Automated genome-wide visual profiling of cellular proteins involved in HIV infectionAuguste Genovesio
Institut Pasteur Korea, Gyeonggi Do, Korea
J Biomol Screen 16:945-58. 2011
..As proof of concept, the authors confirmed that silencing of PAK1, Ku70, and RNAseH2A impaired HIV replication in Jurkat cells. In summary, multidimensional, visual profiling can identify genes required for HIV infection...
Visual genome-wide RNAi screening to identify human host factors required for Trypanosoma cruzi infectionAuguste Genovesio
Image Mining Group, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, South Korea
PLoS ONE 6:e19733. 2011
..cruzi infection. This study represents a significant step toward unveiling the key molecular requirements for host cell invasion and revealing new potential targets for antiparasitic therapy...
- Transcription sites are developmentally regulated during the asexual cycle of Plasmodium falciparum
Carolina B Moraes
Center for Neglected Diseases Drug Discovery CND3, Institut Pasteur Korea, Gyeonggi Do, South Korea
PLoS ONE 8:e55539. 2013
..In conclusion, these data suggest that transcription is spatially contained in discrete foci that are developmentally regulated during the asexual cycle of malaria parasites and located in areas of low chromatin density...
- An image-based drug susceptibility assay targeting the placental sequestration of Plasmodium falciparum-infected erythrocytes
Min Je Ku
Center for Neglected Diseases Drug Discovery CND3, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, South Korea
PLoS ONE 7:e41765. 2012
..Taken together, our data establish that the developed assay is highly suitable for drug studies targeting placental malaria, and will facilitate the discovery and rapid development of new therapies against malaria...
- A novel specific edge effect correction method for RNA interference screenings
Jean Philippe Carralot
Biology of Intracellular Pathogens, Inserm Avenir Team, Institut Pasteur Korea, Seongnam si, Korea
Bioinformatics 28:261-8. 2012
..The proposed algorithm was able to correct the edge effect biasing the control data and thus improve assay quality and, consequently, the hit-selection step...
- An image-based high-content screening assay for compounds targeting intracellular Leishmania donovani amastigotes in human macrophages
Jair L Siqueira-Neto
Center for Neglected Diseases Drug Discovery CND3, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, South Korea
PLoS Negl Trop Dis 6:e1671. 2012
..These data corroborate the idea that the intracellular amastigote form of the parasite is the most appropriate to be used in primary screening assay for Leishmania...
- Quantification of protein interaction in living cells by two-photon spectral imaging with fluorescent protein fluorescence resonance energy transfer pair devoid of acceptor bleed-through
Jiho Kim
Neurodegeneration and Applied Microscopy, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, 463 400 South Korea
Cytometry A 81:112-9. 2012
..We believe that the FRET approach using two-photon and fluorescent YFP/mCherry pair is a promising method to monitor protein interaction in living cells...
- A high-content subtractive screen for selecting small molecules affecting internalization of GPCRs
Yong Jun Kwon
Institut Pasteur Korea, Gyeonggi Do, Korea
J Biomol Screen 17:379-85. 2012
..Selective analysis can therefore rapidly select compounds selectively affecting GPCR activity with specificity to one receptor class through high-content screening...
- Contextual automated 3D analysis of subcellular organelles adapted to high-content screening
Thierry Dorval
Cellular Differentiation, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, Korea
J Biomol Screen 15:847-57. 2010
..They show that their method enables the classification of various translocation levels of torsinA and opens the possibility for compound-based screening campaigns restoring the normal torsinA phenotype...
- A modified fluorescence in situ hybridization protocol for Plasmodium falciparum greatly improves nuclear architecture conservation
Monica Contreras-Dominguez
Center for Neglected Diseases Drug Discovery, Institut Pasteur Korea, Bundang gu, Seongnam si, Gyeonggi Do, South Korea
Mol Biochem Parasitol 173:48-52. 2010
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High-content classification of nucleocytoplasmic import or export inhibitorsYong Jun Kwon
Institut Pasteur Korea, Seongbuk gu, Seoul, Korea
J Biomol Screen 12:621-7. 2007
..The results establish a robust method for identifying compounds regulating nucleocytoplasmic import or export and also implicate MAP kinases in nuclear import of nuclear factor kappa B...
