Research Topics
Genomes and Genes | K AltendorfSummaryCountry: Germany Publications
| Collaborators
|
Detail Information
Publications
Structure and function of the Kdp-ATPase of Escherichia coliK Altendorf
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, Germany
Acta Physiol Scand Suppl 643:137-46. 1998..Using this compound labeling of KdpA and KdpB, but not of KdpC, could be shown with the purified complex. When everted vesicles were used only KdpB could be labeled...- Analysis of KdpC of the K(+)-transporting KdpFABC complex of Escherichia coliM Gassel
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, Osnabruck, Germany
Eur J Biochem 268:1772-81. 2001..A simultaneous substitution of both regions was not possible... - The KdpF subunit is part of the K(+)-translocating Kdp complex of Escherichia coli and is responsible for stabilization of the complex in vitroM Gassel
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
J Biol Chem 274:37901-7. 1999..Although KdpF is not essential for the function of the Kdp complex in vivo, it is part of the complex and functions as a stabilizing element in vitro. The corresponding operon should now be referred to as kdpFABC... - Purification, reconstitution, and characterization of KdpD, the turgor sensor of Escherichia coliK Jung
Fachbereich Biologie chemie, Abteilung Mikrobiologie, Universitat Osnabruck, D 49069 Osnabruck, Federal Republic of Germany
J Biol Chem 272:10847-52. 1997..Purified and reconstituted KdpD-His6 exhibited autokinase activity, and the phosphoryl group could be transferred to KdpE. Furthermore, KdpD-His6 was found to be the only protein that mediates dephosphorylation of KdpE approximately P... - Semisynthetic derivatives of concanamycin A and C, as inhibitors of V- and P-type ATPases: structure-activity investigations and developments of photoaffinity probesS Dröse
Fachbereich Biologie chemie, Universitat Osnabruck, Barbarastrasse 11, Arbeitsgruppe Mikrobiologie, D 49076 Osnabrück, Germany
Biochemistry 40:2816-25. 2001..Gassel, M., Ingenhorst, G., Dröse, S., Zeeck, A., Altendorf, K., Wieczorek, H., manuscript submitted)... - Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coliP Voelkner
Universitat Osnabruck, Fachbereich Biologie chemie, Germany
Eur J Biochem 217:1019-26. 1993..Furthermore, replacement of the conserved histidine (His673), the predicted phosphorylation site in KdpD, by glutamine revealed that phosphorylation of KdpD was no longer possible... 
Cs(+) induces the kdp operon of Escherichia coli by lowering the intracellular K(+) concentrationK Jung
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, D 49069 Osnabruck, Germany
J Bacteriol 183:3800-3. 2001..Moreover, the results imply that the signal transduction cascade mediated by KdpD and KdpE is able to integrate multiple signals...- The hydrophilic N-terminal domain complements the membrane-anchored C-terminal domain of the sensor kinase KdpD of Escherichia coliR Heermann
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
J Biol Chem 275:17080-5. 2000..These results highlight the importance of the N-terminal domain for the function of KdpD and provide evidence for an interaction of this domain and the transmitter domain of the sensor kinase... - The turgor sensor KdpD of Escherichia coli is a homodimerR Heermann
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
Biochim Biophys Acta 1415:114-24. 1998..BN-PAGE of solubilized and purified KdpD-6His devoid of Cys residues demonstrates that Cys residues do not contribute to the stabilization of the dimer... - Assembly of the Kdp complex, the multi-subunit K+-transport ATPase of Escherichia coliM Gassel
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
Biochim Biophys Acta 1415:77-84. 1998..A structure in which KdpC could be one of the connecting links between the energy-delivering subunit KdpB and the K+-transporting subunit KdpA is suggested by these data... - Characterization of truncated forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coliW Puppe
Universitat Osnabruck, Fachbereich Biologie chemie, D 49069 Osnabruck, Germany
J Biol Chem 271:25027-34. 1996..Furthermore, kdp expression was investigated using a KdpA-LacZ fusion. The data obtained support the notion that the extent of kdp expression is modulated by the N-terminal part of KdpD... - Effect of cysteine replacements on the properties of the turgor sensor KdpD of Escherichia coliK Jung
Fachbereich Biologie chemie, Abteilung Mikrobiologie, Universitat Osnabruck, D 49069 Osnabruck, Germany
Biochim Biophys Acta 1372:311-22. 1998..Surprisingly, introduction of Cys852 and Cys874 into a KdpD protein devoid of Cys residues leads to full recovery of the kinase activity. Labeling studies support the idea that a disulfide bridge forms between these two residues... - K+ and ionic strength directly influence the autophosphorylation activity of the putative turgor sensor KdpD of Escherichia coliK Jung
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
J Biol Chem 275:40142-7. 2000.... - Overproduction and purification of the uncI gene product of the ATP synthase of Escherichia coliB Schneppe
Universitat Osnabruck, Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, Federal Republic of Germany
J Biol Chem 265:389-95. 1990..E., Saraste, M., and Gay, N. J. (1984) Biochim. Biophys. Acta 768, 164-200), the chromosome-encoded i protein contains the N-terminal sequence Ser-Val-Ser-Leu-Val-Ser-Arg and has a molecular weight of 13,504... - Negatively charged phospholipids influence the activity of the sensor kinase KdpD of Escherichia coliI Stallkamp
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
Arch Microbiol 172:295-302. 1999..These results indicate that electrostatic interactions are important for the activity of KdpD... - Individual substitutions of clustered arginine residues of the sensor kinase KdpD of Escherichia coli modulate the ratio of kinase to phosphatase activityK Jung
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
J Biol Chem 273:26415-20. 1998..Finally, these results lend support to the notion that KdpD can be activated by two distinct stimuli, K+ limitation and osmolarity... - Truncation of amino acids 12-128 causes deregulation of the phosphatase activity of the sensor kinase KdpD of Escherichia coliK Jung
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, D 49069 Osnabruck, Germany
J Biol Chem 273:17406-10. 1998..These results suggest that amino acids 12-128 of KdpD are important for its activity and that an additional ATP-binding site in the N-terminal region seems to be involved in modulation of the phosphatase activity... - The ATP synthase of Escherichia coli: structure and function of F(0) subunitsG Deckers-Hebestreit
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, D 49069, Osnabruck, Germany
Biochim Biophys Acta 1458:364-73. 2000..Consequences on the coupling mechanism between ATP synthesis/hydrolysis and proton translocation are discussed... - Secondary structure composition of reconstituted subunit b of the Escherichia coli ATP synthaseJ C Greie
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, Germany
Eur J Biochem 267:3040-8. 2000.... - Detection and localization of the i protein in Escherichia coli cells using antibodiesB Schneppe
Universitat Osnabruck, Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, Germany
FEBS Lett 292:145-7. 1991..The i protein was identified as a component of the cytoplasmic membrane and shown to be present in preparations of Fo or F1Fo. The protein is not associated with the F1 moiety... - Membrane topology analysis of the sensor kinase KdpD of Escherichia coliP Zimmann
Universitat Osnabruck, Fachbereich Biologie chemie, Federal Republic of Germany
J Biol Chem 270:28282-8. 1995..The results revealed that KdpD has four membrane-spanning segments in the middle of the polypeptide chain, whereas N and C terminus are both cytoplasmic... - The phosphorylation site of the Kdp-ATPase of Escherichia coli: site-directed mutagenesis of the aspartic acid residues 300 and 307 of the KdpB subunitW Puppe
Universitat Osnabruck, Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, Germany
Mol Microbiol 6:3511-20. 1992..However, the Km for K+ of the ATPase activity has been increased 30-fold compared with the wild-type enzyme... - F0 portion of Escherichia coli ATP synthase: orientation of subunit c in the membraneG Deckers-Hebestreit
Fachbereich Biologie chemie, Mikrobiologie, Universitat Osnabruck, FRG
Biochemistry 26:5486-92. 1987.... - Turnover number of Escherichia coli F0F1 ATP synthase for ATP synthesis in membrane vesiclesC Etzold
Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, Universitat Osnabruck, Germany
Eur J Biochem 243:336-43. 1997..Therefore, these studies demonstrate that the ATP synthase complex of E. coli has, with respect to maximum rates, the same capacity as the corresponding enzymes of eukaryotic organells... - Subunit organization of the stator part of the F0 complex from Escherichia coli ATP synthaseJ C Greie
Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, Germany
J Bioenerg Biomembr 32:357-64. 2000..This ab2 subcomplex was shown to be active in proton translocation and F1 binding, when coreconstituted with subunit c. Consequences of crosslink formation and subunit interaction within the F1F0 complex are discussed... - Identification of bacterial isolates from biofilters as Paracoccus alkenifer sp. nov. and Paracoccus solventivorans with emended description of Paracoccus solventivoransA Lipski
Abteilung Mikrobiologie, Universitat Osnabruck, Germany
Int J Syst Bacteriol 48:529-36. 1998..alkenifer and Paracoccus kocurii. By means of a GC-MS method, diaminopimelic acid was detected for P. solventivorans. Based on these results we propose an emended description for the species P. solventivorans... - Translation of the first gene of the Escherichia coli unc operon. Selection of the start codon and control of initiation efficiencyB Schneppe
Universitat Osnabruck, Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, Federal Republic of Germany
J Biol Chem 266:21090-8. 1991..The relationship between mRNA sequence and higher order structure on the one hand, and initiation site selection and initiation efficiency on the other, was analyzed... - Prolonged survival and cytoplasmic pH homeostasis of Helicobacter pylori at pH 1K Stingl
Abteilung Mikrobiologie, Universitat Osnabruck, D 49069 Osnabruck, Germany
Infect Immun 69:1178-80. 2001..Under these conditions, the cells maintained their cytoplasmic pH at 5.8. De novo protein synthesis during acid shock was not essential for survival of H. pylori at pH 1... - Fatty acid profiles of nitrite-oxidizing bacteria reflect their phylogenetic heterogeneityA Lipski
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, Germany
Syst Appl Microbiol 24:377-84. 2001..A cluster analysis of the fatty acid profiles is in accordance with 16S rRNA sequence-based phylogeny of the nitrite-oxidizing bacteria... - Application of rRNA-targeted oligonucleotide probes in biotechnologyA Lipski
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, Germany
Appl Microbiol Biotechnol 56:40-57. 2001..This review is intended to present a summary of methodological aspects of rRNA-based hybridization techniques and their application in biotechnology... - Diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican cropsC Rodriguez
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, Barbarastrasse 11, Osnabruck, Germany
J Appl Microbiol 103:2550-60. 2007..To ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops... - Characterization of the phosphorylated intermediate of the K+-translocating Kdp-ATPase from Escherichia coliA Siebers
Arbeitsgruppe Mikrobiologie, Universitat Osnabruck, Federal Republic of Germany
J Biol Chem 264:5831-8. 1989..The KdpB polypeptide was identified as the phosphorylated subunit after electrophoretic separation at pH 2.4, 4 degrees C of cytoplasmic membranes or of purified ATPase labeled with [gamma-32P]ATP... - The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coliK Altendorf
, Fachbereich Biologie/Chemie, , Germany
Res Microbiol 145:374-81. 1994 - The effects of an atpE ribosome-binding site mutation on the stoichiometry of the c subunit in the F1F0 ATPase of Escherichia coliR A Schemidt
Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Arch Biochem Biophys 323:423-8. 1995..Therefore, the stoichiometry of the c subunit assembled into the F1F0 complex appears to be variable, depending on the expression of atpE... - Amino acid replacement in dicyclohexylcarbodiimide-reactive proteins from mutant strains of Escherichia coli defective in the energy-transducing ATPase complexE Wachter
FEBS Lett 113:265-70. 1980 - Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulumJ E Hesse
Proc Natl Acad Sci U S A 81:4746-50. 1984..The phosphorylated aspartate residue of the latter is within a region of homology... - Purification and properties of a periplasmic protein related to sn-glycerol-3-phosphate transport in Escherichia coliW Boos
Eur J Biochem 72:571-81. 1977..coli... - The KDP ATPase of Escherichia coliK Altendorf
Department of Microbiology, University of Osnabrueck, Germany
Ann N Y Acad Sci 671:228-43. 1992 - The K+-translocating Kdp-ATPase from Escherichia coli. Purification, enzymatic properties and production of complex- and subunit-specific antiseraA Siebers
Universitat Osnabruck, Federal Republic of Germany
Eur J Biochem 178:131-40. 1988..In functional inhibition studies the anti-KdpABC and anti-KdpB sera impaired ATPase activity in the membrane-bound as well as in the purified state of the enzyme. In contrast, the anti-KdpC serum did not inhibit enzyme activity... - Modulation of KdpD phosphatase implicated in the physiological expression of the kdp ATPase of Escherichia coliL Brandon
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
Mol Microbiol 38:1086-92. 2000..The data also suggest that levels of activity in vitro may differ from what occurs in vivo, because in vitro conditions cannot replicate those in vivo... - KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulatorsM O Walderhaug
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637
J Bacteriol 174:2152-9. 1992..Since several other sensor-effectors have been shown to mediate control through phosphorylation, this mechanism is proposed to control expression of Kdp... - The Fo complex of the proton-translocating F-type ATPase of Escherichia coliG Deckers-Hebestreit
Universitat Osnabruck, Fachbereich Biologie chemie, Arbeitsgruppe Mikrobiologie, FRG
J Exp Biol 172:451-9. 1992..Binding studies with a monoclonal antibody against this epitope are now under investigation to determine the orientation of subunit a.(ABSTRACT TRUNCATED AT 250 WORDS)..