Sascha Al Dahouk

Summary

Country: Germany

Publications

  1. ncbi request reprint Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Crit Rev Microbiol 31:191-6. 2005
  2. ncbi request reprint Evaluation of Brucella MLVA typing for human brucellosis
    Sascha Al Dahouk
    Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstr 11, D 80937 Munich, Germany
    J Microbiol Methods 69:137-45. 2007
  3. ncbi request reprint Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem Lab Med 45:1464-70. 2007
  4. ncbi request reprint Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    J Med Microbiol 57:64-71. 2008
  5. doi request reprint Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum-Brucella group by recA and 16S rRNA gene-based comparative sequence analysis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 31:1-16. 2008
  6. pmc Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277, Berlin Germany
    BMC Microbiol 13:199. 2013
  7. ncbi request reprint New developments in the diagnostic procedures for zoonotic brucellosis in humans
    S Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    Rev Sci Tech 32:177-88. 2013
  8. pmc Intraspecies biodiversity of the genetically homologous species Brucella microti
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Berlin, Germany
    Appl Environ Microbiol 78:1534-43. 2012
  9. pmc MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
    Marianne Maquart
    INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, F 37380, France
    BMC Microbiol 9:145. 2009
  10. pmc Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR
    Herbert Tomaso
    Friedrich Loeffler Institut, Institute of Bacterial Infections and Zoonoses, Naumburgerstrasse 96a, 07743 Jena, Germany
    BMC Infect Dis 10:100. 2010

Detail Information

Publications42

  1. ncbi request reprint Identification of brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Crit Rev Microbiol 31:191-6. 2005
    ..Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed...
  2. ncbi request reprint Evaluation of Brucella MLVA typing for human brucellosis
    Sascha Al Dahouk
    Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstr 11, D 80937 Munich, Germany
    J Microbiol Methods 69:137-45. 2007
    ..MLVA can significantly contribute to epidemiological trace-back analysis of Brucella infections and may advance surveillance and control of human brucellosis...
  3. ncbi request reprint Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem Lab Med 45:1464-70. 2007
    ..We therefore assessed the performance of real-time PCR assays for the identification of members of the genus Brucella to the genus and species level...
  4. ncbi request reprint Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    J Med Microbiol 57:64-71. 2008
    ..The developed assay closes a diagnostic gap and provides a simple but robust tool for the sensitive detection and correct identification of O. anthropi, O. intermedium and Brucella spp...
  5. doi request reprint Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum-Brucella group by recA and 16S rRNA gene-based comparative sequence analysis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 31:1-16. 2008
    ..In summary, Ochrobactrum is a highly diverse genus comprising several novel species. We recommend recA- in addition to rrs gene-analysis for correct species allocation and subtyping of novel Ochrobactrum isolates...
  6. pmc Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277, Berlin Germany
    BMC Microbiol 13:199. 2013
    ..In an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined...
  7. ncbi request reprint New developments in the diagnostic procedures for zoonotic brucellosis in humans
    S Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    Rev Sci Tech 32:177-88. 2013
    ..This article reviews the latest microbiological methods for the diagnosis of human brucellosis and outlines developments for the future...
  8. pmc Intraspecies biodiversity of the genetically homologous species Brucella microti
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Berlin, Germany
    Appl Environ Microbiol 78:1534-43. 2012
    ..In summary, the adaptation of Brucella microti to a specific habitat or host seems to be a matter of gene regulation rather than a matter of gene configuration...
  9. pmc MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
    Marianne Maquart
    INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, F 37380, France
    BMC Microbiol 9:145. 2009
    ..A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used...
  10. pmc Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR
    Herbert Tomaso
    Friedrich Loeffler Institut, Institute of Bacterial Infections and Zoonoses, Naumburgerstrasse 96a, 07743 Jena, Germany
    BMC Infect Dis 10:100. 2010
    ..Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA...
  11. doi request reprint Implications of laboratory diagnosis on brucellosis therapy
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Hygiene and Microbiology, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    Expert Rev Anti Infect Ther 9:833-45. 2011
    ..The aim of this article is to review and discuss the implications of laboratory test results in the diagnosis of human brucellosis on disease therapy...
  12. pmc Changing epidemiology of human brucellosis, Germany, 1962-2005
    Sascha Al Dahouk
    Rheinisch Westfälische Technische Hochschule Aachen University, Aachen, Germany
    Emerg Infect Dis 13:1895-900. 2007
    ..Prolonged diagnostic delays and high case fatality call for targeted public health measures...
  13. pmc Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system
    Sascha Al Dahouk
    Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    BMC Microbiol 10:269. 2010
    ....
  14. doi request reprint Brucella inopinata sp. nov., isolated from a breast implant infection
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Int J Syst Evol Microbiol 60:801-8. 2010
    ..Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed...
  15. doi request reprint Brucella microti sp. nov., isolated from the common vole Microtus arvalis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Int J Syst Evol Microbiol 58:375-82. 2008
    ..nov. with the type strain CCM 4915(T) (=BCCN 07-01(T)=CAPM 6434(T)) is proposed...
  16. doi request reprint Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Munich, Germany
    Vector Borne Zoonotic Dis 9:153-6. 2009
    ..Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles...
  17. ncbi request reprint Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assay
    Holger C Scholz
    Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Diagn Microbiol Infect Dis 54:241-7. 2006
    ..mallei infection. The developed PCR assay can be used as a simple and rapid tool for the specific and sensitive detection of B. mallei in clinical samples...
  18. ncbi request reprint Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis
    Holger C Scholz
    Bundeswehr Institute of Microbiology, Munich, Germany
    FEMS Microbiol Lett 257:7-16. 2006
    ..In summary, recA-sequence analysis provides a new reliable molecular subtyping tool to study the phylogeny of the Ochrobactrum taxon at both the inter- and intraspecies level...
  19. doi request reprint Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plague
    Julia M Riehm
    Bundeswehr Institute of Microbiology, Munich, Germany
    Mol Cell Probes 25:8-12. 2011
    ..In conclusion, the 5'-nuclease assay targeting pla can be recommended as diagnostic tool for establishing a presumptive diagnosis when bubonic plague is clinically suspected...
  20. ncbi request reprint The detection of Brucella spp. using PCR-ELISA and real-time PCR assays
    Sascha Al Dahouk
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Clin Lab 50:387-94. 2004
    ..Advantages and disadvantages of the different methods for real-time detection of Brucella spp. and a newly developed PCR-ELISA system for the high throughput of clinical samples will be discussed...
  21. ncbi request reprint Identification and antimicrobial susceptibilities of Ochrobactrum spp
    Bryan Thoma
    Bundeswehr Institute of Microbiology, Munich, Germany
    Int J Med Microbiol 299:209-20. 2009
    ..Our in vitro data suggest that combinations of antimicrobial agents including ciprofloxacin and/or trimethoprim/sulfamethoxazole may be useful for empirical treatment of Ochrobactrum infections...
  22. pmc Brucella endocarditis in prosthetic valves
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Can J Cardiol 22:971-4. 2006
    ..The patient was successfully treated by a medical and surgical approach. Therapeutic strategies in Brucella endocarditis are discussed in light of the current literature...
  23. ncbi request reprint Laboratory-based diagnosis of brucellosis--a review of the literature. Part II: serological tests for brucellosis
    Sascha Al Dahouk
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Clin Lab 49:577-89. 2003
    ..This review will summarize advantages and disadvantages of the serological techniques used in clinical laboratories for indirect verification of human brucellosis...
  24. ncbi request reprint Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensis
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Mol Cell Probes 21:12-6. 2007
    ..This is a major diagnostic improvement, as all other methods for the specific identification of F. tularensis subsp. tularensis are more time consuming...
  25. ncbi request reprint Detection of Chromobacterium violaceum by multiplex PCR targeting the prgI, spaO, invG, and sipB genes
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 29:45-8. 2006
    ..The detection limit for pure cultures in multiplex PCR analysis was 100CFU. The developed assay significantly improves rapid identification of Chr. violaceum and allows its differentiation from closely related organisms...
  26. ncbi request reprint Failure of a short-term antibiotic therapy for human brucellosis using ciprofloxacin. A study on in vitro susceptibility of Brucella strains
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Munich, Germany
    Chemotherapy 51:352-6. 2005
    ..Human brucellosis is characterized byfocal complications, chronic courses, and therapeutic failures...
  27. ncbi request reprint Development of a 5'-nuclease real-time PCR assay targeting fliP for the rapid identification of Burkholderia mallei in clinical samples
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem 52:307-10. 2006
    ..The number of reported outbreaks has increased steadily during the last decade, but diagnosis is hampered by the low bacterial load in infected tissues and excretions...
  28. ncbi request reprint Laboratory-based diagnosis of brucellosis--a review of the literature. Part I: Techniques for direct detection and identification of Brucella spp
    Sascha Al Dahouk
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Clin Lab 49:487-505. 2003
    ..This review will summarize advantages and disadvantages of the techniques used in clinical laboratories for direct detection and identification of Brucella spp...
  29. ncbi request reprint Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis
    Holger Christian Scholz
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    FEMS Microbiol Lett 244:347-52. 2005
    ..In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level...
  30. pmc Molecular epidemiology of Brucella genotypes in patients at a major hospital in central Peru
    Karsten Nockler
    Federal Institute for Risk Assessment, Berlin, Germany
    J Clin Microbiol 47:3147-55. 2009
    ..006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds...
  31. ncbi request reprint Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections
    Sascha Al Dahouk
    Department of Bacteriology, Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    J Immunol Methods 309:34-47. 2006
    ..abortus clearly from cross-reacting bacteria. The combination of SELDI-MS analysis with the specificity of antibody binding will improve the identification of Brucella specific immunogenic proteins...
  32. ncbi request reprint Growth characteristics of Bacillus anthracis compared to other Bacillus spp. on the selective nutrient media Anthrax Blood Agar and Cereus Ident Agar
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D 80937 Munich, Germany
    Syst Appl Microbiol 29:24-8. 2006
    ..Species which might be misidentified as B. anthracis were B. cereus, B. mycoides, and B. thuringiensis. Particularly, 30% of B. weihenstephanensis strains were misidentified as B. anthracis...
  33. ncbi request reprint Antimicrobial susceptibilities of Austrian Francisella tularensis holarctica biovar II strains
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Int J Antimicrob Agents 26:279-84. 2005
    ..Our in vitro susceptibility data can be applied for the detection and comparison of resistance development and to provide in vitro data for the guidance of therapy...
  34. ncbi request reprint Optimized application of surface-enhanced laser desorption/ionization time-of-flight MS to differentiate Francisella tularensis at the level of subspecies and individual strains
    Eric Seibold
    Bundeswehr Institute of Microbiology, Neuherbergstr, Munchen, Germany
    FEMS Immunol Med Microbiol 49:364-73. 2007
    ..Furthermore, single strains within F. tularensis subspecies could be discriminated...
  35. doi request reprint Preliminary validation of real-time PCR assays for the identification of Yersinia pestis
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem Lab Med 46:1239-44. 2008
    ..Therefore, rapid and reliable diagnostic tools are crucial. The objective of this study was to assess the inter-laboratory reproducibility of in-house developed real-time PCR assays for the identification of Y. pestis...
  36. ncbi request reprint Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex
    Herbert Tomaso
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Diagn Mol Pathol 13:247-53. 2004
    ..mallei/B. pseudomallei complex in positive blood cultures or from suspicious bacterial colonies allowing the early onset of appropriate antibiotic therapy...
  37. ncbi request reprint Comparison of four commercially available assays for the detection of IgM phase II antibodies to Coxiella burnetii in the diagnosis of acute Q fever
    Dimitrios Frangoulidis
    Bundeswehr Institute of Microbiology, Neuherbergstr 11, 80937 Munich, Germany
    Ann N Y Acad Sci 1078:561-2. 2006
    ....
  38. pmc Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay
    Philippe Le Fleche
    Centre d Etudes du Bouchet BP3, 91710 Vert le Petit, France
    BMC Microbiol 6:9. 2006
    ..More efficient DNA-based methods are needed, and this work explores the suitability of multiple locus variable number tandem repeats analysis (MLVA) for both typing and species identification...
  39. ncbi request reprint Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes
    Herbert Tomaso
    Federal Armed Forces Institute of Microbiology, Neuherbergstrasse 11, Munich 80937, Germany
    Mol Cell Probes 19:9-20. 2005
    ..In conclusion, we recommend the use of the 16S rDNA and/or fliC real-time PCR assays for the rapid identification of B. mallei and B. pseudomallei in positive blood cultures or from suspicious bacterial colonies...
  40. ncbi request reprint Brucellosis of the lung: case report and review of the literature
    Dirk Theegarten
    Institute of Pathology and Neuropathology, University Hospital Essen, University of Duisburg Essen, Hufelandstrasse 55, Essen, Germany
    Virchows Arch 452:97-101. 2008
    ..In conclusion, granulomatous inflammation negative for Ziehl-Neelsen and Grocott stains presenting together with other localized lesions should lead to specific investigations on brucellosis...
  41. ncbi request reprint Rapid detection of Yersinia pestis with multiplex real-time PCR assays using fluorescent hybridisation probes
    Herbert Tomaso
    Military Hospital Innsbruck, Ministry of Defence, Innsbruck, Austria
    FEMS Immunol Med Microbiol 38:117-26. 2003
    ..We developed and evaluated a highly specific real-time PCR strategy for the detection of Y. pestis, obtaining results within 3 h including DNA preparation...
  42. pmc Isolation of Brucella microti from soil
    Holger C Scholz
    Emerg Infect Dis 14:1316-7. 2008