Research Topics
| Winfried DenkSummaryAffiliation: Abteilung Zellphysiologie Country: Germany Publications
| Collaborators
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Detail Information
Publications
Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivoTanjew Dittgen
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 101:18206-11. 2004..This approach is ideally suited for analysis of gene functions in individual neurons in the intact brain...
Structural neurobiology: missing link to a mechanistic understanding of neural computationWinfried Denk
Max Planck Institute for Medical Research, Heidelberg 69120, Germany
Nat Rev Neurosci 13:351-8. 2012..Given that the brain's algorithms are ultimately encoded by this network, knowing where all of these connections are should, at the very least, provide the data needed to distinguish between models of neural computation...- Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructureWinfried Denk
Max Planck Institute for Medical Research, Heidelberg, Germany
PLoS Biol 2:e329. 2004..This opens the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits... 
Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent developmentShoji Komai
Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Nat Neurosci 9:1125-33. 2006..We conclude that somatodendritic excitability of the postsynaptic neuron is needed for the regulation of synaptic strength in the developing sensory cortex...- High-accuracy neurite reconstruction for high-throughput neuroanatomyMoritz Helmstaedter
Max Planck Institute for Medical Research, Heidelberg, Germany
Nat Neurosci 14:1081-8. 2011..Focused reannotation of difficult locations promises a rather steep increase of reliability as a function of the average skeleton redundancy and thus the nearly error-free analysis of large neuroanatomical datasets... - Wiring specificity in the direction-selectivity circuit of the retinaKevin L Briggman
Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg 69120, Germany
Nature 471:183-8. 2011..Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy... - 3D structural imaging of the brain with photons and electronsMoritz Helmstaedter
Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg, Germany
Curr Opin Neurobiol 18:633-41. 2008..Both photon-based and electron-based imaging methods will ultimately need highly automated data analysis, because the manual tracing of most networks of interest would require hundreds to tens of thousands of years in human labor... - A dendrite-autonomous mechanism for direction selectivity in retinal starburst amacrine cellsSusanne E Hausselt
Department of Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
PLoS Biol 5:e185. 2007.... - Directionally selective calcium signals in dendrites of starburst amacrine cellsThomas Euler
Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
Nature 418:845-52. 2002..This demonstrates that direction selectivity is computed locally in dendritic branches at a stage before ganglion cells... - High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edgeBernd Kuhn
Department for Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
Biophys J 87:631-9. 2004..Such fractional sensitivities are approximately 5 times larger than what is commonly found with other voltage-sensing dyes and approach the theoretical limit given by the spectral Boltzmann tail... - Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensingMarkus Rueckel
Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 103:17137-42. 2006..CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds... - Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifierPatrick Theer
Max Planck Institute for Medical Research, Johnstrasse 29, D 69120 Heidelberg, Germany
Opt Lett 28:1022-4. 2003..The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence... - New developments in multiphoton microscopyFritjof Helmchen
Abt Zellphysiologie, Max Planck Institut fur medizinische Forschung, Jahnstrasse 29, Heidelberg, Germany
Curr Opin Neurobiol 12:593-601. 2002..In addition, nonlinear optical contrast mechanisms other than multiphoton excitation of fluorescence are being explored... - Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET controlMazahir T Hasan
Max Planck Institute for Medical Research, Heidelberg, Germany
PLoS Biol 2:e163. 2004.... - Eyecup scope--optical recordings of light stimulus-evoked fluorescence signals in the retinaThomas Euler
Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstr 29, 69120 Heidelberg, Germany
Pflugers Arch 457:1393-414. 2009..Spatially resolved changes in calcium-indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons... - Coherence-gated wave-front sensing in strongly scattering samplesMarcus Feierabend
Max Planck Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany
Opt Lett 29:2255-7. 2004..Applications are expected for multiphoton and confocal laser-scanning microscopy... - Properties of coherence-gated wavefront sensingMarkus Rueckel
Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
J Opt Soc Am A Opt Image Sci Vis 24:3517-29. 2007..We show, in particular, that all aberrations needed for adaptive wavefront restoration are correctly sensed if circularly polarized light is used... - On the fundamental imaging-depth limit in two-photon microscopyPatrick Theer
Max Planck Institute for Medical Research, Heidelberg, Germany
J Opt Soc Am A Opt Image Sci Vis 23:3139-49. 2006.... - Targeted whole-cell recordings in the mammalian brain in vivoTroy W Margrie
Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Neuron 39:911-8. 2003..TPTP applied in vivo will therefore provide new insights into the molecular control of neuronal function at the systems level... - Spatiotemporally graded NMDA spike/plateau potentials in basal dendrites of neocortical pyramidal neuronsGuy Major
School of Biosciences, Cardiff University, Museum Avenue, Cardiff, Wales, UK
J Neurophysiol 99:2584-601. 2008..Dendritic NMDA spike/plateaus are thus well-suited to support graded persistent firing... - Contour-propagation algorithms for semi-automated reconstruction of neural processesJakob H Macke
Max Planck Institute for Biological Cybernetics, Spemannstrasse 38, D 72076 Tubingen, Germany
J Neurosci Methods 167:349-57. 2008..Compared to purely manual tracing, processing time is greatly accelerated... - Two-photon targeted patching (TPTP) in vivoShoji Komai
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara 630-0192, Japan
Nat Protoc 1:647-52. 2006..This technique may be repeated in the same preparation many times over the course of several hours and is equally applicable to non-neuronal cell types... - Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesisWulf Haubensak
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Proc Natl Acad Sci U S A 101:3196-201. 2004..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex... - Functional organization of sensory input to the olfactory bulb glomerulus analyzed by two-photon calcium imagingMatt Wachowiak
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Proc Natl Acad Sci U S A 101:9097-102. 2004..Hence, sensory inputs to individual glomeruli are spatially heterogeneous but seem to be functionally indiscriminate. These results support the view of olfactory glomeruli as functional units in representing sensory information... - Axons and synaptic boutons are highly dynamic in adult visual cortexDan D Stettler
The Rockefeller University, New York, New York, USA
Neuron 49:877-87. 2006..Bouton losses and gains were both approximately 7% of the total population per week, with no net change in the overall density. These results suggest ongoing processes of synaptogenesis and elimination in adult V1... - Imaging in vivo: watching the brain in actionJason N D Kerr
Network Imaging Group, Max Planck Institute for Biological Cybernetics, Spemannstrasse 41, D 72076 Tuebingen, Germany
Nat Rev Neurosci 9:195-205. 2008..Recently, the limits of what can be achieved in vivo have been pushed into terrain that was previously only accessible in vitro, due to advances in both physical-imaging technology and the design of molecular contrast agents... - The three-dimensional micro- and nanostructure of the aortic medial lamellar unit measured using 3D confocal and electron microscopy imagingMary K O'Connell
Department of Mechanical Engineering, Stanford University, Stanford, CA, USA
Matrix Biol 27:171-81. 2008..This high resolution three-dimensional view of the aortic media reveals MLU microstructure details that suggest a highly complex and integrated mural organization that correlates with aortic mechanical properties... - Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivoKazuo Kitamura
Wolfson Institute for Biomedical Research and Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
Nat Methods 5:61-7. 2008..This approach facilitates the recording, labeling and genetic manipulation of single neurons in the intact native mammalian brain without the need to pre-label neuronal populations... - Long duration three-dimensional imaging of calcium waves in zebrafish using multiphoton fluorescence microscopyEdwin Gilland
NYU School of Medicine, New York, NY, USA
Biol Bull 205:176-7. 2003 - Alpha-herpesvirus infection induces the formation of nuclear actin filamentsBecket Feierbach
Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
PLoS Pathog 2:e85. 2006..Our results suggest a mechanism by which assembly domains are organized within infected cells and provide insight into how the viral infectious cycle and host actin cytoskeleton are integrated to promote the infection process... - Deep tissue two-photon microscopyFritjof Helmchen
Department of Neurophysiology, Brain Research Institute, University of Zurich, CH 8057 Zurich, Switzerland
Nat Methods 2:932-40. 2005..Here we review fundamental concepts of nonlinear microscopy and discuss conditions relevant for achieving large imaging depths in intact tissue... - Towards neural circuit reconstruction with volume electron microscopy techniquesKevin L Briggman
Max-Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Curr Opin Neurobiol 16:562-70. 2006..Such reconstructions of complete neural wiring diagrams could rekindle the tradition of relating neural function to the underlying neuroanatomical circuitry...