Freiburg, Freiburg, Germany
J Biol Chem 284:16482-91. 2009....
- Functional and spatial analysis of C. elegans SYG-1 and SYG-2, orthologs of the Neph/nephrin cell adhesion module directing selective synaptogenesisNicola Wanner
Renal Division, University Hospital Freiburg, Freiburg, Germany
PLoS ONE 6:e23598. 2011....
- Automated assays to study longevity in C. elegansMaren Hertweck
Bio3 Bioinformatics and Molecular Genetics, Albert Ludwigs University of Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
Mech Ageing Dev 126:139-45. 2005..elegans. Here, we discuss several methodological approaches to automate longevity assays in the nematode...
- Caenorhabditis elegans as a model system for Parkinson's diseaseEnrico Schmidt
Bioinformatics and Molecular Genetics Faculty of Biology, Center for Biochemistry, University of Freiburg, Freiburg, Germany
Neurodegener Dis 4:199-217. 2007..In this review we describe that C. elegans can be used to advance our understanding of the genetic mechanisms implicated in these disorders...
- Presenilin function in Caenorhabditis elegansAgata Smialowska
Bio3 Bioinformatics and Molecular Genetics, University of Freiburg, Freiburg, Germany
Neurodegener Dis 3:227-32. 2006..Mutations in spr-1, spr-3, spr-4 and spr-5 genes suppress the egg-laying phenotype of sel-12 loss of function mutants by derepressing the expression of the second C. elegans presenilin gene, hop-1...
- Targeted Purification of SnAvi-Tagged ProteinsUrsula Schäffer
Institute for Biology 3, University of Freiburg, Schänzlestr 4, 79104, Freiburg, Germany
Methods Mol Biol 1177:163-74. 2014..Due to its modular composition, this tag is multifunctional and facilitates among others the in vivo visualization of tagged proteins and their cell type specific activation. ..
- The genetics of synapse formation and function in Caenorhabditis elegansMark Seifert
Bio 3, Bioinformatics and Molecular Genetics, University of Freiburg, Schaenzlestrasse 1, 79104 Freiburg Brsg, Germany
Cell Tissue Res 326:273-85. 2006..elegans will be outlined briefly. In addition, we summarize new findings that have added to our understanding of NMJs during the last few years...
- Doxycyclin ameliorates a starvation-induced germline tumor in C. elegans daf-18/PTEN mutant backgroundTim Wolf
Faculty of Biology, Institute of Biology III, Albert Ludwigs University, D 79104 Freiburg, Germany
Exp Gerontol 56:114-22. 2014....
- Inhibition of mTORC1 by astrin and stress granules prevents apoptosis in cancer cellsKathrin Thedieck
Faculty of Biology, Albert Ludwigs University Freiburg, 79104 Freiburg, Germany
Cell 154:859-74. 2013..Importantly, we show that in cancer cells, apoptosis suppression during stress depends on astrin. Being frequently upregulated in tumors, astrin is a potential clinically relevant target to sensitize tumors to apoptosis...
- Perception of UV-B by the Arabidopsis UVR8 proteinLuca Rizzini
Faculty of Biology, Institute of Biology II, University of Freiburg, D 79104 Freiburg, Germany
Science 332:103-6. 2011..Thereby this signaling cascade controlled by UVR8 mediates UV-B photomorphogenic responses securing plant acclimation and thus promotes survival in sunlight...
- C. elegans, a model for aging with high-throughput capacityMaren Hertweck
ABI Molecular Neurogenetics, Ludwig Maximilians University Munich, Schillerstr 44, D 80336 Munich, Germany
Exp Gerontol 38:345-6. 2003..High-throughput analyses have been devised that allow large-scale analysis to identify additional genes, as well as compounds that affect life-span...
- Two suppressors of sel-12 encode C2H2 zinc-finger proteins that regulate presenilin transcription in Caenorhabditis elegansBernard Lakowski
ABI, Department of Biochemistry, Laboratory of Molecular Neurogenetics, Ludwig Maximilians Universitaet, Schillerstr 44, D 80336 Munich, Germany
Development 130:2117-28. 2003....
- Deletion of the intestinal peptide transporter affects insulin and TOR signaling in Caenorhabditis elegansBarbara Meissner
Acolph Butenandt Institute Molecular Neurogenetics, Ludwig Maximilians University of Munich, D 80336 Munich, Germany
J Biol Chem 279:36739-45. 2004....
- ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis elegansGiuseppe Cassata
ABI Molecular Neurogenetics, LMU Munich, 80336 Munich, Germany
Development 132:739-49. 2005....
- Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegansJennifer M A Tullet
Section on Developmental and Stem Cell Biology, Joslin Diabetes Center Department of Pathology, Harvard Medical School Harvard Stem Cell Institute, 1 Joslin Place, Boston, MA 02215, USA
Cell 132:1025-38. 2008..Furthermore, SKN-1 that is constitutively active increases life span independently of DAF-16. Our findings indicate that the transcription network regulated by SKN-1 promotes longevity and is an important direct target of IIS...
- A Caenorhabditis elegans Parkin mutant with altered solubility couples alpha-synuclein aggregation to proteotoxic stressWolfdieter Springer
ABI Molecular Neurogenetics, University of Munich, Germany
Hum Mol Genet 14:3407-23. 2005..Due to high-throughput capacity of C. elegans, this model is particularly well suited to identify genetic and chemical modifiers of toxicity...
- Caenorhabditis elegans neprilysin NEP-1: an effector of locomotion and pharyngeal pumpingBritta Spanier
ABI Molecular Neurogenetics, Ludwig Maximilians University, Munich, Germany
J Mol Biol 352:429-37. 2005..elegans FLP-8), indicating that NEP-1 is a central component that controls the neuronal innervation of pharyngeal pumping in C.elegans...
- The Caenorhabditis elegans presenilin sel-12 is required for mesodermal patterning and muscle functionStefan Eimer
ABI, Department of Biochemistry, Laboratory of Molecular Neurogenetics, Ludwig Maximilians Universitaet Munich, Schillerstrasse 44, Munich D 80336, Germany
Dev Biol 251:178-92. 2002....
- Protein histidine phosphatase: a novel enzyme with potency for neuronal signalingSusanne Klumpp
Institute for Medicinal Chemistry, Westfalische Wilhelms University, Munster, Germany
J Cereb Blood Flow Metab 22:1420-4. 2002..In Caenorhabditis elegans, the homolog of this histidine phosphatase was exclusively expressed in neurons, suggesting a distinct role of reversible histidine phosphorylation in neuronal functions...
- Regulation of the myosin-directed chaperone UNC-45 by a novel E3/E4-multiubiquitylation complex in C. elegansThorsten Hoppe
Department of Molecular Neurogenetics, Adolph Butenandt Institute, Ludwig Maximilians University, Schillerstrasse 44, 80336 Munich, Germany
Cell 118:337-49. 2004..These results identify CHN-1 and UFD-2 as a functional E3/E4 complex and UNC-45 as its physiologically relevant substrate...
- A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor proteinSusanne Schöbel
Center for Integrated Protein Science and the Adolf Butenandt Institut, Ludwig Maximilians University, Munich, Germany
J Biol Chem 283:14257-68. 2008....
- Loss of srf-3-encoded nucleotide sugar transporter activity in Caenorhabditis elegans alters surface antigenicity and prevents bacterial adherenceJörg Höflich
ABI Molecular Neurogenetics, Ludwig Maximilians University, 80336 Munich, Germany
J Biol Chem 279:30440-8. 2004..We propose that the inability of Yersinia biofilms and M. nematophilum to adhere to the nematode cuticle is due to an altered glycoconjugate surface composition of the srf-3 mutant...
- A novel actin barbed-end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegansAssunta Croce
IFOM Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy
Nat Cell Biol 6:1173-9. 2004..Therefore, nematode genetics allowed not only the unmasking of distinct EPS-8-linked phenotypes, but also the definition of a novel function for this molecule in actin dynamics...
- Caenorhabditis elegans MPP+ model of Parkinson's disease for high-throughput drug screeningsEvelyn Braungart
PIERIS Proteolab AG, Freising Weihenstephan, Wurzburg, Germany
Neurodegener Dis 1:175-83. 2004..elegans with a variety of pharmacologically active components used in PD treatment reduced the MPP+-induced defects. Our data suggest that the C. elegans MPTP/MPP+ model can be used for the quantitative evaluation of anti-PD drugs...