Winfried Denk

Summary

Affiliation: Abteilung Zellphysiologie
Country: Germany

Publications

  1. pmc Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
    Tanjew Dittgen
    Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 101:18206-11. 2004
  2. doi request reprint Structural neurobiology: missing link to a mechanistic understanding of neural computation
    Winfried Denk
    Max Planck Institute for Medical Research, Heidelberg 69120, Germany
    Nat Rev Neurosci 13:351-8. 2012
  3. pmc Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure
    Winfried Denk
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 2:e329. 2004
  4. ncbi request reprint Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
    Shoji Komai
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Nat Neurosci 9:1125-33. 2006
  5. doi request reprint High-accuracy neurite reconstruction for high-throughput neuroanatomy
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, Heidelberg, Germany
    Nat Neurosci 14:1081-8. 2011
  6. doi request reprint Wiring specificity in the direction-selectivity circuit of the retina
    Kevin L Briggman
    Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg 69120, Germany
    Nature 471:183-8. 2011
  7. doi request reprint 3D structural imaging of the brain with photons and electrons
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg, Germany
    Curr Opin Neurobiol 18:633-41. 2008
  8. pmc A dendrite-autonomous mechanism for direction selectivity in retinal starburst amacrine cells
    Susanne E Hausselt
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 5:e185. 2007
  9. doi request reprint Connectomic reconstruction of the inner plexiform layer in the mouse retina
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, D 69120 Heidelberg, Germany
    Nature 500:168-74. 2013
  10. ncbi request reprint Directionally selective calcium signals in dendrites of starburst amacrine cells
    Thomas Euler
    Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Nature 418:845-52. 2002

Collaborators

Detail Information

Publications34

  1. pmc Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
    Tanjew Dittgen
    Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 101:18206-11. 2004
    ..This approach is ideally suited for analysis of gene functions in individual neurons in the intact brain...
  2. doi request reprint Structural neurobiology: missing link to a mechanistic understanding of neural computation
    Winfried Denk
    Max Planck Institute for Medical Research, Heidelberg 69120, Germany
    Nat Rev Neurosci 13:351-8. 2012
    ..Given that the brain's algorithms are ultimately encoded by this network, knowing where all of these connections are should, at the very least, provide the data needed to distinguish between models of neural computation...
  3. pmc Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure
    Winfried Denk
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 2:e329. 2004
    ..This opens the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits...
  4. ncbi request reprint Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
    Shoji Komai
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Nat Neurosci 9:1125-33. 2006
    ..We conclude that somatodendritic excitability of the postsynaptic neuron is needed for the regulation of synaptic strength in the developing sensory cortex...
  5. doi request reprint High-accuracy neurite reconstruction for high-throughput neuroanatomy
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, Heidelberg, Germany
    Nat Neurosci 14:1081-8. 2011
    ..Focused reannotation of difficult locations promises a rather steep increase of reliability as a function of the average skeleton redundancy and thus the nearly error-free analysis of large neuroanatomical datasets...
  6. doi request reprint Wiring specificity in the direction-selectivity circuit of the retina
    Kevin L Briggman
    Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg 69120, Germany
    Nature 471:183-8. 2011
    ..Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy...
  7. doi request reprint 3D structural imaging of the brain with photons and electrons
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, Department of Biomedical Optics, Heidelberg, Germany
    Curr Opin Neurobiol 18:633-41. 2008
    ..Both photon-based and electron-based imaging methods will ultimately need highly automated data analysis, because the manual tracing of most networks of interest would require hundreds to tens of thousands of years in human labor...
  8. pmc A dendrite-autonomous mechanism for direction selectivity in retinal starburst amacrine cells
    Susanne E Hausselt
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 5:e185. 2007
    ....
  9. doi request reprint Connectomic reconstruction of the inner plexiform layer in the mouse retina
    Moritz Helmstaedter
    Max Planck Institute for Medical Research, D 69120 Heidelberg, Germany
    Nature 500:168-74. 2013
    ..Circuit motifs that emerge from our data indicate a functional mechanism for a known cellular response in a ganglion cell that detects localized motion, and predict that another ganglion cell is motion sensitive...
  10. ncbi request reprint Directionally selective calcium signals in dendrites of starburst amacrine cells
    Thomas Euler
    Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Nature 418:845-52. 2002
    ..This demonstrates that direction selectivity is computed locally in dendritic branches at a stage before ganglion cells...
  11. pmc High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edge
    Bernd Kuhn
    Department for Biomedical Optics, Max Planck Institute for Medical Research, Heidelberg, Germany
    Biophys J 87:631-9. 2004
    ..Such fractional sensitivities are approximately 5 times larger than what is commonly found with other voltage-sensing dyes and approach the theoretical limit given by the spectral Boltzmann tail...
  12. ncbi request reprint New developments in multiphoton microscopy
    Fritjof Helmchen
    Abt Zellphysiologie, Max Planck Institut fur medizinische Forschung, Jahnstrasse 29, Heidelberg, Germany
    Curr Opin Neurobiol 12:593-601. 2002
    ..In addition, nonlinear optical contrast mechanisms other than multiphoton excitation of fluorescence are being explored...
  13. ncbi request reprint Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier
    Patrick Theer
    Max Planck Institute for Medical Research, Johnstrasse 29, D 69120 Heidelberg, Germany
    Opt Lett 28:1022-4. 2003
    ..The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence...
  14. pmc Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing
    Markus Rueckel
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:17137-42. 2006
    ..CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds...
  15. pmc Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
    Mazahir T Hasan
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 2:e163. 2004
    ....
  16. pmc Eyecup scope--optical recordings of light stimulus-evoked fluorescence signals in the retina
    Thomas Euler
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstr 29, 69120 Heidelberg, Germany
    Pflugers Arch 457:1393-414. 2009
    ..Spatially resolved changes in calcium-indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons...
  17. ncbi request reprint Coherence-gated wave-front sensing in strongly scattering samples
    Marcus Feierabend
    Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Opt Lett 29:2255-7. 2004
    ..Applications are expected for multiphoton and confocal laser-scanning microscopy...
  18. doi request reprint Low-dosage Maximum-A-Posteriori Focusing and Stigmation
    Jonas Binding
    Max Planck Institute for Medical Research, Jahnstr 29, 69120 Heidelberg, Germany
    Microsc Microanal 19:38-55. 2013
    ..A generalization to higher-order aberrations and to other forms of imaging in both two and three dimensions appears possible...
  19. ncbi request reprint On the fundamental imaging-depth limit in two-photon microscopy
    Patrick Theer
    Max Planck Institute for Medical Research, Heidelberg, Germany
    J Opt Soc Am A Opt Image Sci Vis 23:3139-49. 2006
    ....
  20. ncbi request reprint Properties of coherence-gated wavefront sensing
    Markus Rueckel
    Department of Biomedical Optics, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    J Opt Soc Am A Opt Image Sci Vis 24:3517-29. 2007
    ..We show, in particular, that all aberrations needed for adaptive wavefront restoration are correctly sensed if circularly polarized light is used...
  21. ncbi request reprint Targeted whole-cell recordings in the mammalian brain in vivo
    Troy W Margrie
    Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Neuron 39:911-8. 2003
    ..TPTP applied in vivo will therefore provide new insights into the molecular control of neuronal function at the systems level...
  22. doi request reprint Spatiotemporally graded NMDA spike/plateau potentials in basal dendrites of neocortical pyramidal neurons
    Guy Major
    School of Biosciences, Cardiff University, Museum Avenue, Cardiff, Wales, UK
    J Neurophysiol 99:2584-601. 2008
    ..Dendritic NMDA spike/plateaus are thus well-suited to support graded persistent firing...
  23. ncbi request reprint Axons and synaptic boutons are highly dynamic in adult visual cortex
    Dan D Stettler
    The Rockefeller University, New York, New York, USA
    Neuron 49:877-87. 2006
    ..Bouton losses and gains were both approximately 7% of the total population per week, with no net change in the overall density. These results suggest ongoing processes of synaptogenesis and elimination in adult V1...
  24. doi request reprint Imaging in vivo: watching the brain in action
    Jason N D Kerr
    Network Imaging Group, Max Planck Institute for Biological Cybernetics, Spemannstrasse 41, D 72076 Tuebingen, Germany
    Nat Rev Neurosci 9:195-205. 2008
    ..Recently, the limits of what can be achieved in vivo have been pushed into terrain that was previously only accessible in vitro, due to advances in both physical-imaging technology and the design of molecular contrast agents...
  25. ncbi request reprint Two-photon targeted patching (TPTP) in vivo
    Shoji Komai
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916 5, Ikoma, Nara 630 0192, Japan
    Nat Protoc 1:647-52. 2006
    ..This technique may be repeated in the same preparation many times over the course of several hours and is equally applicable to non-neuronal cell types...
  26. ncbi request reprint Contour-propagation algorithms for semi-automated reconstruction of neural processes
    Jakob H Macke
    Max Planck Institute for Biological Cybernetics, Spemannstrasse 38, D 72076 Tubingen, Germany
    J Neurosci Methods 167:349-57. 2008
    ..Compared to purely manual tracing, processing time is greatly accelerated...
  27. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  28. pmc Functional organization of sensory input to the olfactory bulb glomerulus analyzed by two-photon calcium imaging
    Matt Wachowiak
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 101:9097-102. 2004
    ..Hence, sensory inputs to individual glomeruli are spatially heterogeneous but seem to be functionally indiscriminate. These results support the view of olfactory glomeruli as functional units in representing sensory information...
  29. pmc Alpha-herpesvirus infection induces the formation of nuclear actin filaments
    Becket Feierbach
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    PLoS Pathog 2:e85. 2006
    ..Our results suggest a mechanism by which assembly domains are organized within infected cells and provide insight into how the viral infectious cycle and host actin cytoskeleton are integrated to promote the infection process...
  30. ncbi request reprint Towards neural circuit reconstruction with volume electron microscopy techniques
    Kevin L Briggman
    Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Curr Opin Neurobiol 16:562-70. 2006
    ..Such reconstructions of complete neural wiring diagrams could rekindle the tradition of relating neural function to the underlying neuroanatomical circuitry...
  31. pmc The three-dimensional micro- and nanostructure of the aortic medial lamellar unit measured using 3D confocal and electron microscopy imaging
    Mary K O'Connell
    Department of Mechanical Engineering, Stanford University, Stanford, CA, USA
    Matrix Biol 27:171-81. 2008
    ..This high resolution three-dimensional view of the aortic media reveals MLU microstructure details that suggest a highly complex and integrated mural organization that correlates with aortic mechanical properties...
  32. ncbi request reprint Long duration three-dimensional imaging of calcium waves in zebrafish using multiphoton fluorescence microscopy
    Edwin Gilland
    NYU School of Medicine, New York, NY, USA
    Biol Bull 205:176-7. 2003
  33. ncbi request reprint Deep tissue two-photon microscopy
    Fritjof Helmchen
    Department of Neurophysiology, Brain Research Institute, University of Zurich, CH 8057 Zurich, Switzerland
    Nat Methods 2:932-40. 2005
    ..Here we review fundamental concepts of nonlinear microscopy and discuss conditions relevant for achieving large imaging depths in intact tissue...
  34. ncbi request reprint Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo
    Kazuo Kitamura
    Wolfson Institute for Biomedical Research and Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
    Nat Methods 5:61-7. 2008
    ..This approach facilitates the recording, labeling and genetic manipulation of single neurons in the intact native mammalian brain without the need to pre-label neuronal populations...