Max J Cryle
Affiliation: Abteilung Zellphysiologie
Cryle M, Meinhart A, Schlichting I. Structural characterization of OxyD, a cytochrome P450 involved in beta-hydroxytyrosine formation in vancomycin biosynthesis. J Biol Chem. 2010;285:24562-74 pubmed publisher
..The ability to analyze such P450 function based upon sequence data alone should prove an important tool in the analysis and identification of new medicinally relevant biomolecules. ..
Cryle M, Bell S, Schlichting I. Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase. Biochemistry. 2010;49:7282-96 pubmed publisher
..These results represent the first insights into the structural and biochemical basis underlying the multistep oxidation catalyzed by CYP134A1. ..
Cryle M, Staaden J, Schlichting I. Structural characterization of CYP165D3, a cytochrome P450 involved in phenolic coupling in teicoplanin biosynthesis. Arch Biochem Biophys. 2011;507:163-73 pubmed publisher
Brieke C, Kratzig V, Haslinger K, Winkler A, Cryle M. Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Org Biomol Chem. 2015;13:2012-21 pubmed publisher
..These results represent the first important steps in the development of an in vitro biomimetic synthesis of modified glycopeptide aglycones. ..
Brieke C, Peschke M, Haslinger K, Cryle M. Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. Angew Chem Int Ed Engl. 2015;54:15715-9 pubmed publisher
..By using non-natural X-domain substrates, bicyclization of hexapeptides was also shown, which demonstrates the utility of this method for the cyclization of varied peptide substrates inâ€…vitro. ..
Brieke C, Kratzig V, Peschke M, Cryle M. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis. Methods Mol Biol. 2016;1401:85-102 pubmed publisher
..With this, comprehensive investigations of interactions between various NRPS-bound substrates and Cytochrome P450s are enabled. ..
Cryle M, Schlichting I. Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex. Proc Natl Acad Sci U S A. 2008;105:15696-701 pubmed publisher