Christian Andersen

Summary

Country: Germany

Publications

  1. ncbi The porin RafY encoded by the raffinose plasmid pRSD2 of Escherichia coli forms a general diffusion pore and not a carbohydrate-specific porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Eur J Biochem 254:679-84. 1998
  2. ncbi The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Mol Microbiol 31:499-510. 1999
  3. ncbi Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria
    C Andersen
    Department of Biotechnology, University of Wurzburg, 97074 Wurzburg, Germany
    Rev Physiol Biochem Pharmacol 147:122-65. 2003
  4. ncbi PH-induced collapse of the extracellular loops closes Escherichia coli maltoporin and allows the study of asymmetric sugar binding
    Christian Andersen
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, Germany
    J Biol Chem 277:41318-25. 2002
  5. pmc Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (maltoporin) channel of Escherichia coli. II. Effect on maltose and maltooligosaccharide binding kinetics
    Frank Orlik
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biophys J 83:309-21. 2002
  6. ncbi The channel-tunnel HI1462 of Haemophilus influenzae reveals differences to Escherichia coli TolC
    Georg Polleichtner
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Microbiology 152:1639-47. 2006
  7. pmc Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (Maltoporin) channel of Escherichia coli. I. Effect on ion transport
    Frank Orlik
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biophys J 82:2466-75. 2002
  8. ncbi Importance of the adaptor (membrane fusion) protein hairpin domain for the functionality of multidrug efflux pumps
    Johannes F Stegmeier
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biochemistry 45:10303-12. 2006
  9. ncbi Characterisation of YtfM, a second member of the Omp85 family in Escherichia coli
    Johannes F Stegmeier
    Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biol Chem 388:37-46. 2007
  10. ncbi Characterization of pores formed by YaeT (Omp85) from Escherichia coli
    Johannes F Stegmeier
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Biochem 140:275-83. 2006

Collaborators

Detail Information

Publications18

  1. ncbi The porin RafY encoded by the raffinose plasmid pRSD2 of Escherichia coli forms a general diffusion pore and not a carbohydrate-specific porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Eur J Biochem 254:679-84. 1998
    ..Our results suggest that RafY is a general diffusion pore with a diameter, larger than that of the general diffusion porins OmpF and OmpC, that allows the diffusion of high-molecular-mass carbohydrates through the outer membrane...
  2. ncbi The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Mol Microbiol 31:499-510. 1999
    ..The kinetics of cellopentaose binding to BglH was investigated using the carbohydrate-induced current noise and was compared with that of cellopentaose binding to LamB (maltoporin) and ScrY (sucroseporin)...
  3. ncbi Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria
    C Andersen
    Department of Biotechnology, University of Wurzburg, 97074 Wurzburg, Germany
    Rev Physiol Biochem Pharmacol 147:122-65. 2003
    ..Understanding the mechanism of the different export apparatus could help to develop new drugs, which block the efflux pumps or the secretion system...
  4. ncbi PH-induced collapse of the extracellular loops closes Escherichia coli maltoporin and allows the study of asymmetric sugar binding
    Christian Andersen
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, Germany
    J Biol Chem 277:41318-25. 2002
    ..The results are discussed in respect to possible physiological function of the pH-dependent closure of maltoporin...
  5. pmc Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (maltoporin) channel of Escherichia coli. II. Effect on maltose and maltooligosaccharide binding kinetics
    Frank Orlik
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biophys J 83:309-21. 2002
    ..The results suggest that tyrosine 118 has a crucial effect on carbohydrate transport through LamB...
  6. ncbi The channel-tunnel HI1462 of Haemophilus influenzae reveals differences to Escherichia coli TolC
    Georg Polleichtner
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Microbiology 152:1639-47. 2006
    ..coli TolC in the haemolysin secretion system, but not in the AcrAB/TolC multidrug efflux pump. The structure-function relationship of HI1462 is discussed in the context of the well-studied TolC channel-tunnel of E. coli...
  7. pmc Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (Maltoporin) channel of Escherichia coli. I. Effect on ion transport
    Frank Orlik
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biophys J 82:2466-75. 2002
    ..The individual hydrophobicity indices of the amino acids introduced inside the central constriction of the LamB channel had a somewhat smaller effect on the single-channel conductance as compared with the effect of their size and charge...
  8. ncbi Importance of the adaptor (membrane fusion) protein hairpin domain for the functionality of multidrug efflux pumps
    Johannes F Stegmeier
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biochemistry 45:10303-12. 2006
    ..The model of two hairpin docking sites per TolC protomer corresponding with hexameric adaptor proteins was confirmed by disulfide cross-linking experiments. The role of this interaction for functional efflux pumps is discussed...
  9. ncbi Characterisation of YtfM, a second member of the Omp85 family in Escherichia coli
    Johannes F Stegmeier
    Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biol Chem 388:37-46. 2007
    ..The only observable phenotype was strongly reduced growth, indicating an important role of YtfM in vivo...
  10. ncbi Characterization of pores formed by YaeT (Omp85) from Escherichia coli
    Johannes F Stegmeier
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Biochem 140:275-83. 2006
    ..We could further show that the periplasmic domain proves to be essential for in vivo function of YaeT...
  11. pmc CymA of Klebsiella oxytoca outer membrane: binding of cyclodextrins and study of the current noise of the open channel
    Frank Orlik
    Department of Biotechnology, University of Wurzburg, Wurzburg, Germany
    Biophys J 85:876-85. 2003
    ..Experiments with single CymA-channels demonstrated the channel is highly asymmetric concerning channel flickers and current noise...
  12. ncbi Porin OmpP2 of Haemophilus influenzae shows specificity for nicotinamide-derived nucleotide substrates
    Christian Andersen
    Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, Germany
    J Biol Chem 278:24269-76. 2003
    ..influenzae...
  13. ncbi Dissection of the mechanisms of cytolytic and antibacterial activity of lysenin, a defence protein of the annelid Eisenia fetida
    Heike Bruhn
    Research Center for Infectious Diseases, Biozentrum der Universität Würzburg, Am Hubland, Germany
    Dev Comp Immunol 30:597-606. 2006
    ..Accordingly, lysenin appears to display sphingomyelin-dependent and sphingomyelin-independent activities to kill various foreign intruders of the earthworm's coelomic cavity...
  14. ncbi Molecular and functional characterisation of the Serratia marcescens outer membrane protein Omp1
    Neus Ruiz
    Laboratory of Microbiology Biomedical Research Centre of Bellvitge, University of Barcelona, E 08907 L Hospitalet, Barcelona, Spain
    Biophys Chem 109:215-27. 2004
    ..The positively charged amino acids localised at the beta-strands opposite the external loop L3, which restrict the pore diameter in the porin monomer...
  15. ncbi The cell surface expression of group 2 capsular polysaccharides in Escherichia coli: the role of KpsD, RhsA and a multi-protein complex at the pole of the cell
    Clodagh McNulty
    Faculty of Life Sciences, 1 800 Stopford Building, The University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Mol Microbiol 59:907-22. 2006
    ..In addition, we show that the RhsA protein, of previously unknown function, could be cross-linked to the complex and that a rhsA mutation reduces K5 biosynthesis suggesting a role for RhsA in coupling biosynthesis and export...
  16. pmc Transition to the open state of the TolC periplasmic tunnel entrance
    Christian Andersen
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 99:11103-8. 2002
    ..The results support a model in which transition to the open state of TolC is achieved by an iris-like realignment of the tunnel entrance helices...
  17. ncbi An aspartate ring at the TolC tunnel entrance determines ion selectivity and presents a target for blocking by large cations
    Christian Andersen
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK
    Mol Microbiol 44:1131-9. 2002
    ..The electronegative entrance is widely conserved throughout the TolC family, which is essential for efflux and export my Gram-negative bacteria, suggesting that it could present a general target for drugs...
  18. pmc An RND-type efflux system in Borrelia burgdorferi is involved in virulence and resistance to antimicrobial compounds
    Ignas Bunikis
    Department of Molecular Biology, Umea University, Umea, Sweden
    PLoS Pathog 4:e1000009. 2008
    ..We believe that our findings will be helpful in unraveling the pathogenic mechanisms of borreliae as well as in developing novel therapeutic agents aiming to block the function of this secretion apparatus...