Kariawasam R, Touma C, Cubeddu L, Gamsjaeger R. Backbone (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface. Biomol NMR Assign. 2016;10:297-300 pubmed publisher
..Our NMR data in combination with further biophysical and biochemical experiments will allow us to address these discrepancies and shed light onto the structural basis of DNA-binding by hSSB1 in solution. ..
Croft L, Bolderson E, Adams M, El Kamand S, Kariawasam R, Cubeddu L, et al
. Human single-stranded DNA binding protein 1 (hSSB1, OBFC2B), a critical component of the DNA damage response. Semin Cell Dev Biol. 2019;86:121-128 pubmed publisher
..While RPA is extremely well characterised, the roles of hSSB1 and hSSB2 have only emerged recently. In this review, we discuss the critical roles that hSSB1 plays in the maintenance of genomic stability. ..
Touma C, Kariawasam R, Gimenez A, Bernardo R, Ashton N, Adams M, et al
. A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution. Nucleic Acids Res. 2016;44:7963-73 pubmed publisher
..Our findings elucidate the detailed molecular mechanism in solution of ssDNA binding by hSSB1, a major player in the maintenance of genomic stability. ..
Ashton N, Paquet N, Shirran S, Bolderson E, Kariawasam R, Touma C, et al
. hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases. DNA Repair (Amst). 2017;54:30-39 pubmed publisher
..Together, these data thereby represent a novel mechanism for hSSB1 regulation following the inhibition of replication. ..
Kariawasam R, Knight M, Gamsjaeger R, Cubeddu L. Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB2 (NABP1/OBFC2A) and chemical shift mapping of the DNA-binding interface. Biomol NMR Assign. 2018;12:107-111 pubmed publisher
..Future studies will aim at determining the structural basis of these differences and thus help to gain a more comprehensive understanding of the functional divergences that these novel hSSBs display in the context of genome maintenance. ..